Status
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Gene information
General information
TC007441 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4477244..4479470
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007441
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04328

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007441 RA
      MLCTNAAFF TGKSDKKAN PWGLQEQSW SWTWCNCFK 
      RKIAPVDTP IKYEEGTQT KLETPQLTP IRLNSTQSF 
      TQQIPPPSS GSESSGYFT PPAEHHLKE EEAEEENNN 
      LQEPNFQIV ESENSEVHE VEDIRQSSA SSAEVREVS 
      FDEVDKQEI KRFSSLEDV DFSENRHFS EPRSKSRAE 
      PSLKSSLPE ISQRKNNSA PPLELPLVP YKSPEKTPI 
      KTQLPGSVA STETTPKKL SGQSPRRLS RRNTSLKRT 
      GKSPKTPAI SRPKEILNQ ALAQMESCD WETVIQGLN 
      SLTRLSHTT PDVLEPQMH QICITLARN IKNLRSQVA 
      RCACHASSE LFNSCKKGL EMELEELAT PLLHRTADT 
      NKFLRSDAN SALDAMALN MSVPRAVAV IANKGSTHQ 
      NGVVRGAAT RLMSDLAMR LGAEKVFQF PKETRDKFL 
      LVGANGLTE GSLETRRHA KALMSALVG HGMFHKVLV 
      EAVPQHTLR HIAKAIAGL K
    CDS sequence
    • >TC007441 RA
      atgttgtgc acgaacgcg gcgtttttc actgggaaa 
      agtgacaaa aaagccaat ccgtggggc ctccaagag 
      cagagctgg tcatggaca tggtgtaac tgttttaag 
      agaaaaatc gcaccggtt gatacgccc ataaagtat 
      gaagagggc acccaaacg aagctggaa actccccaa 
      ttgaccccg attcggttg aactctact caaagtttc 
      acccaacaa attcctccc ccgtcctca gggtccgaa 
      tcctcgggg tatttcacg ccaccggcg gaacaccac 
      ttgaaggag gaggaagcc gaagaggaa aataacaat 
      ttgcaagaa cctaacttc cagatagtg gagtctgaa 
      aattcggaa gttcacgaa gttgaagat attagacaa 
      tcctcggca agttcagcc gaagtccgt gaagtgtct 
      ttcgacgaa gtcgacaaa caagaaatc aaacgtttc 
      agttcgttg gaggacgtc gatttcagc gaaaatcgt 
      cacttttcc gaacctagg agcaaatcc cgggctgaa 
      ccttcactc aaatccagc ttacccgaa atttcgcaa 
      cgcaagaac aactccgca cccccactt gaactcccc 
      ttagtgcct tacaaatcc cccgaaaaa accccaatt 
      aaaacccaa ctcccgggt tcagtagcc tccaccgag 
      accacaccc aagaaacta tcgggtcaa tcaccaaga 
      cgtctctca cggcgcaac accagcctg aaacggacg 
      ggaaaatcg cccaaaacc ccggcaatc agccggccg 
      aaggaaatc ctaaaccag gctttggcc caaatggag 
      agctgtgac tgggagacg gtgatccag ggcttaaac 
      tccctcacc cgcctctca cacacaacc cccgacgtc 
      ctggagccg caaatgcac cagatttgc atcactctg 
      gcccgaaat atcaaaaat ttgcggtcg caagtcgcc 
      aggtgtgcg tgtcacgcc tcgagcgag cttttcaat 
      tcctgcaaa aagggccta gaaatggag ttggaggaa 
      ttggcgacg cctctcctc caccgcacc gccgacacg 
      aacaaattc ctgcgcagt gacgcgaac tcggccctg 
      gacgccatg gcccttaac atgtcggtg ccaagggcc 
      gtggctgtg attgccaac aaagggtcc acgcaccaa 
      aacggggtt gtcagaggg gcggcgacg agacttatg 
      agcgatttg gcgatgagg ctcggtgcc gagaaggtt 
      tttcagttc ccgaaggag accagggat aagtttttg 
      ttggtgggc gcgaatggc ttgacggag gggagtttg 
      gagacgcga cggcatgcc aaagcgctg atgtcggcg 
      cttgtgggg cacgggatg tttcacaag gttttggtt 
      gaggcggtg cctcaacac acgctgagg cacattgcc 
      aaggcgata gccgggttg aagtga
    mRNA sequence
    • >TC007441 RA
      ccgccgcat tatgttaat aaattatcg attccccat 
      ttccccaaa catcttgcc ctaattaat aatccagcc 
      cctcatacc tcgacctat taaggtcac agcgatgat 
      tcgctcgca tgtcccgtc ataattaca cccatcttg 
      aacccattt acggaccca tcgaactgt cgacgcacc 
      gttgaatcg attttcctt ctagcccgt gtatgtggg 
      agcgcatta attacaata ataatcgat cgttttctg 
      catacgttt aaaacggtt actccgccg cgaaatcat 
      accgtggaa tagttccgg gaattaacc ccattgcag 
      acccttatc accctttga tatcgatac acgacgcat 
      tctgccatc gacctatga ccgtctgct ttgaggcca 
      cgcggaggg gcagaggtg gtcaagaca atcaatgcg 
      gccggaaaa ttagtcggg aaacacttt caagtggga 
      aatgttgtg cacgaacgc ggcgttttt cactgggaa 
      aagtgacaa aaaagccaa tccgtgggg cctccaaga 
      gcagagctg gtcatggac atggtgtaa ctgttttaa 
      gagaaaaat cgcaccggt tgatacgcc cataaagta 
      tgaagaggg cacccaaac gaagctgga aactcccca 
      attgacccc gattcggtt gaactctac tcaaagttt 
      cacccaaca aattcctcc cccgtcctc agggtccga 
      atcctcggg gtatttcac gccaccggc ggaacacca 
      cttgaagga ggaggaagc cgaagagga aaataacaa 
      tttgcaaga acctaactt ccagatagt ggagtctga 
      aaattcgga agttcacga agttgaaga tattagaca 
      atcctcggc aagttcagc cgaagtccg tgaagtgtc 
      tttcgacga agtcgacaa acaagaaat caaacgttt 
      cagttcgtt ggaggacgt cgatttcag cgaaaatcg 
      tcacttttc cgaacctag gagcaaatc ccgggctga 
      accttcact caaatccag cttacccga aatttcgca 
      acgcaagaa caactccgc acccccact tgaactccc 
      cttagtgcc ttacaaatc ccccgaaaa aaccccaat 
      taaaaccca actcccggg ttcagtagc ctccaccga 
      gaccacacc caagaaact atcgggtca atcaccaag 
      acgtctctc acggcgcaa caccagcct gaaacggac 
      gggaaaatc gcccaaaac cccggcaat cagccggcc 
      gaaggaaat cctaaacca ggctttggc ccaaatgga 
      gagctgtga ctgggagac ggtgatcca gggcttaaa 
      ctccctcac ccgcctctc acacacaac ccccgacgt 
      cctggagcc gcaaatgca ccagatttg catcactct 
      ggcccgaaa tatcaaaaa tttgcggtc gcaagtcgc 
      caggtgtgc gtgtcacgc ctcgagcga gcttttcaa 
      ttcctgcaa aaagggcct agaaatgga gttggagga 
      attggcgac gcctctcct ccaccgcac cgccgacac 
      gaacaaatt cctgcgcag tgacgcgaa ctcggccct 
      ggacgccat ggcccttaa catgtcggt gccaagggc 
      cgtggctgt gattgccaa caaagggtc cacgcacca 
      aaacggggt tgtcagagg ggcggcgac gagacttat 
      gagcgattt ggcgatgag gctcggtgc cgagaaggt 
      ttttcagtt cccgaagga gaccaggga taagttttt 
      gttggtggg cgcgaatgg cttgacgga ggggagttt 
      ggagacgcg acggcatgc caaagcgct gatgtcggc 
      gcttgtggg gcacgggat gtttcacaa ggttttggt 
      tgaggcggt gcctcaaca cacgctgag gcacattgc 
      caaggcgat agccgggtt gaagtgagg gcacttctt 
      ccattgtgt tgtaggggt agagattta agtgtttat 
      ggtggggtt tttatttat ataaaattt atgacgaaa 
      taaaaatta ctgaaaaaa
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