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Gene information
General information
TC007624 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2104123..2105750
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007624
    iBeetle screen iB_04354

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007624 RA
      MKNVQQEIL TTEDTFVSV VPTRDNTLK VQNCTEDTG 
      ERVQLCKLH EFLTTFELT KITTLHLQN CEISQLPRD 
      VQKLNLKRL VLNHNQLKE VPSCLYSGL EALQVLDLS 
      HNLIKTFDV EPACSRNIV SLKLSHNKI YNFPHWIMT 
      CHCPNLIEI DYSANFLTS VSVARKLTL KKITMNNSQ 
      LLDVDFEFF KRISTLEYL DIGNTNCKL TKQENEFKD 
      ITQLFVKTK WKELKVLKL DHILVSIIP DGLFWIVSL 
      TELHLSHCE LSWIPPEVQ YLTNLEVLD VSHNSICGI 
      PKELAILPK LRIVKASFN LVSSVDDFT NELEILDLY 
      HNELETFPN SIERIKFVD LEQNYVDMS LYKTYGEKR 
      DSFRQMLDD GRADGPRVP PTEVPSSDD DRLSSEGSV 
      CGNDDFYDA DLVDETWDY EEPNVNVGR VRNPEITPS 
      DDEWTGREE IIIREKRTT KPWIDVNED WIFSDVE
    CDS sequence
    • >TC007624 RA
      atgaaaaac gtccaacaa gagatttta actacagag 
      gatacgttt gtgtccgtt gtcccgacc cgtgataac 
      acccttaaa gtgcaaaat tgcactgag gataccggg 
      gagcgagtg cagctctgt aagttgcat gaatttctt 
      accaccttt gaattgact aaaattaca actttgcat 
      cttcaaaat tgcgaaatt agccaactc ccgcgcgat 
      gtacagaaa ttaaattta aagcgcttg gttttgaac 
      cacaatcaa ctaaaagaa gtgcctagt tgtttgtat 
      tccggcttg gaagcgttg caagtgtta gacctgtct 
      cataatctt atcaaaacg tttgatgtc gagccggct 
      tgtagtcgc aatattgtt agtctaaaa ctgtcccat 
      aacaaaatt tacaatttt ccgcactgg atcatgacc 
      tgtcattgc cccaattta atcgaaatt gattacagt 
      gccaatttt ttgaccagc gtgagcgtg gcacgtaag 
      ctaacgctc aaaaaaatt acaatgaac aattcgcaa 
      ttgcttgac gttgatttc gagtttttc aaacgtata 
      tcaacctta gaatatcta gatataggt aacaccaat 
      tgcaaatta acaaaacag gagaacgaa tttaaagac 
      ataactcaa ctattcgtt aaaaccaaa tggaaagaa 
      ctgaaagtt ttgaagttg gaccacata cttgtgtct 
      attatcccg gacggactt ttctggatt gtgtcactg 
      actgagctt cacttaagt cattgcgag ctgagctgg 
      ataccgcca gaggtccag tatttaaca aacttggag 
      gttctggat gtgtctcat aattccatt tgcggaatt 
      ccgaaagaa ctagcgatt ttaccgaag ttgcgaatc 
      gttaaagca tcttttaat cttgtttcg tcagtggat 
      gatttcacg aatgagtta gaaattttg gatttgtat 
      cataatgaa cttgagacg tttccaaat tcaatcgag 
      cgaattaaa ttcgtcgat ttggagcag aattatgtc 
      gatatgtcg ctgtataaa acatatggc gaaaaacgg 
      gattctttc cgccaaatg cttgacgat gggagagct 
      gacggtccg agggttcca ccaacggag gttcccagc 
      agtgacgat gatcggctt tcatcagaa ggcagcgtt 
      tgcggaaat gatgatttt tatgacgct gatttggtt 
      gatgaaacg tgggattat gaggaacct aatgttaat 
      gttggtcgt gtgcggaac ccagaaata acgccctct 
      gatgacgaa tggacgggg agagaagaa ataatcatc 
      agggaaaaa agaacaacc aagccgtgg attgatgta 
      aatgaagac tggatattt tcagatgtt gaatga
    mRNA sequence
    • >TC007624 RA
      actttaatt aaacaacgc gggcaaatt tcaaaagac 
      ggctgagtt cgtgccaac tcgtgcgct gatcggtca 
      acaggtggg gatgacatg cttgttgtt attttccta 
      acctaatta cctaattgt caaaataaa atgaaaaac 
      gtccaacaa gagatttta actacagag gatacgttt 
      gtgtccgtt gtcccgacc cgtgataac acccttaaa 
      gtgcaaaat tgcactgag gataccggg gagcgagtg 
      cagctctgt aagttgcat gaatttctt accaccttt 
      gaattgact aaaattaca actttgcat cttcaaaat 
      tgcgaaatt agccaactc ccgcgcgat gtacagaaa 
      ttaaattta aagcgcttg gttttgaac cacaatcaa 
      ctaaaagaa gtgcctagt tgtttgtat tccggcttg 
      gaagcgttg caagtgtta gacctgtct cataatctt 
      atcaaaacg tttgatgtc gagccggct tgtagtcgc 
      aatattgtt agtctaaaa ctgtcccat aacaaaatt 
      tacaatttt ccgcactgg atcatgacc tgtcattgc 
      cccaattta atcgaaatt gattacagt gccaatttt 
      ttgaccagc gtgagcgtg gcacgtaag ctaacgctc 
      aaaaaaatt acaatgaac aattcgcaa ttgcttgac 
      gttgatttc gagtttttc aaacgtata tcaacctta 
      gaatatcta gatataggt aacaccaat tgcaaatta 
      acaaaacag gagaacgaa tttaaagac ataactcaa 
      ctattcgtt aaaaccaaa tggaaagaa ctgaaagtt 
      ttgaagttg gaccacata cttgtgtct attatcccg 
      gacggactt ttctggatt gtgtcactg actgagctt 
      cacttaagt cattgcgag ctgagctgg ataccgcca 
      gaggtccag tatttaaca aacttggag gttctggat 
      gtgtctcat aattccatt tgcggaatt ccgaaagaa 
      ctagcgatt ttaccgaag ttgcgaatc gttaaagca 
      tcttttaat cttgtttcg tcagtggat gatttcacg 
      aatgagtta gaaattttg gatttgtat cataatgaa 
      cttgagacg tttccaaat tcaatcgag cgaattaaa 
      ttcgtcgat ttggagcag aattatgtc gatatgtcg 
      ctgtataaa acatatggc gaaaaacgg gattctttc 
      cgccaaatg cttgacgat gggagagct gacggtccg 
      agggttcca ccaacggag gttcccagc agtgacgat 
      gatcggctt tcatcagaa ggcagcgtt tgcggaaat 
      gatgatttt tatgacgct gatttggtt gatgaaacg 
      tgggattat gaggaacct aatgttaat gttggtcgt 
      gtgcggaac ccagaaata acgccctct gatgacgaa 
      tggacgggg agagaagaa ataatcatc agggaaaaa 
      agaacaacc aagccgtgg attgatgta aatgaagac 
      tggatattt tcagatgtt gaatgattg ttttgtatg 
      tttttaagt ttagaataa agtttttat tacaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY