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Gene information
General information
TC007395 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5073529..5075051
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007395
    iBeetle screen iB_04321

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007395 RA
      MELNYKIQT YAWGKKGRG SKVANLHSN VNKNFKIDD 
      NSPYAELWL GTHVNGPSS LKNGEPLAN LIAKRPDYL 
      GANVRKVFG DQLPFLFKV LSVNQALSV QAHPSKKHA 
      EELHKQHPD IYKDPNHKP EMAIALTPF EALCGFRPL 
      PEIAFFLKN IPELKTATG DVADKHKHD SDLLKDAFK 
      TIFLTEKNT ISEQLKKLI HRLKTLDEK EREKLQASL 
      IERLYAQYP GDVGCFMVY FLNYLKLKP GEAIFLGPN 
      LPHAYIQGD CIECMACSD NVVRAGLTP KLIDFKTLC 
      DMVIYEGEK PEKKLFKPE IGDSCTLIF KPPVPDFAV 
      IQIKVPANM KNHVIKKTT SASILIVIE GKARSNDTD 
      LVPGSALFL PADQELKIE NITEDLLIY QALANV
    CDS sequence
    • >TC007395 RA
      atggaatta aattataaa atacaaact tacgcgtgg 
      ggtaaaaag ggcaggggg agtaaagtg gcgaatttg 
      cactcaaat gtgaacaaa aatttcaaa attgatgac 
      aattcaccg tacgccgaa ctttggtta gggacccat 
      gttaatggc ccttcgagt ttaaaaaac ggggaaccc 
      ctggcgaat ttaatagca aaacgtccg gattacttg 
      ggcgctaac gtccgcaag gtattcggt gatcagttg 
      ccgtttttg tttaaagtt ttgtcggtg aatcaggcc 
      ttgtccgtg caggcacat ccgtctaag aaacatgcc 
      gaagagtta cacaaacaa catcctgac atatacaag 
      gaccccaat cacaagcct gaaatggcc atcgcgttg 
      acacctttt gaagcactg tgcgggttt aggccattg 
      ccagaaatt gcatttttt cttaaaaac atcccggaa 
      ttgaaaact gcaacaggc gatgttgca gacaaacat 
      aaacacgac agcgatttg ttaaaagat gcatttaaa 
      actattttt ctaacagaa aaaaataca atcagcgag 
      caactaaaa aaattaata caccggctt aagactttg 
      gatgaaaaa gaaagggaa aaattgcaa gcaagtctg 
      attgagcgg ctttatgcg caataccct ggcgatgtg 
      ggttgtttc atggtgtac ttcctcaac tacttgaag 
      ctaaaacct ggcgaagct atatttttg gggcctaat 
      ttacctcac gcttacatt caaggagat tgcattgaa 
      tgcatggcg tgttcggac aacgtagtc cgtgctgga 
      cttaccccg aaattaatt gattttaaa actttgtgt 
      gatatggtt atttacgag ggtgagaaa ccagaaaag 
      aaactcttc aaaccagaa attggagac tcctgtact 
      ctaattttt aagcccccg gtacccgat ttcgcagtg 
      attcaaata aaggtccct gccaatatg aaaaatcat 
      gtaatcaaa aaaacgact tccgccagt attttgatt 
      gtgattgag ggcaaagcc aggtcaaat gataccgat 
      ttagtgcct ggaagcgcc ttgttcttg cccgcggat 
      caagaacta aaaattgag aacattacc gaagacttg 
      ctgatttat caagcactt gcaaatgtg taa
    mRNA sequence
    • >TC007395 RA
      aatgtcagt aacgtcaaa atttcataa ccttacagt 
      attcacaac taaataatg gaattaaat tataaaata 
      caaacttac gcgtggggt aaaaagggc agggggagt 
      aaagtggcg aatttgcac tcaaatgtg aacaaaaat 
      ttcaaaatt gatgacaat tcaccgtac gccgaactt 
      tggttaggg acccatgtt aatggccct tcgagttta 
      aaaaacggg gaacccctg gcgaattta atagcaaaa 
      cgtccggat tacttgggc gctaacgtc cgcaaggta 
      ttcggtgat cagttgccg tttttgttt aaagttttg 
      tcggtgaat caggccttg tccgtgcag gcacatccg 
      tctaagaaa catgccgaa gagttacac aaacaacat 
      cctgacata tacaaggac cccaatcac aagcctgaa 
      atggccatc gcgttgaca ccttttgaa gcactgtgc 
      gggtttagg ccattgcca gaaattgca ttttttctt 
      aaaaacatc ccggaattg aaaactgca acaggcgat 
      gttgcagac aaacataaa cacgacagc gatttgtta 
      aaagatgca tttaaaact atttttcta acagaaaaa 
      aatacaatc agcgagcaa ctaaaaaaa ttaatacac 
      cggcttaag actttggat gaaaaagaa agggaaaaa 
      ttgcaagca agtctgatt gagcggctt tatgcgcaa 
      taccctggc gatgtgggt tgtttcatg gtgtacttc 
      ctcaactac ttgaagcta aaacctggc gaagctata 
      tttttgggg cctaattta cctcacgct tacattcaa 
      ggagattgc attgaatgc atggcgtgt tcggacaac 
      gtagtccgt gctggactt accccgaaa ttaattgat 
      tttaaaact ttgtgtgat atggttatt tacgagggt 
      gagaaacca gaaaagaaa ctcttcaaa ccagaaatt 
      ggagactcc tgtactcta atttttaag cccccggta 
      cccgatttc gcagtgatt caaataaag gtccctgcc 
      aatatgaaa aatcatgta atcaaaaaa acgacttcc 
      gccagtatt ttgattgtg attgagggc aaagccagg 
      tcaaatgat accgattta gtgcctgga agcgccttg 
      ttcttgccc gcggatcaa gaactaaaa attgagaac 
      attaccgaa gacttgctg atttatcaa gcacttgca 
      aatgtgtaa attttgtat ttttatatt ggtgtaggt 
      tttataaac gggaataaa gcaatattt ttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY