Status
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Gene information
General information
TC007523 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3407343..3412488
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007523
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04342

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007523 RA
      MGSEKLLFR LEQPHGTGD IYTTWQNGS GMYLATTGI 
      DCMVNIFDR YGQIQDRIR LPSLCTGFG WDSDGDLLA 
      VICQSPQLT LWDANTQKK IQIDVRLKD QMSCVIWAK 
      TAPMLAVGT VKGNISIYN HNTSKRTPV IGKHAKKIT 
      CGAWNTENL LALGSEDKT ISISNVDGD TLRVINLRA 
      EPSEINFSE MKLDERMGG ENTVSVIVG KRTLYLYNL 
      LDPDNPIEL AFQQHYGSI VTYKWFGDG YILIGFNAG 
      YFIAISTHI KEVGQELFQ VRNHKNTLT DITINDKIG 
      KAASCGDNN VKIHDLSNL QETSSVLTL SQEAGLERI 
      SWSVDGQLF SVCTRGGSL NVYLSHVPL LTSVSTPRI 
      AILSSLTEI SLYNYSSEK TKYKPIPIT LETEPSFIA 
      VGPYHIAAG LNNRVWFYD LTRPQPGVD DAPLMLKDR 
      QYLGGVTSI KLNPEYASV LFEGKLQLH MIEQPEVCH 
      EDRETIMFP NANSPNLFI TCHFLTTDF LIYGTDMGN 
      IVYFHVEEW ALAHEYKHE VGIVNVFAD PAGTRLVFV 
      DIKGQGYVY NAVINEVVL IPNLPNKVL GVTWDSNIN 
      DRNIFIVYD QHDIFTYVY VRYSINGSS VQKVGQTSL 
      VTKQIPLLM YSGEVISAT PGGQITQLI LNTHDSPQI 
      GVNEHDQTI QETNFNKQI ALYRFSAAW TTCETLKSE 
      ELWRKLAVE AMKHLEIDL AIRVFKLLD DVSMVWSLE 
      SIAGVEDHK LLCGYVAMF LNDFDLAQD WFMGSSYPV 
      AALEMRRDL LQWDQALQL AKKMAPEQI ALISREYAQ 
      QLEFTGNYS EAHIHYEKG LQEDLSPEH TFICKAGIA 
      RTALHCNNT RHGISIALE LDNKQLLKE CAEILEKNK 
      HLNEAANLF EKCQNYDRA ALNYIKLRN WQKIGELLP 
      KITSNKIHL QYAVAKENE GKYEEAVRA YYTAKDYDS 
      VIRLQLEHL NNPEIAVEL VQETKSTEG AKLVAKFFQ 
      KLNDYTSAI KFLVMSKCN EEAFDLARK HGKMQLYGE 
      ILLNTLAPD ELRPHDFNS IATHFENER NNLLAGIYW 
      FHAKEYAKA MKHLLKAAK SNSKENEAI TAAIDVVAS 
      SKNEALSSQ LIEFLLGES DGLPKDPKY LFRLYMARK 
      QYKEASKSA LIIANEEQI NGNYRNAHD VLFAMYQEL 
      KQNGIKVPN EMQANLMLL HSYILVRLH VKRGDHLKG 
      ARMLIRVAN NISKFPSHK VPILTSTVI ECHRAGLKH 
      AAFKYATML MNPEYRKNV DAKYAKKIE AVVRKPPKS 
      GKAGDGGGD PVEPLTPCP YCENLLPET ETNCNGCKN 
      NIPFCVVTG RHIVTTDLT ACPECDFPA LRSQFIEIL 
      DSEDSCPMC SEKVDSRRL PKIDDPKPY LNVE
    CDS sequence
    • >TC007523 RA
      atgggaagc gaaaagctc ttgttccgc ttggaacag 
      ccccatggc accggtgat atttacacg acatggcaa 
      aatggctct ggcatgtat cttgcaacg actgggatc 
      gactgtatg gtcaatatt ttcgaccgt tatggacaa 
      atccaggac cgtatccgc ttgcccagt ctgtgcacc 
      ggttttggg tgggactcg gacggtgat ctcttggcg 
      gtaatttgc caatcacct caactcaca ctgtgggat 
      gcaaacacc cagaaaaag attcaaatt gatgtgaga 
      ttgaaggac caaatgtcg tgcgtgatt tgggccaag 
      accgccccc atgctggcc gtggggacg gtcaaagga 
      aacatatct atttataat cacaacaca tcaaaacgc 
      actcctgtt attggcaag cacgcgaaa aaaatcacg 
      tgtggggct tggaacacg gagaatttg ctcgcacta 
      gggtctgag gataaaaca atttctatc agtaatgta 
      gacggggat acgctcaga gtgatcaat ttgcgggca 
      gagccgtct gaaataaat ttttcggaa atgaagctc 
      gacgagcgc atggggggc gaaaacact gtcagcgta 
      attgtggga aagcgcact ctctatctt tacaatttg 
      ttagaccct gacaatccc attgaatta gcatttcag 
      cagcattat ggctctatt gttacgtat aagtggttc 
      ggagatggt tatatttta atcggcttc aacgccggt 
      tattttatt gccatctcc acccacatt aaggaagtg 
      ggccaggag ttattccag gtccggaat cacaaaaac 
      accctcact gatattaca ataaacgac aaaatcgga 
      aaagccgca tcttgtggc gataacaat gttaaaata 
      cacgacttg agcaacctc caagaaacc tcaagtgtc 
      ttaactttg tcacaggag gccggttta gagaggata 
      agttggagt gttgacggt cagttgttt tcagtgtgt 
      acacgtggt ggttctttg aacgtctat ttgtcacac 
      gtgccgtta ctaacttca gtctcaacg cctagaata 
      gcgattctg tcaagtctg actgaaatc agtctttac 
      aactattcc tcagaaaaa accaaatac aaaccgatt 
      cctataaca ctcgaaacg gagcctagt tttatcgcc 
      gtgggcccg tatcacata gccgccggt ttaaataac 
      cgggtttgg ttctatgat ttaacgcgc ccccagccg 
      ggagtggat gacgccccc ttaatgctc aaagaccga 
      cagtactta gggggcgtc actagcatc aaactcaat 
      cctgaatat gcttctgtt ttatttgaa ggaaaatta 
      caactacac atgattgaa caaccggag gtttgccat 
      gaggatcgc gaaactata atgtttccg aacgcaaat 
      tcaccaaat ttattcata acttgccat tttttaaca 
      accgatttt ttaatttac ggcaccgat atgggaaat 
      attgtttat ttccacgtc gaagagtgg gctttggcc 
      cacgaatat aagcatgaa gttggcatc gtgaacgtt 
      ttcgccgac cctgccggc actagatta gtttttgtt 
      gatattaaa ggccaaggt tacgtctac aatgctgtg 
      ataaatgaa gttgtactc ataccaaac ctaccaaat 
      aaagtatta ggagtcacg tgggatagc aatataaac 
      gatcgcaac attttcata gtgtacgac caacacgat 
      attttcacc tatgtctac gtccgatac tcaattaat 
      ggttcctcg gtccaaaaa gtgggccaa acctcgctt 
      gtaaccaaa caaatccct ctattaatg tactcgggt 
      gaagtaata tcagccaca cctggcggt cagataact 
      caactaatt ttaaacacc catgactcg cctcaaatc 
      ggcgttaac gaacatgac caaacgatc caagaaacc 
      aattttaac aaacagatc gcattgtat agattttcg 
      gccgcttgg accacttgc gagacgctc aagtcggag 
      gaattgtgg cgaaaactc gcagttgaa gcgatgaaa 
      cacttggaa atagacttg gcaattcgg gtatttaaa 
      ctcctggac gacgtctcc atggtgtgg tctttggag 
      agcatcgcc ggcgtcgaa gaccataaa ttattatgc 
      ggttacgtt gcgatgttt ttgaacgat ttcgatttg 
      gctcaagac tggttcatg ggttcgagt tatccagtg 
      gctgcgcta gaaatgaga cgggatctc ctccagtgg 
      gaccaagcg ttgcaactg gcgaaaaaa atggccccg 
      gaacagatc gcgctgatt tcacgcgaa tacgcccaa 
      cagctcgaa ttcacaggg aactactcc gaagcgcat 
      attcattac gaaaagggg cttcaggag gatttaagc 
      cccgaacat acgtttatt tgcaaggcc ggaatcgcg 
      agaaccgcc ctacattgt aataacacg cgacacggc 
      atcagcata gcgttagaa ttggacaac aaacaactg 
      ttgaaagag tgtgccgag attctcgaa aaaaacaaa 
      cacttaaat gaggctgca aacttgttt gaaaagtgt 
      cagaattac gatcgggcc gctttgaac tacatcaag 
      ctgagaaat tggcagaaa atcggcgag ctgttgccc 
      aaaatcacc tcgaataaa atacatctt cagtacgct 
      gttgcgaaa gaaaacgaa ggtaagtat gaggaggca 
      gtccgggcg tattacaca gccaaggac tacgattcc 
      gtgatacga ctccaactg gaacacctg aacaaccct 
      gaaatcgcc gttgagctg gtccaagag accaagagt 
      acggaaggc gccaaactc gtagcgaaa tttttccaa 
      aaattaaac gactacact tctgccatc aaattcctc 
      gtcatgtcg aaatgtaac gaggaggcg tttgatttg 
      gcccgaaag cacggcaaa atgcaactt tacggcgag 
      attttgctc aataccttg gcaccggat gagttaaga 
      ccgcacgat tttaacagc attgctacg cattttgag 
      aatgaacga aacaattta ctggcgggg atttactgg 
      tttcacgct aaggaatat gccaaggcc atgaaacac 
      ctcctcaag gccgccaaa tcgaattcg aaggaaaac 
      gaagctata actgcagca atagacgtt gtggcgtcc 
      tcaaaaaac gaggcactg tcgtcccaa ctgatcgaa 
      tttcttctg ggcgagtcg gacggcctg ccaaaggac 
      ccgaaatac ctattccgt ctgtacatg gcgcggaag 
      caatacaag gaggcgtca aagtcggcc ctcattatc 
      gcaaacgag gagcaaatc aatggtaat taccggaac 
      gcccacgat gttttattc gcaatgtac caagagctg 
      aagcagaac ggaatcaaa gtccccaac gaaatgcaa 
      gccaatctt atgctactg cacagttac atcctggtg 
      cggctgcat gtcaagagg ggcgaccac ctgaaaggg 
      gcccgaatg ctgatcagg gtggccaac aacatatcg 
      aagtttccg tcccataaa gttcctatt ttaacgtcc 
      acggttata gagtgtcac cgggcgggg ctcaaacat 
      gccgctttt aaatacgcg acgatgttg atgaatccc 
      gagtacagg aaaaatgtc gatgccaaa tacgcgaag 
      aaaattgag gcggtggtg cggaagccg cccaagtcg 
      gggaaggcc ggggacggg ggcggtgac cccgtggag 
      cccctgacg ccgtgtccg tactgcgag aatttgttg 
      cccgagacg gagacgaat tgtaacggt tgtaaaaat 
      aatatcccg ttttgtgtt gttacgggc cgccatatt 
      gttacaacg gacttgacc gcttgtcct gagtgtgat 
      ttcccggct ttgcggtcg cagtttatt gagattctt 
      gatagtgaa gattcctgt cccatgtgt tcggagaag 
      gttgattct cgccggtta cccaaaatt gacgatcct 
      aagccgtat ttgaacgtt gaataa
    mRNA sequence
    • >TC007523 RA
      tctcaaaaa ttgagtttt tgtcaaatt aaaaaacca 
      gattttgaa tcctcatgt tggcgatat gggtgtttg 
      gcgtctgtc gaatgtcaa aatgctttt tcatcaata 
      acaacaatg tttgtatcg tgttttaaa cattaaacg 
      cattgatta ttcaaaaaa tgggaagcg aaaagctct 
      tgttccgct tggaacagc cccatggca ccggtgata 
      tttacacga catggcaaa atggctctg gcatgtatc 
      ttgcaacga ctgggatcg actgtatgg tcaatattt 
      tcgaccgtt atggacaaa tccaggacc gtatccgct 
      tgcccagtc tgtgcaccg gttttgggt gggactcgg 
      acggtgatc tcttggcgg taatttgcc aatcacctc 
      aactcacac tgtgggatg caaacaccc agaaaaaga 
      ttcaaattg atgtgagat tgaaggacc aaatgtcgt 
      gcgtgattt gggccaaga ccgccccca tgctggccg 
      tggggacgg tcaaaggaa acatatcta tttataatc 
      acaacacat caaaacgca ctcctgtta ttggcaagc 
      acgcgaaaa aaatcacgt gtggggctt ggaacacgg 
      agaatttgc tcgcactag ggtctgagg ataaaacaa 
      tttctatca gtaatgtag acggggata cgctcagag 
      tgatcaatt tgcgggcag agccgtctg aaataaatt 
      tttcggaaa tgaagctcg acgagcgca tggggggcg 
      aaaacactg tcagcgtaa ttgtgggaa agcgcactc 
      tctatcttt acaatttgt tagaccctg acaatccca 
      ttgaattag catttcagc agcattatg gctctattg 
      ttacgtata agtggttcg gagatggtt atattttaa 
      tcggcttca acgccggtt attttattg ccatctcca 
      cccacatta aggaagtgg gccaggagt tattccagg 
      tccggaatc acaaaaaca ccctcactg atattacaa 
      taaacgaca aaatcggaa aagccgcat cttgtggcg 
      ataacaatg ttaaaatac acgacttga gcaacctcc 
      aagaaacct caagtgtct taactttgt cacaggagg 
      ccggtttag agaggataa gttggagtg ttgacggtc 
      agttgtttt cagtgtgta cacgtggtg gttctttga 
      acgtctatt tgtcacacg tgccgttac taacttcag 
      tctcaacgc ctagaatag cgattctgt caagtctga 
      ctgaaatca gtctttaca actattcct cagaaaaaa 
      ccaaataca aaccgattc ctataacac tcgaaacgg 
      agcctagtt ttatcgccg tgggcccgt atcacatag 
      ccgccggtt taaataacc gggtttggt tctatgatt 
      taacgcgcc cccagccgg gagtggatg acgccccct 
      taatgctca aagaccgac agtacttag ggggcgtca 
      ctagcatca aactcaatc ctgaatatg cttctgttt 
      tatttgaag gaaaattac aactacaca tgattgaac 
      aaccggagg tttgccatg aggatcgcg aaactataa 
      tgtttccga acgcaaatt caccaaatt tattcataa 
      cttgccatt ttttaacaa ccgattttt taatttacg 
      gcaccgata tgggaaata ttgtttatt tccacgtcg 
      aagagtggg ctttggccc acgaatata agcatgaag 
      ttggcatcg tgaacgttt tcgccgacc ctgccggca 
      ctagattag tttttgttg atattaaag gccaaggtt 
      acgtctaca atgctgtga taaatgaag ttgtactca 
      taccaaacc taccaaata aagtattag gagtcacgt 
      gggatagca atataaacg atcgcaaca ttttcatag 
      tgtacgacc aacacgata ttttcacct atgtctacg 
      tccgatact caattaatg gttcctcgg tccaaaaag 
      tgggccaaa cctcgcttg taaccaaac aaatccctc 
      tattaatgt actcgggtg aagtaatat cagccacac 
      ctggcggtc agataactc aactaattt taaacaccc 
      atgactcgc ctcaaatcg gcgttaacg aacatgacc 
      aaacgatcc aagaaacca attttaaca aacagatcg 
      cattgtata gattttcgg ccgcttgga ccacttgcg 
      agacgctca agtcggagg aattgtggc gaaaactcg 
      cagttgaag cgatgaaac acttggaaa tagacttgg 
      caattcggg tatttaaac tcctggacg acgtctcca 
      tggtgtggt ctttggaga gcatcgccg gcgtcgaag 
      accataaat tattatgcg gttacgttg cgatgtttt 
      tgaacgatt tcgatttgg ctcaagact ggttcatgg 
      gttcgagtt atccagtgg ctgcgctag aaatgagac 
      gggatctcc tccagtggg accaagcgt tgcaactgg 
      cgaaaaaaa tggccccgg aacagatcg cgctgattt 
      cacgcgaat acgcccaac agctcgaat tcacaggga 
      actactccg aagcgcata ttcattacg aaaaggggc 
      ttcaggagg atttaagcc ccgaacata cgtttattt 
      gcaaggccg gaatcgcga gaaccgccc tacattgta 
      ataacacgc gacacggca tcagcatag cgttagaat 
      tggacaaca aacaactgt tgaaagagt gtgccgaga 
      ttctcgaaa aaaacaaac acttaaatg aggctgcaa 
      acttgtttg aaaagtgtc agaattacg atcgggccg 
      ctttgaact acatcaagc tgagaaatt ggcagaaaa 
      tcggcgagc tgttgccca aaatcacct cgaataaaa 
      tacatcttc agtacgctg ttgcgaaag aaaacgaag 
      gtaagtatg aggaggcag tccgggcgt attacacag 
      ccaaggact acgattccg tgatacgac tccaactgg 
      aacacctga acaaccctg aaatcgccg ttgagctgg 
      tccaagaga ccaagagta cggaaggcg ccaaactcg 
      tagcgaaat ttttccaaa aattaaacg actacactt 
      ctgccatca aattcctcg tcatgtcga aatgtaacg 
      aggaggcgt ttgatttgg cccgaaagc acggcaaaa 
      tgcaacttt acggcgaga ttttgctca ataccttgg 
      caccggatg agttaagac cgcacgatt ttaacagca 
      ttgctacgc attttgaga atgaacgaa acaatttac 
      tggcgggga tttactggt ttcacgcta aggaatatg 
      ccaaggcca tgaaacacc tcctcaagg ccgccaaat 
      cgaattcga aggaaaacg aagctataa ctgcagcaa 
      tagacgttg tggcgtcct caaaaaacg aggcactgt 
      cgtcccaac tgatcgaat ttcttctgg gcgagtcgg 
      acggcctgc caaaggacc cgaaatacc tattccgtc 
      tgtacatgg cgcggaagc aatacaagg aggcgtcaa 
      agtcggccc tcattatcg caaacgagg agcaaatca 
      atggtaatt accggaacg cccacgatg ttttattcg 
      caatgtacc aagagctga agcagaacg gaatcaaag 
      tccccaacg aaatgcaag ccaatctta tgctactgc 
      acagttaca tcctggtgc ggctgcatg tcaagaggg 
      gcgaccacc tgaaagggg cccgaatgc tgatcaggg 
      tggccaaca acatatcga agtttccgt cccataaag 
      ttcctattt taacgtcca cggttatag agtgtcacc 
      gggcggggc tcaaacatg ccgctttta aatacgcga 
      cgatgttga tgaatcccg agtacagga aaaatgtcg 
      atgccaaat acgcgaaga aaattgagg cggtggtgc 
      ggaagccgc ccaagtcgg ggaaggccg gggacgggg 
      gcggtgacc ccgtggagc ccctgacgc cgtgtccgt 
      actgcgaga atttgttgc ccgagacgg agacgaatt 
      gtaacggtt gtaaaaata atatcccgt tttgtgttg 
      ttacgggcc gccatattg ttacaacgg acttgaccg 
      cttgtcctg agtgtgatt tcccggctt tgcggtcgc 
      agtttattg agattcttg atagtgaag attcctgtc 
      ccatgtgtt cggagaagg ttgattctc gccggttac 
      ccaaaattg acgatccta agccgtatt tgaacgttg 
      aataacttt tttgatctc gtgattgga gtattattt 
      atttattta ttttagtac aattagatt ttagcaaga 
      agtgatttt agcgaatga aactatccg tccattttt 
      tagtgcaat agactattg ttgaattgt gaaataaaa 
      aatgaaaga aaat
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