| General information | |||
| TC007655 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 1434931..1444351 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007655 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04359 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
- >TC007655 RA
MATREKKLS ISRKQNTSS PLPPKDDLT PPEIKPPPN NDTAEETPA PKQSPKAPS LAKKLAPKT PKKLTKKPT IAIKLKPAI KKNVKIKRK IIKVIGNNP KNAETKKKL LKSCVKQIK PKKAKVEKS EETLPPVLE PILPIEEDA DNKQVRKKG GRAPRIDNY AKNELLVDP AKSKRVYKK KIKAENSES IEDVMNDLN SLIKGESKE ESDVVSESG KSDSLKAKK IVKRRKKEN IENTLSKLS PSQRKVIDM LDLNVSKFK DLPVVKRRH SIEKFPIGI VDTDGPNLN RLSIFNNLP RCISPRSKN FPRLRQTVD GFNRRSNPY KTRSDSPAR ILRNGKQRK LKDLELLEG LDITHKRRR RNYSEFSGS EMSKFSGYE SDSSFSDLS SVHGAENPE TKDMDGIKK EKIEDDGNP PAVSAETDH NFTDTNSNL CLNKDVSVK DERDVLDSM PSAPSSKVP EKSIILDIM KQAFNDPFE EDKQKKKTS VEDVKIEPT TENSEDKNQ EEVVENGET VTEEVANGE VISNGDAQI EETAENLAI KENILQALG LQSLKAAKE ARLKSKEKA EPKSDLYTG TLKTVIKIN RGEKKKGRN MKMTLHKTK KSECDNGDD HKLKEGPST SWKHGGQSS ESAGAHRKS HYSNRSNMD GSSEHTSDG ETGAQEGST KALVIPEKA SSFSIHPGR ICKDECSYC YGKFGLFDT PCHIAQMKS VERQNKILA AEKHLTRDS CLCDACYRH VDRKANTPS YTNKSYKRS ATVAPGPRQ DHCHVLGCR KVATNILRR KWIIKMRRS ICEVINIDL DNPGLHSIP ICDEHYSAL EYLMVCAMC KRRLAKNHV HYLGPETVD LNLALTTEG IPLSLTDKP VVCKLCKYF ATIILKPPE DRPENSSTF FVEYKKRLL HFNDIVPMD EAAAEEPII IPTKDSKIE KEQVRKKRK ISKNQSVPG DGSDSQVEV EKSDQSRVP STDSTNQSR PESPSDYMV DYNTLIPSI AMECGSDSE SKKEQPPKP THLKKVGRP ETIKQAVEI SKNMKLKND KSSDIAVQR LGSNPSISV RQLFPCEED LPLQGTIEF GNVKERTPE GWEKCTSVI QYDMETKHL WQELQKPYG NQSSFLRHL ILLEKYFRN GDLILAPNA SHHSINYSE SVHNRLRAY DNIPSSAGN VQPISMIPF NKVQKSTNG IVTTANTNP AKEPPPAVP PTINAANVP KSTPITISQ LNSPPLIPP NPPPPKPKP VGAPPGLIS LHPGTNRPV APLVKAPQS QKIKFPITK NWRPNLIPI DPSKKNSER KAGLVQVIS GGKPYHITL EDYKKMCAI KKSFELKQK RLQEAAKGA QTSVLKAVT PRKSLMISK GKAEESETI LDKLDKQVE KLESRLNEN KPPLSLPKI PKSLTVIPQ TVTRKPSRP SSPVLLITP KPKQIANKS
- >TC007655 RA
atggcaact cgagaaaag aagctttca attagccga aagcaaaac acatcgtct cctttaccg ccaaaggac gatctaaca cctcctgaa ataaaacct cccccaaac aatgacaca gcggaagaa acacccgct ccaaagcaa tcaccgaaa gccccatct ttagcaaaa aaactggca ccaaaaacc ccaaagaaa ctaacaaag aaaccaacc atcgcgatc aaactaaaa cccgcgatt aagaaaaac gtgaaaatt aaacgaaaa atcatcaaa gtgataggc aataacccg aaaaatgct gaaactaaa aagaaattg ctcaagagt tgcgtcaag caaatcaaa ccgaaaaaa gcaaaagtt gagaaaagc gaagagacg ttaccccct gttctggaa ccgattcta ccaattgaa gaggacgct gataacaaa caagtcagg aaaaaaggc ggacgcgcg ccacgaatc gacaattac gccaaaaac gaactatta gtcgatccc gcaaaaagc aaacgcgtc tacaagaaa aaaatcaaa gctgaaaac agcgaatcg attgaagac gtcatgaac gatctgaat tcgctcatc aaaggcgag agtaaagag gaatccgac gtggtgtca gaatcgggc aaaagcgac tccctaaag gcgaaaaaa atcgtcaaa agacgcaag aaagaaaac attgagaac accctaagc aagctgagt ccttcccag cgcaaagta atcgacatg ctggacttg aacgtcagc aaattcaag gacttgcca gtggttaaa aggcgccac agcattgaa aaattcccg attgggatt gtagacacc gatggacca aacctcaac cgactgtca attttcaat aacttacct cgctgtatt agccctcgt tcgaaaaat ttccctagg ttaaggcaa acagtggat gggttcaac cgccggtcg aatccttac aagaccagg tcggactca ccggcccgc attttgcgg aatggaaaa cagaggaaa ctgaaagat ttggaactg ctggaaggt ctggacatt acacacaag cggcgaaga cggaactat tccgagttc agcggcagc gaaatgtcc aagttttca ggctacgag tccgatagt agcttctcg gacttgtcg tcagtacac ggtgctgaa aatcccgaa acgaaggac atggacggc atcaagaag gagaaaatt gaagacgat ggcaatcct cccgccgtt tcggctgaa accgaccac aatttcacc gacaccaac tcgaatctt tgtctgaat aaggacgtt tcggttaag gacgaacga gacgtcctg gacagcatg ccatcggcc cccagctcc aaagttcct gaaaaatcg ataatcctc gacatcatg aagcaagcc ttcaatgat cccttcgag gaagacaaa cagaaaaag aagacttct gtcgaagac gtgaaaatc gaaccaaca accgaaaat tccgaagat aaaaaccaa gaggaagtg gtcgaaaat ggcgaaact gtgactgaa gaagtcgcc aatggcgaa gttatctca aacggcgac gcacaaatt gaagaaacc gccgaaaac ctcgcgatc aaagaaaac atcctgcaa gctttaggc ttgcaaagt ttgaaggca gctaaggaa gcgcgactg aagagcaag gaaaaggcc gaacccaag agcgacctg tacaccggg acgctcaaa accgtgatc aaaatcaac cgcggcgag aagaagaaa ggtaggaac atgaagatg actttgcac aaaaccaag aaaagcgag tgtgataac ggcgacgac cacaaatta aaagaaggg ccgtccaca agctggaaa catggtggt cagtcgtcg gaatcggcc ggtgcgcac agaaaatca cattattct aaccgctct aacatggat ggcagcagt gagcacacg tccgatggc gagaccggg gcgcaggaa ggatcgacc aaagctttg gtcataccg gagaaagct agttccttc agtatacac ccggggagg atctgcaag gacgaatgt tcctattgc tacggcaag ttcggactg ttcgatact ccgtgtcat atcgcacaa atgaagagc gtcgaaagg cagaataaa attctagcc gccgaaaaa catctaacg cgcgattct tgcctttgt gacgcttgt tatcgccac gttgaccgt aaggccaac acgccctct tacacgaac aaatcttac aagagaagt gcaaccgtt gcaccagga cctagacag gatcactgc cacgtgttg gggtgtcgt aaagtcgcc acaaatata ctgcggagg aagtggatc attaaaatg aggaggagt atatgcgaa gtgataaac atagattta gataatcct ggtcttcat tctattcct atttgtgat gagcattac tcggccctt gaatacctc atggtgtgt gctatgtgt aaaagaaga ttagctaag aatcacgtt cattatttg ggacctgaa accgtcgat ttaaactta gctttgacc acggaagga attccctta agtttaacc gataaaccg gtcgtttgc aaactgtgt aaatatttc gctacgatt attctcaaa ccgcctgaa gatcggccc gaaaacagc tcaaccttt tttgttgaa tacaagaag aggctgctt catttcaac gatatcgtc ccgatggat gaagcggcg gccgaagag ccgataata attcccaca aaggacagc aaaatcgaa aaagagcaa gtccggaag aaacggaag atatcaaag aaccagtca gtgcccgga gacggttca gattctcag gtagaagta gaaaaatcg gaccaaagt cgtgtgcct tcaaccgat tcaacaaac caatcacgt ccagaatct ccctctgac tacatggtg gactacaac actttaatt ccatccata gcaatggaa tgtggcagt gatagtgaa tctaaaaaa gaacaacca ccaaaacca acccatttg aagaaagtc ggacgtcca gaaaccatc aaacaagca gtggaaata tcaaagaac atgaaactg aaaaacgac aaaagtagt gatatcgcg gtccaaaga ctaggatca aatccttcc atatcagtc cgacaattg ttcccttgc gaggaagat cttccactt cagggcacc atagaattc ggcaacgtc aaagaacgg acgcccgaa ggctgggag aagtgcacc tcagtcata caatacgac atggaaacc aaacactta tggcaagaa ttgcaaaaa ccatacgga aaccaaagc agcttcctg cgacattta atcctcctg gagaaatat ttccgcaac ggtgattta atcctagca cccaacgct agccaccat tcaatcaac tacagcgaa tcggtccac aaccgcctc cgcgcctac gacaacatc ccatcaagc gctggcaac gtccaaccc atcagcatg atcccattc aacaaagtc cagaaaagt accaacgga atcgtcacc accgccaac acaaacccg gccaaagaa cccccacct gcagtccca ccaaccata aacgcagca aacgtcccc aaaagcacc cccattaca atatcccag ctaaacagc cccccttta atcccacct aacccccca ccccccaaa cccaagccc gtgggcgcc cccccaggc ctcatctcc ctgcaccct ggcaccaac cggccagtg gccccccta gtcaaagcc ccccaatcc cagaaaatc aaattcccg ataacgaaa aactggcga ccgaattta atcccaatc gacccttcg aagaaaaac tccgaacgc aaagccggc ttggtgcag gtgatttcg ggcgggaaa ccctaccat atcactcta gaagactac aaaaaaatg tgtgctatc aagaagagt ttcgagctg aagcagaag cgactccag gaggcggca aaaggggcg caaacgtcg gtgctaaag gcggtaacg ccgcgcaaa agcttgatg atttccaag gggaaggcc gaagagtcg gagacgatt ttagacaag ttggacaag caagtggaa aagttagaa tcgagactg aacgaaaat aaaccgccg ttatcactt ccgaaaata cctaaatcg ttgacggtt atcccgcag acggtgact cggaaaccc tcccggcct tcaagtccc gttctacta attaccccc aaaccaaaa cagatagca aacaagtca tga
- >TC007655 RA
aagaaccac gtccctatc gtgacaagc caaagtata tctaatagt tcggacata ccgccgcag ttcccaaag ttttcccgc gtttccggc ccgaatttt ccgcatttt cccgaacat ttcgccgga ttcggcgcc gccacgacc ggaaatcgc gaaaaccgc ccaaaaacg cccggatta tcctcgaat ttcgcaata aattgagtc gaatccgtc ggttgctct ggtgcaaac atgtgcatc gtttagcct tcggccggc ttcgggctc cttcggcgc cttctacgt gcttttggt ggaatagcg acgacgcac atcgcatag gtggcgttg tgcaccacg ttcagggaa gaattttgt aatgacatg gcaactcga gaaaagaag ctttcaatt agccgaaag caaaacaca tcgtctcct ttaccgcca aaggacgat ctaacacct cctgaaata aaacctccc ccaaacaat gacacagcg gaagaaaca cccgctcca aagcaatca ccgaaagcc ccatcttta gcaaaaaaa ctggcacca aaaacccca aagaaacta acaaagaaa ccaaccatc gcgatcaaa ctaaaaccc gcgattaag aaaaacgtg aaaattaaa cgaaaaatc atcaaagtg ataggcaat aacccgaaa aatgctgaa actaaaaag aaattgctc aagagttgc gtcaagcaa atcaaaccg aaaaaagca aaagttgag aaaagcgaa gagacgtta ccccctgtt ctggaaccg attctacca attgaagag gacgctgat aacaaacaa gtcaggaaa aaaggcgga cgcgcgcca cgaatcgac aattacgcc aaaaacgaa ctattagtc gatcccgca aaaagcaaa cgcgtctac aagaaaaaa atcaaagct gaaaacagc gaatcgatt gaagacgtc atgaacgat ctgaattcg ctcatcaaa ggcgagagt aaagaggaa tccgacgtg gtgtcagaa tcgggcaaa agcgactcc ctaaaggcg aaaaaaatc gtcaaaaga cgcaagaaa gaaaacatt gagaacacc ctaagcaag ctgagtcct tcccagcgc aaagtaatc gacatgctg gacttgaac gtcagcaaa ttcaaggac ttgccagtg gttaaaagg cgccacagc attgaaaaa ttcccgatt gggattgta gacaccgat ggaccaaac ctcaaccga ctgtcaatt ttcaataac ttacctcgc tgtattagc cctcgttcg aaaaatttc cctaggtta aggcaaaca gtggatggg ttcaaccgc cggtcgaat ccttacaag accaggtcg gactcaccg gcccgcatt ttgcggaat ggaaaacag aggaaactg aaagatttg gaactgctg gaaggtctg gacattaca cacaagcgg cgaagacgg aactattcc gagttcagc ggcagcgaa atgtccaag ttttcaggc tacgagtcc gatagtagc ttctcggac ttgtcgtca gtacacggt gctgaaaat cccgaaacg aaggacatg gacggcatc aagaaggag aaaattgaa gacgatggc aatcctccc gccgtttcg gctgaaacc gaccacaat ttcaccgac accaactcg aatctttgt ctgaataag gacgtttcg gttaaggac gaacgagac gtcctggac agcatgcca tcggccccc agctccaaa gttcctgaa aaatcgata atcctcgac atcatgaag caagccttc aatgatccc ttcgaggaa gacaaacag aaaaagaag acttctgtc gaagacgtg aaaatcgaa ccaacaacc gaaaattcc gaagataaa aaccaagag gaagtggtc gaaaatggc gaaactgtg actgaagaa gtcgccaat ggcgaagtt atctcaaac ggcgacgca caaattgaa gaaaccgcc gaaaacctc gcgatcaaa gaaaacatc ctgcaagct ttaggcttg caaagtttg aaggcagct aaggaagcg cgactgaag agcaaggaa aaggccgaa cccaagagc gacctgtac accgggacg ctcaaaacc gtgatcaaa atcaaccgc ggcgagaag aagaaaggt aggaacatg aagatgact ttgcacaaa accaagaaa agcgagtgt gataacggc gacgaccac aaattaaaa gaagggccg tccacaagc tggaaacat ggtggtcag tcgtcggaa tcggccggt gcgcacaga aaatcacat tattctaac cgctctaac atggatggc agcagtgag cacacgtcc gatggcgag accggggcg caggaagga tcgaccaaa gctttggtc ataccggag aaagctagt tccttcagt atacacccg gggaggatc tgcaaggac gaatgttcc tattgctac ggcaagttc ggactgttc gatactccg tgtcatatc gcacaaatg aagagcgtc gaaaggcag aataaaatt ctagccgcc gaaaaacat ctaacgcgc gattcttgc ctttgtgac gcttgttat cgccacgtt gaccgtaag gccaacacg ccctcttac acgaacaaa tcttacaag agaagtgca accgttgca ccaggacct agacaggat cactgccac gtgttgggg tgtcgtaaa gtcgccaca aatatactg cggaggaag tggatcatt aaaatgagg aggagtata tgcgaagtg ataaacata gatttagat aatcctggt cttcattct attcctatt tgtgatgag cattactcg gcccttgaa tacctcatg gtgtgtgct atgtgtaaa agaagatta gctaagaat cacgttcat tatttggga cctgaaacc gtcgattta aacttagct ttgaccacg gaaggaatt cccttaagt ttaaccgat aaaccggtc gtttgcaaa ctgtgtaaa tatttcgct acgattatt ctcaaaccg cctgaagat cggcccgaa aacagctca acctttttt gttgaatac aagaagagg ctgcttcat ttcaacgat atcgtcccg atggatgaa gcggcggcc gaagagccg ataataatt cccacaaag gacagcaaa atcgaaaaa gagcaagtc cggaagaaa cggaagata tcaaagaac cagtcagtg cccggagac ggttcagat tctcaggta gaagtagaa aaatcggac caaagtcgt gtgccttca accgattca acaaaccaa tcacgtcca gaatctccc tctgactac atggtggac tacaacact ttaattcca tccatagca atggaatgt ggcagtgat agtgaatct aaaaaagaa caaccacca aaaccaacc catttgaag aaagtcgga cgtccagaa accatcaaa caagcagtg gaaatatca aagaacatg aaactgaaa aacgacaaa agtagtgat atcgcggtc caaagacta ggatcaaat ccttccata tcagtccga caattgttc ccttgcgag gaagatctt ccacttcag ggcaccata gaattcggc aacgtcaaa gaacggacg cccgaaggc tgggagaag tgcacctca gtcatacaa tacgacatg gaaaccaaa cacttatgg caagaattg caaaaacca tacggaaac caaagcagc ttcctgcga catttaatc ctcctggag aaatatttc cgcaacggt gatttaatc ctagcaccc aacgctagc caccattca atcaactac agcgaatcg gtccacaac cgcctccgc gcctacgac aacatccca tcaagcgct ggcaacgtc caacccatc agcatgatc ccattcaac aaagtccag aaaagtacc aacggaatc gtcaccacc gccaacaca aacccggcc aaagaaccc ccacctgca gtcccacca accataaac gcagcaaac gtccccaaa agcaccccc attacaata tcccagcta aacagcccc cctttaatc ccacctaac cccccaccc cccaaaccc aagcccgtg ggcgccccc ccaggcctc atctccctg caccctggc accaaccgg ccagtggcc cccctagtc aaagccccc caatcccag aaaatcaaa ttcccgata acgaaaaac tggcgaccg aatttaatc ccaatcgac ccttcgaag aaaaactcc gaacgcaaa gccggcttg gtgcaggtg atttcgggc gggaaaccc taccatatc actctagaa gactacaaa aaaatgtgt gctatcaag aagagtttc gagctgaag cagaagcga ctccaggag gcggcaaaa ggggcgcaa acgtcggtg ctaaaggcg gtaacgccg cgcaaaagc ttgatgatt tccaagggg aaggccgaa gagtcggag acgatttta gacaagttg gacaagcaa gtggaaaag ttagaatcg agactgaac gaaaataaa ccgccgtta tcacttccg aaaatacct aaatcgttg acggttatc ccgcagacg gtgactcgg aaaccctcc cggccttca agtcccgtt ctactaatt acccccaaa ccaaaacag atagcaaac aagtcatga ttaattctg acgtgtttt acaaagtgc ctcaaagaa atcgtcttt tgtacgcta gaagagaga ttgaatata tcgacagtt ttgtttttt attttatgc aaataaatt gttggcgga attgtaagt ttgctttga ttatattgt tacgagatt gacacgagt tagaggata tttttttat gtaacttgt aaatatccc aatccactc ttaaacgac gtatgtgtt cagtagctg catacatga tttcgtttg gtcaaaatt ccagcaata acggtcgtc tttccatta ccaatttat agtttcgag tacttagaa agaagggaa aatgtacaa acagtgcga gtgtttgca cgtttttcg gttccaatg ctcattaat catttaatt gtaatccta atttattac agttttagt atttttgta tacctatgt ttagttgga tgttattgg cttgtaatg atttgtgcc ggtcagatg atcggctag aatgtgaaa ttgaaaagt ctgaaatat tgttaattt ttctgaaac ctgtataaa gcccactat ttgaagtta ctagttaat tcattataa ttatttatg tacataaac cgggcgtta caataattg aaaataaat aatttaata catt
