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Gene information
General information
TC007442 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4418072..4421120
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007442
    iBeetle screen iB_04329

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007442 RA
      MYEPVDRCV TTILTPSYE SKDFERMDK PPEKPPRKN 
      KLNKQLKEE SNEETKNNR MKPGDTCVD IDQINLEAN 
      NYKNKFTNA SVQTSPLIP YRLRFWPKN CDFKCAPED 
      SCCSKVGLV CCCRIISQW VLSQVGLTI VVVLWALLG 
      AFAFYKTEG PRELAQSEN LAKLQRNLS ANLASELEK 
      TPRENWKFT IDKYLQKHE TNLLEAVSA GFGEGGGGS 
      IWTYPGCIL FAVSLLTTL GFGAPVPRT SLGRGSAVL 
      FSAIGIPLH FLLILNMGN LGAVRLQQL AFGTSSDVP 
      TSERPRWLK WFPFFAILF YYLMGVVLF GFVRGRDPI 
      DCFMFPLDF TAAGGVATT YGHVRVLYA LYLEFAVTL 
      AALVLSLTQ ASATRGVVD IGLKLGLLT NT
    CDS sequence
    • >TC007442 RA
      atgtacgag ccggtggat cgatgtgtc accactatc 
      cttactcca agctatgag agtaaggat tttgagagg 
      atggacaag ccaccggag aagccgccg aggaaaaat 
      aagctaaat aaacagctg aaagaggag tcgaatgaa 
      gaaaccaaa aacaataga atgaaaccg ggtgacact 
      tgcgtcgac attgaccaa atcaatctc gaagccaac 
      aactacaag aacaaattc actaacgcc tccgtccaa 
      acttccccc cttattcca tacagactc aggttttgg 
      ccgaaaaac tgcgatttt aaatgtgct ccggaagac 
      agttgctgc agcaaagtg ggactggtg tgctgctgc 
      cgaatcata agtcaatgg gtgctgtcc caagtgggc 
      cttaccatc gtggtggtg ctatgggcc ttgctgggc 
      gccttcgcc ttttacaag accgaaggg cctcgcgaa 
      ctcgcacaa tccgaaaac ttagccaaa ctccaacgc 
      aatctctcc gcgaactta gccagcgaa ttggagaaa 
      acgccacgt gaaaactgg aaattcacg atcgataaa 
      tatttgcaa aagcacgaa acgaatctt ctggaggct 
      gtcagtgcc ggctttggc gaaggcggt ggaggaagc 
      atttggaca tatcctgga tgcatcctg ttcgccgtg 
      tctttattg acaacgctc gggttcggt gcccccgtg 
      ccccggacg tccctcggg cgcggctcc gccgtgctc 
      ttctcggcc atcgggatc cccctgcac ttcctcctc 
      attctcaac atgggcaac ctgggggcg gtgcgcctc 
      cagcagctc gcattcggc acttcctcc gacgtacct 
      acctccgag cgcccccga tggctcaaa tggttccct 
      ttcttcgcc attttattc tactatctc atgggggtt 
      gtgctcttc ggctttgta aggggccgc gaccccata 
      gactgtttc atgttcccg ttagatttc accgccgcc 
      gggggcgtc gccacaact tacggacac gttagagtc 
      ctttacgcg ttgtacctg gaatttgct gtcacgtta 
      gcggcccta gtcttgtct ctgacccag gcttccgca 
      acgaggggc gttgtcgac atcggcctc aaattgggg 
      ctcttgacg aacacttga
    mRNA sequence
    • >TC007442 RA
      aacattcac aaaaaaaca caatattgg cgaaaaaat 
      gagcacaat tcgggcgtt ctattccca aaaggttaa 
      ttttagata atcgaaatg tacgagccg gtggatcga 
      tgtgtcacc actatcctt actccaagc tatgagagt 
      aaggatttt gagaggatg gacaagcca ccggagaag 
      ccgccgagg aaaaataag ctaaataaa cagctgaaa 
      gaggagtcg aatgaagaa accaaaaac aatagaatg 
      aaaccgggt gacacttgc gtcgacatt gaccaaatc 
      aatctcgaa gccaacaac tacaagaac aaattcact 
      aacgcctcc gtccaaact tcccccctt attccatac 
      agactcagg ttttggccg aaaaactgc gattttaaa 
      tgtgctccg gaagacagt tgctgcagc aaagtggga 
      ctggtgtgc tgctgccga atcataagt caatgggtg 
      ctgtcccaa gtgggcctt accatcgtg gtggtgcta 
      tgggccttg ctgggcgcc ttcgccttt tacaagacc 
      gaagggcct cgcgaactc gcacaatcc gaaaactta 
      gccaaactc caacgcaat ctctccgcg aacttagcc 
      agcgaattg gagaaaacg ccacgtgaa aactggaaa 
      ttcacgatc gataaatat ttgcaaaag cacgaaacg 
      aatcttctg gaggctgtc agtgccggc tttggcgaa 
      ggcggtgga ggaagcatt tggacatat cctggatgc 
      atcctgttc gccgtgtct ttattgaca acgctcggg 
      ttcggtgcc cccgtgccc cggacgtcc ctcgggcgc 
      ggctccgcc gtgctcttc tcggccatc gggatcccc 
      ctgcacttc ctcctcatt ctcaacatg ggcaacctg 
      ggggcggtg cgcctccag cagctcgca ttcggcact 
      tcctccgac gtacctacc tccgagcgc ccccgatgg 
      ctcaaatgg ttccctttc ttcgccatt ttattctac 
      tatctcatg ggggttgtg ctcttcggc tttgtaagg 
      ggccgcgac cccatagac tgtttcatg ttcccgtta 
      gatttcacc gccgccggg ggcgtcgcc acaacttac 
      ggacacgtt agagtcctt tacgcgttg tacctggaa 
      tttgctgtc acgttagcg gccctagtc ttgtctctg 
      acccaggct tccgcaacg aggggcgtt gtcgacatc 
      ggcctcaaa ttggggctc ttgacgaac acttgagct 
      gtgtaggaa gtgccttag tagtcgcgc tgcactttg 
      gtttgatat acatatcac ttccggggg gccggagag 
      taagggtgc gtcagggcg attgaaaaa tattaaagt 
      aaccgaagg ttttattga gacaagtaa agtgtgtag 
      ggggttgct tagaggtat gtaccccac acttttttt 
      ccataaaaa atatgcata aaagacacc ccataatta 
      cagttggtt taaattaaa atttctaat aaacaagtc 
      acaaagtca atgactcgt gtgggtaat tcgaatgtc 
      tgtgatgca acatcgcgc tgatgtagc cttatcagg 
      actgcctta atgcgaatt aaacaaaac tttagatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY