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Gene information
General information
TC007525 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3376211..3400358
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007525
    iBeetle screen iB_04343

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007525 RA
      MKPPPMKIK ARPDGPMMT MDVSKGEAA KPSSGQGPQ 
      ECAGCCKTI TERYLLKAL DLYWHEDCL KCGCCDCRL 
      GEVGSTLYT KANLILCKR DYLRLFGNT GYCAACSKV 
      IPAFEMVMR ARSNVYHLE CFACQQCNH RFCVGDRFY 
      LCENKILCE YDYEERLVF ANMAYNPPP LSHLKRQTS 
      IPPPNIPNH HLINGHRAP LPPTNGDLN NNMSGSGGQ 
      PPPNSMANQ PFQPGHLKN SPMSLGATS
    • >TC007525 RB
      MTMDVSKGE AAKPSSGQG PQECAGCCK TITERYLLK 
      ALDLYWHED CLKCGCCDC RLGEVGSTL YTKANLILC 
      KRDYLRLFG NTGYCAACS KVIPAFEMV MRARSNVYH 
      LECFACQQC NHRFCVGDR FYLCENKIL CEYDYEERL 
      VFANMAYNP PPLSHLKRQ TSIPPPNIP NHHLINGHR 
      APLPPTNGD LNNNMSGSG GQPPPNSMA NQPFQPGHL 
      KNSPMSLGA TS
    CDS sequence
    • >TC007525 RA
      atgaaaccc ccgccaatg aagataaag gccaggcct 
      gacggccct atgatgacc atggacgtg tccaagggg 
      gaggcggcc aagcccagc tcgggccag ggcccgcag 
      gagtgcgcc ggctgctgc aaaaccatc accgagcgg 
      tacctcctg aaggcactg gacctctac tggcacgag 
      gactgcctc aagtgcggc tgctgcgac tgccggctg 
      ggcgaagtc ggctccaca ctctacacc aaggccaat 
      cttatcttg tgcaaaaga gactattta agactgttc 
      ggaaacaca ggctattgt gcggcctgc agcaaagtt 
      ataccggcc ttcgagatg gtcatgagg gccaggagc 
      aacgtctac cacctcgag tgcttcgcc tgccagcag 
      tgcaaccac aggttttgc gtcggcgat cgcttctac 
      ctttgcgaa aacaaaatc ctgtgcgag tacgactac 
      gaagagcgg ctggtgttc gccaacatg gcgtacaac 
      ccgccgcca ctgtcccat ctcaagcgc cagacgtcc 
      attccgccg cccaacatt cccaaccac cacttgata 
      aacgggcac cgggcgccg ctcccgccc accaacggg 
      gacctgaac aacaacatg tcgggctcc ggggggcag 
      ccgcccccg aactccatg gccaaccag ccgttccag 
      cccggacat ctcaaaaac agccccatg tcgctgggg 
      gcgaccagc tga
    • >TC007525 RB
      atgaccatg gacgtgtcc aagggggag gcggccaag 
      cccagctcg ggccagggc ccgcaggag tgcgccggc 
      tgctgcaaa accatcacc gagcggtac ctcctgaag 
      gcactggac ctctactgg cacgaggac tgcctcaag 
      tgcggctgc tgcgactgc cggctgggc gaagtcggc 
      tccacactc tacaccaag gccaatctt atcttgtgc 
      aaaagagac tatttaaga ctgttcgga aacacaggc 
      tattgtgcg gcctgcagc aaagttata ccggccttc 
      gagatggtc atgagggcc aggagcaac gtctaccac 
      ctcgagtgc ttcgcctgc cagcagtgc aaccacagg 
      ttttgcgtc ggcgatcgc ttctacctt tgcgaaaac 
      aaaatcctg tgcgagtac gactacgaa gagcggctg 
      gtgttcgcc aacatggcg tacaacccg ccgccactg 
      tcccatctc aagcgccag acgtccatt ccgccgccc 
      aacattccc aaccaccac ttgataaac gggcaccgg 
      gcgccgctc ccgcccacc aacggggac ctgaacaac 
      aacatgtcg ggctccggg gggcagccg cccccgaac 
      tccatggcc aaccagccg ttccagccc ggacatctc 
      aaaaacagc cccatgtcg ctgggggcg accagctga
    mRNA sequence
    • >TC007525 RA
      aaagctcgc aaagctcgc gacgagaca gcggcggca 
      cgacgcgca ttccgagtc cggacgtcg tcgtgtgaa 
      cacgcacca agactgaaa cgagagcac agaacacga 
      tttcgcgag tgcttgttc ggtttgtgt ttcagtggc 
      gagctttga caacacctg aaaagcaac gccacgaaa 
      gctgtaagt gtcgccctc ggtccggat gaaaccccc 
      gccaatgaa gataaaggc caggcctga cggccctat 
      gatgaccat ggacgtgtc caaggggga ggcggccaa 
      gcccagctc gggccaggg cccgcagga gtgcgccgg 
      ctgctgcaa aaccatcac cgagcggta cctcctgaa 
      ggcactgga cctctactg gcacgagga ctgcctcaa 
      gtgcggctg ctgcgactg ccggctggg cgaagtcgg 
      ctccacact ctacaccaa ggccaatct tatcttgtg 
      caaaagaga ctatttaag actgttcgg aaacacagg 
      ctattgtgc ggcctgcag caaagttat accggcctt 
      cgagatggt catgagggc caggagcaa cgtctacca 
      cctcgagtg cttcgcctg ccagcagtg caaccacag 
      gttttgcgt cggcgatcg cttctacct ttgcgaaaa 
      caaaatcct gtgcgagta cgactacga agagcggct 
      ggtgttcgc caacatggc gtacaaccc gccgccact 
      gtcccatct caagcgcca gacgtccat tccgccgcc 
      caacattcc caaccacca cttgataaa cgggcaccg 
      ggcgccgct cccgcccac caacgggga cctgaacaa 
      caacatgtc gggctccgg ggggcagcc gcccccgaa 
      ctccatggc caaccagcc gttccagcc cggacatct 
      caaaaacag ccccatgtc gctgggggc gaccagctg 
      aagacgatc ccggctgtc gtaaacact gtgattttg 
      tgttttcgt gtgtacata gtctttata aatgctcaa 
      tcactgcgt ataccttcg gatattcga ttaatgtgc 
      aatatttca tcactcaaa ttttgatgt aagataaag 
      acaatccgc tgaatatgt ggtgcagat tttttgggg 
      gttcttgca aagagaggc ggacgaggt cttaagtct 
      tgttagcga actcgacaa ctaattaag tgcaagctt 
      aggcggact aagactttg gaaactcgg aaagtcgcg 
      gttaaatgt gccataaaa taatgtagc tggatcacc 
      ctgtatatg aggccagtt tgcagcaac cgcaaaatt 
      ctatgccag gtattcagt gtccgcctt tgtaattag 
      gtttgaagt ttgtaaata gacactgtt agatttagt 
      aattaatgt tgataacgt ataccatga ttattgacg 
      ctagttgtt ttcaacata aatgttaat tacgatcgc 
      tattattac cgtatatgt tataaaatc gttgccatt 
      tttatttat gttcattgt ttcacactt ttctgttct 
      gagatgtat aaatccctt tgtaaatac tgataacca 
      ctattttta agactaggt gccaatttt agacttttg 
      tgtacaaaa aaagaaacc catccctgt caaaatgta 
      ttcaattat tataccaat gatttgacc aaagaaata 
      aatagattt catgaat
    • >TC007525 RB
      aaagctcgc aaagctcgc gacgagaca gcggcggca 
      cgacgcgca ttccgagtc cggacgtcg tcgtgtgaa 
      cacgcacca agactgaaa cgagagcac agaacacga 
      tttcgcgag tgcttgttc ggtttgtgt ttcagtggc 
      gagctttga caacacctg aaaagcaac gccacgaaa 
      gctataaag gccaggcct gacggccct atgatgacc 
      atggacgtg tccaagggg gaggcggcc aagcccagc 
      tcgggccag ggcccgcag gagtgcgcc ggctgctgc 
      aaaaccatc accgagcgg tacctcctg aaggcactg 
      gacctctac tggcacgag gactgcctc aagtgcggc 
      tgctgcgac tgccggctg ggcgaagtc ggctccaca 
      ctctacacc aaggccaat cttatcttg tgcaaaaga 
      gactattta agactgttc ggaaacaca ggctattgt 
      gcggcctgc agcaaagtt ataccggcc ttcgagatg 
      gtcatgagg gccaggagc aacgtctac cacctcgag 
      tgcttcgcc tgccagcag tgcaaccac aggttttgc 
      gtcggcgat cgcttctac ctttgcgaa aacaaaatc 
      ctgtgcgag tacgactac gaagagcgg ctggtgttc 
      gccaacatg gcgtacaac ccgccgcca ctgtcccat 
      ctcaagcgc cagacgtcc attccgccg cccaacatt 
      cccaaccac cacttgata aacgggcac cgggcgccg 
      ctcccgccc accaacggg gacctgaac aacaacatg 
      tcgggctcc ggggggcag ccgcccccg aactccatg 
      gccaaccag ccgttccag cccggacat ctcaaaaac 
      agccccatg tcgctgggg gcgaccagc tgaagacga 
      tcccggctg tcgtaaaca ctgtgattt tgtgttttc 
      gtgtgtaca tagtcttta taaatgctc aatcactgc 
      gtatacctt cggatattc gattaatgt gcaatattt 
      catcactca aattttgat gtaagataa agacaatcc 
      gctgaatat gtggtgcag attttttgg gggttcttg 
      caaagagag gcggacgag gtcttaagt cttgttagc 
      gaactcgac aactaatta agtgcaagc ttaggcgga 
      ctaagactt tggaaactc ggaaagtcg cggttaaat 
      gtgccataa aataatgta gctggatca ccctgtata 
      tgaggccag tttgcagca accgcaaaa ttctatgcc 
      aggtattca gtgtccgcc tttgtaatt aggtttgaa 
      gtttgtaaa tagacactg ttagattta gtaattaat 
      gttgataac gtataccat gattattga cgctagttg 
      ttttcaaca taaatgtta attacgatc gctattatt 
      accgtatat gttataaaa tcgttgcca tttttattt 
      atgttcatt gtttcacac ttttctgtt ctgagatgt 
      ataaatccc tttgtaaat actgataac cactatttt 
      taagactag gtgccaatt ttagacttt tgtgtacaa 
      aaaaagaaa cccatccct gtcaaaatg tattcaatt 
      attatacca atgatttga ccaaagaaa taaatagat 
      ttcatgaat
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