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Gene information
General information
TC007427 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4677132..4708090
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007427
    iBeetle screen iB_04326

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection


    Adult morphology
    motility decreased - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007427 RA
      MDRRLFSLV CCLFTVNFV SSDGPVFVN PSSVSLPPA 
      VNQGNYVTQ TVYGFLDFT TTIGNTVMV FSPQSAAVS 
      ESPKEESSN SVIDTSPIA KKEPVTIKP SKTEAKIIS 
      SKVEVKTSS QDKPSTKVS PSIITKVEV VSGPPVLSS 
      KVEIVTSSQ KAISSVVSV KTEEEPAVI LGNNIAEPE 
      YDFLSRQPS EVVEETYKV INLKPSSKF HLKPRTSEP 
      KNKRPDSLH PTGLVTKLG GTVVKDGTT TIHETSVIG 
      TYISGKYAQ VLQSTSHIH NPQKAKISP SPTLRILKT 
      AAPSLGKGK HHRHLEPTP AGSIEETIV AAESSNSIK 
      STRKPAGPT FKPRFKNRQ KEDEEESEE VQHAKKKSQ 
      RTRNQARNS NEEATTTSN KRYQNRRKQ QRPSTEPSS 
      SGGYSRRGY KPKVQTSTV EQISASTSL YKFKLNRSP 
      GRWQYKTTP KPRVTIRKQ NDDNDSGNE TLGTTPTTL 
      QDVSPALND VVTPQARSD DVDGLEGSE SIASIIDDQ 
      SSTENKLDT PFPLETLKV EISTPPDFK DIYYEIATI 
      KSPFTFQAG NVKNTRYIT VTSTFEKSL VNTPEATIS 
      PSGSEPLTE NILATSSNY KDNNFLDSS VATLPPIYL 
      ASDMQTPPL ETLTETFST TQTMLKTHI LPVIHSGNE 
      TATSTLVQT YLITRLVTA TKTLPPMEA YHFIPSKTL 
      KEFNSHLDE AGSELHLEL EFGDQNDQD DEGQLKRSF 
      PPDLDLAKV GSDFDISDV DKSKLPEIQ KAKTGKSKP 
      VLSEPQTPA LNPDQLQQL ALLRLLNPA AAQGQVITT 
      SKPVLKIET VYESHVIPV VNGGNTVLS TLSKVLGTV 
      TKTDFEFGT STLAPPTLP IPPIPPVNP LFPAAQFAV 
      TSSPVVQST LVTQTDSKV LKLTFGAKT AYTTIFSTK 
      VVPSLVTTY MTASVPVQP TAFPGFFPA PYPGYPFVG
    CDS sequence
    • >TC007427 RA
      atggatcgc cgcctcttc tccctggtt tgctgctta 
      tttacggtg aatttcgtt tcatcggat ggtcccgtc 
      ttcgtcaat ccctcgtca gtctccctc cccccagca 
      gttaatcag ggaaattac gtcactcaa actgtctat 
      ggcttcctc gactttacc actacaatc ggaaacact 
      gtcatggtt ttctcgcca caaagcgcc gctgtttca 
      gaatcgcca aaagaagaa tcctcgaac agcgtaatc 
      gatacgagt ccaatcgcg aaaaaagaa ccagtcaca 
      ataaaaccg agcaagact gaagccaaa ataatttcg 
      agtaaagtc gaagttaag acctcaagt caggacaaa 
      ccgagtacg aaagtttcc ccgagtatt ataacaaaa 
      gtggaggtc gtgagtggt cctccggtg ctttccagt 
      aaagtcgag attgtcaca agttcgcaa aaggcgatt 
      agtagcgtt gtatcggtt aaaaccgaa gaggagccg 
      gcggttatc ctcggcaac aacatcgcc gagcccgag 
      tatgacttc ctgtcccgt cagccgtcc gaagttgtt 
      gaagaaacc tacaaagtc atcaacctg aaacccagc 
      tcaaagttc catctcaag cctcggact tctgagcca 
      aaaaacaaa cgtccggac tcattacac ccgacggga 
      ttagtcacg aaattagga ggaactgtc gtaaaggac 
      ggcacgacg actatccac gagactagc gtgattggg 
      acctacatc tctggaaaa tacgcccaa gtcctccag 
      agcacctcg cacatccac aatccgcag aaagccaag 
      atcagtccg tcgccgacg ttgcgaatt ctcaaaaca 
      gccgcccct tcgttgggg aaaggcaag catcatcgg 
      catttggag ccaactccc gcgggctcc atagaggag 
      acgattgta gcggcggaa tcgtcgaat tccatcaaa 
      tcgacgagg aagccggca gggcccact ttcaagccg 
      cgcttcaaa aacagacag aaggaggac gaggaggag 
      agcgaggaa gtgcaacat gccaagaag aaatcgcaa 
      cggacacgg aaccaagcg aggaattcc aatgaagaa 
      gcaacgacc acgtcgaac aagcgctac cagaaccgc 
      cgcaagcag cagcgcccc agcaccgag ccctcgagc 
      tcggggggc tacagccgc cgcggctac aagcccaaa 
      gtgcagacc tcgaccgtg gagcaaatc tccgcgtct 
      acaagcctc tacaagttc aaattgaat cgctctcct 
      ggcagatgg caatacaag acgacaccc aagcctcgc 
      gttaccatc aggaagcaa aacgacgac aatgactcc 
      ggtaacgag acgctcgga acgacgccg acgacgctg 
      caagacgtg tctccggca ctcaacgac gtcgttacg 
      ccccaagcg aggtccgac gacgtcgac ggactcgaa 
      ggatccgaa tcaattgct tcaattatt gacgatcaa 
      agctccacc gaaaataaa ctcgacacg ccgttcccg 
      ctcgaaacg ctcaaggtg gaaatttcc acgccgccg 
      gattttaaa gatatctat tacgagatt gccaccatc 
      aagtcaccc ttcaccttc caggccggc aacgtgaaa 
      aacacccgt tacataacc gtcacgtct acgtttgaa 
      aaaagtctg gtgaacact cccgaagcc acaatctcc 
      ccgtcaggc agcgagcca ttaacggaa aatatttta 
      gccacttca agcaattac aaagacaat aatttcctg 
      gattcgagt gttgccacg ttaccaccg atttatctg 
      gcatccgac atgcaaacc ccgcccttg gaaacactc 
      acagagaca ttcagcacg actcaaacc atgctcaag 
      actcatatc ctacccgtc attcacagc ggcaacgaa 
      acagccact tcaacactc gttcaaacg tatctaata 
      acgagatta gtgacggca acgaagact ttaccacct 
      atggaagct tatcatttc attccgagc aaaactctg 
      aaagagttc aacagtcat ttggatgag gcggggtca 
      gagctccac ttagagttg gagtttggc gaccaaaac 
      gatcaggac gatgaagga caattgaaa aggagtttc 
      cccccggat ctggatttg gctaaagtt ggatccgat 
      tttgatatt tccgacgtc gacaaatcc aaacttccg 
      gaaatccag aaagcgaaa acgggaaaa tcgaaaccg 
      gttttgagc gaaccccaa accccggcc ttgaacccc 
      gaccagctg caacaattg gcgcttttg aggctcttg 
      aacccggcg gcggcccaa ggccaggtc atcacgacc 
      tcgaaacca gtcctcaaa attgaaacc gtctacgaa 
      tcccacgtt attccggtc gtcaacggg ggaaacacc 
      gtcttgagc acgctctcc aaggtcctg gggaccgta 
      acgaagacg gatttcgag ttcgggacg agcaccctg 
      gcgcccccg acgctgcca atcccgccg atccctccg 
      gtgaatccc ctgtttccc gcggctcaa ttcgccgtc 
      acttcgtcg cccgtggtc caaagcact ttggttacg 
      caaactgat agcaaggtg ctcaagctg actttcggg 
      gctaagacg gcttatacg acgattttc tccacgaaa 
      gtcgtaccc agcttggta acgacgtat atgacggcg 
      tcggtgccc gtacaaccc acggctttc ccgggattc 
      ttcccggcg ccctatccc ggatatccc ttcgtgggc 
      tga
    mRNA sequence
    • >TC007427 RA
      ccagtagac ttcaagacg ttcacttct cccgctggt 
      ttgagcttt cccccgtgc tcctccacc cgactctgg 
      tccccggat aatgtgcgt ggtgtagaa tcgcaacga 
      tggatcgcc gcctcttct ccctggttt gctgcttat 
      ttacggtga atttcgttt catcggatg gtcccgtct 
      tcgtcaatc cctcgtcag tctccctcc ccccagcag 
      ttaatcagg gaaattacg tcactcaaa ctgtctatg 
      gcttcctcg actttacca ctacaatcg gaaacactg 
      tcatggttt tctcgccac aaagcgccg ctgtttcag 
      aatcgccaa aagaagaat cctcgaaca gcgtaatcg 
      atacgagtc caatcgcga aaaaagaac cagtcacaa 
      taaaaccga gcaagactg aagccaaaa taatttcga 
      gtaaagtcg aagttaaga cctcaagtc aggacaaac 
      cgagtacga aagtttccc cgagtatta taacaaaag 
      tggaggtcg tgagtggtc ctccggtgc tttccagta 
      aagtcgaga ttgtcacaa gttcgcaaa aggcgatta 
      gtagcgttg tatcggtta aaaccgaag aggagccgg 
      cggttatcc tcggcaaca acatcgccg agcccgagt 
      atgacttcc tgtcccgtc agccgtccg aagttgttg 
      aagaaacct acaaagtca tcaacctga aacccagct 
      caaagttcc atctcaagc ctcggactt ctgagccaa 
      aaaacaaac gtccggact cattacacc cgacgggat 
      tagtcacga aattaggag gaactgtcg taaaggacg 
      gcacgacga ctatccacg agactagcg tgattggga 
      cctacatct ctggaaaat acgcccaag tcctccaga 
      gcacctcgc acatccaca atccgcaga aagccaaga 
      tcagtccgt cgccgacgt tgcgaattc tcaaaacag 
      ccgcccctt cgttgggga aaggcaagc atcatcggc 
      atttggagc caactcccg cgggctcca tagaggaga 
      cgattgtag cggcggaat cgtcgaatt ccatcaaat 
      cgacgagga agccggcag ggcccactt tcaagccgc 
      gcttcaaaa acagacaga aggaggacg aggaggaga 
      gcgaggaag tgcaacatg ccaagaaga aatcgcaac 
      ggacacgga accaagcga ggaattcca atgaagaag 
      caacgacca cgtcgaaca agcgctacc agaaccgcc 
      gcaagcagc agcgcccca gcaccgagc cctcgagct 
      cggggggct acagccgcc gcggctaca agcccaaag 
      tgcagacct cgaccgtgg agcaaatct ccgcgtcta 
      caagcctct acaagttca aattgaatc gctctcctg 
      gcagatggc aatacaaga cgacaccca agcctcgcg 
      ttaccatca ggaagcaaa acgacgaca atgactccg 
      gtaacgaga cgctcggaa cgacgccga cgacgctgc 
      aagacgtgt ctccggcac tcaacgacg tcgttacgc 
      cccaagcga ggtccgacg acgtcgacg gactcgaag 
      gatccgaat caattgctt caattattg acgatcaaa 
      gctccaccg aaaataaac tcgacacgc cgttcccgc 
      tcgaaacgc tcaaggtgg aaatttcca cgccgccgg 
      attttaaag atatctatt acgagattg ccaccatca 
      agtcaccct tcaccttcc aggccggca acgtgaaaa 
      acacccgtt acataaccg tcacgtcta cgtttgaaa 
      aaagtctgg tgaacactc ccgaagcca caatctccc 
      cgtcaggca gcgagccat taacggaaa atattttag 
      ccacttcaa gcaattaca aagacaata atttcctgg 
      attcgagtg ttgccacgt taccaccga tttatctgg 
      catccgaca tgcaaaccc cgcccttgg aaacactca 
      cagagacat tcagcacga ctcaaacca tgctcaaga 
      ctcatatcc tacccgtca ttcacagcg gcaacgaaa 
      cagccactt caacactcg ttcaaacgt atctaataa 
      cgagattag tgacggcaa cgaagactt taccaccta 
      tggaagctt atcatttca ttccgagca aaactctga 
      aagagttca acagtcatt tggatgagg cggggtcag 
      agctccact tagagttgg agtttggcg accaaaacg 
      atcaggacg atgaaggac aattgaaaa ggagtttcc 
      ccccggatc tggatttgg ctaaagttg gatccgatt 
      ttgatattt ccgacgtcg acaaatcca aacttccgg 
      aaatccaga aagcgaaaa cgggaaaat cgaaaccgg 
      ttttgagcg aaccccaaa ccccggcct tgaaccccg 
      accagctgc aacaattgg cgcttttga ggctcttga 
      acccggcgg cggcccaag gccaggtca tcacgacct 
      cgaaaccag tcctcaaaa ttgaaaccg tctacgaat 
      cccacgtta ttccggtcg tcaacgggg gaaacaccg 
      tcttgagca cgctctcca aggtcctgg ggaccgtaa 
      cgaagacgg atttcgagt tcgggacga gcaccctgg 
      cgcccccga cgctgccaa tcccgccga tccctccgg 
      tgaatcccc tgtttcccg cggctcaat tcgccgtca 
      cttcgtcgc ccgtggtcc aaagcactt tggttacgc 
      aaactgata gcaaggtgc tcaagctga ctttcgggg 
      ctaagacgg cttatacga cgattttct ccacgaaag 
      tcgtaccca gcttggtaa cgacgtata tgacggcgt 
      cggtgcccg tacaaccca cggctttcc cgggattct 
      tcccggcgc cctatcccg gatatccct tcgtgggct 
      gagtgacgt gcggtcgca gtggacgat ccttgtaca 
      gttttgtgt aaataagtg tgagggaaa tcggtgagg 
      actgaggcg gtatttata atcttcatg tgcatttac 
      cactacttg taaatattt taattagga gtgtatttt 
      tatattgta aagatggac aataaaatt tacgaaata 
      ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY