Status
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Gene information
General information
TC031587
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012975,TC012973,TC012972,TC012974
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450524.1: 4..20229
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031587
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02971

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_04345

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    iBeetle screen iB_04346

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    iBeetle screen iB_05363

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    iBeetle screen iB_06239

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031587 RA
      MGRHRYRGN VDSDDSDSS DDEPRPKRS TPGVSTTVN 
      SPPVSAESA RIARLEQLV ENLNRRLLN RNFEHTSGQ 
      VGIKSDLIP EFAPGNPNF STTKWLHKI DQLALVNGW 
      DERTTIYGM ISRLKGLAK EWYDNLDPS AYDRSWDEW 
      KRLLQATFP DHHDYASTL RKLVNRVKE DGETWAQYY 
      FGKLNLLQA CDITGTNAV SCLIDGITD VTLRNGARA 
      GRYVTPESL YAEYLSTLR SEGDKRDTL AKQAPRERR 
      RFLPSRVEA QKLYSSVRC YNCRNRGHV ATKCPKPRI 
      ECKKCGRIG HVDAECRRG NVVKENMSK QALTTSVAD 
      ASNNKNYYI DIKVNGKPT RAYIDSGAE PVLVKQSVA 
      RELGLMWQP SLHLIRGYG QGITKVHGE VEVELEVDL 
      VKANVKALI VEDSAQRVP VIVGQTFLN AGANTIIAN 
      EEAVRVVDG NNESIQAFL GQLPTRKVA LWAEEETVI 
      PPQSIGCIR IKAKDDGWK GAYYVEWCQ RPRPGFEHV 
      VHRCVTCDG GLVTVRNLS HQDLVYRKA QIVARAEPC 
      EQERTATTV SVFSVGKVE VKSFQRWEL LTQVNKNLD 
      PAQFEQLLQ LVNEFRDCF ARNVAEIGA TTAAEMKLT 
      LTDDKPVIY RPYRLSHHE RRIVRDIVN DLLGSGVIR 
      ESDSPYSSP ILLVRKKDG QHRMCVDYR QLNSKTIKD 
      RFPLPRVDE HLDKLKGAK FFTTLDLAS GYFQIPMAT 
      ESIPKTAFV TPDGHCEFV RMPFGLANA PAVFQRAMN 
      KVLGPLQFQ TAFCYIDDL LIPSKDFET GLNNLQTVF 
      QLLRQFGLT LKLSKYCFF GSQIEYLGH EISAEGIKP 
      GETKIKAVT AFPKPTDVH KLRQFLGLC GYFRKYVKD 
      YATIANSLT SLLKNGSAF VLEEVQERA FQTLKDILT 
      SRPVLAIYD AEAETELHT DASKVGIGG ILLQRQGDG 
      SLRPVMFFS RQTTKEEQR YHSYELETL AVVCSLKHY 
      RVYLLGLQF KVITDCNAL RTTLTKRDL IPRIGRWWL 
      LTSEFDFTV EYRPGSKMS HVDALSRNT VLDPSEPSV 
      EVLHINVND DDWILAVQM KDARCALLK EILEKSPTN 
      AEERAIHKE YKLKDGRVF KQTESGLKW VVPKAVRRQ 
      ILLAHHDGI GHLSNEKTL ASVSKSYWF PSMRSKKFK 
      THCTDLNVK LVLTATATP RANGQVERL NRTILSQLA 
      ASSREEEKW DDFVSRISW GINSTPNST TGKSPYELL 
      LGYTPRHAN DSILTNVVT EGVHDGDLP RTRADVAQR 
      VEKEQQKQK ERYDKRRCA PKRFNAGDL VLVRTTRVS 
      NEGKSRKLL PKYSGPYRI QQVLDYDRY VVTDMPGAT 
      RSQKRYEGV HSVDKLRHY VVATTSGDE TIGDELRNH 
      TLSIFIWVW LKRREKFSG EIFAMGRHR YRGNVDSDD 
      SDSSDDEPR PKRSAPGVS TTVNSPPVS AESARIARL 
      EQLVENLNR RLLNRNFEH TSGQVGIKS DLIPEFAPG 
      NPNFSTTKW LHKIDQLAL VNGWDERTT IYGMISRLK 
      GLAKEWYDN LDPSAYDRS WDEWKRLLQ ATFPDHHDY 
      ASTLLSCLI DGMTDVTLR NGARAGRYV TPESLYAEY 
      LSTLRSEGD KRDTLAKQA PRERRRFLP SRVEAQKLY 
      SSVRCYNCR NRGHVATKC PKPRIECKK CGRIGHVDA 
      ECRRGNVVK ENMSKQALT TSVADASNN KNYYIDIKV 
      NGKPTRAYI DSGAEPVLV KQSVARELG LMWQPSLHL 
      IRGYGQGIT KVHGEVEVE LEVDLVKAN VKALIVEDS 
      AQRVPVIVG QTFLNAGAN TIIANEEAV RVVDGNNES 
      IQAFLGQLP TRKVALWAE EETVIPPQS IGCIRIKAK 
      DDGWKGAYY VEGCQRPRP GFEHVVHRC VTCDGGLVT 
      VRNLSHQDL VYRKAQIVA RAEPCEQER TATTVSVFS 
      VGKVEVKSF QRWELLTQV NKNLDPAQF EQLLQLVNE 
      FRDCFARNV AEIGATTVA EMKLTLTDD KPVIYRPYR 
      LSHHERRIV RDIVNDLLG SGVIRESDS PYSSPILLV 
      RKKDGQHRM CVDYRQLNS KTIKDRFPL PRVDEHLDK 
      LNGAKFFTT LDLASGYYQ IPMATESIP KTAFVTPDG 
      HYEFVRMPF GLANAPAVF QRAMNKVLG PLRFQTAFC 
      YIDDLLIPS KDFETGLNN LRTVFQLLR QFGLTLKLS 
      KCCFFGSQI EYLGHEISA EGIKPGETK IKAVTAFPK 
      PTDVHKLRQ FLGLCGYFR KYVKDYATI ANSLTSLLK 
      KGSAFVWEE AQERAFQTL KDILTSRPV LAIYDAEAE 
      TELHTDASK VGIGGILLQ RQGDGSLRP VMFFSRQTT 
      KEEQRYHSY ELETLAVVC SLKHYRVYL LGLQFKVIT 
      DCNALRTTL TKRDLIPRI GRWWLLTSE FDFTVEYRP 
      GSKMSHVDA LSRNPVLDP SEPSVEVLH INVNDDDWI 
      LAVQMKDAR CALLKEILE KSPTNAEER AIHKEYKLK 
      DGRVFKQTE SGLKWVVPK AVRRQILLA HHDGIGHLS 
      NEKTLASVS KSYWFPSMR SKKFKTHCT DLNVKLVLT 
      ATATPRANG QVERLNRTI LSQLAASSR EEEKWDDFV 
      SRISWGINS TPNSTTGKS PYELLLGYT PRHANDSIL 
      TNVVTEGVH DGDLPRTRA DVAQRVEKE QQKQKERYD 
      KRRCAPKRF NAGDLVLVR TTRASNEGK SRKLLPKYS 
      GPYRIQKVL DYDRYRGNV DSDDSDSSD DEPRPKRSA 
      PGVSTTVNS PPVSAESAR IARLEQLVE NLNRRLLNR 
      NFEHTSGQV GIKSDLIPE FAPGNPNFS TTKWLHKID 
      QLALVNGWD ERTTIYGMI SRLKGLAKE WYDNLDPSA 
      YDRSWDEWK LSCLIDGMT DVTLRNGAR AGRYVTPES 
      LYAEYLSTL RSEGDKRDT LAKQAPRER RRFLPSRVE 
      TQKLYSARN QELSARNVV ALDMWMLSR RGNLVKENM 
      SKQALTTSV ADASNNKNY YIDIKVNGK PTRAYIDSG 
      AEPVLVKQS VARELGLMW QPSLHLIRG YGQGITKVH 
      GEVEVELEV DLVKANVKA LIVEDSAQR VPVIVGQTF 
      LNAGANTII ANEEAVRVV DGNNESIQA FLGQLPTRK 
      VALWAEEET VIPPQSIGC IRIKAKDDG KKFKTHCTD 
      LNVKLVLTA TATPRANGQ VERLNRTIL SQLAASSRE 
      EEKWDDFVS RISWGINST PNSTTGKSP YELLLGYTP 
      RHANDSILT NVVTEGVHD GDLPRTRAD VAQRVEKEQ 
      QKQKERYDK RRCAPKRFN AGDLVLVRT TRASNEGKS 
      RKLLPKYSG PYRIQKVLD YDRYVVTDM PGATRSQKR 
      YEGVHSVDK LRHYVVATT SGDETIGDE LRNHTLSIF 
      IWVWLKRRE KFSGEIFAM GRHRYRGNV DSDDSDSSD 
      DEPRPKRSA PGVSTTVNS PPVSAESAR IARLEQLVE 
      NLNRRLLNR NFEHTSGQV GIKSGLIPE FAPGNPNFS 
      TTKWLHKID QLALVNGWD ERTTIYGMI SRLKGLAKE 
      WYDNLDPSA YDRSWDEWK RLLQATFPD HHDYASTLR 
      KLVNRVKED GETWAQYYF GKLNLLQAC DITGTNAVS 
      CLIDGITDV TLRNGARAG RYVTPESLY AEYLSTLRS 
      EGDKRDTLA KQAPRERRR FLPSRVEAQ KLYSSVRCY 
      NCRNRGHVA TKCPKPRIE CKKCGRIGH VDAECRRGN 
      VVKENMSKQ ALTTSVADA SNNKNYYID IKVNGKPTR 
      AYIDSGAEP VLVKQSVAR ELGLMWQPS LHLIRGYGQ 
      GITKVHGEV EVELEVDLV KANVKALIV EDSAQRVPV 
      IVGQTFLNA GANTIIANE EAVRVVDGN NESIQAFLG 
      QLPTRKVAL WAEDETVIP PQSIGCIRI KAKDDGWKG 
      AYYVEGCQR PRPGFEHVV HRCVTCDGG LVTVRNLSH 
      QDLVYRKAQ IVARAEPCE QEWTATTLA ASSREEEKW 
      DDFVSRISW GINSTPNST TGKSPYELL LGYTPRHAN 
      DSILTNVVT EGVHDGDLP RTRADVAQR VEKEQQKQK 
      ERYDKRRCA PKRFNAGDL VLVRTTRAS NEGKSRKLL 
      PKYSGPYRI QKVLDYDRY VVTDMPGAT RSQKRYEGV 
      HSVDKLRHY VVATTSGDE TIGDVSDE
    CDS sequence
    • >TC031587 RA
      atggggcgt cacaggtat cgaggaaat gtggactcg 
      gacgactcg gactcaagt gacgatgag ccacggccc 
      aaaaggtcc acgcctggg gtctccact acagtaaat 
      tcaccccca gtttcggcc gagtccgct agaatcgcg 
      agacttgag caacttgtg gaaaacttg aatcgacgt 
      ttgctaaac cgcaatttt gagcacaca tcaggacaa 
      gttggcatt aaaagtgac ctgattcct gaattcgct 
      ccaggaaac cccaatttt agtaccact aagtggttg 
      cataaaatt gaccagttg gctctcgtg aacggttgg 
      gacgaacgt acaacgatt tatggcatg ataagtcga 
      ctcaagggg ttggccaag gagtggtac gacaatctc 
      gatccctca gcatacgac cggagctgg gatgaatgg 
      aaacgtttg ttacaagcc accttcccc gatcaccac 
      gattatgct tccacactt cgtaagcta gtgaatagg 
      gtgaaggaa gacggtgaa acctgggca caatattat 
      tttgggaaa ttaaatctg ttgcaggct tgtgacata 
      acaggaacg aatgcagtc tcctgtctt attgatgga 
      atcactgat gtgactctc agaaatggg gctagagca 
      gggcgttac gttacaccc gaaagcttg tacgctgag 
      tatttgtcg actctgaga tccgaaggt gacaagcgg 
      gatacgctc gcaaagcag gcgcctcgg gaaaggagg 
      aggttcctt ccaagtcga gtcgaagca caaaaactg 
      tactcgtca gttcggtgt tacaattgt cgtaatcga 
      ggacatgtt gcgacaaag tgcccgaaa ccaagaatt 
      gagtgcaag aaatgtggt cgcattgga catgtggat 
      gctgagtgc cgacgtggt aacgtagta aaagaaaac 
      atgtctaag caggcacta acgacaagt gtagctgac 
      gcaagtaat aataagaat tattacata gacattaag 
      gtcaatggt aagccgacg cgagcttac attgactca 
      ggagctgaa ccggtcctt gtcaagcaa agtgttgct 
      agagaattg ggactgatg tggcagcca agtttacat 
      ttgattcgt ggttatggc cagggaatc accaaagtg 
      catggggaa gtcgaagta gagctagaa gtggatctt 
      gtcaaggca aacgtaaaa gctttgatc gtcgaagac 
      agtgcacaa cgtgtgcct gtcatagtg ggacagacc 
      ttcctgaat gcgggtgct aatacgatt atagcaaat 
      gaggaagct gtgagagta gttgacgga aacaacgaa 
      tcgatccaa gcatttcta ggccaactt ccgacacga 
      aaagttgca ctttgggcg gaagaggaa acagtgatc 
      cctcctcaa tccattggt tgcatacga atcaaggca 
      aaagacgat gggtggaaa ggagcttat tacgttgaa 
      tggtgtcaa cgtccgaga ccagggttc gaacacgtt 
      gttcatcgg tgtgtcaca tgtgatgga ggcctagtg 
      accgttcgc aatctatcg caccaagat ctggtctat 
      cgaaaggcg caaatcgtt gcgagagct gagccttgc 
      gagcaggaa aggacagct acgacggtg agtgtgttt 
      tctgtgggg aaggttgag gtaaagagt tttcaacgg 
      tgggaactt cttacacaa gtcaataag aatctagac 
      ccggctcag ttcgagcaa ttgttacaa ctcgttaac 
      gaatttcga gattgtttt gcacgaaat gtagcggaa 
      ataggtgcc acaactgcg gcagaaatg aagctaact 
      ttgactgat gataaacct gtaatttat cgtccttat 
      cggttatcg catcacgaa agaagaatt gttcgagat 
      atagtaaat gacttgtta ggtagcgga gtcatacga 
      gaatcagac tcgccatat tcgagtccc atactgttg 
      gtgcggaag aaagatggg caacaccgt atgtgtgtt 
      gactatcga caattaaat tcgaaaaca atcaaagat 
      cgctttccg ttaccacga gtagatgaa catttggat 
      aaactaaaa ggtgcaaag tttttcacc acactagat 
      ctcgcgagt gggtacttt cagatccca atggccacc 
      gaatcgatt ccaaagaca gcatttgtt acgcctgat 
      gggcattgc gagtttgtc cgcatgcct tttggtcta 
      gccaacgct ccggccgtg tttcagcga gctatgaat 
      aaggtgttg ggtccatta cagtttcag accgctttt 
      tgttacata gatgatctt ctgatacct tccaaagac 
      tttgaaaca ggtcttaac aatttacaa acggtgttt 
      caattgctt cgacagttc ggattgact ctaaagctg 
      agtaaatat tgcttcttt ggaagtcaa atagagtat 
      ttagggcat gagatcagt gcggaaggc attaagcca 
      ggcgagaca aaaatcaaa gcggtgaca gcttttccc 
      aaaccaaca gacgtacac aaacttagg caattctta 
      ggtttgtgt gggtacttt cgaaagtac gtgaaagat 
      tacgcaaca atagcaaac agtttgaca agtctccta 
      aagaatggc agtgcattt gttttggaa gaagtacaa 
      gaacgggct tttcagact ttaaaagac atcttgacg 
      agcagacca gttcttgca atttacgac gcggaagct 
      gaaacggag ttacacact gacgcttct aaagttgga 
      attggtggc attctgttg caacgacaa ggtgatgga 
      tctttgcgt ccagttatg tttttcagc cgacagacg 
      accaaagag gaacaaagg taccactcg tacgagcta 
      gaaacgcta gcggtggtc tgttctctc aaacattat 
      cgagtatac ttgttgggg cttcaattc aaggtgatc 
      acagactgt aatgcttta cgaacaacc cttaccaag 
      agagatttg attccacga attgggaga tggtggttg 
      ttgacttct gagttcgac tttactgta gaatacaga 
      cctggcagt aaaatgagt cacgttgac gctttgagt 
      agaaatacg gtattagat ccttcggag ccttcagtg 
      gaagttttg catattaat gttaacgat gatgattgg 
      attttagct gttcaaatg aaggatgcg agatgcgca 
      ttgttgaag gaaatattg gaaaaatca ccgactaac 
      gctgaagag cgtgccatt cacaaagag tataaacta 
      aaagatggt cgagtattc aaacagact gagtcagga 
      ttaaaatgg gtcgtacca aaagcggta agacgccag 
      attttatta gcacaccac gatggaatt ggccattta 
      tcgaatgag aaaactttg gcatctgtg tcgaaatct 
      tattggttt ccttcaatg cggagcaaa aagtttaag 
      acacattgt accgattta aacgttaaa ttagtacta 
      accgcgact gccacacca cgggctaat ggccaggtg 
      gagcgatta aatcgtacg attttatct cagcttgct 
      gctagctcg agagaagaa gagaaatgg gatgacttt 
      gtgagtaga attagttgg ggtattaat tctacgccg 
      aattcgact acgggcaaa agtccatac gaattgttg 
      ttggggtat acacctcga catgctaat gactcaatc 
      ctcaccaac gtggtgaca gagggtgtt catgatggc 
      gatttgcca cggacacga gcagacgtt gctcagcgt 
      gtcgaaaag gaacagcag aagcaaaag gagcgttat 
      gacaagcga cgctgtgcc ccaaagaga tttaatgca 
      ggtgatctc gtgttggta cgcaccacg cgagtttca 
      aacgagggc aaaagtcgg aagctgtta cctaaatat 
      tcgggaccg taccgaatt cagcaagtg ctcgactac 
      gataggtat gtcgtgacg gacatgccg ggggcgacg 
      cgttcccaa aaacgatat gagggtgtt cactcagtg 
      gacaaattg agacattac gttgtcgcc acgacgagt 
      ggagacgaa acaataggc gatgagtta cgcaaccac 
      acgctgtca attttcatt tgggtgtgg ctcaaaagg 
      cgcgaaaag ttttctggt gagattttc gcaatgggg 
      cgtcacagg taccgagga aatgtggac tcggacgac 
      tcggactca agtgacgat gagccacgg ccgaaaagg 
      tccgcgcct ggggtctcc actacagta aattcaccc 
      ccagtttcg gccgagtcc gctagaatc gcgagactt 
      gagcaactt gtggaaaac ttgaatcga cgtttgcta 
      aaccgcaat tttgagcac acatcagga caagttggc 
      attaaaagt gacctgatt cctgaattc gctccagga 
      aaccccaat tttagtacc actaagtgg ttgcataaa 
      attgaccag ttggctctc gtgaacggt tgggacgaa 
      cgtacaacg atttacggc atgataagt cgactcaag 
      gggttggcc aaggagtgg tacgacaat ctcgatccc 
      tcagcatac gaccggagc tgggatgaa tggaaacgt 
      ttgttacaa gccaccttc cccgatcac cacgattat 
      gcttccaca cttctctcc tgtcttatt gatggaatg 
      actgacgtg actctcaga aatggggct agagcaggg 
      cgttacgtt acacccgaa agcttgtac gctgagtat 
      ttgtcgact ctgagatcc gaaggtgac aagcgggat 
      acgctcgca aagcaggcg cctcgggaa aggaggagg 
      ttccttcca agtcgagtt gaagcacaa aaactgtat 
      tcgtcagtt cggtgttac aattgtcgt aatcgagga 
      catgttgcg acaaagtgc ccgaaacca agaattgag 
      tgcaagaaa tgtggtcgc attggacat gtggatgct 
      gagtgccga cgtggtaac gtagtaaaa gaaaacatg 
      tctaagcag gcactaacg acaagtgta gctgacgca 
      agtaataat aagaattat tacatagac attaaggtc 
      aatggtaag ccgacgcga gcttacatt gactcagga 
      gctgaaccg gtcctggtc aagcaaagt gttgctaga 
      gaattggga ctgatgtgg cagccaagt ttacatttg 
      attcgtggt tatggccag ggaatcacc aaagtgcat 
      ggggaagtc gaagtagag ctagaagtg gatcttgtc 
      aaggcaaac gtaaaagct ttgatcgtc gaagacagt 
      gcacaacgt gtgcctgtc atagtggga cagaccttc 
      ctgaatgcg ggtgctaat acgattata gcaaatgag 
      gaagctgtg agagtagtt gacggaaac aacgaatcg 
      atccaagca tttctaggc caacttccg acacgaaaa 
      gttgcactt tgggcggaa gaggaaaca gtgatccct 
      cctcaatcc attggttgc atacgaatc aaggcaaaa 
      gacgatggg tggaaagga gcttattac gttgaaggg 
      tgtcaacgt ccgagacca gggttcgaa cacgttgtt 
      catcggtgt gtcacatgt gatggaggc ctagtgacc 
      gttcgcaat ctatcgcac caagatctg gtctatcga 
      aaggcgcaa atcgttgcg agagctgag ccttgcgag 
      caggaaagg acagctacg acggtgagt gtgttttct 
      gtggggaag gttgaggta aagagtttt caacggtgg 
      gaacttctt acacaagtc aataagaat ctagacccg 
      gctcagttc gagcaattg ttacaactc gttaacgaa 
      tttcgagat tgttttgca cgaaatgta gcggaaata 
      ggtgccaca actgtggca gaaatgaag ctaactttg 
      actgatgat aaacctgta atttatcgt ccttatcgg 
      ttatcgcat cacgaaaga agaattgtt cgagatata 
      gtaaatgac ttgttaggt agcggagtc atacgagaa 
      tcagactcg ccatattcg agtcccata ctgttggtg 
      cggaagaaa gatgggcaa caccgtatg tgtgttgac 
      tatcggcaa ttaaattcg aaaacaatc aaagatcgc 
      tttccgtta ccacgagta gatgaacat ttggataaa 
      ctaaacggt gcaaagttt ttcaccaca ctagatctc 
      gcgagtggg tactatcag atcccaatg gccaccgaa 
      tcgattcca aagacagca tttgttacg cctgatggg 
      cattacgag tttgtccgc atgcctttt ggtctagcc 
      aacgctccg gcagtgttt cagcgagct atgaataag 
      gtgttgggt ccattacgg tttcagacc gctttttgt 
      tacatagat gatcttctg ataccttcc aaagacttt 
      gaaacaggt cttaacaat ttacgaacg gtgtttcaa 
      ttgcttcga cagttcgga ttgactcta aagctgagt 
      aaatgttgc ttctttgga agtcaaata gagtattta 
      gggcatgag atcagtgcg gaaggcatt aagccaggc 
      gagacaaaa atcaaagcg gtgacagct tttcccaaa 
      ccaacagac gtacacaaa cttaggcaa ttcttaggt 
      ttgtgtggg tactttcga aagtacgtg aaagattac 
      gcaacaata gcaaacagt ttgacgagt ctcctaaag 
      aaaggcagt gcatttgtt tgggaagaa gcacaagag 
      cgggctttt cagacttta aaagacatc ttgacgagc 
      agaccagtt cttgcaatt tacgacgcg gaagctgaa 
      acggagtta cacactgac gcttctaaa gttggaatt 
      ggtggcatt ctgttgcaa cgacaaggt gatggatct 
      ttgcgtcca gttatgttt ttcagccga cagacgacc 
      aaagaggaa caaaggtac cactcgtac gagctagaa 
      acgctagcg gtggtctgt tctctcaaa cattatcga 
      gtatacttg ttggggctt caattcaag gtgatcaca 
      gactgtaat gctttacga acaaccctt accaagaga 
      gatttgatt ccacgaatt gggagatgg tggttgttg 
      acttctgag ttcgacttt actgtagaa tacagacct 
      ggcagtaaa atgagtcac gttgacgct ttgagtaga 
      aatccggta ttagatcct tcggagcct tcagtggaa 
      gtcttgcat attaatgtt aacgatgat gattggatt 
      ttagctgtt caaatgaag gatgcgaga tgcgcattg 
      ttgaaggaa atattggaa aaatcaccg acgaacgct 
      gaagagcgt gccattcac aaagagtat aaactaaaa 
      gatggtcga gtattcaaa cagactgag tcaggatta 
      aaatgggtc gtaccaaaa gcggtaaga cgccagatt 
      ttattagca caccacgat ggaattggc catttatcg 
      aatgagaaa actttggca tctgtgtcg aaatcttat 
      tggtttcct tcaatgcgg agcaaaaag tttaagaca 
      cattgtacc gatttaaac gttaaatta gtactaacc 
      gcgactgct acaccacgg gctaacggc caggtggag 
      cgattgaat cgtacgatt ttatctcag cttgctgct 
      agctcgaga gaagaagag aaatgggat gactttgtg 
      agtagaatt agttggggt attaattct acgccgaat 
      tcgactacg ggcaaaagt ccatacgaa ttgttgttg 
      gggtataca cctcgacat gctaatgac tcaatcctc 
      accaacgtg gtgacagag ggtgttcat gatggtgat 
      ttgccacgg acacgagca gacgttgct cagcgtgtc 
      gaaaaggaa cagcagaag caaaaagag cgttatgac 
      aagcgacgc tgtgcccca aagagattt aatgcaggt 
      gatctcgtg ttggtacgc accacgcga gcttcaaac 
      gagggcaaa agtcggaag ctgttacct aaatattcg 
      ggaccgtac cgaattcag aaagtgctc gactacgat 
      aggtaccga ggaaatgtg gactcggac gactcggac 
      tcaagtgac gatgagcca cggccgaaa aggtccgcg 
      cctggggtc tccactaca gtaaattca cccccagtt 
      tcggccgag tccgctaga atcgcgaga cttgagcaa 
      cttgtggaa aacttgaat cgacgtttg ctaaaccgt 
      aattttgag cacacatca ggacaagtt ggcattaaa 
      agtgacttg attcctgaa ttcgctcca ggaaacccc 
      aattttagt accactaag tggttgcat aaaattgac 
      cagttggct ctcgtgaac ggttgggac gaacgtaca 
      acgatttat ggcatgata agtcgactc aaggggttg 
      gccaaggag tggtacgac aatctcgat ccctcagca 
      tatgaccgg agctgggat gaatggaaa ctctcctgt 
      cttattgat ggaatgact gacgtgact ctcagaaat 
      ggggctaga gcagggcgt tacgttaca cccgaaagc 
      ttgtacgct gagtatttg tcgactctg agatccgaa 
      ggtgacaag cgggatacg ctcgcaaag caggcgcct 
      cgggaaagg aggaggttc cttccaagt cgagtcgaa 
      acacaaaaa ctgtattct gcccgaaac caagaattg 
      agtgcaaga aatgtggtc gcattggac atgtggatg 
      cttagccga cgtggtaac ttagtaaaa gaaaacatg 
      tctaagcag gcactaacg acaagtgta gctgacgca 
      agtaataat aagaattat tacatagac attaaggtc 
      aatggcaag ccgacgcga gcttacatt gactcagga 
      gctgaaccg gtccttgtc aagcaaagt gttgctaga 
      gaattggga ctgatgtgg cagccaagt ttacatttg 
      attcgtggt tatggccag ggaatcacc aaagtgcat 
      ggggaagtc gaagtagag ctagaagtg gatcttgtc 
      aaggcaaac gtaaaagct ttgatcgtc gaagacagt 
      gcacaacgt gtgcctgtc atagtggga cagaccttc 
      ctgaatgcg ggtgctaat acgattata gcaaatgag 
      gaagctgtg agagtagtt gacggaaac aacgaatcg 
      atccaagca tttctaggc caacttccg acacgaaaa 
      gttgcactt tgggcggaa gaggaaaca gtgatccct 
      cctcaatcc attggttgc atacgaatc aaggcaaaa 
      gacgatggc aaaaagttt aagacacat tgtaccgat 
      ttaaacgtt aaattagta ctaaccgcg actgctaca 
      ccacgggct aacggccag gtggagcga ttgaatcgt 
      acgatttta tctcagctt gctgctagc tcgagagaa 
      gaagagaaa tgggatgac tttgtgagt agaattagt 
      tggggtatt aattctacg ccgaattcg actacgggc 
      aaaagtcca tacgaattg ttgttgggg tatacacct 
      cgacatgct aatgactca atcctcacc aacgtggtg 
      acagagggt gttcatgat ggtgatttg ccacggaca 
      cgagcagac gttgctcag cgtgtcgaa aaggaacag 
      cagaagcaa aaagagcgt tatgacaag cgacgctgt 
      gccccaaag agatttaat gcaggtgat ctcgtgttg 
      gtacgcacc acgcgagct tcaaacgag ggcaaaagt 
      cggaagctg ttacctaaa tattcggga ccgtaccga 
      attcagaaa gtgctcgac tacgatagg tacgtcgtg 
      acggacatg ccgggggcg acgcgttcc caaaaacga 
      tatgagggt gttcactca gtggacaaa ttgagacat 
      tacgttgtc gccacgacg agtggagac gaaacaata 
      ggcgatgag ttacgcaac cacacgctg tcaattttc 
      atttgggtg tggctcaaa aggcgcgaa aagttttct 
      ggtgagatt ttcgcaatg gggcgtcac aggtaccga 
      ggaaatgtg gactcggac gactcggac tcaagtgac 
      gatgagcca cggccgaaa aggtccgcg cctggggtc 
      tccactaca gtaaattca cccccagtt tcggccgag 
      tccgctaga atcgcgaga cttgagcaa cttgtggaa 
      aacttgaat cgacgtttg ctaaaccgc aattttgag 
      cacacatca ggacaagtt ggcattaaa agtggcctg 
      attcctgaa ttcgctcca ggaaacccc aattttagt 
      accactaag tggttgcat aaaattgac cagttggct 
      ctcgtgaac ggttgggac gaacgtaca acgatttac 
      ggcatgata agtcgactc aaggggttg gccaaggag 
      tggtacgac aatctcgat ccctcagca tacgaccgg 
      agctgggat gaatggaaa cgtttgtta caagccacc 
      ttccccgat caccacgat tatgcttcc acacttcgt 
      aagctagtg aatagggtg aaggaagat ggggaaacc 
      tgggcacaa tattatttt gggaaatta aatctgttg 
      caggcttgt gacataaca ggaacgaat gcagtctcc 
      tgtcttatt gatggaatc actgacgtg actctcaga 
      aatggggct agagcaggg cgttacgtt acacccgaa 
      agcttgtac gctgagtat ttgtcgact ctgagatcc 
      gaaggtgac aagcgggat acgctcgca aagcaggcg 
      cctcgggaa aggaggagg ttccttcca agtcgagtc 
      gaagcacaa aaactgtat tcgtcagtt cggtgttac 
      aattgtcgt aatcgagga catgttgcg acaaagtgc 
      ccgaaacca agaattgag tgcaagaaa tgtggtcgc 
      attggacat gtggatgct gagtgccga cgtggtaac 
      gtagtaaaa gaaaacatg tctaagcag gcactaacg 
      acaagtgta gctgacgca agtaataat aagaattat 
      tacatagac attaaggtt aatggtaag ccgacgcga 
      gcttacatt gactcagga gctgaaccg gtccttgtc 
      aagcaaagt gttgctaga gaattggga ctgatgtgg 
      cagccaagt ttacatttg attcgtggt tatggccag 
      ggaatcacc aaagtgcat ggggaagtc gaagtagag 
      ctagaagtg gatcttgtc aaggcaaac gtaaaagct 
      ttgatcgtc gaagacagt gcacaacgt gtgcctgtc 
      atagtggga cagaccttc ctgaatgcg ggtgctaat 
      acgattata gcaaatgaa gaagctgtg agagtagtt 
      gacggaaac aacgaatcg atccaagca tttctaggc 
      caacttccg acacgaaaa gttgcactt tgggcggaa 
      gacgaaaca gtgatccct cctcaatcc attggttgc 
      atacgaatc aaggcaaaa gacgatggg tggaaagga 
      gcttattac gttgaaggg tgtcaacgt ccgagacca 
      gggttcgaa cacgttgtt catcggtgt gtcacatgt 
      gatggaggc ctagtgacc gttcgcaat ctatcgcac 
      caagatctg gtctatcga aaggcgcaa atcgttgcg 
      agagctgag ccttgcgag caggaatgg acagctacg 
      acgcttgct gctagctcg agagaagaa gagaaatgg 
      gatgacttt gtgagtaga attagttgg ggtattaat 
      tctacgccg aattcgact acgggcaaa agtccatac 
      gaattgttg ttggggtat acacctcga catgctaat 
      gactcaatc ctcaccaac gtggtgaca gagggtgtt 
      catgatggt gatttgcca cggacacga gcagacgtt 
      gctcagcgt gtcgaaaag gaacagcag aagcaaaaa 
      gagcgttat gacaagcga cgctgtgcc ccaaagaga 
      tttaatgca ggtgatctc gtgttggta cgcaccacg 
      cgagcttca aacgagggc aaaagtcgg aagctgtta 
      cctaaatat tcgggaccg taccgaatt cagaaagtg 
      ctcgactac gataggtac gtcgtgacg gacatgccg 
      ggggcgacg cgttcccaa aaacgatat gagggtgtt 
      cactcagtg gacaaattg agacattac gttgtcgcc 
      acgacgagt ggagacgaa acaataggc gatgtcagt 
      gacgaatga
    mRNA sequence
    • >TC031587 RA
      agattttta aagttatta taaatccaa acgactgtg 
      atatattac aactgtaga tttaaatag tttttgagt 
      tacgcaacc acacgctgt caattttca tttgggtgt 
      ggctcaaaa ggcgcgaaa agttttcta atgagattt 
      tcgcaatgg ggcgtcaca ggtatcgag gaaatgtgg 
      actcggacg actcggact caagtgacg atgagccac 
      ggcccaaaa ggtccacgc ctggggtct ccactacag 
      taaattcac ccccagttt cggccgagt ccgctagaa 
      tcgcgagac ttgagcaac ttgtggaaa acttgaatc 
      gacgtttgc taaaccgca attttgagc acacatcag 
      gacaagttg gcattaaaa gtgacctga ttcctgaat 
      tcgctccag gaaacccca attttagta ccactaagt 
      ggttgcata aaattgacc agttggctc tcgtgaacg 
      gttgggacg aacgtacaa cgatttatg gcatgataa 
      gtcgactca aggggttgg ccaaggagt ggtacgaca 
      atctcgatc cctcagcat acgaccgga gctgggatg 
      aatggaaac gtttgttac aagccacct tccccgatc 
      accacgatt atgcttcca cacttcgta agctagtga 
      atagggtga aggaagacg gtgaaacct gggcacaat 
      attattttg ggaaattaa atctgttgc aggcttgtg 
      acataacag gaacgaatg cagtctcct gtcttattg 
      atggaatca ctgatgtga ctctcagaa atggggcta 
      gagcagggc gttacgtta cacccgaaa gcttgtacg 
      ctgagtatt tgtcgactc tgagatccg aaggtgaca 
      agcgggata cgctcgcaa agcaggcgc ctcgggaaa 
      ggaggaggt tccttccaa gtcgagtcg aagcacaaa 
      aactgtact cgtcagttc ggtgttaca attgtcgta 
      atcgaggac atgttgcga caaagtgcc cgaaaccaa 
      gaattgagt gcaagaaat gtggtcgca ttggacatg 
      tggatgctg agtgccgac gtggtaacg tagtaaaag 
      aaaacatgt ctaagcagg cactaacga caagtgtag 
      ctgacgcaa gtaataata agaattatt acatagaca 
      ttaaggtca atggtaagc cgacgcgag cttacattg 
      actcaggag ctgaaccgg tccttgtca agcaaagtg 
      ttgctagag aattgggac tgatgtggc agccaagtt 
      tacatttga ttcgtggtt atggccagg gaatcacca 
      aagtgcatg gggaagtcg aagtagagc tagaagtgg 
      atcttgtca aggcaaacg taaaagctt tgatcgtcg 
      aagacagtg cacaacgtg tgcctgtca tagtgggac 
      agaccttcc tgaatgcgg gtgctaata cgattatag 
      caaatgagg aagctgtga gagtagttg acggaaaca 
      acgaatcga tccaagcat ttctaggcc aacttccga 
      cacgaaaag ttgcacttt gggcggaag aggaaacag 
      tgatccctc ctcaatcca ttggttgca tacgaatca 
      aggcaaaag acgatgggt ggaaaggag cttattacg 
      ttgaatggt gtcaacgtc cgagaccag ggttcgaac 
      acgttgttc atcggtgtg tcacatgtg atggaggcc 
      tagtgaccg ttcgcaatc tatcgcacc aagatctgg 
      tctatcgaa aggcgcaaa tcgttgcga gagctgagc 
      cttgcgagc aggaaagga cagctacga cggtgagtg 
      tgttttctg tggggaagg ttgaggtaa agagttttc 
      aacggtggg aacttctta cacaagtca ataagaatc 
      tagacccgg ctcagttcg agcaattgt tacaactcg 
      ttaacgaat ttcgagatt gttttgcac gaaatgtag 
      cggaaatag gtgccacaa ctgcggcag aaatgaagc 
      taactttga ctgatgata aacctgtaa tttatcgtc 
      cttatcggt tatcgcatc acgaaagaa gaattgttc 
      gagatatag taaatgact tgttaggta gcggagtca 
      tacgagaat cagactcgc catattcga gtcccatac 
      tgttggtgc ggaagaaag atgggcaac accgtatgt 
      gtgttgact atcgacaat taaattcga aaacaatca 
      aagatcgct ttccgttac cacgagtag atgaacatt 
      tggataaac taaaaggtg caaagtttt tcaccacac 
      tagatctcg cgagtgggt actttcaga tcccaatgg 
      ccaccgaat cgattccaa agacagcat ttgttacgc 
      ctgatgggc attgcgagt ttgtccgca tgccttttg 
      gtctagcca acgctccgg ccgtgtttc agcgagcta 
      tgaataagg tgttgggtc cattacagt ttcagaccg 
      ctttttgtt acatagatg atcttctga taccttcca 
      aagactttg aaacaggtc ttaacaatt tacaaacgg 
      tgtttcaat tgcttcgac agttcggat tgactctaa 
      agctgagta aatattgct tctttggaa gtcaaatag 
      agtatttag ggcatgaga tcagtgcgg aaggcatta 
      agccaggcg agacaaaaa tcaaagcgg tgacagctt 
      ttcccaaac caacagacg tacacaaac ttaggcaat 
      tcttaggtt tgtgtgggt actttcgaa agtacgtga 
      aagattacg caacaatag caaacagtt tgacaagtc 
      tcctaaaga atggcagtg catttgttt tggaagaag 
      tacaagaac gggcttttc agactttaa aagacatct 
      tgacgagca gaccagttc ttgcaattt acgacgcgg 
      aagctgaaa cggagttac acactgacg cttctaaag 
      ttggaattg gtggcattc tgttgcaac gacaaggtg 
      atggatctt tgcgtccag ttatgtttt tcagccgac 
      agacgacca aagaggaac aaaggtacc actcgtacg 
      agctagaaa cgctagcgg tggtctgtt ctctcaaac 
      attatcgag tatacttgt tggggcttc aattcaagg 
      tgatcacag actgtaatg ctttacgaa caaccctta 
      ccaagagag atttgattc cacgaattg ggagatggt 
      ggttgttga cttctgagt tcgacttta ctgtagaat 
      acagacctg gcagtaaaa tgagtcacg ttgacgctt 
      tgagtagaa atacggtat tagatcctt cggagcctt 
      cagtggaag ttttgcata ttaatgtta acgatgatg 
      attggattt tagctgttc aaatgaagg atgcgagat 
      gcgcattgt tgaaggaaa tattggaaa aatcaccga 
      ctaacgctg aagagcgtg ccattcaca aagagtata 
      aactaaaag atggtcgag tattcaaac agactgagt 
      caggattaa aatgggtcg taccaaaag cggtaagac 
      gccagattt tattagcac accacgatg gaattggcc 
      atttatcga atgagaaaa ctttggcat ctgtgtcga 
      aatcttatt ggtttcctt caatgcgga gcaaaaagt 
      ttaagacac attgtaccg atttaaacg ttaaattag 
      tactaaccg cgactgcca caccacggg ctaatggcc 
      aggtggagc gattaaatc gtacgattt tatctcagc 
      ttgctgcta gctcgagag aagaagaga aatgggatg 
      actttgtga gtagaatta gttggggta ttaattcta 
      cgccgaatt cgactacgg gcaaaagtc catacgaat 
      tgttgttgg ggtatacac ctcgacatg ctaatgact 
      caatcctca ccaacgtgg tgacagagg gtgttcatg 
      atggcgatt tgccacgga cacgagcag acgttgctc 
      agcgtgtcg aaaaggaac agcagaagc aaaaggagc 
      gttatgaca agcgacgct gtgccccaa agagattta 
      atgcaggtg atctcgtgt tggtacgca ccacgcgag 
      tttcaaacg agggcaaaa gtcggaagc tgttaccta 
      aatattcgg gaccgtacc gaattcagc aagtgctcg 
      actacgata ggtatgtcg tgacggaca tgccggggg 
      cgacgcgtt cccaaaaac gatatgagg gtgttcact 
      cagtggaca aattgagac attacgttg tcgccacga 
      cgagtggag acgaaacaa taggcgatg agttacgca 
      accacacgc tgtcaattt tcatttggg tgtggctca 
      aaaggcgcg aaaagtttt ctggtgaga ttttcgcaa 
      tggggcgtc acaggtacc gaggaaatg tggactcgg 
      acgactcgg actcaagtg acgatgagc cacggccga 
      aaaggtccg cgcctgggg tctccacta cagtaaatt 
      cacccccag tttcggccg agtccgcta gaatcgcga 
      gacttgagc aacttgtgg aaaacttga atcgacgtt 
      tgctaaacc gcaattttg agcacacat caggacaag 
      ttggcatta aaagtgacc tgattcctg aattcgctc 
      caggaaacc ccaatttta gtaccacta agtggttgc 
      ataaaattg accagttgg ctctcgtga acggttggg 
      acgaacgta caacgattt acggcatga taagtcgac 
      tcaaggggt tggccaagg agtggtacg acaatctcg 
      atccctcag catacgacc ggagctggg atgaatgga 
      aacgtttgt tacaagcca ccttccccg atcaccacg 
      attatgctt ccacacttc tctcctgtc ttattgatg 
      gaatgactg acgtgactc tcagaaatg gggctagag 
      cagggcgtt acgttacac ccgaaagct tgtacgctg 
      agtatttgt cgactctga gatccgaag gtgacaagc 
      gggatacgc tcgcaaagc aggcgcctc gggaaagga 
      ggaggttcc ttccaagtc gagttgaag cacaaaaac 
      tgtattcgt cagttcggt gttacaatt gtcgtaatc 
      gaggacatg ttgcgacaa agtgcccga aaccaagaa 
      ttgagtgca agaaatgtg gtcgcattg gacatgtgg 
      atgctgagt gccgacgtg gtaacgtag taaaagaaa 
      acatgtcta agcaggcac taacgacaa gtgtagctg 
      acgcaagta ataataaga attattaca tagacatta 
      aggtcaatg gtaagccga cgcgagctt acattgact 
      caggagctg aaccggtcc tggtcaagc aaagtgttg 
      ctagagaat tgggactga tgtggcagc caagtttac 
      atttgattc gtggttatg gccagggaa tcaccaaag 
      tgcatgggg aagtcgaag tagagctag aagtggatc 
      ttgtcaagg caaacgtaa aagctttga tcgtcgaag 
      acagtgcac aacgtgtgc ctgtcatag tgggacaga 
      ccttcctga atgcgggtg ctaatacga ttatagcaa 
      atgaggaag ctgtgagag tagttgacg gaaacaacg 
      aatcgatcc aagcatttc taggccaac ttccgacac 
      gaaaagttg cactttggg cggaagagg aaacagtga 
      tccctcctc aatccattg gttgcatac gaatcaagg 
      caaaagacg atgggtgga aaggagctt attacgttg 
      aagggtgtc aacgtccga gaccagggt tcgaacacg 
      ttgttcatc ggtgtgtca catgtgatg gaggcctag 
      tgaccgttc gcaatctat cgcaccaag atctggtct 
      atcgaaagg cgcaaatcg ttgcgagag ctgagcctt 
      gcgagcagg aaaggacag ctacgacgg tgagtgtgt 
      tttctgtgg ggaaggttg aggtaaaga gttttcaac 
      ggtgggaac ttcttacac aagtcaata agaatctag 
      acccggctc agttcgagc aattgttac aactcgtta 
      acgaatttc gagattgtt ttgcacgaa atgtagcgg 
      aaataggtg ccacaactg tggcagaaa tgaagctaa 
      ctttgactg atgataaac ctgtaattt atcgtcctt 
      atcggttat cgcatcacg aaagaagaa ttgttcgag 
      atatagtaa atgacttgt taggtagcg gagtcatac 
      gagaatcag actcgccat attcgagtc ccatactgt 
      tggtgcgga agaaagatg ggcaacacc gtatgtgtg 
      ttgactatc ggcaattaa attcgaaaa caatcaaag 
      atcgctttc cgttaccac gagtagatg aacatttgg 
      ataaactaa acggtgcaa agtttttca ccacactag 
      atctcgcga gtgggtact atcagatcc caatggcca 
      ccgaatcga ttccaaaga cagcatttg ttacgcctg 
      atgggcatt acgagtttg tccgcatgc cttttggtc 
      tagccaacg ctccggcag tgtttcagc gagctatga 
      ataaggtgt tgggtccat tacggtttc agaccgctt 
      tttgttaca tagatgatc ttctgatac cttccaaag 
      actttgaaa caggtctta acaatttac gaacggtgt 
      ttcaattgc ttcgacagt tcggattga ctctaaagc 
      tgagtaaat gttgcttct ttggaagtc aaatagagt 
      atttagggc atgagatca gtgcggaag gcattaagc 
      caggcgaga caaaaatca aagcggtga cagcttttc 
      ccaaaccaa cagacgtac acaaactta ggcaattct 
      taggtttgt gtgggtact ttcgaaagt acgtgaaag 
      attacgcaa caatagcaa acagtttga cgagtctcc 
      taaagaaag gcagtgcat ttgtttggg aagaagcac 
      aagagcggg cttttcaga ctttaaaag acatcttga 
      cgagcagac cagttcttg caatttacg acgcggaag 
      ctgaaacgg agttacaca ctgacgctt ctaaagttg 
      gaattggtg gcattctgt tgcaacgac aaggtgatg 
      gatctttgc gtccagtta tgtttttca gccgacaga 
      cgaccaaag aggaacaaa ggtaccact cgtacgagc 
      tagaaacgc tagcggtgg tctgttctc tcaaacatt 
      atcgagtat acttgttgg ggcttcaat tcaaggtga 
      tcacagact gtaatgctt tacgaacaa cccttacca 
      agagagatt tgattccac gaattggga gatggtggt 
      tgttgactt ctgagttcg actttactg tagaataca 
      gacctggca gtaaaatga gtcacgttg acgctttga 
      gtagaaatc cggtattag atccttcgg agccttcag 
      tggaagtct tgcatatta atgttaacg atgatgatt 
      ggattttag ctgttcaaa tgaaggatg cgagatgcg 
      cattgttga aggaaatat tggaaaaat caccgacga 
      acgctgaag agcgtgcca ttcacaaag agtataaac 
      taaaagatg gtcgagtat tcaaacaga ctgagtcag 
      gattaaaat gggtcgtac caaaagcgg taagacgcc 
      agattttat tagcacacc acgatggaa ttggccatt 
      tatcgaatg agaaaactt tggcatctg tgtcgaaat 
      cttattggt ttccttcaa tgcggagca aaaagttta 
      agacacatt gtaccgatt taaacgtta aattagtac 
      taaccgcga ctgctacac cacgggcta acggccagg 
      tggagcgat tgaatcgta cgattttat ctcagcttg 
      ctgctagct cgagagaag aagagaaat gggatgact 
      ttgtgagta gaattagtt ggggtatta attctacgc 
      cgaattcga ctacgggca aaagtccat acgaattgt 
      tgttggggt atacacctc gacatgcta atgactcaa 
      tcctcacca acgtggtga cagagggtg ttcatgatg 
      gtgatttgc cacggacac gagcagacg ttgctcagc 
      gtgtcgaaa aggaacagc agaagcaaa aagagcgtt 
      atgacaagc gacgctgtg ccccaaaga gatttaatg 
      caggtgatc tcgtgttgg tacgcacca cgcgagctt 
      caaacgagg gcaaaagtc ggaagctgt tacctaaat 
      attcgggac cgtaccgaa ttcagaaag tgctcgact 
      acgataggt accgaggaa atgtggact cggacgact 
      cggactcaa gtgacgatg agccacggc cgaaaaggt 
      ccgcgcctg gggtctcca ctacagtaa attcacccc 
      cagtttcgg ccgagtccg ctagaatcg cgagacttg 
      agcaacttg tggaaaact tgaatcgac gtttgctaa 
      accgtaatt ttgagcaca catcaggac aagttggca 
      ttaaaagtg acttgattc ctgaattcg ctccaggaa 
      accccaatt ttagtacca ctaagtggt tgcataaaa 
      ttgaccagt tggctctcg tgaacggtt gggacgaac 
      gtacaacga tttatggca tgataagtc gactcaagg 
      ggttggcca aggagtggt acgacaatc tcgatccct 
      cagcatatg accggagct gggatgaat ggaaactct 
      cctgtctta ttgatggaa tgactgacg tgactctca 
      gaaatgggg ctagagcag ggcgttacg ttacacccg 
      aaagcttgt acgctgagt atttgtcga ctctgagat 
      ccgaaggtg acaagcggg atacgctcg caaagcagg 
      cgcctcggg aaaggagga ggttccttc caagtcgag 
      tcgaaacac aaaaactgt attctgccc gaaaccaag 
      aattgagtg caagaaatg tggtcgcat tggacatgt 
      ggatgctta gccgacgtg gtaacttag taaaagaaa 
      acatgtcta agcaggcac taacgacaa gtgtagctg 
      acgcaagta ataataaga attattaca tagacatta 
      aggtcaatg gcaagccga cgcgagctt acattgact 
      caggagctg aaccggtcc ttgtcaagc aaagtgttg 
      ctagagaat tgggactga tgtggcagc caagtttac 
      atttgattc gtggttatg gccagggaa tcaccaaag 
      tgcatgggg aagtcgaag tagagctag aagtggatc 
      ttgtcaagg caaacgtaa aagctttga tcgtcgaag 
      acagtgcac aacgtgtgc ctgtcatag tgggacaga 
      ccttcctga atgcgggtg ctaatacga ttatagcaa 
      atgaggaag ctgtgagag tagttgacg gaaacaacg 
      aatcgatcc aagcatttc taggccaac ttccgacac 
      gaaaagttg cactttggg cggaagagg aaacagtga 
      tccctcctc aatccattg gttgcatac gaatcaagg 
      caaaagacg atggcaaaa agtttaaga cacattgta 
      ccgatttaa acgttaaat tagtactaa ccgcgactg 
      ctacaccac gggctaacg gccaggtgg agcgattga 
      atcgtacga ttttatctc agcttgctg ctagctcga 
      gagaagaag agaaatggg atgactttg tgagtagaa 
      ttagttggg gtattaatt ctacgccga attcgacta 
      cgggcaaaa gtccatacg aattgttgt tggggtata 
      cacctcgac atgctaatg actcaatcc tcaccaacg 
      tggtgacag agggtgttc atgatggtg atttgccac 
      ggacacgag cagacgttg ctcagcgtg tcgaaaagg 
      aacagcaga agcaaaaag agcgttatg acaagcgac 
      gctgtgccc caaagagat ttaatgcag gtgatctcg 
      tgttggtac gcaccacgc gagcttcaa acgagggca 
      aaagtcgga agctgttac ctaaatatt cgggaccgt 
      accgaattc agaaagtgc tcgactacg ataggtacg 
      tcgtgacgg acatgccgg gggcgacgc gttcccaaa 
      aacgatatg agggtgttc actcagtgg acaaattga 
      gacattacg ttgtcgcca cgacgagtg gagacgaaa 
      caataggcg atgagttac gcaaccaca cgctgtcaa 
      ttttcattt gggtgtggc tcaaaaggc gcgaaaagt 
      tttctggtg agattttcg caatggggc gtcacaggt 
      accgaggaa atgtggact cggacgact cggactcaa 
      gtgacgatg agccacggc cgaaaaggt ccgcgcctg 
      gggtctcca ctacagtaa attcacccc cagtttcgg 
      ccgagtccg ctagaatcg cgagacttg agcaacttg 
      tggaaaact tgaatcgac gtttgctaa accgcaatt 
      ttgagcaca catcaggac aagttggca ttaaaagtg 
      gcctgattc ctgaattcg ctccaggaa accccaatt 
      ttagtacca ctaagtggt tgcataaaa ttgaccagt 
      tggctctcg tgaacggtt gggacgaac gtacaacga 
      tttacggca tgataagtc gactcaagg ggttggcca 
      aggagtggt acgacaatc tcgatccct cagcatacg 
      accggagct gggatgaat ggaaacgtt tgttacaag 
      ccaccttcc ccgatcacc acgattatg cttccacac 
      ttcgtaagc tagtgaata gggtgaagg aagatgggg 
      aaacctggg cacaatatt attttggga aattaaatc 
      tgttgcagg cttgtgaca taacaggaa cgaatgcag 
      tctcctgtc ttattgatg gaatcactg acgtgactc 
      tcagaaatg gggctagag cagggcgtt acgttacac 
      ccgaaagct tgtacgctg agtatttgt cgactctga 
      gatccgaag gtgacaagc gggatacgc tcgcaaagc 
      aggcgcctc gggaaagga ggaggttcc ttccaagtc 
      gagtcgaag cacaaaaac tgtattcgt cagttcggt 
      gttacaatt gtcgtaatc gaggacatg ttgcgacaa 
      agtgcccga aaccaagaa ttgagtgca agaaatgtg 
      gtcgcattg gacatgtgg atgctgagt gccgacgtg 
      gtaacgtag taaaagaaa acatgtcta agcaggcac 
      taacgacaa gtgtagctg acgcaagta ataataaga 
      attattaca tagacatta aggttaatg gtaagccga 
      cgcgagctt acattgact caggagctg aaccggtcc 
      ttgtcaagc aaagtgttg ctagagaat tgggactga 
      tgtggcagc caagtttac atttgattc gtggttatg 
      gccagggaa tcaccaaag tgcatgggg aagtcgaag 
      tagagctag aagtggatc ttgtcaagg caaacgtaa 
      aagctttga tcgtcgaag acagtgcac aacgtgtgc 
      ctgtcatag tgggacaga ccttcctga atgcgggtg 
      ctaatacga ttatagcaa atgaagaag ctgtgagag 
      tagttgacg gaaacaacg aatcgatcc aagcatttc 
      taggccaac ttccgacac gaaaagttg cactttggg 
      cggaagacg aaacagtga tccctcctc aatccattg 
      gttgcatac gaatcaagg caaaagacg atgggtgga 
      aaggagctt attacgttg aagggtgtc aacgtccga 
      gaccagggt tcgaacacg ttgttcatc ggtgtgtca 
      catgtgatg gaggcctag tgaccgttc gcaatctat 
      cgcaccaag atctggtct atcgaaagg cgcaaatcg 
      ttgcgagag ctgagcctt gcgagcagg aatggacag 
      ctacgacgc ttgctgcta gctcgagag aagaagaga 
      aatgggatg actttgtga gtagaatta gttggggta 
      ttaattcta cgccgaatt cgactacgg gcaaaagtc 
      catacgaat tgttgttgg ggtatacac ctcgacatg 
      ctaatgact caatcctca ccaacgtgg tgacagagg 
      gtgttcatg atggtgatt tgccacgga cacgagcag 
      acgttgctc agcgtgtcg aaaaggaac agcagaagc 
      aaaaagagc gttatgaca agcgacgct gtgccccaa 
      agagattta atgcaggtg atctcgtgt tggtacgca 
      ccacgcgag cttcaaacg agggcaaaa gtcggaagc 
      tgttaccta aatattcgg gaccgtacc gaattcaga 
      aagtgctcg actacgata ggtacgtcg tgacggaca 
      tgccggggg cgacgcgtt cccaaaaac gatatgagg 
      gtgttcact cagtggaca aattgagac attacgttg 
      tcgccacga cgagtggag acgaaacaa taggcgatg 
      tcagtgacg aatgactga gttcgctgt gaagctgag 
      tgaagttca acgtgaact cacgatttt gggcccaaa 
      tgttgtttt taagcgctt tacgttgaa actcat
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