| General information | |||
| TC007523 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 3407343..3412488 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007523 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04342 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (16 dpi)
- >TC007523 RA
MGSEKLLFR LEQPHGTGD IYTTWQNGS GMYLATTGI DCMVNIFDR YGQIQDRIR LPSLCTGFG WDSDGDLLA VICQSPQLT LWDANTQKK IQIDVRLKD QMSCVIWAK TAPMLAVGT VKGNISIYN HNTSKRTPV IGKHAKKIT CGAWNTENL LALGSEDKT ISISNVDGD TLRVINLRA EPSEINFSE MKLDERMGG ENTVSVIVG KRTLYLYNL LDPDNPIEL AFQQHYGSI VTYKWFGDG YILIGFNAG YFIAISTHI KEVGQELFQ VRNHKNTLT DITINDKIG KAASCGDNN VKIHDLSNL QETSSVLTL SQEAGLERI SWSVDGQLF SVCTRGGSL NVYLSHVPL LTSVSTPRI AILSSLTEI SLYNYSSEK TKYKPIPIT LETEPSFIA VGPYHIAAG LNNRVWFYD LTRPQPGVD DAPLMLKDR QYLGGVTSI KLNPEYASV LFEGKLQLH MIEQPEVCH EDRETIMFP NANSPNLFI TCHFLTTDF LIYGTDMGN IVYFHVEEW ALAHEYKHE VGIVNVFAD PAGTRLVFV DIKGQGYVY NAVINEVVL IPNLPNKVL GVTWDSNIN DRNIFIVYD QHDIFTYVY VRYSINGSS VQKVGQTSL VTKQIPLLM YSGEVISAT PGGQITQLI LNTHDSPQI GVNEHDQTI QETNFNKQI ALYRFSAAW TTCETLKSE ELWRKLAVE AMKHLEIDL AIRVFKLLD DVSMVWSLE SIAGVEDHK LLCGYVAMF LNDFDLAQD WFMGSSYPV AALEMRRDL LQWDQALQL AKKMAPEQI ALISREYAQ QLEFTGNYS EAHIHYEKG LQEDLSPEH TFICKAGIA RTALHCNNT RHGISIALE LDNKQLLKE CAEILEKNK HLNEAANLF EKCQNYDRA ALNYIKLRN WQKIGELLP KITSNKIHL QYAVAKENE GKYEEAVRA YYTAKDYDS VIRLQLEHL NNPEIAVEL VQETKSTEG AKLVAKFFQ KLNDYTSAI KFLVMSKCN EEAFDLARK HGKMQLYGE ILLNTLAPD ELRPHDFNS IATHFENER NNLLAGIYW FHAKEYAKA MKHLLKAAK SNSKENEAI TAAIDVVAS SKNEALSSQ LIEFLLGES DGLPKDPKY LFRLYMARK QYKEASKSA LIIANEEQI NGNYRNAHD VLFAMYQEL KQNGIKVPN EMQANLMLL HSYILVRLH VKRGDHLKG ARMLIRVAN NISKFPSHK VPILTSTVI ECHRAGLKH AAFKYATML MNPEYRKNV DAKYAKKIE AVVRKPPKS GKAGDGGGD PVEPLTPCP YCENLLPET ETNCNGCKN NIPFCVVTG RHIVTTDLT ACPECDFPA LRSQFIEIL DSEDSCPMC SEKVDSRRL PKIDDPKPY LNVE
- >TC007523 RA
atgggaagc gaaaagctc ttgttccgc ttggaacag ccccatggc accggtgat atttacacg acatggcaa aatggctct ggcatgtat cttgcaacg actgggatc gactgtatg gtcaatatt ttcgaccgt tatggacaa atccaggac cgtatccgc ttgcccagt ctgtgcacc ggttttggg tgggactcg gacggtgat ctcttggcg gtaatttgc caatcacct caactcaca ctgtgggat gcaaacacc cagaaaaag attcaaatt gatgtgaga ttgaaggac caaatgtcg tgcgtgatt tgggccaag accgccccc atgctggcc gtggggacg gtcaaagga aacatatct atttataat cacaacaca tcaaaacgc actcctgtt attggcaag cacgcgaaa aaaatcacg tgtggggct tggaacacg gagaatttg ctcgcacta gggtctgag gataaaaca atttctatc agtaatgta gacggggat acgctcaga gtgatcaat ttgcgggca gagccgtct gaaataaat ttttcggaa atgaagctc gacgagcgc atggggggc gaaaacact gtcagcgta attgtggga aagcgcact ctctatctt tacaatttg ttagaccct gacaatccc attgaatta gcatttcag cagcattat ggctctatt gttacgtat aagtggttc ggagatggt tatatttta atcggcttc aacgccggt tattttatt gccatctcc acccacatt aaggaagtg ggccaggag ttattccag gtccggaat cacaaaaac accctcact gatattaca ataaacgac aaaatcgga aaagccgca tcttgtggc gataacaat gttaaaata cacgacttg agcaacctc caagaaacc tcaagtgtc ttaactttg tcacaggag gccggttta gagaggata agttggagt gttgacggt cagttgttt tcagtgtgt acacgtggt ggttctttg aacgtctat ttgtcacac gtgccgtta ctaacttca gtctcaacg cctagaata gcgattctg tcaagtctg actgaaatc agtctttac aactattcc tcagaaaaa accaaatac aaaccgatt cctataaca ctcgaaacg gagcctagt tttatcgcc gtgggcccg tatcacata gccgccggt ttaaataac cgggtttgg ttctatgat ttaacgcgc ccccagccg ggagtggat gacgccccc ttaatgctc aaagaccga cagtactta gggggcgtc actagcatc aaactcaat cctgaatat gcttctgtt ttatttgaa ggaaaatta caactacac atgattgaa caaccggag gtttgccat gaggatcgc gaaactata atgtttccg aacgcaaat tcaccaaat ttattcata acttgccat tttttaaca accgatttt ttaatttac ggcaccgat atgggaaat attgtttat ttccacgtc gaagagtgg gctttggcc cacgaatat aagcatgaa gttggcatc gtgaacgtt ttcgccgac cctgccggc actagatta gtttttgtt gatattaaa ggccaaggt tacgtctac aatgctgtg ataaatgaa gttgtactc ataccaaac ctaccaaat aaagtatta ggagtcacg tgggatagc aatataaac gatcgcaac attttcata gtgtacgac caacacgat attttcacc tatgtctac gtccgatac tcaattaat ggttcctcg gtccaaaaa gtgggccaa acctcgctt gtaaccaaa caaatccct ctattaatg tactcgggt gaagtaata tcagccaca cctggcggt cagataact caactaatt ttaaacacc catgactcg cctcaaatc ggcgttaac gaacatgac caaacgatc caagaaacc aattttaac aaacagatc gcattgtat agattttcg gccgcttgg accacttgc gagacgctc aagtcggag gaattgtgg cgaaaactc gcagttgaa gcgatgaaa cacttggaa atagacttg gcaattcgg gtatttaaa ctcctggac gacgtctcc atggtgtgg tctttggag agcatcgcc ggcgtcgaa gaccataaa ttattatgc ggttacgtt gcgatgttt ttgaacgat ttcgatttg gctcaagac tggttcatg ggttcgagt tatccagtg gctgcgcta gaaatgaga cgggatctc ctccagtgg gaccaagcg ttgcaactg gcgaaaaaa atggccccg gaacagatc gcgctgatt tcacgcgaa tacgcccaa cagctcgaa ttcacaggg aactactcc gaagcgcat attcattac gaaaagggg cttcaggag gatttaagc cccgaacat acgtttatt tgcaaggcc ggaatcgcg agaaccgcc ctacattgt aataacacg cgacacggc atcagcata gcgttagaa ttggacaac aaacaactg ttgaaagag tgtgccgag attctcgaa aaaaacaaa cacttaaat gaggctgca aacttgttt gaaaagtgt cagaattac gatcgggcc gctttgaac tacatcaag ctgagaaat tggcagaaa atcggcgag ctgttgccc aaaatcacc tcgaataaa atacatctt cagtacgct gttgcgaaa gaaaacgaa ggtaagtat gaggaggca gtccgggcg tattacaca gccaaggac tacgattcc gtgatacga ctccaactg gaacacctg aacaaccct gaaatcgcc gttgagctg gtccaagag accaagagt acggaaggc gccaaactc gtagcgaaa tttttccaa aaattaaac gactacact tctgccatc aaattcctc gtcatgtcg aaatgtaac gaggaggcg tttgatttg gcccgaaag cacggcaaa atgcaactt tacggcgag attttgctc aataccttg gcaccggat gagttaaga ccgcacgat tttaacagc attgctacg cattttgag aatgaacga aacaattta ctggcgggg atttactgg tttcacgct aaggaatat gccaaggcc atgaaacac ctcctcaag gccgccaaa tcgaattcg aaggaaaac gaagctata actgcagca atagacgtt gtggcgtcc tcaaaaaac gaggcactg tcgtcccaa ctgatcgaa tttcttctg ggcgagtcg gacggcctg ccaaaggac ccgaaatac ctattccgt ctgtacatg gcgcggaag caatacaag gaggcgtca aagtcggcc ctcattatc gcaaacgag gagcaaatc aatggtaat taccggaac gcccacgat gttttattc gcaatgtac caagagctg aagcagaac ggaatcaaa gtccccaac gaaatgcaa gccaatctt atgctactg cacagttac atcctggtg cggctgcat gtcaagagg ggcgaccac ctgaaaggg gcccgaatg ctgatcagg gtggccaac aacatatcg aagtttccg tcccataaa gttcctatt ttaacgtcc acggttata gagtgtcac cgggcgggg ctcaaacat gccgctttt aaatacgcg acgatgttg atgaatccc gagtacagg aaaaatgtc gatgccaaa tacgcgaag aaaattgag gcggtggtg cggaagccg cccaagtcg gggaaggcc ggggacggg ggcggtgac cccgtggag cccctgacg ccgtgtccg tactgcgag aatttgttg cccgagacg gagacgaat tgtaacggt tgtaaaaat aatatcccg ttttgtgtt gttacgggc cgccatatt gttacaacg gacttgacc gcttgtcct gagtgtgat ttcccggct ttgcggtcg cagtttatt gagattctt gatagtgaa gattcctgt cccatgtgt tcggagaag gttgattct cgccggtta cccaaaatt gacgatcct aagccgtat ttgaacgtt gaataa
- >TC007523 RA
tctcaaaaa ttgagtttt tgtcaaatt aaaaaacca gattttgaa tcctcatgt tggcgatat gggtgtttg gcgtctgtc gaatgtcaa aatgctttt tcatcaata acaacaatg tttgtatcg tgttttaaa cattaaacg cattgatta ttcaaaaaa tgggaagcg aaaagctct tgttccgct tggaacagc cccatggca ccggtgata tttacacga catggcaaa atggctctg gcatgtatc ttgcaacga ctgggatcg actgtatgg tcaatattt tcgaccgtt atggacaaa tccaggacc gtatccgct tgcccagtc tgtgcaccg gttttgggt gggactcgg acggtgatc tcttggcgg taatttgcc aatcacctc aactcacac tgtgggatg caaacaccc agaaaaaga ttcaaattg atgtgagat tgaaggacc aaatgtcgt gcgtgattt gggccaaga ccgccccca tgctggccg tggggacgg tcaaaggaa acatatcta tttataatc acaacacat caaaacgca ctcctgtta ttggcaagc acgcgaaaa aaatcacgt gtggggctt ggaacacgg agaatttgc tcgcactag ggtctgagg ataaaacaa tttctatca gtaatgtag acggggata cgctcagag tgatcaatt tgcgggcag agccgtctg aaataaatt tttcggaaa tgaagctcg acgagcgca tggggggcg aaaacactg tcagcgtaa ttgtgggaa agcgcactc tctatcttt acaatttgt tagaccctg acaatccca ttgaattag catttcagc agcattatg gctctattg ttacgtata agtggttcg gagatggtt atattttaa tcggcttca acgccggtt attttattg ccatctcca cccacatta aggaagtgg gccaggagt tattccagg tccggaatc acaaaaaca ccctcactg atattacaa taaacgaca aaatcggaa aagccgcat cttgtggcg ataacaatg ttaaaatac acgacttga gcaacctcc aagaaacct caagtgtct taactttgt cacaggagg ccggtttag agaggataa gttggagtg ttgacggtc agttgtttt cagtgtgta cacgtggtg gttctttga acgtctatt tgtcacacg tgccgttac taacttcag tctcaacgc ctagaatag cgattctgt caagtctga ctgaaatca gtctttaca actattcct cagaaaaaa ccaaataca aaccgattc ctataacac tcgaaacgg agcctagtt ttatcgccg tgggcccgt atcacatag ccgccggtt taaataacc gggtttggt tctatgatt taacgcgcc cccagccgg gagtggatg acgccccct taatgctca aagaccgac agtacttag ggggcgtca ctagcatca aactcaatc ctgaatatg cttctgttt tatttgaag gaaaattac aactacaca tgattgaac aaccggagg tttgccatg aggatcgcg aaactataa tgtttccga acgcaaatt caccaaatt tattcataa cttgccatt ttttaacaa ccgattttt taatttacg gcaccgata tgggaaata ttgtttatt tccacgtcg aagagtggg ctttggccc acgaatata agcatgaag ttggcatcg tgaacgttt tcgccgacc ctgccggca ctagattag tttttgttg atattaaag gccaaggtt acgtctaca atgctgtga taaatgaag ttgtactca taccaaacc taccaaata aagtattag gagtcacgt gggatagca atataaacg atcgcaaca ttttcatag tgtacgacc aacacgata ttttcacct atgtctacg tccgatact caattaatg gttcctcgg tccaaaaag tgggccaaa cctcgcttg taaccaaac aaatccctc tattaatgt actcgggtg aagtaatat cagccacac ctggcggtc agataactc aactaattt taaacaccc atgactcgc ctcaaatcg gcgttaacg aacatgacc aaacgatcc aagaaacca attttaaca aacagatcg cattgtata gattttcgg ccgcttgga ccacttgcg agacgctca agtcggagg aattgtggc gaaaactcg cagttgaag cgatgaaac acttggaaa tagacttgg caattcggg tatttaaac tcctggacg acgtctcca tggtgtggt ctttggaga gcatcgccg gcgtcgaag accataaat tattatgcg gttacgttg cgatgtttt tgaacgatt tcgatttgg ctcaagact ggttcatgg gttcgagtt atccagtgg ctgcgctag aaatgagac gggatctcc tccagtggg accaagcgt tgcaactgg cgaaaaaaa tggccccgg aacagatcg cgctgattt cacgcgaat acgcccaac agctcgaat tcacaggga actactccg aagcgcata ttcattacg aaaaggggc ttcaggagg atttaagcc ccgaacata cgtttattt gcaaggccg gaatcgcga gaaccgccc tacattgta ataacacgc gacacggca tcagcatag cgttagaat tggacaaca aacaactgt tgaaagagt gtgccgaga ttctcgaaa aaaacaaac acttaaatg aggctgcaa acttgtttg aaaagtgtc agaattacg atcgggccg ctttgaact acatcaagc tgagaaatt ggcagaaaa tcggcgagc tgttgccca aaatcacct cgaataaaa tacatcttc agtacgctg ttgcgaaag aaaacgaag gtaagtatg aggaggcag tccgggcgt attacacag ccaaggact acgattccg tgatacgac tccaactgg aacacctga acaaccctg aaatcgccg ttgagctgg tccaagaga ccaagagta cggaaggcg ccaaactcg tagcgaaat ttttccaaa aattaaacg actacactt ctgccatca aattcctcg tcatgtcga aatgtaacg aggaggcgt ttgatttgg cccgaaagc acggcaaaa tgcaacttt acggcgaga ttttgctca ataccttgg caccggatg agttaagac cgcacgatt ttaacagca ttgctacgc attttgaga atgaacgaa acaatttac tggcgggga tttactggt ttcacgcta aggaatatg ccaaggcca tgaaacacc tcctcaagg ccgccaaat cgaattcga aggaaaacg aagctataa ctgcagcaa tagacgttg tggcgtcct caaaaaacg aggcactgt cgtcccaac tgatcgaat ttcttctgg gcgagtcgg acggcctgc caaaggacc cgaaatacc tattccgtc tgtacatgg cgcggaagc aatacaagg aggcgtcaa agtcggccc tcattatcg caaacgagg agcaaatca atggtaatt accggaacg cccacgatg ttttattcg caatgtacc aagagctga agcagaacg gaatcaaag tccccaacg aaatgcaag ccaatctta tgctactgc acagttaca tcctggtgc ggctgcatg tcaagaggg gcgaccacc tgaaagggg cccgaatgc tgatcaggg tggccaaca acatatcga agtttccgt cccataaag ttcctattt taacgtcca cggttatag agtgtcacc gggcggggc tcaaacatg ccgctttta aatacgcga cgatgttga tgaatcccg agtacagga aaaatgtcg atgccaaat acgcgaaga aaattgagg cggtggtgc ggaagccgc ccaagtcgg ggaaggccg gggacgggg gcggtgacc ccgtggagc ccctgacgc cgtgtccgt actgcgaga atttgttgc ccgagacgg agacgaatt gtaacggtt gtaaaaata atatcccgt tttgtgttg ttacgggcc gccatattg ttacaacgg acttgaccg cttgtcctg agtgtgatt tcccggctt tgcggtcgc agtttattg agattcttg atagtgaag attcctgtc ccatgtgtt cggagaagg ttgattctc gccggttac ccaaaattg acgatccta agccgtatt tgaacgttg aataacttt tttgatctc gtgattgga gtattattt atttattta ttttagtac aattagatt ttagcaaga agtgatttt agcgaatga aactatccg tccattttt tagtgcaat agactattg ttgaattgt gaaataaaa aatgaaaga aaat
