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Gene information
General information
TC007514 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3471766..3473118
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007514
    iBeetle screen iB_04341

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007514 RA
      MDLHKPVNI ANKSVEELT PEEKWRLEH QKLHEQHKG 
      HEKMHAEMV LILILTLVV AQVVLIEWK KRHSRSYLL 
      VTLLGMWII PLILCLHNR WWRFIFIWL LFSCVTSLI 
      VWKAMQKPI TGTTPRLVY KWFLFIYKL SYVLGILGY 
      FIMMATFFG LNLVFNAKA NSWMDCALL LIFYGLYYG 
      VLGRDISEI CADKMASHV GYYTNQGMP TRNLDPSVC 
      AVCGNKLLV SENEEGVIE NTYKLTCDH VFHEFCIRG 
      WCIVGKKQT CPYCKEKVD LKKMFCNPW EKPHVLYGQ 
      LLDWIRWLV AWQPMILFL VQGINWVLG LE
    CDS sequence
    • >TC007514 RA
      atggatttg cacaaaccg gtgaatatt gcgaataag 
      tcggtcgag gaattaaca cctgaggaa aaatggagg 
      ctagagcat cagaaatta cacgagcaa cacaaaggc 
      cacgaaaaa atgcacgcg gagatggtc cttatccta 
      attctaaca ctagtagta gcgcaagta gttttaatt 
      gaatggaaa aaacggcat tcccgctcg tatctttta 
      gtaacgctc ctcggaatg tggataatt cccttaatt 
      ctctgcctg cacaatagg tggtggcgt ttcatcttc 
      atttggctg ctcttttcc tgtgtcacg agtctaatc 
      gtgtggaaa gcgatgcaa aaacccatc acggggacc 
      accccacga ctagtctat aagtggttt ctttttatc 
      tataagcta agctacgtt ctgggtatc ttgggctac 
      tttatcatg atggcgact tttttcggt ctcaatcta 
      gtttttaac gccaaagcc aactcttgg atggattgc 
      gctttgctg ttaattttc tacggcttg tactacggc 
      gttttgggg cgcgacatt tccgaaata tgcgccgat 
      aaaatggct tcgcatgtt gggtattac actaatcaa 
      ggcatgccg acgcgaaat ttagacccc tcggtgtgt 
      gccgtttgc ggaaataaa ttactagtc agcgagaat 
      gaagagggg gtcattgaa aacacctac aaactgacc 
      tgtgaccac gtgtttcat gaattttgc atacggggg 
      tggtgcatc gtaggcaag aaacagacg tgtccttac 
      tgcaaggaa aaagttgac ttgaaaaaa atgttctgc 
      aacccatgg gagaaacca cacgtgctt tatgggcag 
      ctgctggac tggattagg tggctggtg gcgtggcaa 
      cccatgatc cttttcctg gtgcagggg attaactgg 
      gtcctgggg ctagaataa
    mRNA sequence
    • >TC007514 RA
      tgtcacttg tcaaaaaaa tgacataac ctgaaagcc 
      gcgtcattg tattataaa taataaatg tttttaata 
      tttttctat ggaattaaa taaatttgc tagtcaaat 
      aagttaagg tttaagtat actacgcaa tggatttgc 
      acaaaccgg tgaatattg cgaataagt cggtcgagg 
      aattaacac ctgaggaaa aatggaggc tagagcatc 
      agaaattac acgagcaac acaaaggcc acgaaaaaa 
      tgcacgcgg agatggtcc ttatcctaa ttctaacac 
      tagtagtag cgcaagtag ttttaattg aatggaaaa 
      aacggcatt cccgctcgt atcttttag taacgctcc 
      tcggaatgt ggataattc ccttaattc tctgcctgc 
      acaataggt ggtggcgtt tcatcttca tttggctgc 
      tcttttcct gtgtcacga gtctaatcg tgtggaaag 
      cgatgcaaa aacccatca cggggacca ccccacgac 
      tagtctata agtggtttc tttttatct ataagctaa 
      gctacgttc tgggtatct tgggctact ttatcatga 
      tggcgactt ttttcggtc tcaatctag tttttaacg 
      ccaaagcca actcttgga tggattgcg ctttgctgt 
      taattttct acggcttgt actacggcg ttttggggc 
      gcgacattt ccgaaatat gcgccgata aaatggctt 
      cgcatgttg ggtattaca ctaatcaag gcatgccga 
      cgcgaaatt tagacccct cggtgtgtg ccgtttgcg 
      gaaataaat tactagtca gcgagaatg aagaggggg 
      tcattgaaa acacctaca aactgacct gtgaccacg 
      tgtttcatg aattttgca tacgggggt ggtgcatcg 
      taggcaaga aacagacgt gtccttact gcaaggaaa 
      aagttgact tgaaaaaaa tgttctgca acccatggg 
      agaaaccac acgtgcttt atgggcagc tgctggact 
      ggattaggt ggctggtgg cgtggcaac ccatgatcc 
      ttttcctgg tgcagggga ttaactggg tcctggggc 
      tagaataag ggtttaagc aattaaaca ttgttttaa 
      aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY