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Gene information
General information
TC007464 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4130144..4135136
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007464
    iBeetle screen iB_04334

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007464 RA
      MRRRVFSWI FIWTCLRLG RSEQLASNF TPTNDQKDM 
RGLFSPRMD YDQWKPLGR GDPLKNDPT YDYTRRCAN 
RTHLSRAKT KKPKSSSWE SPPRSPAPG PQSPTPGRT 
PSTHFSNSW TARNSPTRE TTGPTTPWG SSYFPPNFY 
PKNKNNLPK NPYTVLMPP PMITKKPAL SSPNPTTTD 
PTTTPATTA AKTEQVTVQ AANLVYHSS SVGDWKTET 
STNLTPNPA TNRLKPFRD EIVFQKPFQ LPESYIQIQ 
FAPSRHNQT LVAASSDHN INYVTPPPL VEEVMHKGQ 
VSDDNLDIS NTYVKIGKT EAALMGQPP PPPPMMVFS 
HVQHPLITQ TPLTMQTIQ SMQSMQPPP VTTSPVYTK 
STNPILEAL EKEEVTTER IETTTTVPT TTTTTSLTT 
DPLFKHYKQ PTEPLRGPL YLIIQGHSK VKTYGPSKQ 
FHGIRVQET NEISDDEVR DKRLLSKKE ETANSREAR 
SGKLQTLKH VIQTGLGAI DFSDVDRSD RVKETELHF 
GYEVGEGVT RETYHKGIV EEARKLHEE A
    CDS sequence
    • >TC007464 RA
      atgaggcga cgagtcttc tcttggatt ttcatctgg 
acttgttta cggctcgga cggagcgag caactcgcc 
agtaatttc actccgact aatgaccag aaagatatg 
agaggactt ttctccccg cggatggac tacgaccag 
tggaagcct ctgggccga ggagaccca ttaaaaaac 
gacccaacc tacgactac acccggcgt tgcgcaaac 
cggacccac ctctcccgg gcgaaaacc aaaaaaccg 
aaatcctcc tcctgggag tctcctcca agaagccca 
gcaccggga cctcaatcg ccgactccc ggaaggaca 
ccttcgact catttctca aattcgtgg acggcccga 
aattcacca accagagaa acaaccggc cccactacc 
ccatggggc tcgtcctac ttcccgccc aatttctac 
cccaaaaac aaaaacaac ctcccgaaa aacccctac 
accgtgcta atgccccca cccatgatc accaaaaaa 
cccgccctc tcctcgccc aaccccacc accaccgac 
cccaccacg accccggct acaaccgcg gctaaaacc 
gaacaagtg accgtccaa gcggcgaat ttagtgtac 
cattcaagc agtgtcggc gactggaag acggaaact 
tccaccaac ctaaccccg aacccggcc accaaccgg 
ttaaaaccg ttccgggac gaaatcgtc ttccagaaa 
cccttccaa ctgcctgaa agctacatc cagatccag 
ttcgccccc agtaggcac aaccagacg ttggtggcg 
gcgagcagc gaccataac attaactat gtgacgccg 
cccccactc gtggaggaa gtgatgcat aaagggcaa 
gtctcggac gataatttg gacatatcg aacacttac 
gtcaaaatt ggcaaaacc gaggctgct ctgatgggc 
cagccgccg ccgccccca cccatgatg gtcttctcg 
cacgtgcaa cacccgcta ataacgcag actccgctc 
acgatgcag accattcag agcatgcag agcatgcag 
ccgccccct gtcaccaca agcccggtt tacacgaaa 
tcaacgaat ccgattctg gaagcgttg gagaaggag 
gaagtgacc acggagagg attgaaacg accacgact 
gtgcccacg accaccacc accacgtcc ttgaccacg 
gaccccctg ttcaagcac tacaaacaa cccacggag 
cccctccgc ggtccgctc tacttgatc atccaggga 
cactccaaa gtgaagacg tacggaccc agcaaacag 
ttccatggg ataagggtg caggaaacc aacgagata 
tccgatgac gaggtccgc gacaagcgc ttgctttcc 
aagaaggag gaaactgca aattcaagg gaagcgaga 
tcggggaaa ttgcaaact ttgaaacat gtgatccag 
actggattg ggtgccatt gactttagt gacgtggat 
aggagcgat agggtgaaa gaaacggag ctgcacttc 
gggtacgaa gtgggggaa ggggtgacg cgggagact 
tatcataag ggaatcgtc gaagaggct agaaagctc 
cacgaggag gcttga
    mRNA sequence
    • >TC007464 RA
      catagagtg tttacgaac aacacacaa tcgaataat 
taacaatag tgttcgaat tctagttta aactaacat 
gtgctctac cctcagctt cgggtaatc gcctaattc 
atggacaag gagcaacca aaacactaa gatcatgag 
gcgacgagt cttctcttg gattttcat ctggacttg 
tttacggct cggacggag cgagcaact cgccagtaa 
tttcactcc gactaatga ccagaaaga tatgagagg 
acttttctc cccgcggat ggactacga ccagtggaa 
gcctctggg ccgaggaga cccattaaa aaacgaccc 
aacctacga ctacacccg gcgttgcgc aaaccggac 
ccacctctc ccgggcgaa aaccaaaaa accgaaatc 
ctcctcctg ggagtctcc tccaagaag cccagcacc 
gggacctca atcgccgac tcccggaag gacaccttc 
gactcattt ctcaaattc gtggacggc ccgaaattc 
accaaccag agaaacaac cggccccac taccccatg 
gggctcgtc ctacttccc gcccaattt ctaccccaa 
aaacaaaaa caacctccc gaaaaaccc ctacaccgt 
gctaatgcc cccacccat gatcaccaa aaaacccgc 
cctctcctc gcccaaccc caccaccac cgaccccac 
cacgacccc ggctacaac cgcggctaa aaccgaaca 
agtgaccgt ccaagcggc gaatttagt gtaccattc 
aagcagtgt cggcgactg gaagacgga aacttccac 
caacctaac cccgaaccc ggccaccaa ccggttaaa 
accgttccg ggacgaaat cgtcttcca gaaaccctt 
ccaactgcc tgaaagcta catccagat ccagttcgc 
ccccagtag gcacaacca gacgttggt ggcggcgag 
cagcgacca taacattaa ctatgtgac gccgccccc 
actcgtgga ggaagtgat gcataaagg gcaagtctc 
ggacgataa tttggacat atcgaacac ttacgtcaa 
aattggcaa aaccgaggc tgctctgat gggccagcc 
gccgccgcc cccacccat gatggtctt ctcgcacgt 
gcaacaccc gctaataac gcagactcc gctcacgat 
gcagaccat tcagagcat gcagagcat gcagccgcc 
ccctgtcac cacaagccc ggtttacac gaaatcaac 
gaatccgat tctggaagc gttggagaa ggaggaagt 
gaccacgga gaggattga aacgaccac gactgtgcc 
cacgaccac caccaccac gtccttgac cacggaccc 
cctgttcaa gcactacaa acaacccac ggagcccct 
ccgcggtcc gctctactt gatcatcca gggacactc 
caaagtgaa gacgtacgg acccagcaa acagttcca 
tgggataag ggtgcagga aaccaacga gatatccga 
tgacgaggt ccgcgacaa gcgcttgct ttccaagaa 
ggaggaaac tgcaaattc aagggaagc gagatcggg 
gaaattgca aactttgaa acatgtgat ccagactgg 
attgggtgc cattgactt tagtgacgt ggataggag 
cgatagggt gaaagaaac ggagctgca cttcgggta 
cgaagtggg ggaaggggt gacgcggga gacttatca 
taagggaat cgtcgaaga ggctagaaa gctccacga 
ggaggcttg atttgtgat ttaatgtta tctttgtgc 
tataatttt ttatttatt tttatttat aggcgtttt 
tgagttttt ctattgtac ttattttta ataaatgta 
ttgcgtgaa g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY