Status
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Gene information
General information
TC032794
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007496,TC007495
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3732974..3748653
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032794
    iBeetle screen iB_04339

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032794 RA
      MSKVTHTSL VRKKTHLHN WPGFGVLKT YQDADYKVG 
      GFARIGWVQ RIACRVRPR VGLISAKVP NTGTGLSNL 
      ALPGCGLAI PPLRSGSVR LLWQGLYRS AVGTRAVNH 
      KHAIAASWK SAKPRQIVA FRAEEPFRT LRVFHRHDR 
      GVFAISKVL KVCSKSRNM STAIMSRYD FGDNLMKSA 
      VSSRDAAPG SPLDLGSAG MRFALTRMA STPATNQNL 
      TLLLGNLTQ HRRLERAQS APAPSAAAA AAASVAASA 
      VNTSRYKTE LCRPYEEFG VCKYGDKCQ FAHGGAELR 
      SLARHPKYK TELCRTYHT VGFCPYGPR CHFVHNQDE 
      VVLQKQALA RARPRPAAL SPTLSLDSS SSPPCSLSQ 
      SPTSSMGSF FSSEPELHS PAAAAPTDD RLPVFNRLA 
      EVNRMHAMK IGDLVL
    CDS sequence
    • >TC032794 RA
      atgtcaaaa gttacacat acatcccta gtgcgaaaa 
      aaaacgcat ttgcataac tggccaggt ttcggtgtt 
      ttgaagacg taccaggat gcggactac aaggtcggg 
      ggttttgcg cgaatagga tgggtccaa agaatcgcg 
      tgccgcgtt cggcctcga gtcggcctt atctccgcc 
      aaagttccg aacactggc acgggactt tcgaactta 
      gcactgccc ggctgtgga ttggccatc cctcctctg 
      cgtagtggg agcgttcga ctactttgg cagggattg 
      tatcgctca gcagtcggc acaagagct gtgaatcat 
      aaacacgcg atcgctgcg tcgtggaaa agcgcgaaa 
      ccacgtcaa atagttgca tttcgagcc gaagaacct 
      ttccgaacg ttgcgagtc ttccatcgg cacgatcgc 
      ggtgttttt gccatttca aaagtgttg aaagtgtgc 
      tcaaaatca aggaatatg tctactgcg attatgtca 
      cgatatgat ttcggggat aatctgatg aagagtgcg 
      gtgtcatcc cgagacgcc gcccccggc agccccctg 
      gacctggga tccgccggg atgcggttc gcactgacg 
      cggatggcg tcgacgccg gcgaccaac cagaacctg 
      accctcctc ctcggaaac ttgacgcag caccggcgc 
      ctcgaacgc gcgcaatcg gcgccggcc cccagcgcc 
      gccgccgcg gccgccgcc agcgtcgcc gcctccgcc 
      gtcaacacc tcccggtac aaaacggag ctgtgtcgg 
      ccgtacgaa gagtttggg gtgtgcaaa tacggcgac 
      aagtgtcag ttcgcgcac ggcggcgcc gagttgcgg 
      tcgctggcg cgccacccc aagtacaag acggagctg 
      tgccgcacg taccacacg gtgggcttc tgtccgtac 
      ggcccccgg tgccatttc gtgcacaac caggacgag 
      gtggtgctg cagaagcag gcgctggcg agggcgcgg 
      ccccggccg gcggcgctc agccccacg ctgtcgctg 
      gacagcagc agcagtccc ccgtgcagt ctgagtcag 
      tcgccgacg tcgtcgatg ggctcgttc ttcagcagc 
      gagcccgag ctgcactcg cctgctgct gccgccccc 
      accgacgac cgcctcccg gtgttcaac aggttggcc 
      gaggtgaat cgaatgcac gccatgaaa atcggggac 
      ctggtgctc tag
    mRNA sequence
    • >TC032794 RA
      cacgaattt tataaataa aaatccaat gtttttcta 
      tcattttcg caacgcagg tatttccag tttctacgt 
      cactattaa atcaaatgt caaaagtta cacatacat 
      ccctagtgc gaaaaaaaa cgcatttgc ataactggc 
      caggtttcg gtgttttga agacgtacc aggatgcgg 
      actacaagg tcgggggtt ttgcgcgaa taggatggg 
      tccaaagaa tcgcgtgcc gcgttcggc ctcgagtcg 
      gccttatct ccgccaaag ttccgaaca ctggcacgg 
      gactttcga acttagcac tgcccggct gtggattgg 
      ccatccctc ctctgcgta gtgggagcg ttcgactac 
      tttggcagg gattgtatc gctcagcag tcggcacaa 
      gagctgtga atcataaac acgcgatcg ctgcgtcgt 
      ggaaaagcg cgaaaccac gtcaaatag ttgcatttc 
      gagccgaag aacctttcc gaacgttgc gagtcttcc 
      atcggcacg atcgcggtg tttttgcca tttcaaaag 
      tgttgaaag tgtgctcaa aatcaagga atatgtcta 
      ctgcgatta tgtcacgat atgatttcg gggataatc 
      tgatgaaga gtgcggtgt catcccgag acgccgccc 
      ccggcagcc ccctggacc tgggatccg ccgggatgc 
      ggttcgcac tgacgcgga tggcgtcga cgccggcga 
      ccaaccaga acctgaccc tcctcctcg gaaacttga 
      cgcagcacc ggcgcctcg aacgcgcgc aatcggcgc 
      cggccccca gcgccgccg ccgcggccg ccgccagcg 
      tcgccgcct ccgccgtca acacctccc ggtacaaaa 
      cggagctgt gtcggccgt acgaagagt ttggggtgt 
      gcaaatacg gcgacaagt gtcagttcg cgcacggcg 
      gcgccgagt tgcggtcgc tggcgcgcc accccaagt 
      acaagacgg agctgtgcc gcacgtacc acacggtgg 
      gcttctgtc cgtacggcc cccggtgcc atttcgtgc 
      acaaccagg acgaggtgg tgctgcaga agcaggcgc 
      tggcgaggg cgcggcccc ggccggcgg cgctcagcc 
      ccacgctgt cgctggaca gcagcagca gtcccccgt 
      gcagtctga gtcagtcgc cgacgtcgt cgatgggct 
      cgttcttca gcagcgagc ccgagctgc actcgcctg 
      ctgctgccg cccccaccg acgaccgcc tcccggtgt 
      tcaacaggt tggccgagg tgaatcgaa tgcacgcca 
      tgaaaatcg gggacctgg tgctctagt tactatatt 
      attaagtgc aacaaccac tcaaagact taactattt 
      atgatgtta cagtaggtc cgcccgtcg gcggcgtga 
      ttttgatat tgtgtgatt tctaaatat ttaaaaaga 
      tgataatat tattatatt agttagagg cgtgtttta 
      tacgaatgt tgattgtgt acgtgcaaa aaacctagg 
      cttagtctt ttccttcag tcgtcggtc tcgatttct 
      gtaacattc cgtgatcga agtttattt attatttta 
      ttacttgct tttctcgtt tgtgtgact tgtgaccga 
      tgaaagaat acgtttttt tgttattta aatcattat 
      ttaattttt atgtttctc attcgacac gttatgtta 
      tttagttat tttattatt ataatttat atgtaactt 
      atttatacc ttgtcgcat atcgcaata ctgatttta 
      ttgatgatg tgtagtgga cgactgtgc tggcttttg 
      gcacacaag ttggatgcc ccaacaaag gccacctct 
      gtatattat tatttatat ttgtatttc ttgtatcat 
      tatcaataa attatattt tgttct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY