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Gene information
General information
TC007419 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4763085..4765454
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007419
    iBeetle screen iB_04325

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007419 RA
      MIDLLLDSQ HHPFLFLAG RPQGTQQLV NETHKQLEF 
      QRRQQEQML QQQQKLQEL QGQITAQYA SGVGPQGLM 
      FLPFLEQLR GLQPPKSLA NHMLPPHQV PSWISATAH 
      LAHMSASSD KRSPTPQRT PSPPAPPTD PDAPLNLSK 
      PKSSSSGSA GSSPQSAPA GLQTFNDQP VAATAPKLL 
      PPSLVMPRA FLPYAGLPP QFSPLPASV DRKGKDEKQ 
      PHFPMHMYG LPAPPHMGG RPKDDGGIK DETDFMAAC 
      HMWGGPDPN FKLDENSEK AKIIRQQPK RDSENKPHI 
      KRPMNAFMV WAKDERRKI LKACPDMHN SNISKILGA 
      RWKSMSNAE KQPYYEEQS RLSKLHMEK HPDYRYRPR 
      PKRTCIVDG KKMRISEYK MLMRQRRQE MRQLWCRDG 
      ASDMNFSLS GADLGAATA TRAGSPMSM VNGAGPSAE 
      GFYYPEGAS PDALNFAQE HAMMHDSSP RNEDD
    CDS sequence
    • >TC007419 RA
      atgatcgac ctcctgctg gattcacaa caccacccg 
      ttcttgttc ttagctggt cgacctcaa ggcacccaa 
      caattagtc aatgaaacc cacaaacaa ttggagttc 
      caaagacgg cagcaggaa caaatgctg cagcagcaa 
      caaaaattg caagaactc caaggccag attacggca 
      cagtacgcc agtggcgtg gggcctcaa ggacttatg 
      ttcctcccg tttttggag caactgcga ggactccag 
      ccccccaaa tcccttgcc aatcacatg ctgcccccg 
      caccaagtc cccagctgg atctccgcg accgcccat 
      ctggcccac atgtcggcc agttcggac aagaggtcg 
      cccaccccc caacggacc ccgtcgccc ccagcgccg 
      ccgacggac cccgacgcc ccgctcaac ctctcgaaa 
      ccgaaatcg tcgagttcc ggttcggcg ggatcgagt 
      ccgcaaagt gccccggcc ggcttgcag actttcaac 
      gatcagccg gtggcggcc accgccccc aaacttcta 
      cctcccagt ttagtaatg ccgcgggcg ttcttgccc 
      tacgccgga ctgccccca cagtttagt ccactgccg 
      gcgagcgtc gaccggaag gggaaagat gagaagcaa 
      cctcatttc ccgatgcat atgtacggc ctgccggcg 
      cccccgcac atggggggc aggcccaag gacgacggc 
      ggcatcaag gatgagacc gacttcatg gcggcgtgt 
      cacatgtgg ggcggcccc gacccgaac ttcaagctg 
      gacgaaaac tccgaaaag gcgaaaatc atccgccag 
      caaccgaaa cgcgactcc gagaacaaa ccccacatc 
      aagcgcccg atgaacgcg ttcatggtg tgggccaag 
      gacgagcgc cgcaaaatc ctcaaagcc tgccctgac 
      atgcacaac tcgaacata tcgaagatc ctcggcgcc 
      cgctggaag tccatgtcg aacgccgag aagcagccc 
      tactacgag gagcagtcc cgcctgtcg aagctgcac 
      atggagaag caccccgac taccgctac cgcccccgg 
      cccaagcgc acgtgcatc gtggacggc aagaagatg 
      cgcatctcg gagtacaag atgctgatg cggcagcgg 
      cgccaggag atgcggcag ctgtggtgc cgcgacggc 
      gcctccgac atgaacttc tcgctgtcg ggggcggac 
      ctgggggcg gcgacggcc acgcgggcc ggctccccc 
      atgagcatg gtgaacggc gcggggccc agcgccgag 
      gggttctac taccccgag ggggcctcg cccgacgcg 
      ctcaacttc gcacaggag cacgcgatg atgcacgac 
      tccagtccg aggaacgag gacgattga
    mRNA sequence
    • >TC007419 RA
      atgatcgac ctcctgctg gattcacaa caccacccg 
      ttcttgttc ttagctggt cgacctcaa ggcacccaa 
      caattagtc aatgaaacc cacaaacaa ttggagttc 
      caaagacgg cagcaggaa caaatgctg cagcagcaa 
      caaaaattg caagaactc caaggccag attacggca 
      cagtacgcc agtggcgtg gggcctcaa ggacttatg 
      ttcctcccg tttttggag caactgcga ggactccag 
      ccccccaaa tcccttgcc aatcacatg ctgcccccg 
      caccaagtc cccagctgg atctccgcg accgcccat 
      ctggcccac atgtcggcc agttcggac aagaggtcg 
      cccaccccc caacggacc ccgtcgccc ccagcgccg 
      ccgacggac cccgacgcc ccgctcaac ctctcgaaa 
      ccgaaatcg tcgagttcc ggttcggcg ggatcgagt 
      ccgcaaagt gccccggcc ggcttgcag actttcaac 
      gatcagccg gtggcggcc accgccccc aaacttcta 
      cctcccagt ttagtaatg ccgcgggcg ttcttgccc 
      tacgccgga ctgccccca cagtttagt ccactgccg 
      gcgagcgtc gaccggaag gggaaagat gagaagcaa 
      cctcatttc ccgatgcat atgtacggc ctgccggcg 
      cccccgcac atggggggc aggcccaag gacgacggc 
      ggcatcaag gatgagacc gacttcatg gcggcgtgt 
      cacatgtgg ggcggcccc gacccgaac ttcaagctg 
      gacgaaaac tccgaaaag gcgaaaatc atccgccag 
      caaccgaaa cgcgactcc gagaacaaa ccccacatc 
      aagcgcccg atgaacgcg ttcatggtg tgggccaag 
      gacgagcgc cgcaaaatc ctcaaagcc tgccctgac 
      atgcacaac tcgaacata tcgaagatc ctcggcgcc 
      cgctggaag tccatgtcg aacgccgag aagcagccc 
      tactacgag gagcagtcc cgcctgtcg aagctgcac 
      atggagaag caccccgac taccgctac cgcccccgg 
      cccaagcgc acgtgcatc gtggacggc aagaagatg 
      cgcatctcg gagtacaag atgctgatg cggcagcgg 
      cgccaggag atgcggcag ctgtggtgc cgcgacggc 
      gcctccgac atgaacttc tcgctgtcg ggggcggac 
      ctgggggcg gcgacggcc acgcgggcc ggctccccc 
      atgagcatg gtgaacggc gcggggccc agcgccgag 
      gggttctac taccccgag ggggcctcg cccgacgcg 
      ctcaacttc gcacaggag cacgcgatg atgcacgac 
      tccagtccg aggaacgag gacgattga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY