Status
Please wait ...
Gene information
General information
TC032756
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007634,TC007633
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1991560..1997497
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032756
    iBeetle screen iB_04356

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032756 RA
      MPFPVKSHS ILFETLLKR LAEKGHEVD YVTHFLSEK 
      TPERYNELS IRGSLPLFH NNFSVKALK DHSVYETIK 
      FVTTSGGVD ANSAIMKTA VLQSLKNTT KQYDLIITH 
      LFGSDAMLG FGHLFNAPI VSITTCAVL PWAHGRIAN 
      PDNPSYVPN FYTSFLPKM NLYERIENT ILWIMTRIW 
      YNYLTMAPS NRLVKDFFG PDTPSLENL IQNTSLVLV 
      NSHFSMQQV RPTVPNFIE VGGLHIREP QPLPKDLEN 
      LVSNNTFGV VYLSMGSMV ITETFEPEI LQAMFDAFA 
      ELPYTVLWK ASPEKFPKG LTIPENIHF KTWMPQIDI 
      LCHPNVKLF ISHGGLLGS QEAVYCAVP RIGIPLYGD 
      QEANIHKSE KLGIAIKLA YGSITKDSF LETVKRVLE 
      DLSYRHNVQ KISQIFKDR PMSPLDTAV YWVEYVIKY 
      KGAPHLRSV GADLPWYQY YLIDVAVIL LFGLFVKFQ 
      DLENLVSNN TFGVVYLSM GSMVRTESF KPEILQAMF 
      DAFAELPYT VLWKASPEK FPKGLKIPE NIHFKTWMP 
      QIDILCHPN VKLFISHGG LLGSQEAVY CAIPRIGIP 
      FFDDQELNI VTYNELSLY GTLPDHVNN LSIEGMQNQ 
      TIYQRIKFM TRTAGVEAF SRILQTPVL RNLRNTTKK 
      YHLIIVHQF GCDVMLGFG FLFNAPVIS YVTSVSPPW 
      IDDRIGNPD NPSYIQSHF SRSSAKMSL YERVENTIF 
      WISARFWYS FFSGRSDQL VKDFFGHKT PSLENLIRN 
      DSLVLVNSH FSLQQVRPL VPNFIEVGG LHIREPQPL 
      PKDLENLVS NNKFGVIYL SMGSMIMTE TYDPEILQA 
      MFDAFAELP YTVLWKASP EKFPKGLKI PENIHFKMW 
      MPQIDILCH PNVKLFISH GGMLGSQEA VYCAVPRIG 
      VPIYADQER NIVTSEKLG IAKKLSYDH INKNTFLHT 
      IKELIEDLK YVWLKKKLL LSNFC
    • >TC032756 RB
      MPFPVKSHS ILFETLLKR LAEKGHEVD YVTHFLSEK 
      TPERYNELS IRGSLPLFH NNFSVKALK DHSVYETIK 
      FVTTSGGVD ANSAIMKTA VLQSLKNTT KQYDLIITH 
      LFGSDAMLG FGHLFNAPI VSITTCAVL PWAHGRIAN 
      PDNPSYVPN FYTSFLPKM NLYERIENT ILWIMTRIW 
      YNYLTMAPS NRLVKDFFG PDTPSLENL IQNTSLVLV 
      NSHFSMQQV RPTVPNFIE VGGLHIREP QPLPKDLEN 
      LVSNNTFGV VYLSMGSMV ITETFEPEI LQAMFDAFA 
      ELPYTVLWK ASPEKFPKG LTIPENIHF KTWMPQIDI 
      LCHPNVKLF ISHGGLLGS QEAVYCAVP RIGIPLYGD 
      QEANIHKSE KLGIAIKLA YGSITKDSF LETVKRVLE 
      DLSYRHNVQ KISQIFKDR PMSPLDTAV YWVEYVIKY 
      KGAPHLRSV GADLPWYQY YLIDVAVIL LFGLFVKFQ 
      DLENLVSNN TFGVVYLSM GSMVRTESF KPEILQAMF 
      DAFAELPYT VLWKASPEK FPKGLKIPE NIHFKTWMP 
      QIDILCHPN VKLFISHGG LLGSQEAVY CAIPRIGIP 
      FFDDQELNI VTSEKLGIA KKLSYGHIN KNTLLNTIT 
      ELFEDLKYN ELSLYGTLP DHVNNLSIE GMQNQTIYQ 
      RIKFMTRTA GVEAFSRIL QTPVLRNLR NTTKKYHLI 
      IVHQFGCDV MLGFGFLFN APVISYVTS VSPPWIDDR 
      IGNPDNPSY IQSHFSRSS AKMSLYERV ENTIFWISA 
      RFWYSFFSG RSDQLVKDF FGHKTPSLE NLIRNDSLV 
      LVNSHFSLQ QVRPLVPNF IEVGGLHIR EPQPLPKDL 
      ENLVSNNKF GVIYLSMGS MIMTETYDP EILQAMFDA 
      FAELPYTVL WKASPEKFP KGLKIPENI HFKMWMPQI 
      DILCHPNVK LFISHGGML GSQEAVYCA VPRIGVPIY 
      ADQERNIVT SEKLGIAKK LSYDHINKN TFLHTIKEL 
      IEDLKYVWL KKKLLLSNF C
    CDS sequence
    • >TC032756 RA
      atgcctttt cctgtgaaa agtcactcg attttattc 
      gagaccctc ctgaaacgt cttgctgaa aaagggcat 
      gaagttgat tacgtcacg cattttctt tccgaaaaa 
      accccagaa agatacaac gagttaagc attcgtgga 
      agccttcca ttatttcac aataatttt tcagttaaa 
      gctttaaag gatcattcc gtttacgaa acgataaaa 
      tttgtgaca acgtctgga ggagtggat gctaattcc 
      gcaattatg aaaactgca gttttgcaa agtctgaaa 
      aacacaacg aaacaatac gacttaatt attacccat 
      ctttttggt tctgatgca atgttgggt tttgggcac 
      ttatttaac gcccccatt gtgtctata acgacctgt 
      gcggttctt ccttgggca cacggacga atcgcaaat 
      ccggataat ccgtcttat gtcccaaat ttttacacc 
      agttttctc cccaagatg aatttgtac gaaagaatc 
      gagaataca attttgtgg ataatgacc agaatttgg 
      tataactac ctgaccatg gctccctca aaccgcttg 
      gttaaagat tttttcgga cctgataca ccgtctttg 
      gaaaatctc attcaaaac accagtctt gtgctcgtt 
      aatagtcat ttcagcatg cagcaggtt aggccaact 
      gtgccaaat ttcatagaa gttgggggg ctccacata 
      cgcgaacct cagccacta ccaaaagat ttggaaaat 
      ttagtcagc aataacacg ttcggagtc gtttacttg 
      tcgatgggt tcgatggtt ataactgaa accttcgaa 
      cctgaaatt ctccaagca atgtttgac gcttttgct 
      gaattgccg tacacagtt ttgtggaaa gcctcgcct 
      gaaaaattt cccaagggc ttgacaatt cccgaaaat 
      attcacttt aaaacgtgg atgcctcag attgatatt 
      ctatgccac cctaatgtg aaattattt attagtcat 
      ggaggtttg ttaggtagc caggaagcc gtctattgt 
      gctgttcca cgaattgga attccactt tatggtgac 
      caagaagct aatattcac aagtctgaa aaattggga 
      attgcgata aaactggct tatgggagt ataaccaaa 
      gacagtttt cttgaaact gttaagcga gtccttgag 
      gatttgagc tacaggcac aacgttcag aaaatctcc 
      caaatattc aaagatcgt cccatgtcg cctttggac 
      actgcagtt tattgggtc gaatatgta attaaatac 
      aaaggggct ccacatttg cgttctgtg ggtgccgac 
      ttgccgtgg tatcagtat tatttaata gatgttgct 
      gttatttta ctctttggt ttgtttgtg aagtttcag 
      gatttggaa aatttagtc agcaataac acgttcgga 
      gtcgtttac ttatcaatg ggttcaatg gttaggact 
      gaaagcttc aaacctgaa attctccaa gccatgttt 
      gatgctttt gctgaattg ccttatact gttttatgg 
      aaggcatcg cctgaaaag tttcccaaa ggcttgaaa 
      attcccgaa aatattcac tttaaaacg tggatgcct 
      cagatcgat attctatgc catcctaac gtgaaatta 
      tttattagt catggaggg ttattaggt agccaggaa 
      gcagtctat tgcgctatt cccagaata ggaattcca 
      ttttttgac gaccaagaa ctcaatata gtcacatac 
      aatgaatta agtttatac ggaactctc ccagatcat 
      gttaacaat ttatcgata gaaggaatg cagaatcaa 
      actatttac caaaggatc aaatttatg actcggact 
      gcaggcgtt gaggccttt tctaggata ttgcaaacg 
      ccagtttta cgaaatctg aggaacaca acaaaaaaa 
      taccactta attatcgtt caccaattc ggttgtgat 
      gttatgtta ggatttgga tttttgttc aatgctccc 
      gtcatttca tacgttaca agtgttagt cctccttgg 
      atagacgac aggatcgga aatcctgat aacccctct 
      tacatacaa agtcatttc tcacgttca agcgccaag 
      atgagtttg tacgaaaga gtcgaaaat acgattttt 
      tggataagt gccagattt tggtatagt ttcttttct 
      ggtcgttcc gatcaatta gttaaggat tttttcgga 
      cacaagaca ccgtcttta gagaatctt attcgaaac 
      gatagtctt gtgctcgtt aatagtcat ttcagtttg 
      cagcaggtt aggccactt gtgccaaat tttatcgaa 
      gttgggggg ctccacata cgcgaaccc cagccacta 
      ccaaaagat ttggaaaat ttggttagc aataacaag 
      ttcggggtc atttatttg tcgatgggt tcaatgatt 
      atgactgaa acctacgat cctgaaatt cttcaggca 
      atgtttgat gcttttgct gaattgccg tacactgtt 
      ttatggaaa gcatcgcct gaaaaattt cccaaaggc 
      ttgaaaatt cccgaaaat attcacttt aaaatgtgg 
      atgcctcag attgatatt ctatgccac cctaatgtg 
      aaattattt attagccat ggtggtatg ttaggtagt 
      caggaagca gtctattgt gctgttccc agaatagga 
      gttccaatt tatgccgac caggagcgc aatattgtc 
      acatctgag aaattagga attgcgaaa aaactctca 
      tacgaccat ataaacaaa aacactttt ctccacact 
      attaaagag cttattgaa gatttaaag tacgtctgg 
      ttgaaaaaa aaactttta ttgagtaat ttttgttag
    • >TC032756 RB
      atgcctttt cctgtgaaa agtcactcg attttattc 
      gagaccctc ctgaaacgt cttgctgaa aaagggcat 
      gaagttgat tacgtcacg cattttctt tccgaaaaa 
      accccagaa agatacaac gagttaagc attcgtgga 
      agccttcca ttatttcac aataatttt tcagttaaa 
      gctttaaag gatcattcc gtttacgaa acgataaaa 
      tttgtgaca acgtctgga ggagtggat gctaattcc 
      gcaattatg aaaactgca gttttgcaa agtctgaaa 
      aacacaacg aaacaatac gacttaatt attacccat 
      ctttttggt tctgatgca atgttgggt tttgggcac 
      ttatttaac gcccccatt gtgtctata acgacctgt 
      gcggttctt ccttgggca cacggacga atcgcaaat 
      ccggataat ccgtcttat gtcccaaat ttttacacc 
      agttttctc cccaagatg aatttgtac gaaagaatc 
      gagaataca attttgtgg ataatgacc agaatttgg 
      tataactac ctgaccatg gctccctca aaccgcttg 
      gttaaagat tttttcgga cctgataca ccgtctttg 
      gaaaatctc attcaaaac accagtctt gtgctcgtt 
      aatagtcat ttcagcatg cagcaggtt aggccaact 
      gtgccaaat ttcatagaa gttgggggg ctccacata 
      cgcgaacct cagccacta ccaaaagat ttggaaaat 
      ttagtcagc aataacacg ttcggagtc gtttacttg 
      tcgatgggt tcgatggtt ataactgaa accttcgaa 
      cctgaaatt ctccaagca atgtttgac gcttttgct 
      gaattgccg tacacagtt ttgtggaaa gcctcgcct 
      gaaaaattt cccaagggc ttgacaatt cccgaaaat 
      attcacttt aaaacgtgg atgcctcag attgatatt 
      ctatgccac cctaatgtg aaattattt attagtcat 
      ggaggtttg ttaggtagc caggaagcc gtctattgt 
      gctgttcca cgaattgga attccactt tatggtgac 
      caagaagct aatattcac aagtctgaa aaattggga 
      attgcgata aaactggct tatgggagt ataaccaaa 
      gacagtttt cttgaaact gttaagcga gtccttgag 
      gatttgagc tacaggcac aacgttcag aaaatctcc 
      caaatattc aaagatcgt cccatgtcg cctttggac 
      actgcagtt tattgggtc gaatatgta attaaatac 
      aaaggggct ccacatttg cgttctgtg ggtgccgac 
      ttgccgtgg tatcagtat tatttaata gatgttgct 
      gttatttta ctctttggt ttgtttgtg aagtttcag 
      gatttggaa aatttagtc agcaataac acgttcgga 
      gtcgtttac ttatcaatg ggttcaatg gttaggact 
      gaaagcttc aaacctgaa attctccaa gccatgttt 
      gatgctttt gctgaattg ccttatact gttttatgg 
      aaggcatcg cctgaaaag tttcccaaa ggcttgaaa 
      attcccgaa aatattcac tttaaaacg tggatgcct 
      cagatcgat attctatgc catcctaac gtgaaatta 
      tttattagt catggaggg ttattaggt agccaggaa 
      gcagtctat tgcgctatt cccagaata ggaattcca 
      ttttttgac gaccaagaa ctcaatata gtcacgtca 
      gagaaatta ggaattgca aaaaagctc tcatatggt 
      catataaat aaaaatact ttgctgaac actattaca 
      gagcttttt gaagattta aaatacaat gaattaagt 
      ttatacgga actctccca gatcatgtt aacaattta 
      tcgatagaa ggaatgcag aatcaaact atttaccaa 
      aggatcaaa tttatgact cggactgca ggcgttgag 
      gccttttct aggatattg caaacgcca gttttacga 
      aatctgagg aacacaaca aaaaaatac cacttaatt 
      atcgttcac caattcggt tgtgatgtt atgttagga 
      tttggattt ttgttcaat gctcccgtc atttcatac 
      gttacaagt gttagtcct ccttggata gacgacagg 
      atcggaaat cctgataac ccctcttac atacaaagt 
      catttctca cgttcaagc gccaagatg agtttgtac 
      gaaagagtc gaaaatacg attttttgg ataagtgcc 
      agattttgg tatagtttc ttttctggt cgttccgat 
      caattagtt aaggatttt ttcggacac aagacaccg 
      tctttagag aatcttatt cgaaacgat agtcttgtg 
      ctcgttaat agtcatttc agtttgcag caggttagg 
      ccacttgtg ccaaatttt atcgaagtt ggggggctc 
      cacatacgc gaaccccag ccactacca aaagatttg 
      gaaaatttg gttagcaat aacaagttc ggggtcatt 
      tatttgtcg atgggttca atgattatg actgaaacc 
      tacgatcct gaaattctt caggcaatg tttgatgct 
      tttgctgaa ttgccgtac actgtttta tggaaagca 
      tcgcctgaa aaatttccc aaaggcttg aaaattccc 
      gaaaatatt cactttaaa atgtggatg cctcagatt 
      gatattcta tgccaccct aatgtgaaa ttatttatt 
      agccatggt ggtatgtta ggtagtcag gaagcagtc 
      tattgtgct gttcccaga ataggagtt ccaatttat 
      gccgaccag gagcgcaat attgtcaca tctgagaaa 
      ttaggaatt gcgaaaaaa ctctcatac gaccatata 
      aacaaaaac acttttctc cacactatt aaagagctt 
      attgaagat ttaaagtac gtctggttg aaaaaaaaa 
      cttttattg agtaatttt tgttag
    mRNA sequence
    • >TC032756 RA
      ataagccgt gtccgcaag ctgaaaatg ttatttatt 
      tatttattt attattgtg ccgagcgtt tcaataact 
      gaaacggcc aacattttg ggaattatg ccttttcct 
      gtgaaaagt cactcgatt ttattcgag accctcctg 
      aaacgtctt gctgaaaaa gggcatgaa gttgattac 
      gtcacgcat tttctttcc gaaaaaacc ccagaaaga 
      tacaacgag ttaagcatt cgtggaagc cttccatta 
      tttcacaat aatttttca gttaaagct ttaaaggat 
      cattccgtt tacgaaacg ataaaattt gtgacaacg 
      tctggagga gtggatgct aattccgca attatgaaa 
      actgcagtt ttgcaaagt ctgaaaaac acaacgaaa 
      caatacgac ttaattatt acccatctt tttggttct 
      gatgcaatg ttgggtttt gggcactta tttaacgcc 
      cccattgtg tctataacg acctgtgcg gttcttcct 
      tgggcacac ggacgaatc gcaaatccg gataatccg 
      tcttatgtc ccaaatttt tacaccagt tttctcccc 
      aagatgaat ttgtacgaa agaatcgag aatacaatt 
      ttgtggata atgaccaga atttggtat aactacctg 
      accatggct ccctcaaac cgcttggtt aaagatttt 
      ttcggacct gatacaccg tctttggaa aatctcatt 
      caaaacacc agtcttgtg ctcgttaat agtcatttc 
      agcatgcag caggttagg ccaactgtg ccaaatttc 
      atagaagtt ggggggctc cacatacgc gaacctcag 
      ccactacca aaagatttg gaaaattta gtcagcaat 
      aacacgttc ggagtcgtt tacttgtcg atgggttcg 
      atggttata actgaaacc ttcgaacct gaaattctc 
      caagcaatg tttgacgct tttgctgaa ttgccgtac 
      acagttttg tggaaagcc tcgcctgaa aaatttccc 
      aagggcttg acaattccc gaaaatatt cactttaaa 
      acgtggatg cctcagatt gatattcta tgccaccct 
      aatgtgaaa ttatttatt agtcatgga ggtttgtta 
      ggtagccag gaagccgtc tattgtgct gttccacga 
      attggaatt ccactttat ggtgaccaa gaagctaat 
      attcacaag tctgaaaaa ttgggaatt gcgataaaa 
      ctggcttat gggagtata accaaagac agttttctt 
      gaaactgtt aagcgagtc cttgaggat ttgagctac 
      aggcacaac gttcagaaa atctcccaa atattcaaa 
      gatcgtccc atgtcgcct ttggacact gcagtttat 
      tgggtcgaa tatgtaatt aaatacaaa ggggctcca 
      catttgcgt tctgtgggt gccgacttg ccgtggtat 
      cagtattat ttaatagat gttgctgtt attttactc 
      tttggtttg tttgtgaag tttcaggat ttggaaaat 
      ttagtcagc aataacacg ttcggagtc gtttactta 
      tcaatgggt tcaatggtt aggactgaa agcttcaaa 
      cctgaaatt ctccaagcc atgtttgat gcttttgct 
      gaattgcct tatactgtt ttatggaag gcatcgcct 
      gaaaagttt cccaaaggc ttgaaaatt cccgaaaat 
      attcacttt aaaacgtgg atgcctcag atcgatatt 
      ctatgccat cctaacgtg aaattattt attagtcat 
      ggagggtta ttaggtagc caggaagca gtctattgc 
      gctattccc agaatagga attccattt tttgacgac 
      caagaactc aatatagtc acatacaat gaattaagt 
      ttatacgga actctccca gatcatgtt aacaattta 
      tcgatagaa ggaatgcag aatcaaact atttaccaa 
      aggatcaaa tttatgact cggactgca ggcgttgag 
      gccttttct aggatattg caaacgcca gttttacga 
      aatctgagg aacacaaca aaaaaatac cacttaatt 
      atcgttcac caattcggt tgtgatgtt atgttagga 
      tttggattt ttgttcaat gctcccgtc atttcatac 
      gttacaagt gttagtcct ccttggata gacgacagg 
      atcggaaat cctgataac ccctcttac atacaaagt 
      catttctca cgttcaagc gccaagatg agtttgtac 
      gaaagagtc gaaaatacg attttttgg ataagtgcc 
      agattttgg tatagtttc ttttctggt cgttccgat 
      caattagtt aaggatttt ttcggacac aagacaccg 
      tctttagag aatcttatt cgaaacgat agtcttgtg 
      ctcgttaat agtcatttc agtttgcag caggttagg 
      ccacttgtg ccaaatttt atcgaagtt ggggggctc 
      cacatacgc gaaccccag ccactacca aaagatttg 
      gaaaatttg gttagcaat aacaagttc ggggtcatt 
      tatttgtcg atgggttca atgattatg actgaaacc 
      tacgatcct gaaattctt caggcaatg tttgatgct 
      tttgctgaa ttgccgtac actgtttta tggaaagca 
      tcgcctgaa aaatttccc aaaggcttg aaaattccc 
      gaaaatatt cactttaaa atgtggatg cctcagatt 
      gatattcta tgccaccct aatgtgaaa ttatttatt 
      agccatggt ggtatgtta ggtagtcag gaagcagtc 
      tattgtgct gttcccaga ataggagtt ccaatttat 
      gccgaccag gagcgcaat attgtcaca tctgagaaa 
      ttaggaatt gcgaaaaaa ctctcatac gaccatata 
      aacaaaaac acttttctc cacactatt aaagagctt 
      attgaagat ttaaagtac gtctggttg aaaaaaaaa 
      cttttattg agtaatttt tgttagata caagcaaaa 
      cattgaaag aa
    • >TC032756 RB
      ataagccgt gtccgcaag ctgaaaatg ttatttatt 
      tatttattt attattgtg ccgagcgtt tcaataact 
      gaaacggcc aacattttg ggaattatg ccttttcct 
      gtgaaaagt cactcgatt ttattcgag accctcctg 
      aaacgtctt gctgaaaaa gggcatgaa gttgattac 
      gtcacgcat tttctttcc gaaaaaacc ccagaaaga 
      tacaacgag ttaagcatt cgtggaagc cttccatta 
      tttcacaat aatttttca gttaaagct ttaaaggat 
      cattccgtt tacgaaacg ataaaattt gtgacaacg 
      tctggagga gtggatgct aattccgca attatgaaa 
      actgcagtt ttgcaaagt ctgaaaaac acaacgaaa 
      caatacgac ttaattatt acccatctt tttggttct 
      gatgcaatg ttgggtttt gggcactta tttaacgcc 
      cccattgtg tctataacg acctgtgcg gttcttcct 
      tgggcacac ggacgaatc gcaaatccg gataatccg 
      tcttatgtc ccaaatttt tacaccagt tttctcccc 
      aagatgaat ttgtacgaa agaatcgag aatacaatt 
      ttgtggata atgaccaga atttggtat aactacctg 
      accatggct ccctcaaac cgcttggtt aaagatttt 
      ttcggacct gatacaccg tctttggaa aatctcatt 
      caaaacacc agtcttgtg ctcgttaat agtcatttc 
      agcatgcag caggttagg ccaactgtg ccaaatttc 
      atagaagtt ggggggctc cacatacgc gaacctcag 
      ccactacca aaagatttg gaaaattta gtcagcaat 
      aacacgttc ggagtcgtt tacttgtcg atgggttcg 
      atggttata actgaaacc ttcgaacct gaaattctc 
      caagcaatg tttgacgct tttgctgaa ttgccgtac 
      acagttttg tggaaagcc tcgcctgaa aaatttccc 
      aagggcttg acaattccc gaaaatatt cactttaaa 
      acgtggatg cctcagatt gatattcta tgccaccct 
      aatgtgaaa ttatttatt agtcatgga ggtttgtta 
      ggtagccag gaagccgtc tattgtgct gttccacga 
      attggaatt ccactttat ggtgaccaa gaagctaat 
      attcacaag tctgaaaaa ttgggaatt gcgataaaa 
      ctggcttat gggagtata accaaagac agttttctt 
      gaaactgtt aagcgagtc cttgaggat ttgagctac 
      aggcacaac gttcagaaa atctcccaa atattcaaa 
      gatcgtccc atgtcgcct ttggacact gcagtttat 
      tgggtcgaa tatgtaatt aaatacaaa ggggctcca 
      catttgcgt tctgtgggt gccgacttg ccgtggtat 
      cagtattat ttaatagat gttgctgtt attttactc 
      tttggtttg tttgtgaag tttcaggat ttggaaaat 
      ttagtcagc aataacacg ttcggagtc gtttactta 
      tcaatgggt tcaatggtt aggactgaa agcttcaaa 
      cctgaaatt ctccaagcc atgtttgat gcttttgct 
      gaattgcct tatactgtt ttatggaag gcatcgcct 
      gaaaagttt cccaaaggc ttgaaaatt cccgaaaat 
      attcacttt aaaacgtgg atgcctcag atcgatatt 
      ctatgccat cctaacgtg aaattattt attagtcat 
      ggagggtta ttaggtagc caggaagca gtctattgc 
      gctattccc agaatagga attccattt tttgacgac 
      caagaactc aatatagtc acgtcagag aaattagga 
      attgcaaaa aagctctca tatggtcat ataaataaa 
      aatactttg ctgaacact attacagag ctttttgaa 
      gatttaaaa tacaatgaa ttaagttta tacggaact 
      ctcccagat catgttaac aatttatcg atagaagga 
      atgcagaat caaactatt taccaaagg atcaaattt 
      atgactcgg actgcaggc gttgaggcc ttttctagg 
      atattgcaa acgccagtt ttacgaaat ctgaggaac 
      acaacaaaa aaataccac ttaattatc gttcaccaa 
      ttcggttgt gatgttatg ttaggattt ggatttttg 
      ttcaatgct cccgtcatt tcatacgtt acaagtgtt 
      agtcctcct tggatagac gacaggatc ggaaatcct 
      gataacccc tcttacata caaagtcat ttctcacgt 
      tcaagcgcc aagatgagt ttgtacgaa agagtcgaa 
      aatacgatt ttttggata agtgccaga ttttggtat 
      agtttcttt tctggtcgt tccgatcaa ttagttaag 
      gattttttc ggacacaag acaccgtct ttagagaat 
      cttattcga aacgatagt cttgtgctc gttaatagt 
      catttcagt ttgcagcag gttaggcca cttgtgcca 
      aattttatc gaagttggg gggctccac atacgcgaa 
      ccccagcca ctaccaaaa gatttggaa aatttggtt 
      agcaataac aagttcggg gtcatttat ttgtcgatg 
      ggttcaatg attatgact gaaacctac gatcctgaa 
      attcttcag gcaatgttt gatgctttt gctgaattg 
      ccgtacact gttttatgg aaagcatcg cctgaaaaa 
      tttcccaaa ggcttgaaa attcccgaa aatattcac 
      tttaaaatg tggatgcct cagattgat attctatgc 
      caccctaat gtgaaatta tttattagc catggtggt 
      atgttaggt agtcaggaa gcagtctat tgtgctgtt 
      cccagaata ggagttcca atttatgcc gaccaggag 
      cgcaatatt gtcacatct gagaaatta ggaattgcg 
      aaaaaactc tcatacgac catataaac aaaaacact 
      tttctccac actattaaa gagcttatt gaagattta 
      aagtacgtc tggttgaaa aaaaaactt ttattgagt 
      aatttttgt tagatacaa gcaaaacat tgaaagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY