Status
Please wait ...
Gene information
General information
TC007438 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4496832..4498682
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007438
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04327

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007438 RA
      MFRRLYLTQ CVAKRFIQR RPEVVKERQ TPHTLLWKT 
      IENTKIGSP QEWKDLRNE ILNSVKSIT PANIDAVTV 
      NVCTINKKY NLALSYLEF LRSENIELN LATLGNYLK 
      LLYAKGLEE QLSPKDEEK ILLLYEKIR KEYPVLDQS 
      TLNSIVQAL SLTSQWKQC LGLLDEIKV TGTINAHAS 
      CALIRAAFL HNEDALAWK LLNEVIESE KTVHSIVYS 
      TYFKRIGKK SDKIAQIEK MFNFMHDKG LKIESNQIE 
      EFAEALKSH KMLVRSSAI NYKGVCKSC NSKLAQFHL 
      TQSEFDEIK QKIIDNVII GKDVYARTT AQELSKFKN 
      FVSKMPKFD VVIDGLNVA YSAGLKQPA QVTSGLVQS 
      VVAHFVDQG KEVLLLGRS HMNRWPRNN WDYVKENAT 
      IFLTENLSQ DDPYLLYCA LHSGKDTII VTKDLMRGH 
      KFLLKDVRL KILFDRWLS QRQYQLVFA KEGGDCTFK 
      VTRAVVMQR AHASGIPKH PRILSIFAR FFIAETAKL 
      YAFSPKERT LLAHSVQIG EQICDE
    CDS sequence
    • >TC007438 RA
      atgtttaga aggctttat ctaactcag tgtgtggca 
      aaaaggttt attcagaga cgtcctgaa gtggtgaaa 
      gagcgccaa acaccgcac accctcctt tggaaaact 
      atcgagaat acgaaaatt ggttcacct caggaatgg 
      aaagacctg aggaatgaa attctgaac tctgttaaa 
      tctataact ccagcaaac attgatgca gttaccgta 
      aatgtgtgt acaataaac aaaaaatat aacttagct 
      ttgagttac ttggagttc cttagatcg gagaatatt 
      gaattaaat ttagcaacg ttagggaat tatttgaaa 
      ctgttatat gcgaaagga cttgaggaa caattgtcg 
      cctaaagat gaagagaaa attttgtta ttgtatgag 
      aagataagg aaagagtat cctgtttta gaccaaagt 
      actttaaat agcatcgtc caagcgtta tctttaact 
      agtcagtgg aagcagtgc ttaggtctt ttagatgaa 
      ataaaagta actggtacc attaacgct cacgcctct 
      tgtgcctta atcagagct gctttttta cacaacgaa 
      gacgccttg gcgtggaaa ttactcaac gaggtgata 
      gaaagcgaa aaaacggtt cactcaatc gtctattca 
      acttacttc aaaagaatc ggaaaaaaa tcagataaa 
      atcgcccaa atcgaaaaa atgttcaat tttatgcac 
      gacaaaggc ctaaaaatc gagtcaaac caaatcgag 
      gaattcgcc gaggcccta aagtcccac aaaatgctc 
      gtgcgttcg tctgccata aattacaaa ggcgtgtgt 
      aaaagctgc aactctaaa ctcgcccag ttccacttg 
      acccagtcc gaattcgac gaaatcaag caaaaaatc 
      atcgataac gtaatcatc ggcaaagac gtctatgcc 
      cgaaccacg gcacaagaa ttatcaaaa ttcaaaaac 
      tttgtctcg aaaatgccg aaatttgac gtcgtaatc 
      gacggcttg aacgtcgct tattcggca gggttgaaa 
      caaccagca caagtcacg tccgggctg gtccagtca 
      gtagtggcc cattttgtg gatcaaggc aaggaggtg 
      ctccttctg gggcgcagt cacatgaac aggtggccg 
      cgcaacaac tgggactac gtcaaggaa aacgcgacg 
      attttcctc acggaaaat ctgtctcaa gacgatccg 
      tacctcctc tactgtgct ttacattcg ggcaaagac 
      accataatt gtgaccaaa gatctgatg agaggccac 
      aagttcctg ctaaaggac gtccgattg aaaatttta 
      ttcgaccgc tggttgagt caaaggcaa taccagctg 
      gttttcgcc aaagagggc ggggattgt acttttaag 
      gtaacgagg gctgtcgtt atgcaacga gcgcatgcg 
      agtggcata cctaaacac ccgagaatt ttaagcatt 
      ttcgctcgt ttttttatt gcagaaacc gccaaacta 
      tacgcattt agcccaaaa gaacggacc cttctggca 
      cattccgtg caaatcgga gaacaaatt tgtgacgaa 
      taa
    mRNA sequence
    • >TC007438 RA
      agctgccag agttgacac tcaaaaaaa tcttttttt 
      cacttaaat gtgtctttt acgatgttt agaaggctt 
      tatctaact cagtgtgtg gcaaaaagg tttattcag 
      agacgtcct gaagtggtg aaagagcgc caaacaccg 
      cacaccctc ctttggaaa actatcgag aatacgaaa 
      attggttca cctcaggaa tggaaagac ctgaggaat 
      gaaattctg aactctgtt aaatctata actccagca 
      aacattgat gcagttacc gtaaatgtg tgtacaata 
      aacaaaaaa tataactta gctttgagt tacttggag 
      ttccttaga tcggagaat attgaatta aatttagca 
      acgttaggg aattatttg aaactgtta tatgcgaaa 
      ggacttgag gaacaattg tcgcctaaa gatgaagag 
      aaaattttg ttattgtat gagaagata aggaaagag 
      tatcctgtt ttagaccaa agtacttta aatagcatc 
      gtccaagcg ttatcttta actagtcag tggaagcag 
      tgcttaggt cttttagat gaaataaaa gtaactggt 
      accattaac gctcacgcc tcttgtgcc ttaatcaga 
      gctgctttt ttacacaac gaagacgcc ttggcgtgg 
      aaattactc aacgaggtg atagaaagc gaaaaaacg 
      gttcactca atcgtctat tcaacttac ttcaaaaga 
      atcggaaaa aaatcagat aaaatcgcc caaatcgaa 
      aaaatgttc aattttatg cacgacaaa ggcctaaaa 
      atcgagtca aaccaaatc gaggaattc gccgaggcc 
      ctaaagtcc cacaaaatg ctcgtgcgt tcgtctgcc 
      ataaattac aaaggcgtg tgtaaaagc tgcaactct 
      aaactcgcc cagttccac ttgacccag tccgaattc 
      gacgaaatc aagcaaaaa atcatcgat aacgtaatc 
      atcggcaaa gacgtctat gcccgaacc acggcacaa 
      gaattatca aaattcaaa aactttgtc tcgaaaatg 
      ccgaaattt gacgtcgta atcgacggc ttgaacgtc 
      gcttattcg gcagggttg aaacaacca gcacaagtc 
      acgtccggg ctggtccag tcagtagtg gcccatttt 
      gtggatcaa ggcaaggag gtgctcctt ctggggcgc 
      agtcacatg aacaggtgg ccgcgcaac aactgggac 
      tacgtcaag gaaaacgcg acgattttc ctcacggaa 
      aatctgtct caagacgat ccgtacctc ctctactgt 
      gctttacat tcgggcaaa gacaccata attgtgacc 
      aaagatctg atgagaggc cacaagttc ctgctaaag 
      gacgtccga ttgaaaatt ttattcgac cgctggttg 
      agtcaaagg caataccag ctggttttc gccaaagag 
      ggcggggat tgtactttt aaggtaacg agggctgtc 
      gttatgcaa cgagcgcat gcgagtggc atacctaaa 
      cacccgaga attttaagc attttcgct cgttttttt 
      attgcagaa accgccaaa ctatacgca tttagccca 
      aaagaacgg acccttctg gcacattcc gtgcaaatc 
      ggagaacaa atttgtgac gaataaaac gtactggtt 
      gtgtgtgca aaacaacaa ttccggtaa ataataaat 
      ttattcata atgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY