Status
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Gene information
General information
TC007559 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3025794..3030235
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007559
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04347

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    abdomen segment number decreased # of remaining segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    pygopodium not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007559 RA
      MVKASTKAS TKTSTKTKK QKSAASLPE GQPGSLRSK 
      ISVLTEEDL TNSAEITIV IQSYQNIPA NYPSSHILT 
      KCQYMGKSI GESTKVLLT DDPILITDH KFIIRCNID 
      SPNGLDKLV THPVILQIF QTPNESRLF TESEHNLDI 
      EKIILDENL SSIESALSL YEALTGDVV EKVDIIASP 
      SNKKVKSKS KKKKSKDKI SKSSKGSKQ SKDNVKRKS 
      SKDSKKSKG SSKASSKKG RSKDKLPKK TDSVKSETE 
      TGACVIDLL PLIFGNSTF TEFVPLIIP HTFCDDRMT 
      RYSHKPGLS VMVSIDKPV EFPCTPILA NLTLESIYN 
      IPSIMNNEM ECQVCMVIP SANLPHKPV LFKNGTYKE 
      VAPQKEYKQ WPEVPNESY FNKTAYKVE SDTSNVKNC 
      VDNVKNIEE ATSESKPVI QFNCVKRNV LLKEGFFTL 
      YDHIRKYRR LVLEFYVFK KQTEHKKES KPSKVKSRT 
      SSTTIDEVK EKPTSYLHL MAVVDLSSL IFPGVTKLR 
      LAAPLTTFH YQEALFYGG LNDSHFFPS PKLESAVLH 
      KNESKLKAH KKDKKSKSD KSSAKSKGS KKTKGSKKS 
      KDSKKSKGS KKSKGSKKS KGSKKSKEK KAKKHPKED 
      LNLNDALPA EPPPETPPE PSKKVYNEK GQQTFIVLE 
      IELTQPLVE KATVSDLHG SLKQLVHVK PAVSKIMLN 
      KQLDTTLCK KIVKEVIYK INETYCTYI EKKNQCHCS 
      NQADFLTYI KRSGLYQNL VFTVGHVAC LIAQFKLDK 
      IEKVQSIHK YHNFLEHIF VWLMSEVNH VLNEMTTIG 
      RHIKAFPES SLKSLLFYA KEAEEIGNA ALAEQYYLK 
      RMQYDPTNP GPWFDFAVY KVGKGDYES ALMCLQEAL 
      TRKLYHHNS LLLTGVLLA ETKRFKEAE MCLLTLMVQ 
      ESRWTEGWA VLYLFYKEV DNAGGMDLS KEMVLKYLD 
      DPRSPTDDF IKNEDLVWT SEICPNTLY FKASVILLK 
      MRLYSWVEN ALACELREP GVVHYLLAV ICYYKKNYH 
      HALEHLDRA RFFHGSDRA VLSLTGHCY LAIGKQQEA 
      YEQYSHILE SFQRPDNIH LVYLNCAKV APNAQEAKK 
      LLLTACKHR PTPYTWMAC GIAYLKLND FLSAEECFS 
      ESVFLDNSR ADAWGYLTL TNLKMRRLN EAEMCYALA 
      LKNDLTDQC LLDEIQIGF EKLKSK
    CDS sequence
    • >TC007559 RA
      atggttaaa gcttcaact aaagcctct acaaaaact 
      tcgactaaa acaaaaaag caaaaaagt gccgctagt 
      cttccggaa ggtcagcct gggtcgctg aggtcaaaa 
      ataagcgtt ttgaccgaa gaagactta acgaattcg 
      gctgagatt acgatagtt atacaaagt taccagaat 
      attcctgcc aattatcct tcttcacat atattgacc 
      aaatgtcaa tatatgggg aaaagtatt ggggaaagt 
      acaaaagtg ctactcacc gatgatccg atcctaata 
      accgaccac aaatttatc attagatgt aacattgat 
      tcccccaat ggcttagat aaattagtg acccatccc 
      gtgatcctc caaattttt caaacacca aacgagtct 
      cgcttattc accgagagt gagcacaac ttggacatt 
      gaaaaaatt attttagac gaaaattta tcgtcaatt 
      gaaagcgct ctctcctta tacgaggca ctgaccgga 
      gacgtggtg gaaaaagtt gacattata gcctccccg 
      agtaataaa aaagtcaaa agcaaatcg aaaaaaaag 
      aaaagcaaa gacaaaatc agcaagtcg agtaaaggc 
      agcaagcaa agcaaagac aacgttaag cgaaaatca 
      agcaaagac agcaaaaaa tccaagggt tcgtcaaaa 
      gcatcatcg aaaaaaggg cgcagtaaa gacaaactc 
      ccgaaaaaa accgacagt gttaaaagc gaaacggaa 
      actggagct tgcgtcata gatctgttg ccgctcatt 
      tttggtaac tccactttt accgaattt gtgcccctg 
      atcatccct cacacgttt tgcgacgac cgtatgact 
      cggtattca cacaaacct gggctttct gtaatggta 
      tcaatcgat aaacctgta gaatttcct tgcacacca 
      attttagca aacttaacg ttggagtcg atttacaac 
      attccctca atcatgaat aacgaaatg gaatgccaa 
      gtttgtatg gtcatacct tctgcaaac ctgccccat 
      aaaccagtg ttgtttaaa aatggaact tacaaagaa 
      gtcgcgcca caaaaggag tacaaacag tggccggaa 
      gttccaaac gagagttat ttcaataaa acagcatat 
      aaagtggag agcgatact agcaatgtc aaaaactgt 
      gtagacaat gtcaagaac atagaggaa gccaccagt 
      gagagtaaa ccagtgata cagtttaac tgtgttaaa 
      agaaatgtc ctcctaaag gaagggttt ttcactttg 
      tatgaccac attcgaaaa tatcgcaga cttgtactt 
      gagttttat gtctttaag aagcaaact gaacataag 
      aaagaatca aaaccatcg aaagtcaaa agtaggaca 
      agttctaca actattgac gaagttaaa gaaaaacca 
      acgagttat cttcactta atggccgtg gtcgacctt 
      tcgtcgctc atttttccc ggagtcact aagctgcgt 
      ttggctgca cccttgacc acgtttcac taccaagaa 
      gccctgttt tacggtggt ttaaacgac tcacatttt 
      ttcccttct ccaaaactt gaatcggct gtattacat 
      aagaacgaa tctaagttg aaagctcac aaaaaagat 
      aaaaagtcc aagagcgac aaatcgagc gcgaaatca 
      aaaggaagt aagaaaaca aaagggagt aaaaaatct 
      aaagacagc aaaaaatcc aaagggagc aaaaaatcc 
      aaagggagc aaaaaatct aaagggagc aaaaaatca 
      aaagagaaa aaagccaag aaacacccg aaagaagat 
      ttaaattta aatgatgcg ttgccagcg gagccccct 
      cctgagaca cccccagaa cccagtaaa aaagtctac 
      aacgaaaag gggcaacaa acatttatt gtgttagaa 
      atagaactg acacaaccc ctagttgaa aaggctaca 
      gttagtgat ttacacggc agtttgaaa cagttagtt 
      catgtgaaa ccagcggtc tcaaaaata atgctcaac 
      aagcaactg gacacgaca ctgtgcaaa aaaattgtg 
      aaagaagtc atatataaa ataaacgaa acgtactgt 
      acttacatt gagaagaaa aatcagtgt cactgttca 
      aaccaagcc gactttttg acttacata aaacgttcc 
      ggtttgtac caaaacctg gtttttact gtgggacat 
      gtcgcctgt ttaatcgcc caatttaaa ttggacaag 
      atcgaaaaa gtgcagtcg attcacaaa tatcacaat 
      tttttggaa cacatattt gtctggttg atgagtgaa 
      gtcaatcat gttttgaat gaaatgacg acaataggc 
      cggcatatt aaggctttc cccgaaagt tcgctaaag 
      tctctctta ttttacgcc aaagaggcg gaagagatt 
      ggaaacgcg gctttggcg gaacaatat tatttaaag 
      agaatgcaa tacgaccca acaaaccct ggtccttgg 
      tttgatttt gctgtttac aaggtcgga aaaggggac 
      tacgaaagc gccctaatg tgtttacaa gaggcttta 
      accagaaaa ttgtaccat cacaatagt ttattacta 
      actggtgta cttctcgcc gaaactaaa cgtttcaaa 
      gaggcggaa atgtgttta ttgacttta atggttcaa 
      gagtctcgg tggactgaa ggatgggct gttctctac 
      ttattttac aaagaagtg gataacgct ggaggaatg 
      gatttatcg aaagaaatg gttttgaaa tatttggac 
      gatcctcga tctccaact gacgatttt ataaaaaat 
      gaggactta gtatggact tcggaaata tgtccgaac 
      acgttatat ttcaaagca agcgtcatt cttttgaaa 
      atgaggcta tactcatgg gtggagaac gcactagca 
      tgcgagttg agagagcct ggagttgtg cactatttg 
      ttggctgtt atttgttat tacaagaaa aattatcat 
      catgcactt gaacattta gaccgagca cgatttttc 
      catggttct gaccgtgca gttttgagt ttaacaggc 
      cactgttac ctagccatt gggaaacaa caagaagca 
      tacgaacag tattcacac attttagaa tcttttcaa 
      agacctgat aatattcat ctagtttac ttgaactgt 
      gctaaagta gcgcccaac gcacaggaa gcaaaaaaa 
      ttattactt acagcttgt aaacacaga ccgactcca 
      tacacatgg atggcttgt ggcattgca tatttgaaa 
      ttgaacgat tttttgagc gccgaggag tgtttttct 
      gagtcggtt ttcttagat aattcaaga gctgacgct 
      tggggttat ctaacattg actaatttg aaaatgcga 
      cggcttaat gaggctgaa atgtgttac gcattagca 
      ctgaagaat gatttaaca gatcaatgt ttgctggat 
      gagatacaa attgggttc gaaaaatta aaatcaaaa 
      taa
    mRNA sequence
    • >TC007559 RA
      ttaataact tgaaaatgg ttaaagctt caactaaag 
      cctctacaa aaacttcga ctaaaacaa aaaagcaaa 
      aaagtgccg ctagtcttc cggaaggtc agcctgggt 
      cgctgaggt caaaaataa gcgttttga ccgaagaag 
      acttaacga attcggctg agattacga tagttatac 
      aaagttacc agaatattc ctgccaatt atccttctt 
      cacatatat tgaccaaat gtcaatata tggggaaaa 
      gtattgggg aaagtacaa aagtgctac tcaccgatg 
      atccgatcc taataaccg accacaaat ttatcatta 
      gatgtaaca ttgattccc ccaatggct tagataaat 
      tagtgaccc atcccgtga tcctccaaa tttttcaaa 
      caccaaacg agtctcgct tattcaccg agagtgagc 
      acaacttgg acattgaaa aaattattt tagacgaaa 
      atttatcgt caattgaaa gcgctctct ccttatacg 
      aggcactga ccggagacg tggtggaaa aagttgaca 
      ttatagcct ccccgagta ataaaaaag tcaaaagca 
      aatcgaaaa aaaagaaaa gcaaagaca aaatcagca 
      agtcgagta aaggcagca agcaaagca aagacaacg 
      ttaagcgaa aatcaagca aagacagca aaaaatcca 
      agggttcgt caaaagcat catcgaaaa aagggcgca 
      gtaaagaca aactcccga aaaaaaccg acagtgtta 
      aaagcgaaa cggaaactg gagcttgcg tcatagatc 
      tgttgccgc tcatttttg gtaactcca cttttaccg 
      aatttgtgc ccctgatca tccctcaca cgttttgcg 
      acgaccgta tgactcggt attcacaca aacctgggc 
      tttctgtaa tggtatcaa tcgataaac ctgtagaat 
      ttccttgca caccaattt tagcaaact taacgttgg 
      agtcgattt acaacattc cctcaatca tgaataacg 
      aaatggaat gccaagttt gtatggtca taccttctg 
      caaacctgc cccataaac cagtgttgt ttaaaaatg 
      gaacttaca aagaagtcg cgccacaaa aggagtaca 
      aacagtggc cggaagttc caaacgaga gttatttca 
      ataaaacag catataaag tggagagcg atactagca 
      atgtcaaaa actgtgtag acaatgtca agaacatag 
      aggaagcca ccagtgaga gtaaaccag tgatacagt 
      ttaactgtg ttaaaagaa atgtcctcc taaaggaag 
      ggtttttca ctttgtatg accacattc gaaaatatc 
      gcagacttg tacttgagt tttatgtct ttaagaagc 
      aaactgaac ataagaaag aatcaaaac catcgaaag 
      tcaaaagta ggacaagtt ctacaacta ttgacgaag 
      ttaaagaaa aaccaacga gttatcttc acttaatgg 
      ccgtggtcg acctttcgt cgctcattt ttcccggag 
      tcactaagc tgcgtttgg ctgcaccct tgaccacgt 
      ttcactacc aagaagccc tgttttacg gtggtttaa 
      acgactcac attttttcc cttctccaa aacttgaat 
      cggctgtat tacataaga acgaatcta agttgaaag 
      ctcacaaaa aagataaaa agtccaaga gcgacaaat 
      cgagcgcga aatcaaaag gaagtaaga aaacaaaag 
      ggagtaaaa aatctaaag acagcaaaa aatccaaag 
      ggagcaaaa aatccaaag ggagcaaaa aatctaaag 
      ggagcaaaa aatcaaaag agaaaaaag ccaagaaac 
      acccgaaag aagatttaa atttaaatg atgcgttgc 
      cagcggagc cccctcctg agacacccc cagaaccca 
      gtaaaaaag tctacaacg aaaaggggc aacaaacat 
      ttattgtgt tagaaatag aactgacac aacccctag 
      ttgaaaagg ctacagtta gtgatttac acggcagtt 
      tgaaacagt tagttcatg tgaaaccag cggtctcaa 
      aaataatgc tcaacaagc aactggaca cgacactgt 
      gcaaaaaaa ttgtgaaag aagtcatat ataaaataa 
      acgaaacgt actgtactt acattgaga agaaaaatc 
      agtgtcact gttcaaacc aagccgact ttttgactt 
      acataaaac gttccggtt tgtaccaaa acctggttt 
      ttactgtgg gacatgtcg cctgtttaa tcgcccaat 
      ttaaattgg acaagatcg aaaaagtgc agtcgattc 
      acaaatatc acaattttt tggaacaca tatttgtct 
      ggttgatga gtgaagtca atcatgttt tgaatgaaa 
      tgacgacaa taggccggc atattaagg ctttccccg 
      aaagttcgc taaagtctc tcttatttt acgccaaag 
      aggcggaag agattggaa acgcggctt tggcggaac 
      aatattatt taaagagaa tgcaatacg acccaacaa 
      accctggtc cttggtttg attttgctg tttacaagg 
      tcggaaaag gggactacg aaagcgccc taatgtgtt 
      tacaagagg ctttaacca gaaaattgt accatcaca 
      atagtttat tactaactg gtgtacttc tcgccgaaa 
      ctaaacgtt tcaaagagg cggaaatgt gtttattga 
      ctttaatgg ttcaagagt ctcggtgga ctgaaggat 
      gggctgttc tctacttat tttacaaag aagtggata 
      acgctggag gaatggatt tatcgaaag aaatggttt 
      tgaaatatt tggacgatc ctcgatctc caactgacg 
      attttataa aaaatgagg acttagtat ggacttcgg 
      aaatatgtc cgaacacgt tatatttca aagcaagcg 
      tcattcttt tgaaaatga ggctatact catgggtgg 
      agaacgcac tagcatgcg agttgagag agcctggag 
      ttgtgcact atttgttgg ctgttattt gttattaca 
      agaaaaatt atcatcatg cacttgaac atttagacc 
      gagcacgat ttttccatg gttctgacc gtgcagttt 
      tgagtttaa caggccact gttacctag ccattggga 
      aacaacaag aagcatacg aacagtatt cacacattt 
      tagaatctt ttcaaagac ctgataata ttcatctag 
      tttacttga actgtgcta aagtagcgc ccaacgcac 
      aggaagcaa aaaaattat tacttacag cttgtaaac 
      acagaccga ctccataca catggatgg cttgtggca 
      ttgcatatt tgaaattga acgattttt tgagcgccg 
      aggagtgtt tttctgagt cggttttct tagataatt 
      caagagctg acgcttggg gttatctaa cattgacta 
      atttgaaaa tgcgacggc ttaatgagg ctgaaatgt 
      gttacgcat tagcactga agaatgatt taacagatc 
      aatgtttgc tggatgaga tacaaattg ggttcgaaa 
      aattaaaat caaaataag tcttcttta ttaatgttt 
      tgtattaaa aattataag taaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY