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Gene information
General information
TC007655 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1434931..1444351
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007655
    iBeetle screen iB_04359

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007655 RA
      MATREKKLS ISRKQNTSS PLPPKDDLT PPEIKPPPN 
      NDTAEETPA PKQSPKAPS LAKKLAPKT PKKLTKKPT 
      IAIKLKPAI KKNVKIKRK IIKVIGNNP KNAETKKKL 
      LKSCVKQIK PKKAKVEKS EETLPPVLE PILPIEEDA 
      DNKQVRKKG GRAPRIDNY AKNELLVDP AKSKRVYKK 
      KIKAENSES IEDVMNDLN SLIKGESKE ESDVVSESG 
      KSDSLKAKK IVKRRKKEN IENTLSKLS PSQRKVIDM 
      LDLNVSKFK DLPVVKRRH SIEKFPIGI VDTDGPNLN 
      RLSIFNNLP RCISPRSKN FPRLRQTVD GFNRRSNPY 
      KTRSDSPAR ILRNGKQRK LKDLELLEG LDITHKRRR 
      RNYSEFSGS EMSKFSGYE SDSSFSDLS SVHGAENPE 
      TKDMDGIKK EKIEDDGNP PAVSAETDH NFTDTNSNL 
      CLNKDVSVK DERDVLDSM PSAPSSKVP EKSIILDIM 
      KQAFNDPFE EDKQKKKTS VEDVKIEPT TENSEDKNQ 
      EEVVENGET VTEEVANGE VISNGDAQI EETAENLAI 
      KENILQALG LQSLKAAKE ARLKSKEKA EPKSDLYTG 
      TLKTVIKIN RGEKKKGRN MKMTLHKTK KSECDNGDD 
      HKLKEGPST SWKHGGQSS ESAGAHRKS HYSNRSNMD 
      GSSEHTSDG ETGAQEGST KALVIPEKA SSFSIHPGR 
      ICKDECSYC YGKFGLFDT PCHIAQMKS VERQNKILA 
      AEKHLTRDS CLCDACYRH VDRKANTPS YTNKSYKRS 
      ATVAPGPRQ DHCHVLGCR KVATNILRR KWIIKMRRS 
      ICEVINIDL DNPGLHSIP ICDEHYSAL EYLMVCAMC 
      KRRLAKNHV HYLGPETVD LNLALTTEG IPLSLTDKP 
      VVCKLCKYF ATIILKPPE DRPENSSTF FVEYKKRLL 
      HFNDIVPMD EAAAEEPII IPTKDSKIE KEQVRKKRK 
      ISKNQSVPG DGSDSQVEV EKSDQSRVP STDSTNQSR 
      PESPSDYMV DYNTLIPSI AMECGSDSE SKKEQPPKP 
      THLKKVGRP ETIKQAVEI SKNMKLKND KSSDIAVQR 
      LGSNPSISV RQLFPCEED LPLQGTIEF GNVKERTPE 
      GWEKCTSVI QYDMETKHL WQELQKPYG NQSSFLRHL 
      ILLEKYFRN GDLILAPNA SHHSINYSE SVHNRLRAY 
      DNIPSSAGN VQPISMIPF NKVQKSTNG IVTTANTNP 
      AKEPPPAVP PTINAANVP KSTPITISQ LNSPPLIPP 
      NPPPPKPKP VGAPPGLIS LHPGTNRPV APLVKAPQS 
      QKIKFPITK NWRPNLIPI DPSKKNSER KAGLVQVIS 
      GGKPYHITL EDYKKMCAI KKSFELKQK RLQEAAKGA 
      QTSVLKAVT PRKSLMISK GKAEESETI LDKLDKQVE 
      KLESRLNEN KPPLSLPKI PKSLTVIPQ TVTRKPSRP 
      SSPVLLITP KPKQIANKS
    CDS sequence
    • >TC007655 RA
      atggcaact cgagaaaag aagctttca attagccga 
      aagcaaaac acatcgtct cctttaccg ccaaaggac 
      gatctaaca cctcctgaa ataaaacct cccccaaac 
      aatgacaca gcggaagaa acacccgct ccaaagcaa 
      tcaccgaaa gccccatct ttagcaaaa aaactggca 
      ccaaaaacc ccaaagaaa ctaacaaag aaaccaacc 
      atcgcgatc aaactaaaa cccgcgatt aagaaaaac 
      gtgaaaatt aaacgaaaa atcatcaaa gtgataggc 
      aataacccg aaaaatgct gaaactaaa aagaaattg 
      ctcaagagt tgcgtcaag caaatcaaa ccgaaaaaa 
      gcaaaagtt gagaaaagc gaagagacg ttaccccct 
      gttctggaa ccgattcta ccaattgaa gaggacgct 
      gataacaaa caagtcagg aaaaaaggc ggacgcgcg 
      ccacgaatc gacaattac gccaaaaac gaactatta 
      gtcgatccc gcaaaaagc aaacgcgtc tacaagaaa 
      aaaatcaaa gctgaaaac agcgaatcg attgaagac 
      gtcatgaac gatctgaat tcgctcatc aaaggcgag 
      agtaaagag gaatccgac gtggtgtca gaatcgggc 
      aaaagcgac tccctaaag gcgaaaaaa atcgtcaaa 
      agacgcaag aaagaaaac attgagaac accctaagc 
      aagctgagt ccttcccag cgcaaagta atcgacatg 
      ctggacttg aacgtcagc aaattcaag gacttgcca 
      gtggttaaa aggcgccac agcattgaa aaattcccg 
      attgggatt gtagacacc gatggacca aacctcaac 
      cgactgtca attttcaat aacttacct cgctgtatt 
      agccctcgt tcgaaaaat ttccctagg ttaaggcaa 
      acagtggat gggttcaac cgccggtcg aatccttac 
      aagaccagg tcggactca ccggcccgc attttgcgg 
      aatggaaaa cagaggaaa ctgaaagat ttggaactg 
      ctggaaggt ctggacatt acacacaag cggcgaaga 
      cggaactat tccgagttc agcggcagc gaaatgtcc 
      aagttttca ggctacgag tccgatagt agcttctcg 
      gacttgtcg tcagtacac ggtgctgaa aatcccgaa 
      acgaaggac atggacggc atcaagaag gagaaaatt 
      gaagacgat ggcaatcct cccgccgtt tcggctgaa 
      accgaccac aatttcacc gacaccaac tcgaatctt 
      tgtctgaat aaggacgtt tcggttaag gacgaacga 
      gacgtcctg gacagcatg ccatcggcc cccagctcc 
      aaagttcct gaaaaatcg ataatcctc gacatcatg 
      aagcaagcc ttcaatgat cccttcgag gaagacaaa 
      cagaaaaag aagacttct gtcgaagac gtgaaaatc 
      gaaccaaca accgaaaat tccgaagat aaaaaccaa 
      gaggaagtg gtcgaaaat ggcgaaact gtgactgaa 
      gaagtcgcc aatggcgaa gttatctca aacggcgac 
      gcacaaatt gaagaaacc gccgaaaac ctcgcgatc 
      aaagaaaac atcctgcaa gctttaggc ttgcaaagt 
      ttgaaggca gctaaggaa gcgcgactg aagagcaag 
      gaaaaggcc gaacccaag agcgacctg tacaccggg 
      acgctcaaa accgtgatc aaaatcaac cgcggcgag 
      aagaagaaa ggtaggaac atgaagatg actttgcac 
      aaaaccaag aaaagcgag tgtgataac ggcgacgac 
      cacaaatta aaagaaggg ccgtccaca agctggaaa 
      catggtggt cagtcgtcg gaatcggcc ggtgcgcac 
      agaaaatca cattattct aaccgctct aacatggat 
      ggcagcagt gagcacacg tccgatggc gagaccggg 
      gcgcaggaa ggatcgacc aaagctttg gtcataccg 
      gagaaagct agttccttc agtatacac ccggggagg 
      atctgcaag gacgaatgt tcctattgc tacggcaag 
      ttcggactg ttcgatact ccgtgtcat atcgcacaa 
      atgaagagc gtcgaaagg cagaataaa attctagcc 
      gccgaaaaa catctaacg cgcgattct tgcctttgt 
      gacgcttgt tatcgccac gttgaccgt aaggccaac 
      acgccctct tacacgaac aaatcttac aagagaagt 
      gcaaccgtt gcaccagga cctagacag gatcactgc 
      cacgtgttg gggtgtcgt aaagtcgcc acaaatata 
      ctgcggagg aagtggatc attaaaatg aggaggagt 
      atatgcgaa gtgataaac atagattta gataatcct 
      ggtcttcat tctattcct atttgtgat gagcattac 
      tcggccctt gaatacctc atggtgtgt gctatgtgt 
      aaaagaaga ttagctaag aatcacgtt cattatttg 
      ggacctgaa accgtcgat ttaaactta gctttgacc 
      acggaagga attccctta agtttaacc gataaaccg 
      gtcgtttgc aaactgtgt aaatatttc gctacgatt 
      attctcaaa ccgcctgaa gatcggccc gaaaacagc 
      tcaaccttt tttgttgaa tacaagaag aggctgctt 
      catttcaac gatatcgtc ccgatggat gaagcggcg 
      gccgaagag ccgataata attcccaca aaggacagc 
      aaaatcgaa aaagagcaa gtccggaag aaacggaag 
      atatcaaag aaccagtca gtgcccgga gacggttca 
      gattctcag gtagaagta gaaaaatcg gaccaaagt 
      cgtgtgcct tcaaccgat tcaacaaac caatcacgt 
      ccagaatct ccctctgac tacatggtg gactacaac 
      actttaatt ccatccata gcaatggaa tgtggcagt 
      gatagtgaa tctaaaaaa gaacaacca ccaaaacca 
      acccatttg aagaaagtc ggacgtcca gaaaccatc 
      aaacaagca gtggaaata tcaaagaac atgaaactg 
      aaaaacgac aaaagtagt gatatcgcg gtccaaaga 
      ctaggatca aatccttcc atatcagtc cgacaattg 
      ttcccttgc gaggaagat cttccactt cagggcacc 
      atagaattc ggcaacgtc aaagaacgg acgcccgaa 
      ggctgggag aagtgcacc tcagtcata caatacgac 
      atggaaacc aaacactta tggcaagaa ttgcaaaaa 
      ccatacgga aaccaaagc agcttcctg cgacattta 
      atcctcctg gagaaatat ttccgcaac ggtgattta 
      atcctagca cccaacgct agccaccat tcaatcaac 
      tacagcgaa tcggtccac aaccgcctc cgcgcctac 
      gacaacatc ccatcaagc gctggcaac gtccaaccc 
      atcagcatg atcccattc aacaaagtc cagaaaagt 
      accaacgga atcgtcacc accgccaac acaaacccg 
      gccaaagaa cccccacct gcagtccca ccaaccata 
      aacgcagca aacgtcccc aaaagcacc cccattaca 
      atatcccag ctaaacagc cccccttta atcccacct 
      aacccccca ccccccaaa cccaagccc gtgggcgcc 
      cccccaggc ctcatctcc ctgcaccct ggcaccaac 
      cggccagtg gccccccta gtcaaagcc ccccaatcc 
      cagaaaatc aaattcccg ataacgaaa aactggcga 
      ccgaattta atcccaatc gacccttcg aagaaaaac 
      tccgaacgc aaagccggc ttggtgcag gtgatttcg 
      ggcgggaaa ccctaccat atcactcta gaagactac 
      aaaaaaatg tgtgctatc aagaagagt ttcgagctg 
      aagcagaag cgactccag gaggcggca aaaggggcg 
      caaacgtcg gtgctaaag gcggtaacg ccgcgcaaa 
      agcttgatg atttccaag gggaaggcc gaagagtcg 
      gagacgatt ttagacaag ttggacaag caagtggaa 
      aagttagaa tcgagactg aacgaaaat aaaccgccg 
      ttatcactt ccgaaaata cctaaatcg ttgacggtt 
      atcccgcag acggtgact cggaaaccc tcccggcct 
      tcaagtccc gttctacta attaccccc aaaccaaaa 
      cagatagca aacaagtca tga
    mRNA sequence
    • >TC007655 RA
      aagaaccac gtccctatc gtgacaagc caaagtata 
      tctaatagt tcggacata ccgccgcag ttcccaaag 
      ttttcccgc gtttccggc ccgaatttt ccgcatttt 
      cccgaacat ttcgccgga ttcggcgcc gccacgacc 
      ggaaatcgc gaaaaccgc ccaaaaacg cccggatta 
      tcctcgaat ttcgcaata aattgagtc gaatccgtc 
      ggttgctct ggtgcaaac atgtgcatc gtttagcct 
      tcggccggc ttcgggctc cttcggcgc cttctacgt 
      gcttttggt ggaatagcg acgacgcac atcgcatag 
      gtggcgttg tgcaccacg ttcagggaa gaattttgt 
      aatgacatg gcaactcga gaaaagaag ctttcaatt 
      agccgaaag caaaacaca tcgtctcct ttaccgcca 
      aaggacgat ctaacacct cctgaaata aaacctccc 
      ccaaacaat gacacagcg gaagaaaca cccgctcca 
      aagcaatca ccgaaagcc ccatcttta gcaaaaaaa 
      ctggcacca aaaacccca aagaaacta acaaagaaa 
      ccaaccatc gcgatcaaa ctaaaaccc gcgattaag 
      aaaaacgtg aaaattaaa cgaaaaatc atcaaagtg 
      ataggcaat aacccgaaa aatgctgaa actaaaaag 
      aaattgctc aagagttgc gtcaagcaa atcaaaccg 
      aaaaaagca aaagttgag aaaagcgaa gagacgtta 
      ccccctgtt ctggaaccg attctacca attgaagag 
      gacgctgat aacaaacaa gtcaggaaa aaaggcgga 
      cgcgcgcca cgaatcgac aattacgcc aaaaacgaa 
      ctattagtc gatcccgca aaaagcaaa cgcgtctac 
      aagaaaaaa atcaaagct gaaaacagc gaatcgatt 
      gaagacgtc atgaacgat ctgaattcg ctcatcaaa 
      ggcgagagt aaagaggaa tccgacgtg gtgtcagaa 
      tcgggcaaa agcgactcc ctaaaggcg aaaaaaatc 
      gtcaaaaga cgcaagaaa gaaaacatt gagaacacc 
      ctaagcaag ctgagtcct tcccagcgc aaagtaatc 
      gacatgctg gacttgaac gtcagcaaa ttcaaggac 
      ttgccagtg gttaaaagg cgccacagc attgaaaaa 
      ttcccgatt gggattgta gacaccgat ggaccaaac 
      ctcaaccga ctgtcaatt ttcaataac ttacctcgc 
      tgtattagc cctcgttcg aaaaatttc cctaggtta 
      aggcaaaca gtggatggg ttcaaccgc cggtcgaat 
      ccttacaag accaggtcg gactcaccg gcccgcatt 
      ttgcggaat ggaaaacag aggaaactg aaagatttg 
      gaactgctg gaaggtctg gacattaca cacaagcgg 
      cgaagacgg aactattcc gagttcagc ggcagcgaa 
      atgtccaag ttttcaggc tacgagtcc gatagtagc 
      ttctcggac ttgtcgtca gtacacggt gctgaaaat 
      cccgaaacg aaggacatg gacggcatc aagaaggag 
      aaaattgaa gacgatggc aatcctccc gccgtttcg 
      gctgaaacc gaccacaat ttcaccgac accaactcg 
      aatctttgt ctgaataag gacgtttcg gttaaggac 
      gaacgagac gtcctggac agcatgcca tcggccccc 
      agctccaaa gttcctgaa aaatcgata atcctcgac 
      atcatgaag caagccttc aatgatccc ttcgaggaa 
      gacaaacag aaaaagaag acttctgtc gaagacgtg 
      aaaatcgaa ccaacaacc gaaaattcc gaagataaa 
      aaccaagag gaagtggtc gaaaatggc gaaactgtg 
      actgaagaa gtcgccaat ggcgaagtt atctcaaac 
      ggcgacgca caaattgaa gaaaccgcc gaaaacctc 
      gcgatcaaa gaaaacatc ctgcaagct ttaggcttg 
      caaagtttg aaggcagct aaggaagcg cgactgaag 
      agcaaggaa aaggccgaa cccaagagc gacctgtac 
      accgggacg ctcaaaacc gtgatcaaa atcaaccgc 
      ggcgagaag aagaaaggt aggaacatg aagatgact 
      ttgcacaaa accaagaaa agcgagtgt gataacggc 
      gacgaccac aaattaaaa gaagggccg tccacaagc 
      tggaaacat ggtggtcag tcgtcggaa tcggccggt 
      gcgcacaga aaatcacat tattctaac cgctctaac 
      atggatggc agcagtgag cacacgtcc gatggcgag 
      accggggcg caggaagga tcgaccaaa gctttggtc 
      ataccggag aaagctagt tccttcagt atacacccg 
      gggaggatc tgcaaggac gaatgttcc tattgctac 
      ggcaagttc ggactgttc gatactccg tgtcatatc 
      gcacaaatg aagagcgtc gaaaggcag aataaaatt 
      ctagccgcc gaaaaacat ctaacgcgc gattcttgc 
      ctttgtgac gcttgttat cgccacgtt gaccgtaag 
      gccaacacg ccctcttac acgaacaaa tcttacaag 
      agaagtgca accgttgca ccaggacct agacaggat 
      cactgccac gtgttgggg tgtcgtaaa gtcgccaca 
      aatatactg cggaggaag tggatcatt aaaatgagg 
      aggagtata tgcgaagtg ataaacata gatttagat 
      aatcctggt cttcattct attcctatt tgtgatgag 
      cattactcg gcccttgaa tacctcatg gtgtgtgct 
      atgtgtaaa agaagatta gctaagaat cacgttcat 
      tatttggga cctgaaacc gtcgattta aacttagct 
      ttgaccacg gaaggaatt cccttaagt ttaaccgat 
      aaaccggtc gtttgcaaa ctgtgtaaa tatttcgct 
      acgattatt ctcaaaccg cctgaagat cggcccgaa 
      aacagctca acctttttt gttgaatac aagaagagg 
      ctgcttcat ttcaacgat atcgtcccg atggatgaa 
      gcggcggcc gaagagccg ataataatt cccacaaag 
      gacagcaaa atcgaaaaa gagcaagtc cggaagaaa 
      cggaagata tcaaagaac cagtcagtg cccggagac 
      ggttcagat tctcaggta gaagtagaa aaatcggac 
      caaagtcgt gtgccttca accgattca acaaaccaa 
      tcacgtcca gaatctccc tctgactac atggtggac 
      tacaacact ttaattcca tccatagca atggaatgt 
      ggcagtgat agtgaatct aaaaaagaa caaccacca 
      aaaccaacc catttgaag aaagtcgga cgtccagaa 
      accatcaaa caagcagtg gaaatatca aagaacatg 
      aaactgaaa aacgacaaa agtagtgat atcgcggtc 
      caaagacta ggatcaaat ccttccata tcagtccga 
      caattgttc ccttgcgag gaagatctt ccacttcag 
      ggcaccata gaattcggc aacgtcaaa gaacggacg 
      cccgaaggc tgggagaag tgcacctca gtcatacaa 
      tacgacatg gaaaccaaa cacttatgg caagaattg 
      caaaaacca tacggaaac caaagcagc ttcctgcga 
      catttaatc ctcctggag aaatatttc cgcaacggt 
      gatttaatc ctagcaccc aacgctagc caccattca 
      atcaactac agcgaatcg gtccacaac cgcctccgc 
      gcctacgac aacatccca tcaagcgct ggcaacgtc 
      caacccatc agcatgatc ccattcaac aaagtccag 
      aaaagtacc aacggaatc gtcaccacc gccaacaca 
      aacccggcc aaagaaccc ccacctgca gtcccacca 
      accataaac gcagcaaac gtccccaaa agcaccccc 
      attacaata tcccagcta aacagcccc cctttaatc 
      ccacctaac cccccaccc cccaaaccc aagcccgtg 
      ggcgccccc ccaggcctc atctccctg caccctggc 
      accaaccgg ccagtggcc cccctagtc aaagccccc 
      caatcccag aaaatcaaa ttcccgata acgaaaaac 
      tggcgaccg aatttaatc ccaatcgac ccttcgaag 
      aaaaactcc gaacgcaaa gccggcttg gtgcaggtg 
      atttcgggc gggaaaccc taccatatc actctagaa 
      gactacaaa aaaatgtgt gctatcaag aagagtttc 
      gagctgaag cagaagcga ctccaggag gcggcaaaa 
      ggggcgcaa acgtcggtg ctaaaggcg gtaacgccg 
      cgcaaaagc ttgatgatt tccaagggg aaggccgaa 
      gagtcggag acgatttta gacaagttg gacaagcaa 
      gtggaaaag ttagaatcg agactgaac gaaaataaa 
      ccgccgtta tcacttccg aaaatacct aaatcgttg 
      acggttatc ccgcagacg gtgactcgg aaaccctcc 
      cggccttca agtcccgtt ctactaatt acccccaaa 
      ccaaaacag atagcaaac aagtcatga ttaattctg 
      acgtgtttt acaaagtgc ctcaaagaa atcgtcttt 
      tgtacgcta gaagagaga ttgaatata tcgacagtt 
      ttgtttttt attttatgc aaataaatt gttggcgga 
      attgtaagt ttgctttga ttatattgt tacgagatt 
      gacacgagt tagaggata tttttttat gtaacttgt 
      aaatatccc aatccactc ttaaacgac gtatgtgtt 
      cagtagctg catacatga tttcgtttg gtcaaaatt 
      ccagcaata acggtcgtc tttccatta ccaatttat 
      agtttcgag tacttagaa agaagggaa aatgtacaa 
      acagtgcga gtgtttgca cgtttttcg gttccaatg 
      ctcattaat catttaatt gtaatccta atttattac 
      agttttagt atttttgta tacctatgt ttagttgga 
      tgttattgg cttgtaatg atttgtgcc ggtcagatg 
      atcggctag aatgtgaaa ttgaaaagt ctgaaatat 
      tgttaattt ttctgaaac ctgtataaa gcccactat 
      ttgaagtta ctagttaat tcattataa ttatttatg 
      tacataaac cgggcgtta caataattg aaaataaat 
      aatttaata catt
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