| General information | |||
| TC032755
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 1852232..1970797 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032755 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04358 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04357 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
| Comment: No. of nucleus has increased in vitellogenic & previtellogenic egg chamber. |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032755 RA
MLSQRPRLP ALAALFPNV FIYYICTRR VYRSCPHQS STECTPCAR RPARPSIRA LTVATPLLG DGLWCSALG VRVAMDNSG MDSASEHSS HASSGSPAV AVKPSPSPP AANSNTHQV HSPRQASPL GPPMSMAHP LSPPPVSSA SLARSMALG HLPPPGLGL LNSLGVLHS PLDLMAHHA PPRSYNSPP PISTSDPTA NECKLVDYR GQKVAAFII AGDTMLCLP QAFELFLKH LVGGLHTVY TKLKRLDIV PLVCNVEQV RILRGLGAI QPGVNRCKL LSCKDFDTL YRDCTTASS RPGRPPKRA PVGLSLAAS HLQQQQLKK QRLDNGDYP YENGHMGAE FPLDIMMRR DMSRLEKSP LLANGYNHP PHLSHMQFM QLPHPAAAH SALLSPAMP HNLTRHDGS VIKNQGMPT MEAIARSGI WENCRAAYE DIVKHLERL REERCDPDR PLPLDQKAR DLSPRNGSP TDHSPVLNL SKSGGGSAG SLGDGDHSG SEADGPDVP PSPRSPRSA AEDEDDDNI SDPEDDDDK DQDMDEGDL PLPAAPGGD SQQAALNYS TLASAVASA NGPSQDPSI SSTETLLRN IQGLLKVAA DNARQQERQ INYEKGTNS NMVYLLSLA QYYMYLAAT QKC
- >TC032755 RB
MLSQRPRLP ALAALFPNV FIYYICTRR VYRSCPHQS STECTPCAR RPARPSIRA LTVATPLLG DGLWCSALG VRVAMDNSG MDSASEHSS HASSGSPAV AVKPSPSPP AANSNTHQV HSPRQASPL GPPMSMAHP LSPPPVSSA SLARSMALG HLPPPGLGL LNSLGVLHS PLDLMAHHA PPRSYNSPP PISTSDPTA NECKLVDYR GQKVAAFII AGDTMLCLP QAFELFLKH LVGGLHTVY TKLKRLDIV PLVCNVEQV RILRGLGAI QPGVNRCKL LSCKDFDTL YRDCTTASS RPGRPPKRA PVGLSLAAS HLQQQQLKK QRLDNGDYP YENGHMGDM SRLEKSPLL ANGYNHPPH LSHMQFMQL PHPAAAHSA LLSPAMPHN LTRHDGSVI KNQGMPTME AIARSGIWE NCRAAYEDI VKHLERLRE ERCDPDRPL PLDQKARDL SPRNGSPTD HSPVLNLSK SGGGSAGSL GDGDHSGSE ADGPDVPPS PRSPRSAAE DEDDDNISD PEDDDDKDQ DMDEGDLPL PAAPGGDSQ QAALNYSTL ASAVASANG PSQDPSISS TETLLRNIQ GLLKVAADN ARQQERQIN YEKGTNSNM VYLLSLAQY YMYLAATQK C
- >TC032755 RC
MLSQRPRLP ALAALFPNV FIYYICTRR VYRSCPHQS STECTPCAR RPARPSIRA LTVATPLLG DGLWCSALG VRVAMDNSG MDSASEHSS HASSGSPAV AVKPSPSPP AANSNTHQV HSPRQASPL GPPMSMAHP LSPPPVSSA SLARSMALG HLPPPGLGL LNSLGVLHS PLDLMAHHA PPRSYNSPP PISTSDPTA NECKLVDYR GQKVAAFII AGDTMLCLP QAFELFLKH LVGGLHTVY TKLKRLDIV PLVCNVEQV RILRGLGAI QPGVNRCKL LSCKDFDTL YRDCTTASS RPGRPPKRA PVGLSLAAS HLQQQQLKK QRLDNGDYP YENGHMGDM SRLEKSPLL ANGYNHPPH LSHMQFMQL PHPAAAHSA LLSPAMPHN LTRHDGSVI KNQGMPTME AIARSGIWE NCRAAYEDI VKHLERLRE ERCDPDRPL PLDQKARDL SPRNGSPTD HSPVLNLSK SGGGSAGSL GDGDHSGSE ADGPDVPPS PRSPRSAAE DEDDDNISD PEDDDDKDQ DMDEGDLPL PAAPGGDSQ QAALNYSTL ASAVASANG PSQDPSISS TETLLRNIQ GLLKVAADN ARQQERQIN YEKAELKMD VLREREVKD SLERQLVDE QKMRGAAHP SVKRTQLEI RIMQTSCVQ VWPAFLAPG RFATTRTTS AREARNKDL RGLIQIEPR AGSCTPGEN PLDGKPDSR LKFIISSQT DASQLTKIF AFSALVQKR LRRERRARR RLQDQLEAE VKRRTLMEE ALKAAGAAE QLRIINEKV APSEQKLSN PKVTTSSTP NQSISSERE RVSERVERM ERERVESPP PAYGQQTVR EPPPPPPEN KPWGYSGID LMNTGAAFW QNYSESLAQ ELELERKSR QQQVERDVK SPLQDRSGY YKNSMLFTS TAT
- >TC032755 RA
atgctctcc caaaggccc cgccttccg gcacttgca gcacttttt cccaatgtt tttatttat tacatttgt acccggcga gtgtaccgg tcgtgtccg caccagtcg agtacagag tgtaccccg tgcgcacga cgccccgcc cggccatcc atccgagcc ctcactgtt gcgactcct cttctgggt gatggactc tggtgcagt gctctaggt gttcgcgtc gcgatggac aactccggg atggactcc gccagcgag cacagcagc cacgcgtcc tcgggctcg ccggccgtc gccgtcaag ccgtcgccg tcgccgccc gccgccaac agcaacacg caccaggtg cacagcccg cgccaggcc agcccgctg gggccgccc atgtccatg gcgcacccg ctgagcccg ccgcccgtc agctcggcc agtctggcc cgcagcatg gctctgggc cacctgccg ccccccggc ttgggcttg ctcaactcg ctgggggtg ctgcacagc cctctggac ctcatggcg catcacgca ccgccacgg tcctacaac agcccgccg ccgatttcc acctccgat cccacggct aatgagtgc aaactggtt gattacaga ggacagaag gtcgccgct tttatcatc gccggcgat accatgctg tgtcttccg caagccttt gagctcttc ctcaagcac ctcgtcgga ggactgcat acggtttat accaagctt aaaaggcta gacatcgtg ccgttggtt tgtaacgtc gagcaggtc aggatcttg agaggactc ggtgccatt caaccaggg gttaacaga tgcaagcta ctctcatgc aaagacttc gatacactc tacagagac tgcactacg gccagttcc aggcccgga cgtcctccc aagcgagcg ccggtgggc ctcagcctc gcagcatcg catcttcaa cagcaacag ctcaagaaa caacgcctc gacaacggt gattatccg tacgaaaac ggacacatg ggtgccgag tttccactt gacataatg atgcgaaga gatatgtca agactagaa aaatcgccg cttttggcg aacggttac aatcatcct cctcattta agtcacatg cagtttatg caacttcct catccggcg gctgcacat tcggcactc ttgtcgcct gcgatgccc cacaatctc acaagacac gacggctcc gttataaag aaccaagga atgcccacg atggaggct atagcgagg tcaggcatt tgggaaaac tgcagggcc gcgtacgaa gacatcgtc aaacattta gaaaggctg agggaggag cggtgcgat cccgacaga ccgctgccc ttggaccag aaagccagg gacctcagt ccacgaaac ggctctccg accgatcac agtcccgtg ctgaatctg tcgaagagt ggcggcggc agcgccggc tcgttaggg gacggcgac cattcgggc agcgaagcc gacgggccg gacgtcccg ccgtcgccg cgctccccc aggtccgcc gccgaggac gaggacgac gacaacatc tccgacccg gaggacgac gacgacaag gatcaagac atggacgag ggcgacctg cccctcccg gcggccccc gggggcgac tcccagcag gcagccctg aactattcg actcttgcg agtgcggtg gcttcggcc aacggtcct agtcaagac ccgagcatt tcctcgacg gagacgctg ctccgcaac atccaggga ctcctgaag gtggcagcg gacaacgcc cgccagcag gaacgccag atcaactac gagaaaggt acaaatagc aacatggtg tatctgctg tcgctagcc cagtactat atgtatcta gcagctacg caaaaatgc taa
- >TC032755 RB
atgctctcc caaaggccc cgccttccg gcacttgca gcacttttt cccaatgtt tttatttat tacatttgt acccggcga gtgtaccgg tcgtgtccg caccagtcg agtacagag tgtaccccg tgcgcacga cgccccgcc cggccatcc atccgagcc ctcactgtt gcgactcct cttctgggt gatggactc tggtgcagt gctctaggt gttcgcgtc gcgatggac aactccggg atggactcc gccagcgag cacagcagc cacgcgtcc tcgggctcg ccggccgtc gccgtcaag ccgtcgccg tcgccgccc gccgccaac agcaacacg caccaggtg cacagcccg cgccaggcc agcccgctg gggccgccc atgtccatg gcgcacccg ctgagcccg ccgcccgtc agctcggcc agtctggcc cgcagcatg gctctgggc cacctgccg ccccccggc ttgggcttg ctcaactcg ctgggggtg ctgcacagc cctctggac ctcatggcg catcacgca ccgccacgg tcctacaac agcccgccg ccgatttcc acctccgat cccacggct aatgagtgc aaactggtt gattacaga ggacagaag gtcgccgct tttatcatc gccggcgat accatgctg tgtcttccg caagccttt gagctcttc ctcaagcac ctcgtcgga ggactgcat acggtttat accaagctt aaaaggcta gacatcgtg ccgttggtt tgtaacgtc gagcaggtc aggatcttg agaggactc ggtgccatt caaccaggg gttaacaga tgcaagcta ctctcatgc aaagacttc gatacactc tacagagac tgcactacg gccagttcc aggcccgga cgtcctccc aagcgagcg ccggtgggc ctcagcctc gcagcatcg catcttcaa cagcaacag ctcaagaaa caacgcctc gacaacggt gattatccg tacgaaaac ggacacatg ggtgatatg tcaagacta gaaaaatcg ccgcttttg gcgaacggt tacaatcat cctcctcat ttaagtcac atgcagttt atgcaactt cctcatccg gcggctgca cattcggca ctcttgtcg cctgcgatg ccccacaat ctcacaaga cacgacggc tccgttata aagaaccaa ggaatgccc acgatggag gctatagcg aggtcaggc atttgggaa aactgcagg gccgcgtac gaagacatc gtcaaacat ttagaaagg ctgagggag gagcggtgc gatcccgac agaccgctg cccttggac cagaaagcc agggacctc agtccacga aacggctct ccgaccgat cacagtccc gtgctgaat ctgtcgaag agtggcggc ggcagcgcc ggctcgtta ggggacggc gaccattcg ggcagcgaa gccgacggg ccggacgtc ccgccgtcg ccgcgctcc cccaggtcc gccgccgag gacgaggac gacgacaac atctccgac ccggaggac gacgacgac aaggatcaa gacatggac gagggcgac ctgcccctc ccggcggcc cccgggggc gactcccag caggcagcc ctgaactat tcgactctt gcgagtgcg gtggcttcg gccaacggt cctagtcaa gacccgagc atttcctcg acggagacg ctgctccgc aacatccag ggactcctg aaggtggca gcggacaac gcccgccag caggaacgc cagatcaac tacgagaaa ggtacaaat agcaacatg gtgtatctg ctgtcgcta gcccagtac tatatgtat ctagcagct acgcaaaaa tgctaa
- >TC032755 RC
atgctctcc caaaggccc cgccttccg gcacttgca gcacttttt cccaatgtt tttatttat tacatttgt acccggcga gtgtaccgg tcgtgtccg caccagtcg agtacagag tgtaccccg tgcgcacga cgccccgcc cggccatcc atccgagcc ctcactgtt gcgactcct cttctgggt gatggactc tggtgcagt gctctaggt gttcgcgtc gcgatggac aactccggg atggactcc gccagcgag cacagcagc cacgcgtcc tcgggctcg ccggccgtc gccgtcaag ccgtcgccg tcgccgccc gccgccaac agcaacacg caccaggtg cacagcccg cgccaggcc agcccgctg gggccgccc atgtccatg gcgcacccg ctgagcccg ccgcccgtc agctcggcc agtctggcc cgcagcatg gctctgggc cacctgccg ccccccggc ttgggcttg ctcaactcg ctgggggtg ctgcacagc cctctggac ctcatggcg catcacgca ccgccacgg tcctacaac agcccgccg ccgatttcc acctccgat cccacggct aatgagtgc aaactggtt gattacaga ggacagaag gtcgccgct tttatcatc gccggcgat accatgctg tgtcttccg caagccttt gagctcttc ctcaagcac ctcgtcgga ggactgcat acggtttat accaagctt aaaaggcta gacatcgtg ccgttggtt tgtaacgtc gagcaggtc aggatcttg agaggactc ggtgccatt caaccaggg gttaacaga tgcaagcta ctctcatgc aaagacttc gatacactc tacagagac tgcactacg gccagttcc aggcccgga cgtcctccc aagcgagcg ccggtgggc ctcagcctc gcagcatcg catcttcaa cagcaacag ctcaagaaa caacgcctc gacaacggt gattatccg tacgaaaac ggacacatg ggtgatatg tcaagacta gaaaaatcg ccgcttttg gcgaacggt tacaatcat cctcctcat ttaagtcac atgcagttt atgcaactt cctcatccg gcggctgca cattcggca ctcttgtcg cctgcgatg ccccacaat ctcacaaga cacgacggc tccgttata aagaaccaa ggaatgccc acgatggag gctatagcg aggtcaggc atttgggaa aactgcagg gccgcgtac gaagacatc gtcaaacat ttagaaagg ctgagggag gagcggtgc gatcccgac agaccgctg cccttggac cagaaagcc agggacctc agtccacga aacggctct ccgaccgat cacagtccc gtgctgaat ctgtcgaag agtggcggc ggcagcgcc ggctcgtta ggggacggc gaccattcg ggcagcgaa gccgacggg ccggacgtc ccgccgtcg ccgcgctcc cccaggtcc gccgccgag gacgaggac gacgacaac atctccgac ccggaggac gacgacgac aaggatcaa gacatggac gagggcgac ctgcccctc ccggcggcc cccgggggc gactcccag caggcagcc ctgaactat tcgactctt gcgagtgcg gtggcttcg gccaacggt cctagtcaa gacccgagc atttcctcg acggagacg ctgctccgc aacatccag ggactcctg aaggtggca gcggacaac gcccgccag caggaacgc cagatcaac tacgagaaa gccgaattg aagatggac gtgcttcgg gaaagagag gtgaaagac agcttagaa agacaactg gtcgacgaa caaaaaatg cgaggggcg gctcatcca agtgttaaa cggacacag ctcgaaatc cgaatcatg caaacttcc tgtgtccag gtctggcct gccttctta gcccccggc cgtttcgcg acgacaagg acgaccagt gcgagggag gcgcgcaac aaggacctc cgaggtctt attcaaata gagccgagg gcagggtcg tgcactcct ggggagaac ccgctcgac gggaagccc gactccaga ttgaaattc attattagc tcgcaaact gatgcatca caattaacg aaaatattc gccttttca gcactggtg cagaagcgg ctccggagg gagcgtcgc gcacgtcgg cgactccaa gatcaactg gaggcggaa gtcaaaaga cgcaccctg atggaagag gcccttaag gcggcgggg gccgcagaa cagctcagg ataattaat gaaaaagtg gcgccgagc gagcagaag ctgagcaac ccgaaggtg accacaagc tcaacccca aaccagagc atctcgtcg gagcgtgag agggtatcg gaacgtgtc gaaaggatg gaaagagaa cgtgtggaa agcccaccg ccggcatat gggcagcaa actgtccga gagccgccg ccacctccg cccgaaaat aagccgtgg ggctattcc ggaatcgac ctcatgaat accggagcc gccttctgg cagaattac tccgaatcc ttagcacaa gaactcgaa ttggagaga aaatcccgg cagcaacaa gttgagaga gacgtgaag tcaccgctt caagatcgt tccgggtat tacaaaaat tccatgctg ttcacaagc acggctaca taa
- >TC032755 RA
cacagaaat ccccataaa aatattata tgctctccc aaaggcccc gccttccgg cacttgcag cactttttc ccaatgttt ttatttatt acatttgta cccggcgag tgtaccggt cgtgtccgc accagtcga gtacagagt gtaccccgt gcgcacgac gccccgccc ggccatcca tccgagccc tcactgttg cgactcctc ttctgggtg atggactct ggtgcagtg ctctaggtg ttcgcgtcg cgatggaca actccggga tggactccg ccagcgagc acagcagcc acgcgtcct cgggctcgc cggccgtcg ccgtcaagc cgtcgccgt cgccgcccg ccgccaaca gcaacacgc accaggtgc acagcccgc gccaggcca gcccgctgg ggccgccca tgtccatgg cgcacccgc tgagcccgc cgcccgtca gctcggcca gtctggccc gcagcatgg ctctgggcc acctgccgc cccccggct tgggcttgc tcaactcgc tgggggtgc tgcacagcc ctctggacc tcatggcgc atcacgcac cgccacggt cctacaaca gcccgccgc cgatttcca cctccgatc ccacggcta atgagtgca aactggttg attacagag gacagaagg tcgccgctt ttatcatcg ccggcgata ccatgctgt gtcttccgc aagcctttg agctcttcc tcaagcacc tcgtcggag gactgcata cggtttata ccaagctta aaaggctag acatcgtgc cgttggttt gtaacgtcg agcaggtca ggatcttga gaggactcg gtgccattc aaccagggg ttaacagat gcaagctac tctcatgca aagacttcg atacactct acagagact gcactacgg ccagttcca ggcccggac gtcctccca agcgagcgc cggtgggcc tcagcctcg cagcatcgc atcttcaac agcaacagc tcaagaaac aacgcctcg acaacggtg attatccgt acgaaaacg gacacatgg gtgccgagt ttccacttg acataatga tgcgaagag atatgtcaa gactagaaa aatcgccgc ttttggcga acggttaca atcatcctc ctcatttaa gtcacatgc agtttatgc aacttcctc atccggcgg ctgcacatt cggcactct tgtcgcctg cgatgcccc acaatctca caagacacg acggctccg ttataaaga accaaggaa tgcccacga tggaggcta tagcgaggt caggcattt gggaaaact gcagggccg cgtacgaag acatcgtca aacatttag aaaggctga gggaggagc ggtgcgatc ccgacagac cgctgccct tggaccaga aagccaggg acctcagtc cacgaaacg gctctccga ccgatcaca gtcccgtgc tgaatctgt cgaagagtg gcggcggca gcgccggct cgttagggg acggcgacc attcgggca gcgaagccg acgggccgg acgtcccgc cgtcgccgc gctccccca ggtccgccg ccgaggacg aggacgacg acaacatct ccgacccgg aggacgacg acgacaagg atcaagaca tggacgagg gcgacctgc ccctcccgg cggcccccg ggggcgact cccagcagg cagccctga actattcga ctcttgcga gtgcggtgg cttcggcca acggtccta gtcaagacc cgagcattt cctcgacgg agacgctgc tccgcaaca tccagggac tcctgaagg tggcagcgg acaacgccc gccagcagg aacgccaga tcaactacg agaaaggta caaatagca acatggtgt atctgctgt cgctagccc agtactata tgtatctag cagctacgc aaaaatgct aaattctga tgattgagt gactcggtg taatatttt gtaaataca taaagtgcc gctggaaaa tcggaaatt taaattgtg ggcgaaaat tgcgtgatc tgagctggt agagtagtg aatttaagt ttcgaattt tccaacgta cgattgcat agcaagtta gtattgagt ttgtataaa tgacggcac tcgagtgca catatcgac ttgtactct aaaaataag tagctttac taggtgtac atacttaag cgtaatctg tggatagaa gcagatatt ttctagcaa aggtaatgt tagagtgac gactgttga gagtactaa aggacgcct cagataaga atctgtgtg tgtttgaac ctgacccgg tgtttcagt gatggaatt tggttattc tgtcggctg gttgttagt atattatta gtaatgtgc cattacttc cagtgttgt tagagtaga tgtttattt aaaaattag ttaatttaa cttaaatat ttatatatt taaaataat ccgaaaatt ttgatatat tctcctcct gtatattta cccggaata taacaaaat tttccctgc accaaaaaa attcagtga cgacttcct tccaacgga actcgatct gacgtctag cattaggtt tatgtgtga tgtacatgt taacaattc aaaattgaa aataaacga aattactat at
- >TC032755 RB
cacagaaat ccccataaa aatattata tgctctccc aaaggcccc gccttccgg cacttgcag cactttttc ccaatgttt ttatttatt acatttgta cccggcgag tgtaccggt cgtgtccgc accagtcga gtacagagt gtaccccgt gcgcacgac gccccgccc ggccatcca tccgagccc tcactgttg cgactcctc ttctgggtg atggactct ggtgcagtg ctctaggtg ttcgcgtcg cgatggaca actccggga tggactccg ccagcgagc acagcagcc acgcgtcct cgggctcgc cggccgtcg ccgtcaagc cgtcgccgt cgccgcccg ccgccaaca gcaacacgc accaggtgc acagcccgc gccaggcca gcccgctgg ggccgccca tgtccatgg cgcacccgc tgagcccgc cgcccgtca gctcggcca gtctggccc gcagcatgg ctctgggcc acctgccgc cccccggct tgggcttgc tcaactcgc tgggggtgc tgcacagcc ctctggacc tcatggcgc atcacgcac cgccacggt cctacaaca gcccgccgc cgatttcca cctccgatc ccacggcta atgagtgca aactggttg attacagag gacagaagg tcgccgctt ttatcatcg ccggcgata ccatgctgt gtcttccgc aagcctttg agctcttcc tcaagcacc tcgtcggag gactgcata cggtttata ccaagctta aaaggctag acatcgtgc cgttggttt gtaacgtcg agcaggtca ggatcttga gaggactcg gtgccattc aaccagggg ttaacagat gcaagctac tctcatgca aagacttcg atacactct acagagact gcactacgg ccagttcca ggcccggac gtcctccca agcgagcgc cggtgggcc tcagcctcg cagcatcgc atcttcaac agcaacagc tcaagaaac aacgcctcg acaacggtg attatccgt acgaaaacg gacacatgg gtgatatgt caagactag aaaaatcgc cgcttttgg cgaacggtt acaatcatc ctcctcatt taagtcaca tgcagttta tgcaacttc ctcatccgg cggctgcac attcggcac tcttgtcgc ctgcgatgc cccacaatc tcacaagac acgacggct ccgttataa agaaccaag gaatgccca cgatggagg ctatagcga ggtcaggca tttgggaaa actgcaggg ccgcgtacg aagacatcg tcaaacatt tagaaaggc tgagggagg agcggtgcg atcccgaca gaccgctgc ccttggacc agaaagcca gggacctca gtccacgaa acggctctc cgaccgatc acagtcccg tgctgaatc tgtcgaaga gtggcggcg gcagcgccg gctcgttag gggacggcg accattcgg gcagcgaag ccgacgggc cggacgtcc cgccgtcgc cgcgctccc ccaggtccg ccgccgagg acgaggacg acgacaaca tctccgacc cggaggacg acgacgaca aggatcaag acatggacg agggcgacc tgcccctcc cggcggccc ccgggggcg actcccagc aggcagccc tgaactatt cgactcttg cgagtgcgg tggcttcgg ccaacggtc ctagtcaag acccgagca tttcctcga cggagacgc tgctccgca acatccagg gactcctga aggtggcag cggacaacg cccgccagc aggaacgcc agatcaact acgagaaag gtacaaata gcaacatgg tgtatctgc tgtcgctag cccagtact atatgtatc tagcagcta cgcaaaaat gctaaattc tgatgattg agtgactcg gtgtaatat tttgtaaat acataaagt gccgctgga aaatcggaa atttaaatt gtgggcgaa aattgcgtg atctgagct ggtagagta gtgaattta agtttcgaa ttttccaac gtacgattg catagcaag ttagtattg agtttgtat aaatgacgg cactcgagt gcacatatc gacttgtac tctaaaaat aagtagctt tactaggtg tacatactt aagcgtaat ctgtggata gaagcagat attttctag caaaggtaa tgttagagt gacgactgt tgagagtac taaaggacg cctcagata agaatctgt gtgtgtttg aacctgacc cggtgtttc agtgatgga atttggtta ttctgtcgg ctggttgtt agtatatta ttagtaatg tgccattac ttccagtgt tgttagagt agatgttta tttaaaaat tagttaatt taacttaaa tatttatat atttaaaat aatccgaaa attttgata tattctcct cctgtatat ttacccgga atataacaa aattttccc tgcaccaaa aaaattcag tgacgactt ccttccaac ggaactcga tctgacgtc tagcattag gtttatgtg tgatgtaca tgttaacaa ttcaaaatt gaaaataaa cgaaattac tatat
- >TC032755 RC
cacagaaat ccccataaa aatattata tgctctccc aaaggcccc gccttccgg cacttgcag cactttttc ccaatgttt ttatttatt acatttgta cccggcgag tgtaccggt cgtgtccgc accagtcga gtacagagt gtaccccgt gcgcacgac gccccgccc ggccatcca tccgagccc tcactgttg cgactcctc ttctgggtg atggactct ggtgcagtg ctctaggtg ttcgcgtcg cgatggaca actccggga tggactccg ccagcgagc acagcagcc acgcgtcct cgggctcgc cggccgtcg ccgtcaagc cgtcgccgt cgccgcccg ccgccaaca gcaacacgc accaggtgc acagcccgc gccaggcca gcccgctgg ggccgccca tgtccatgg cgcacccgc tgagcccgc cgcccgtca gctcggcca gtctggccc gcagcatgg ctctgggcc acctgccgc cccccggct tgggcttgc tcaactcgc tgggggtgc tgcacagcc ctctggacc tcatggcgc atcacgcac cgccacggt cctacaaca gcccgccgc cgatttcca cctccgatc ccacggcta atgagtgca aactggttg attacagag gacagaagg tcgccgctt ttatcatcg ccggcgata ccatgctgt gtcttccgc aagcctttg agctcttcc tcaagcacc tcgtcggag gactgcata cggtttata ccaagctta aaaggctag acatcgtgc cgttggttt gtaacgtcg agcaggtca ggatcttga gaggactcg gtgccattc aaccagggg ttaacagat gcaagctac tctcatgca aagacttcg atacactct acagagact gcactacgg ccagttcca ggcccggac gtcctccca agcgagcgc cggtgggcc tcagcctcg cagcatcgc atcttcaac agcaacagc tcaagaaac aacgcctcg acaacggtg attatccgt acgaaaacg gacacatgg gtgatatgt caagactag aaaaatcgc cgcttttgg cgaacggtt acaatcatc ctcctcatt taagtcaca tgcagttta tgcaacttc ctcatccgg cggctgcac attcggcac tcttgtcgc ctgcgatgc cccacaatc tcacaagac acgacggct ccgttataa agaaccaag gaatgccca cgatggagg ctatagcga ggtcaggca tttgggaaa actgcaggg ccgcgtacg aagacatcg tcaaacatt tagaaaggc tgagggagg agcggtgcg atcccgaca gaccgctgc ccttggacc agaaagcca gggacctca gtccacgaa acggctctc cgaccgatc acagtcccg tgctgaatc tgtcgaaga gtggcggcg gcagcgccg gctcgttag gggacggcg accattcgg gcagcgaag ccgacgggc cggacgtcc cgccgtcgc cgcgctccc ccaggtccg ccgccgagg acgaggacg acgacaaca tctccgacc cggaggacg acgacgaca aggatcaag acatggacg agggcgacc tgcccctcc cggcggccc ccgggggcg actcccagc aggcagccc tgaactatt cgactcttg cgagtgcgg tggcttcgg ccaacggtc ctagtcaag acccgagca tttcctcga cggagacgc tgctccgca acatccagg gactcctga aggtggcag cggacaacg cccgccagc aggaacgcc agatcaact acgagaaag ccgaattga agatggacg tgcttcggg aaagagagg tgaaagaca gcttagaaa gacaactgg tcgacgaac aaaaaatgc gaggggcgg ctcatccaa gtgttaaac ggacacagc tcgaaatcc gaatcatgc aaacttcct gtgtccagg tctggcctg ccttcttag cccccggcc gtttcgcga cgacaagga cgaccagtg cgagggagg cgcgcaaca aggacctcc gaggtctta ttcaaatag agccgaggg cagggtcgt gcactcctg gggagaacc cgctcgacg ggaagcccg actccagat tgaaattca ttattagct cgcaaactg atgcatcac aattaacga aaatattcg ccttttcag cactggtgc agaagcggc tccggaggg agcgtcgcg cacgtcggc gactccaag atcaactgg aggcggaag tcaaaagac gcaccctga tggaagagg cccttaagg cggcggggg ccgcagaac agctcagga taattaatg aaaaagtgg cgccgagcg agcagaagc tgagcaacc cgaaggtga ccacaagct caaccccaa accagagca tctcgtcgg agcgtgaga gggtatcgg aacgtgtcg aaaggatgg aaagagaac gtgtggaaa gcccaccgc cggcatatg ggcagcaaa ctgtccgag agccgccgc cacctccgc ccgaaaata agccgtggg gctattccg gaatcgacc tcatgaata ccggagccg ccttctggc agaattact ccgaatcct tagcacaag aactcgaat tggagagaa aatcccggc agcaacaag ttgagagag acgtgaagt caccgcttc aagatcgtt ccgggtatt acaaaaatt ccatgctgt tcacaagca cggctacat aaacataac gtacgagtt ttgcttaga taaaagtat aaagtaggt tttagatca gagcgtaca gtgcttagc aatgtttcg attggtatt atgcattcg ttttaaacg agatattaa gttttttcc ttaaaatac gtccagtgt cgtggagca tatcagtta gtgattgtt tgattttta agactgatt ttgggcatg gagaggaac gataacaat ttcagtact taacttttc ttattgctc agaattcgc acttcgtgt acataaaca cacctgtat ataatttct attttgtaa tccaaccca attacttta ctatgactg tttggaatt attctaatt aaatatctc cgctttaca tgtacatat gagcgtcag tataaacct gtagaaaag tcatttttc atgtaacaa cgcagaata gaagattgt atattaaaa tttttataa agttaaagt tttgcatat ccatgtgaa ctttctacg atatgatat gaacataaa attcatttg tacattatt tcgtaatct gaaatgttt tacacagtg taggatttt ctgacgaaa agtatacgc cattttgac aatcattgt cagaaattt ttcaaatat caatattgt cgtttgttt acactcagt attattcag attaggtgc aatactgaa ccacaaatt gtacgaacg gtagtgttc ttaatatag cacagtttt tttcttact tgcttgtat cataagtag gctagttta taaactgtt cgtgttaga ctatgtaag aggcagtgt gtatgtatt tattgtaaa taaatgtat gtgtgtgcc
