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Gene information
General information
TC007477 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3905708..3914473
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007477
    iBeetle screen iB_04337

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007477 RA
      MGLVRAAAA ITPVLPSEE VNYSDAGSE TDESDWSGI 
      LTYQILGVI FLTAMALVL LAAVSYQCA ATWKWIRDK 
      TCGDPSDAE TSINKQQAA IRISHSLPD LQSEPMTQE 
      YVQEHKDSM AKKVLRQTT LPIVPTRHQ TFQRQLSHR 
      LEMPSNVQF SICTLEHRS DSSIVGLIK PELYKKDLV 
      RQSSADSSG GPEMEYCGK LHFSLRYDK DVEGLVVRV 
      FEGRDLPIK DSTGSSDPY IKLYLLPDR KKKFQTKVH 
      RKNLNPVFN ETFIFSVPY EELRQRYLQ FSVYDFDRF 
      SRHDLIGHV VLKGLLDAT DLQQEIEYT MNILCPPQE 
      KVDLGELML SLCYLPTAG RLTITIIKG RSLKAMDIT 
      GKSDPYVKV YLLCQGKRI KKKKTSVKK NTLNPVYNE 
      ALVFDVPAE NIEDVSLIV KVIDYDRIG SNELMGCTA 
      IGSSFIGIG KDHWLEMLD NPRCPVAQW YPLLESIPG 
      HIPANDSTP LSLSCLNR
    CDS sequence
    • >TC007477 RA
      atgggactt gtgagggct gctgctgcc atcacgccc 
      gtccttcct agcgaagaa gtcaactat tcggacgcc 
      ggtagtgaa acagacgaa tcagactgg tcaggtatc 
      ctaacatat caaatattg ggtgtgata tttctgacg 
      gcgatggcg ttagttctt ctagcggct gtgagttat 
      caatgcgcc gccacgtgg aaatggata agagacaaa 
      acctgcggc gacccttcg gacgccgag acctccatc 
      aacaagcaa caagccgct atcagaatc agtcactct 
      ctaccggac ttgcaatcg gagcccatg acccaggag 
      tacgtccaa gagcacaaa gactcgatg gccaagaag 
      gtcctgcgc caaaccacc ttgcccatc gtgcccacc 
      aggcaccag accttccaa cggcaactg tcccatcgg 
      ctggagatg ccctcgaac gtccagttc agcatctgc 
      acgctggag cacaggagc gactcgagt atcgttggg 
      ctgattaag cccgagttg tataagaaa gatttggtc 
      cgccagtcg tccgctgac agctccggg ggtcccgag 
      atggaatac tgtggcaaa ttgcatttc tcgctgcga 
      tacgacaaa gacgtagaa gggcttgtc gtgagggtg 
      ttcgagggc cgcgatttg cccatcaag gactccacg 
      ggcagcagc gacccctac ataaaactc tacctcctg 
      ccggaccgg aaaaagaaa ttccagacg aaagtccac 
      cggaaaaac ctcaatccg gtgtttaac gagacgttt 
      atattcagt gttccctac gaggagctg cggcagcgg 
      tacctccag ttctccgtg tacgacttc gaccgcttc 
      tcgcggcac gacctcatc gggcacgtc gtcctcaag 
      ggcctcctg gacgccacg gacctgcag caggagatc 
      gagtacacg atgaacatc ctctgcccg ccacaggaa 
      aaagtcgat ttgggcgag ctgatgctg tcgctttgt 
      tatctgccg acggccgga agactgacc atcacgata 
      attaagggg aggagtctc aaagcgatg gatattacg 
      ggaaaatca gatccttac gttaaagtg tatttgctt 
      tgtcaaggc aagcggata aagaagaaa aagacgtcg 
      gtgaagaaa aatacgctc aacccggtg tataacgag 
      gcgttggtc tttgatgtc ccggctgag aatatcgag 
      gatgtttcc ctcattgtt aaagtgata gactacgac 
      agaatcggt tcaaatgag ctgatgggc tgcacggcg 
      ataggttca agtttcata ggaatcggc aaggaccac 
      tggttagaa atgctggac aatccccgg tgccccgtg 
      gcccaatgg taccccctc ctcgaatcg ataccgggc 
      cacataccc gccaacgac tccaccccc ctcagcctc 
      agctgcctc aacaggtga
    mRNA sequence
    • >TC007477 RA
      gaaataaat ccaagagcg aatttccaa cttttactg 
      ttccttgct atcggatgc accaatctc agctctcat 
      tccttctcc aatttccat cccaagaac ggccggttg 
      tctgatttg gttgcattt ccttcctgc tttatcgga 
      atcctttgg gaagttggg gattttgtt ccggtgaaa 
      tcgtggcgc ttccctggt ctacctttc gccgactgg 
      ccttaaccc agcctcggc gacattgaa cctggccaa 
      tccgtggct aggtcgtcg gtgacgtct cgctgcgtg 
      cgtcgctac gcgccaact ttcaggcga ggcgtctcg 
      acgctactt cagtcaaca ttctggtgc cgaccatgg 
      gacttgtga gggctgctg ctgccatca cgcccgtcc 
      ttcctagcg aagaagtca actattcgg acgccggta 
      gtgaaacag acgaatcag actggtcag gtatcctaa 
      catatcaaa tattgggtg tgatatttc tgacggcga 
      tggcgttag ttcttctag cggctgtga gttatcaat 
      gcgccgcca cgtggaaat ggataagag acaaaacct 
      gcggcgacc cttcggacg ccgagacct ccatcaaca 
      agcaacaag ccgctatca gaatcagtc actctctac 
      cggacttgc aatcggagc ccatgaccc aggagtacg 
      tccaagagc acaaagact cgatggcca agaaggtcc 
      tgcgccaaa ccaccttgc ccatcgtgc ccaccaggc 
      accagacct tccaacggc aactgtccc atcggctgg 
      agatgccct cgaacgtcc agttcagca tctgcacgc 
      tggagcaca ggagcgact cgagtatcg ttgggctga 
      ttaagcccg agttgtata agaaagatt tggtccgcc 
      agtcgtccg ctgacagct ccgggggtc ccgagatgg 
      aatactgtg gcaaattgc atttctcgc tgcgatacg 
      acaaagacg tagaagggc ttgtcgtga gggtgttcg 
      agggccgcg atttgccca tcaaggact ccacgggca 
      gcagcgacc cctacataa aactctacc tcctgccgg 
      accggaaaa agaaattcc agacgaaag tccaccgga 
      aaaacctca atccggtgt ttaacgaga cgtttatat 
      tcagtgttc cctacgagg agctgcggc agcggtacc 
      tccagttct ccgtgtacg acttcgacc gcttctcgc 
      ggcacgacc tcatcgggc acgtcgtcc tcaagggcc 
      tcctggacg ccacggacc tgcagcagg agatcgagt 
      acacgatga acatcctct gcccgccac aggaaaaag 
      tcgatttgg gcgagctga tgctgtcgc tttgttatc 
      tgccgacgg ccggaagac tgaccatca cgataatta 
      aggggagga gtctcaaag cgatggata ttacgggaa 
      aatcagatc cttacgtta aagtgtatt tgctttgtc 
      aaggcaagc ggataaaga agaaaaaga cgtcggtga 
      agaaaaata cgctcaacc cggtgtata acgaggcgt 
      tggtctttg atgtcccgg ctgagaata tcgaggatg 
      tttccctca ttgttaaag tgatagact acgacagaa 
      tcggttcaa atgagctga tgggctgca cggcgatag 
      gttcaagtt tcataggaa tcggcaagg accactggt 
      tagaaatgc tggacaatc cccggtgcc ccgtggccc 
      aatggtacc ccctcctcg aatcgatac cgggccaca 
      tacccgcca acgactcca cccccctca gcctcagct 
      gcctcaaca ggtgaacga ctattccgc gacgtaaaa 
      acgaagaaa acaaacgtc tggtgctga actcatgaa 
      atggaatag cagatcaat ccctgccgt agattgtca 
      gtattcggt gttgcgaga caatttcca aaaagacaa 
      ttcgctatg tgtgtgaac aataagcaa caatgttgt 
      attgtagag ttattttcg gttttactt attgtattg 
      acaataccg atgcatatc agctccttg ttgtactga 
      gagatggcc gtttaagtg gaagatgta cgaaattag 
      taaaggcgt ttcggccta cacgagtac atcttccgg 
      gctcaaggc tcgccgcta gtatcttgc tctctgtta 
      cagtacttg tagatttat tatcgatct acgtgtctg 
      aatatcatg tgcatgttt gttatcagc ttgttaaaa 
      taagagaaa ttattttaa ataaatata tattgtact 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY