Status
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Gene information
General information
TC032755
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007639,TC007637,TC007638
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1852232..1970797
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032755
    iBeetle screen iB_04358

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: bad quality of preparation,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    iBeetle screen iB_04357

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber potentially number increased - Number of animals showing the phenotype: 2

    Comment: No. of nucleus has increased in vitellogenic & previtellogenic egg chamber.  
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032755 RA
      MLSQRPRLP ALAALFPNV FIYYICTRR VYRSCPHQS 
      STECTPCAR RPARPSIRA LTVATPLLG DGLWCSALG 
      VRVAMDNSG MDSASEHSS HASSGSPAV AVKPSPSPP 
      AANSNTHQV HSPRQASPL GPPMSMAHP LSPPPVSSA 
      SLARSMALG HLPPPGLGL LNSLGVLHS PLDLMAHHA 
      PPRSYNSPP PISTSDPTA NECKLVDYR GQKVAAFII 
      AGDTMLCLP QAFELFLKH LVGGLHTVY TKLKRLDIV 
      PLVCNVEQV RILRGLGAI QPGVNRCKL LSCKDFDTL 
      YRDCTTASS RPGRPPKRA PVGLSLAAS HLQQQQLKK 
      QRLDNGDYP YENGHMGAE FPLDIMMRR DMSRLEKSP 
      LLANGYNHP PHLSHMQFM QLPHPAAAH SALLSPAMP 
      HNLTRHDGS VIKNQGMPT MEAIARSGI WENCRAAYE 
      DIVKHLERL REERCDPDR PLPLDQKAR DLSPRNGSP 
      TDHSPVLNL SKSGGGSAG SLGDGDHSG SEADGPDVP 
      PSPRSPRSA AEDEDDDNI SDPEDDDDK DQDMDEGDL 
      PLPAAPGGD SQQAALNYS TLASAVASA NGPSQDPSI 
      SSTETLLRN IQGLLKVAA DNARQQERQ INYEKGTNS 
      NMVYLLSLA QYYMYLAAT QKC
    • >TC032755 RB
      MLSQRPRLP ALAALFPNV FIYYICTRR VYRSCPHQS 
      STECTPCAR RPARPSIRA LTVATPLLG DGLWCSALG 
      VRVAMDNSG MDSASEHSS HASSGSPAV AVKPSPSPP 
      AANSNTHQV HSPRQASPL GPPMSMAHP LSPPPVSSA 
      SLARSMALG HLPPPGLGL LNSLGVLHS PLDLMAHHA 
      PPRSYNSPP PISTSDPTA NECKLVDYR GQKVAAFII 
      AGDTMLCLP QAFELFLKH LVGGLHTVY TKLKRLDIV 
      PLVCNVEQV RILRGLGAI QPGVNRCKL LSCKDFDTL 
      YRDCTTASS RPGRPPKRA PVGLSLAAS HLQQQQLKK 
      QRLDNGDYP YENGHMGDM SRLEKSPLL ANGYNHPPH 
      LSHMQFMQL PHPAAAHSA LLSPAMPHN LTRHDGSVI 
      KNQGMPTME AIARSGIWE NCRAAYEDI VKHLERLRE 
      ERCDPDRPL PLDQKARDL SPRNGSPTD HSPVLNLSK 
      SGGGSAGSL GDGDHSGSE ADGPDVPPS PRSPRSAAE 
      DEDDDNISD PEDDDDKDQ DMDEGDLPL PAAPGGDSQ 
      QAALNYSTL ASAVASANG PSQDPSISS TETLLRNIQ 
      GLLKVAADN ARQQERQIN YEKGTNSNM VYLLSLAQY 
      YMYLAATQK C
    • >TC032755 RC
      MLSQRPRLP ALAALFPNV FIYYICTRR VYRSCPHQS 
      STECTPCAR RPARPSIRA LTVATPLLG DGLWCSALG 
      VRVAMDNSG MDSASEHSS HASSGSPAV AVKPSPSPP 
      AANSNTHQV HSPRQASPL GPPMSMAHP LSPPPVSSA 
      SLARSMALG HLPPPGLGL LNSLGVLHS PLDLMAHHA 
      PPRSYNSPP PISTSDPTA NECKLVDYR GQKVAAFII 
      AGDTMLCLP QAFELFLKH LVGGLHTVY TKLKRLDIV 
      PLVCNVEQV RILRGLGAI QPGVNRCKL LSCKDFDTL 
      YRDCTTASS RPGRPPKRA PVGLSLAAS HLQQQQLKK 
      QRLDNGDYP YENGHMGDM SRLEKSPLL ANGYNHPPH 
      LSHMQFMQL PHPAAAHSA LLSPAMPHN LTRHDGSVI 
      KNQGMPTME AIARSGIWE NCRAAYEDI VKHLERLRE 
      ERCDPDRPL PLDQKARDL SPRNGSPTD HSPVLNLSK 
      SGGGSAGSL GDGDHSGSE ADGPDVPPS PRSPRSAAE 
      DEDDDNISD PEDDDDKDQ DMDEGDLPL PAAPGGDSQ 
      QAALNYSTL ASAVASANG PSQDPSISS TETLLRNIQ 
      GLLKVAADN ARQQERQIN YEKAELKMD VLREREVKD 
      SLERQLVDE QKMRGAAHP SVKRTQLEI RIMQTSCVQ 
      VWPAFLAPG RFATTRTTS AREARNKDL RGLIQIEPR 
      AGSCTPGEN PLDGKPDSR LKFIISSQT DASQLTKIF 
      AFSALVQKR LRRERRARR RLQDQLEAE VKRRTLMEE 
      ALKAAGAAE QLRIINEKV APSEQKLSN PKVTTSSTP 
      NQSISSERE RVSERVERM ERERVESPP PAYGQQTVR 
      EPPPPPPEN KPWGYSGID LMNTGAAFW QNYSESLAQ 
      ELELERKSR QQQVERDVK SPLQDRSGY YKNSMLFTS 
      TAT
    CDS sequence
    • >TC032755 RA
      atgctctcc caaaggccc cgccttccg gcacttgca 
      gcacttttt cccaatgtt tttatttat tacatttgt 
      acccggcga gtgtaccgg tcgtgtccg caccagtcg 
      agtacagag tgtaccccg tgcgcacga cgccccgcc 
      cggccatcc atccgagcc ctcactgtt gcgactcct 
      cttctgggt gatggactc tggtgcagt gctctaggt 
      gttcgcgtc gcgatggac aactccggg atggactcc 
      gccagcgag cacagcagc cacgcgtcc tcgggctcg 
      ccggccgtc gccgtcaag ccgtcgccg tcgccgccc 
      gccgccaac agcaacacg caccaggtg cacagcccg 
      cgccaggcc agcccgctg gggccgccc atgtccatg 
      gcgcacccg ctgagcccg ccgcccgtc agctcggcc 
      agtctggcc cgcagcatg gctctgggc cacctgccg 
      ccccccggc ttgggcttg ctcaactcg ctgggggtg 
      ctgcacagc cctctggac ctcatggcg catcacgca 
      ccgccacgg tcctacaac agcccgccg ccgatttcc 
      acctccgat cccacggct aatgagtgc aaactggtt 
      gattacaga ggacagaag gtcgccgct tttatcatc 
      gccggcgat accatgctg tgtcttccg caagccttt 
      gagctcttc ctcaagcac ctcgtcgga ggactgcat 
      acggtttat accaagctt aaaaggcta gacatcgtg 
      ccgttggtt tgtaacgtc gagcaggtc aggatcttg 
      agaggactc ggtgccatt caaccaggg gttaacaga 
      tgcaagcta ctctcatgc aaagacttc gatacactc 
      tacagagac tgcactacg gccagttcc aggcccgga 
      cgtcctccc aagcgagcg ccggtgggc ctcagcctc 
      gcagcatcg catcttcaa cagcaacag ctcaagaaa 
      caacgcctc gacaacggt gattatccg tacgaaaac 
      ggacacatg ggtgccgag tttccactt gacataatg 
      atgcgaaga gatatgtca agactagaa aaatcgccg 
      cttttggcg aacggttac aatcatcct cctcattta 
      agtcacatg cagtttatg caacttcct catccggcg 
      gctgcacat tcggcactc ttgtcgcct gcgatgccc 
      cacaatctc acaagacac gacggctcc gttataaag 
      aaccaagga atgcccacg atggaggct atagcgagg 
      tcaggcatt tgggaaaac tgcagggcc gcgtacgaa 
      gacatcgtc aaacattta gaaaggctg agggaggag 
      cggtgcgat cccgacaga ccgctgccc ttggaccag 
      aaagccagg gacctcagt ccacgaaac ggctctccg 
      accgatcac agtcccgtg ctgaatctg tcgaagagt 
      ggcggcggc agcgccggc tcgttaggg gacggcgac 
      cattcgggc agcgaagcc gacgggccg gacgtcccg 
      ccgtcgccg cgctccccc aggtccgcc gccgaggac 
      gaggacgac gacaacatc tccgacccg gaggacgac 
      gacgacaag gatcaagac atggacgag ggcgacctg 
      cccctcccg gcggccccc gggggcgac tcccagcag 
      gcagccctg aactattcg actcttgcg agtgcggtg 
      gcttcggcc aacggtcct agtcaagac ccgagcatt 
      tcctcgacg gagacgctg ctccgcaac atccaggga 
      ctcctgaag gtggcagcg gacaacgcc cgccagcag 
      gaacgccag atcaactac gagaaaggt acaaatagc 
      aacatggtg tatctgctg tcgctagcc cagtactat 
      atgtatcta gcagctacg caaaaatgc taa
    • >TC032755 RB
      atgctctcc caaaggccc cgccttccg gcacttgca 
      gcacttttt cccaatgtt tttatttat tacatttgt 
      acccggcga gtgtaccgg tcgtgtccg caccagtcg 
      agtacagag tgtaccccg tgcgcacga cgccccgcc 
      cggccatcc atccgagcc ctcactgtt gcgactcct 
      cttctgggt gatggactc tggtgcagt gctctaggt 
      gttcgcgtc gcgatggac aactccggg atggactcc 
      gccagcgag cacagcagc cacgcgtcc tcgggctcg 
      ccggccgtc gccgtcaag ccgtcgccg tcgccgccc 
      gccgccaac agcaacacg caccaggtg cacagcccg 
      cgccaggcc agcccgctg gggccgccc atgtccatg 
      gcgcacccg ctgagcccg ccgcccgtc agctcggcc 
      agtctggcc cgcagcatg gctctgggc cacctgccg 
      ccccccggc ttgggcttg ctcaactcg ctgggggtg 
      ctgcacagc cctctggac ctcatggcg catcacgca 
      ccgccacgg tcctacaac agcccgccg ccgatttcc 
      acctccgat cccacggct aatgagtgc aaactggtt 
      gattacaga ggacagaag gtcgccgct tttatcatc 
      gccggcgat accatgctg tgtcttccg caagccttt 
      gagctcttc ctcaagcac ctcgtcgga ggactgcat 
      acggtttat accaagctt aaaaggcta gacatcgtg 
      ccgttggtt tgtaacgtc gagcaggtc aggatcttg 
      agaggactc ggtgccatt caaccaggg gttaacaga 
      tgcaagcta ctctcatgc aaagacttc gatacactc 
      tacagagac tgcactacg gccagttcc aggcccgga 
      cgtcctccc aagcgagcg ccggtgggc ctcagcctc 
      gcagcatcg catcttcaa cagcaacag ctcaagaaa 
      caacgcctc gacaacggt gattatccg tacgaaaac 
      ggacacatg ggtgatatg tcaagacta gaaaaatcg 
      ccgcttttg gcgaacggt tacaatcat cctcctcat 
      ttaagtcac atgcagttt atgcaactt cctcatccg 
      gcggctgca cattcggca ctcttgtcg cctgcgatg 
      ccccacaat ctcacaaga cacgacggc tccgttata 
      aagaaccaa ggaatgccc acgatggag gctatagcg 
      aggtcaggc atttgggaa aactgcagg gccgcgtac 
      gaagacatc gtcaaacat ttagaaagg ctgagggag 
      gagcggtgc gatcccgac agaccgctg cccttggac 
      cagaaagcc agggacctc agtccacga aacggctct 
      ccgaccgat cacagtccc gtgctgaat ctgtcgaag 
      agtggcggc ggcagcgcc ggctcgtta ggggacggc 
      gaccattcg ggcagcgaa gccgacggg ccggacgtc 
      ccgccgtcg ccgcgctcc cccaggtcc gccgccgag 
      gacgaggac gacgacaac atctccgac ccggaggac 
      gacgacgac aaggatcaa gacatggac gagggcgac 
      ctgcccctc ccggcggcc cccgggggc gactcccag 
      caggcagcc ctgaactat tcgactctt gcgagtgcg 
      gtggcttcg gccaacggt cctagtcaa gacccgagc 
      atttcctcg acggagacg ctgctccgc aacatccag 
      ggactcctg aaggtggca gcggacaac gcccgccag 
      caggaacgc cagatcaac tacgagaaa ggtacaaat 
      agcaacatg gtgtatctg ctgtcgcta gcccagtac 
      tatatgtat ctagcagct acgcaaaaa tgctaa
    • >TC032755 RC
      atgctctcc caaaggccc cgccttccg gcacttgca 
      gcacttttt cccaatgtt tttatttat tacatttgt 
      acccggcga gtgtaccgg tcgtgtccg caccagtcg 
      agtacagag tgtaccccg tgcgcacga cgccccgcc 
      cggccatcc atccgagcc ctcactgtt gcgactcct 
      cttctgggt gatggactc tggtgcagt gctctaggt 
      gttcgcgtc gcgatggac aactccggg atggactcc 
      gccagcgag cacagcagc cacgcgtcc tcgggctcg 
      ccggccgtc gccgtcaag ccgtcgccg tcgccgccc 
      gccgccaac agcaacacg caccaggtg cacagcccg 
      cgccaggcc agcccgctg gggccgccc atgtccatg 
      gcgcacccg ctgagcccg ccgcccgtc agctcggcc 
      agtctggcc cgcagcatg gctctgggc cacctgccg 
      ccccccggc ttgggcttg ctcaactcg ctgggggtg 
      ctgcacagc cctctggac ctcatggcg catcacgca 
      ccgccacgg tcctacaac agcccgccg ccgatttcc 
      acctccgat cccacggct aatgagtgc aaactggtt 
      gattacaga ggacagaag gtcgccgct tttatcatc 
      gccggcgat accatgctg tgtcttccg caagccttt 
      gagctcttc ctcaagcac ctcgtcgga ggactgcat 
      acggtttat accaagctt aaaaggcta gacatcgtg 
      ccgttggtt tgtaacgtc gagcaggtc aggatcttg 
      agaggactc ggtgccatt caaccaggg gttaacaga 
      tgcaagcta ctctcatgc aaagacttc gatacactc 
      tacagagac tgcactacg gccagttcc aggcccgga 
      cgtcctccc aagcgagcg ccggtgggc ctcagcctc 
      gcagcatcg catcttcaa cagcaacag ctcaagaaa 
      caacgcctc gacaacggt gattatccg tacgaaaac 
      ggacacatg ggtgatatg tcaagacta gaaaaatcg 
      ccgcttttg gcgaacggt tacaatcat cctcctcat 
      ttaagtcac atgcagttt atgcaactt cctcatccg 
      gcggctgca cattcggca ctcttgtcg cctgcgatg 
      ccccacaat ctcacaaga cacgacggc tccgttata 
      aagaaccaa ggaatgccc acgatggag gctatagcg 
      aggtcaggc atttgggaa aactgcagg gccgcgtac 
      gaagacatc gtcaaacat ttagaaagg ctgagggag 
      gagcggtgc gatcccgac agaccgctg cccttggac 
      cagaaagcc agggacctc agtccacga aacggctct 
      ccgaccgat cacagtccc gtgctgaat ctgtcgaag 
      agtggcggc ggcagcgcc ggctcgtta ggggacggc 
      gaccattcg ggcagcgaa gccgacggg ccggacgtc 
      ccgccgtcg ccgcgctcc cccaggtcc gccgccgag 
      gacgaggac gacgacaac atctccgac ccggaggac 
      gacgacgac aaggatcaa gacatggac gagggcgac 
      ctgcccctc ccggcggcc cccgggggc gactcccag 
      caggcagcc ctgaactat tcgactctt gcgagtgcg 
      gtggcttcg gccaacggt cctagtcaa gacccgagc 
      atttcctcg acggagacg ctgctccgc aacatccag 
      ggactcctg aaggtggca gcggacaac gcccgccag 
      caggaacgc cagatcaac tacgagaaa gccgaattg 
      aagatggac gtgcttcgg gaaagagag gtgaaagac 
      agcttagaa agacaactg gtcgacgaa caaaaaatg 
      cgaggggcg gctcatcca agtgttaaa cggacacag 
      ctcgaaatc cgaatcatg caaacttcc tgtgtccag 
      gtctggcct gccttctta gcccccggc cgtttcgcg 
      acgacaagg acgaccagt gcgagggag gcgcgcaac 
      aaggacctc cgaggtctt attcaaata gagccgagg 
      gcagggtcg tgcactcct ggggagaac ccgctcgac 
      gggaagccc gactccaga ttgaaattc attattagc 
      tcgcaaact gatgcatca caattaacg aaaatattc 
      gccttttca gcactggtg cagaagcgg ctccggagg 
      gagcgtcgc gcacgtcgg cgactccaa gatcaactg 
      gaggcggaa gtcaaaaga cgcaccctg atggaagag 
      gcccttaag gcggcgggg gccgcagaa cagctcagg 
      ataattaat gaaaaagtg gcgccgagc gagcagaag 
      ctgagcaac ccgaaggtg accacaagc tcaacccca 
      aaccagagc atctcgtcg gagcgtgag agggtatcg 
      gaacgtgtc gaaaggatg gaaagagaa cgtgtggaa 
      agcccaccg ccggcatat gggcagcaa actgtccga 
      gagccgccg ccacctccg cccgaaaat aagccgtgg 
      ggctattcc ggaatcgac ctcatgaat accggagcc 
      gccttctgg cagaattac tccgaatcc ttagcacaa 
      gaactcgaa ttggagaga aaatcccgg cagcaacaa 
      gttgagaga gacgtgaag tcaccgctt caagatcgt 
      tccgggtat tacaaaaat tccatgctg ttcacaagc 
      acggctaca taa
    mRNA sequence
    • >TC032755 RA
      cacagaaat ccccataaa aatattata tgctctccc 
      aaaggcccc gccttccgg cacttgcag cactttttc 
      ccaatgttt ttatttatt acatttgta cccggcgag 
      tgtaccggt cgtgtccgc accagtcga gtacagagt 
      gtaccccgt gcgcacgac gccccgccc ggccatcca 
      tccgagccc tcactgttg cgactcctc ttctgggtg 
      atggactct ggtgcagtg ctctaggtg ttcgcgtcg 
      cgatggaca actccggga tggactccg ccagcgagc 
      acagcagcc acgcgtcct cgggctcgc cggccgtcg 
      ccgtcaagc cgtcgccgt cgccgcccg ccgccaaca 
      gcaacacgc accaggtgc acagcccgc gccaggcca 
      gcccgctgg ggccgccca tgtccatgg cgcacccgc 
      tgagcccgc cgcccgtca gctcggcca gtctggccc 
      gcagcatgg ctctgggcc acctgccgc cccccggct 
      tgggcttgc tcaactcgc tgggggtgc tgcacagcc 
      ctctggacc tcatggcgc atcacgcac cgccacggt 
      cctacaaca gcccgccgc cgatttcca cctccgatc 
      ccacggcta atgagtgca aactggttg attacagag 
      gacagaagg tcgccgctt ttatcatcg ccggcgata 
      ccatgctgt gtcttccgc aagcctttg agctcttcc 
      tcaagcacc tcgtcggag gactgcata cggtttata 
      ccaagctta aaaggctag acatcgtgc cgttggttt 
      gtaacgtcg agcaggtca ggatcttga gaggactcg 
      gtgccattc aaccagggg ttaacagat gcaagctac 
      tctcatgca aagacttcg atacactct acagagact 
      gcactacgg ccagttcca ggcccggac gtcctccca 
      agcgagcgc cggtgggcc tcagcctcg cagcatcgc 
      atcttcaac agcaacagc tcaagaaac aacgcctcg 
      acaacggtg attatccgt acgaaaacg gacacatgg 
      gtgccgagt ttccacttg acataatga tgcgaagag 
      atatgtcaa gactagaaa aatcgccgc ttttggcga 
      acggttaca atcatcctc ctcatttaa gtcacatgc 
      agtttatgc aacttcctc atccggcgg ctgcacatt 
      cggcactct tgtcgcctg cgatgcccc acaatctca 
      caagacacg acggctccg ttataaaga accaaggaa 
      tgcccacga tggaggcta tagcgaggt caggcattt 
      gggaaaact gcagggccg cgtacgaag acatcgtca 
      aacatttag aaaggctga gggaggagc ggtgcgatc 
      ccgacagac cgctgccct tggaccaga aagccaggg 
      acctcagtc cacgaaacg gctctccga ccgatcaca 
      gtcccgtgc tgaatctgt cgaagagtg gcggcggca 
      gcgccggct cgttagggg acggcgacc attcgggca 
      gcgaagccg acgggccgg acgtcccgc cgtcgccgc 
      gctccccca ggtccgccg ccgaggacg aggacgacg 
      acaacatct ccgacccgg aggacgacg acgacaagg 
      atcaagaca tggacgagg gcgacctgc ccctcccgg 
      cggcccccg ggggcgact cccagcagg cagccctga 
      actattcga ctcttgcga gtgcggtgg cttcggcca 
      acggtccta gtcaagacc cgagcattt cctcgacgg 
      agacgctgc tccgcaaca tccagggac tcctgaagg 
      tggcagcgg acaacgccc gccagcagg aacgccaga 
      tcaactacg agaaaggta caaatagca acatggtgt 
      atctgctgt cgctagccc agtactata tgtatctag 
      cagctacgc aaaaatgct aaattctga tgattgagt 
      gactcggtg taatatttt gtaaataca taaagtgcc 
      gctggaaaa tcggaaatt taaattgtg ggcgaaaat 
      tgcgtgatc tgagctggt agagtagtg aatttaagt 
      ttcgaattt tccaacgta cgattgcat agcaagtta 
      gtattgagt ttgtataaa tgacggcac tcgagtgca 
      catatcgac ttgtactct aaaaataag tagctttac 
      taggtgtac atacttaag cgtaatctg tggatagaa 
      gcagatatt ttctagcaa aggtaatgt tagagtgac 
      gactgttga gagtactaa aggacgcct cagataaga 
      atctgtgtg tgtttgaac ctgacccgg tgtttcagt 
      gatggaatt tggttattc tgtcggctg gttgttagt 
      atattatta gtaatgtgc cattacttc cagtgttgt 
      tagagtaga tgtttattt aaaaattag ttaatttaa 
      cttaaatat ttatatatt taaaataat ccgaaaatt 
      ttgatatat tctcctcct gtatattta cccggaata 
      taacaaaat tttccctgc accaaaaaa attcagtga 
      cgacttcct tccaacgga actcgatct gacgtctag 
      cattaggtt tatgtgtga tgtacatgt taacaattc 
      aaaattgaa aataaacga aattactat at
    • >TC032755 RB
      cacagaaat ccccataaa aatattata tgctctccc 
      aaaggcccc gccttccgg cacttgcag cactttttc 
      ccaatgttt ttatttatt acatttgta cccggcgag 
      tgtaccggt cgtgtccgc accagtcga gtacagagt 
      gtaccccgt gcgcacgac gccccgccc ggccatcca 
      tccgagccc tcactgttg cgactcctc ttctgggtg 
      atggactct ggtgcagtg ctctaggtg ttcgcgtcg 
      cgatggaca actccggga tggactccg ccagcgagc 
      acagcagcc acgcgtcct cgggctcgc cggccgtcg 
      ccgtcaagc cgtcgccgt cgccgcccg ccgccaaca 
      gcaacacgc accaggtgc acagcccgc gccaggcca 
      gcccgctgg ggccgccca tgtccatgg cgcacccgc 
      tgagcccgc cgcccgtca gctcggcca gtctggccc 
      gcagcatgg ctctgggcc acctgccgc cccccggct 
      tgggcttgc tcaactcgc tgggggtgc tgcacagcc 
      ctctggacc tcatggcgc atcacgcac cgccacggt 
      cctacaaca gcccgccgc cgatttcca cctccgatc 
      ccacggcta atgagtgca aactggttg attacagag 
      gacagaagg tcgccgctt ttatcatcg ccggcgata 
      ccatgctgt gtcttccgc aagcctttg agctcttcc 
      tcaagcacc tcgtcggag gactgcata cggtttata 
      ccaagctta aaaggctag acatcgtgc cgttggttt 
      gtaacgtcg agcaggtca ggatcttga gaggactcg 
      gtgccattc aaccagggg ttaacagat gcaagctac 
      tctcatgca aagacttcg atacactct acagagact 
      gcactacgg ccagttcca ggcccggac gtcctccca 
      agcgagcgc cggtgggcc tcagcctcg cagcatcgc 
      atcttcaac agcaacagc tcaagaaac aacgcctcg 
      acaacggtg attatccgt acgaaaacg gacacatgg 
      gtgatatgt caagactag aaaaatcgc cgcttttgg 
      cgaacggtt acaatcatc ctcctcatt taagtcaca 
      tgcagttta tgcaacttc ctcatccgg cggctgcac 
      attcggcac tcttgtcgc ctgcgatgc cccacaatc 
      tcacaagac acgacggct ccgttataa agaaccaag 
      gaatgccca cgatggagg ctatagcga ggtcaggca 
      tttgggaaa actgcaggg ccgcgtacg aagacatcg 
      tcaaacatt tagaaaggc tgagggagg agcggtgcg 
      atcccgaca gaccgctgc ccttggacc agaaagcca 
      gggacctca gtccacgaa acggctctc cgaccgatc 
      acagtcccg tgctgaatc tgtcgaaga gtggcggcg 
      gcagcgccg gctcgttag gggacggcg accattcgg 
      gcagcgaag ccgacgggc cggacgtcc cgccgtcgc 
      cgcgctccc ccaggtccg ccgccgagg acgaggacg 
      acgacaaca tctccgacc cggaggacg acgacgaca 
      aggatcaag acatggacg agggcgacc tgcccctcc 
      cggcggccc ccgggggcg actcccagc aggcagccc 
      tgaactatt cgactcttg cgagtgcgg tggcttcgg 
      ccaacggtc ctagtcaag acccgagca tttcctcga 
      cggagacgc tgctccgca acatccagg gactcctga 
      aggtggcag cggacaacg cccgccagc aggaacgcc 
      agatcaact acgagaaag gtacaaata gcaacatgg 
      tgtatctgc tgtcgctag cccagtact atatgtatc 
      tagcagcta cgcaaaaat gctaaattc tgatgattg 
      agtgactcg gtgtaatat tttgtaaat acataaagt 
      gccgctgga aaatcggaa atttaaatt gtgggcgaa 
      aattgcgtg atctgagct ggtagagta gtgaattta 
      agtttcgaa ttttccaac gtacgattg catagcaag 
      ttagtattg agtttgtat aaatgacgg cactcgagt 
      gcacatatc gacttgtac tctaaaaat aagtagctt 
      tactaggtg tacatactt aagcgtaat ctgtggata 
      gaagcagat attttctag caaaggtaa tgttagagt 
      gacgactgt tgagagtac taaaggacg cctcagata 
      agaatctgt gtgtgtttg aacctgacc cggtgtttc 
      agtgatgga atttggtta ttctgtcgg ctggttgtt 
      agtatatta ttagtaatg tgccattac ttccagtgt 
      tgttagagt agatgttta tttaaaaat tagttaatt 
      taacttaaa tatttatat atttaaaat aatccgaaa 
      attttgata tattctcct cctgtatat ttacccgga 
      atataacaa aattttccc tgcaccaaa aaaattcag 
      tgacgactt ccttccaac ggaactcga tctgacgtc 
      tagcattag gtttatgtg tgatgtaca tgttaacaa 
      ttcaaaatt gaaaataaa cgaaattac tatat
    • >TC032755 RC
      cacagaaat ccccataaa aatattata tgctctccc 
      aaaggcccc gccttccgg cacttgcag cactttttc 
      ccaatgttt ttatttatt acatttgta cccggcgag 
      tgtaccggt cgtgtccgc accagtcga gtacagagt 
      gtaccccgt gcgcacgac gccccgccc ggccatcca 
      tccgagccc tcactgttg cgactcctc ttctgggtg 
      atggactct ggtgcagtg ctctaggtg ttcgcgtcg 
      cgatggaca actccggga tggactccg ccagcgagc 
      acagcagcc acgcgtcct cgggctcgc cggccgtcg 
      ccgtcaagc cgtcgccgt cgccgcccg ccgccaaca 
      gcaacacgc accaggtgc acagcccgc gccaggcca 
      gcccgctgg ggccgccca tgtccatgg cgcacccgc 
      tgagcccgc cgcccgtca gctcggcca gtctggccc 
      gcagcatgg ctctgggcc acctgccgc cccccggct 
      tgggcttgc tcaactcgc tgggggtgc tgcacagcc 
      ctctggacc tcatggcgc atcacgcac cgccacggt 
      cctacaaca gcccgccgc cgatttcca cctccgatc 
      ccacggcta atgagtgca aactggttg attacagag 
      gacagaagg tcgccgctt ttatcatcg ccggcgata 
      ccatgctgt gtcttccgc aagcctttg agctcttcc 
      tcaagcacc tcgtcggag gactgcata cggtttata 
      ccaagctta aaaggctag acatcgtgc cgttggttt 
      gtaacgtcg agcaggtca ggatcttga gaggactcg 
      gtgccattc aaccagggg ttaacagat gcaagctac 
      tctcatgca aagacttcg atacactct acagagact 
      gcactacgg ccagttcca ggcccggac gtcctccca 
      agcgagcgc cggtgggcc tcagcctcg cagcatcgc 
      atcttcaac agcaacagc tcaagaaac aacgcctcg 
      acaacggtg attatccgt acgaaaacg gacacatgg 
      gtgatatgt caagactag aaaaatcgc cgcttttgg 
      cgaacggtt acaatcatc ctcctcatt taagtcaca 
      tgcagttta tgcaacttc ctcatccgg cggctgcac 
      attcggcac tcttgtcgc ctgcgatgc cccacaatc 
      tcacaagac acgacggct ccgttataa agaaccaag 
      gaatgccca cgatggagg ctatagcga ggtcaggca 
      tttgggaaa actgcaggg ccgcgtacg aagacatcg 
      tcaaacatt tagaaaggc tgagggagg agcggtgcg 
      atcccgaca gaccgctgc ccttggacc agaaagcca 
      gggacctca gtccacgaa acggctctc cgaccgatc 
      acagtcccg tgctgaatc tgtcgaaga gtggcggcg 
      gcagcgccg gctcgttag gggacggcg accattcgg 
      gcagcgaag ccgacgggc cggacgtcc cgccgtcgc 
      cgcgctccc ccaggtccg ccgccgagg acgaggacg 
      acgacaaca tctccgacc cggaggacg acgacgaca 
      aggatcaag acatggacg agggcgacc tgcccctcc 
      cggcggccc ccgggggcg actcccagc aggcagccc 
      tgaactatt cgactcttg cgagtgcgg tggcttcgg 
      ccaacggtc ctagtcaag acccgagca tttcctcga 
      cggagacgc tgctccgca acatccagg gactcctga 
      aggtggcag cggacaacg cccgccagc aggaacgcc 
      agatcaact acgagaaag ccgaattga agatggacg 
      tgcttcggg aaagagagg tgaaagaca gcttagaaa 
      gacaactgg tcgacgaac aaaaaatgc gaggggcgg 
      ctcatccaa gtgttaaac ggacacagc tcgaaatcc 
      gaatcatgc aaacttcct gtgtccagg tctggcctg 
      ccttcttag cccccggcc gtttcgcga cgacaagga 
      cgaccagtg cgagggagg cgcgcaaca aggacctcc 
      gaggtctta ttcaaatag agccgaggg cagggtcgt 
      gcactcctg gggagaacc cgctcgacg ggaagcccg 
      actccagat tgaaattca ttattagct cgcaaactg 
      atgcatcac aattaacga aaatattcg ccttttcag 
      cactggtgc agaagcggc tccggaggg agcgtcgcg 
      cacgtcggc gactccaag atcaactgg aggcggaag 
      tcaaaagac gcaccctga tggaagagg cccttaagg 
      cggcggggg ccgcagaac agctcagga taattaatg 
      aaaaagtgg cgccgagcg agcagaagc tgagcaacc 
      cgaaggtga ccacaagct caaccccaa accagagca 
      tctcgtcgg agcgtgaga gggtatcgg aacgtgtcg 
      aaaggatgg aaagagaac gtgtggaaa gcccaccgc 
      cggcatatg ggcagcaaa ctgtccgag agccgccgc 
      cacctccgc ccgaaaata agccgtggg gctattccg 
      gaatcgacc tcatgaata ccggagccg ccttctggc 
      agaattact ccgaatcct tagcacaag aactcgaat 
      tggagagaa aatcccggc agcaacaag ttgagagag 
      acgtgaagt caccgcttc aagatcgtt ccgggtatt 
      acaaaaatt ccatgctgt tcacaagca cggctacat 
      aaacataac gtacgagtt ttgcttaga taaaagtat 
      aaagtaggt tttagatca gagcgtaca gtgcttagc 
      aatgtttcg attggtatt atgcattcg ttttaaacg 
      agatattaa gttttttcc ttaaaatac gtccagtgt 
      cgtggagca tatcagtta gtgattgtt tgattttta 
      agactgatt ttgggcatg gagaggaac gataacaat 
      ttcagtact taacttttc ttattgctc agaattcgc 
      acttcgtgt acataaaca cacctgtat ataatttct 
      attttgtaa tccaaccca attacttta ctatgactg 
      tttggaatt attctaatt aaatatctc cgctttaca 
      tgtacatat gagcgtcag tataaacct gtagaaaag 
      tcatttttc atgtaacaa cgcagaata gaagattgt 
      atattaaaa tttttataa agttaaagt tttgcatat 
      ccatgtgaa ctttctacg atatgatat gaacataaa 
      attcatttg tacattatt tcgtaatct gaaatgttt 
      tacacagtg taggatttt ctgacgaaa agtatacgc 
      cattttgac aatcattgt cagaaattt ttcaaatat 
      caatattgt cgtttgttt acactcagt attattcag 
      attaggtgc aatactgaa ccacaaatt gtacgaacg 
      gtagtgttc ttaatatag cacagtttt tttcttact 
      tgcttgtat cataagtag gctagttta taaactgtt 
      cgtgttaga ctatgtaag aggcagtgt gtatgtatt 
      tattgtaaa taaatgtat gtgtgtgcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY