Status
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Gene information
General information
TC007393 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5080644..5084411
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007393
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04320

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007393 RA
      MARNFVKEI KELNKTYVH IKDPEKLNA KLNGIITDG 
FQQLQVVSD FDQTLTKQH ENGKRHRSS FRIFECCPS 
VTEQYLEET NALTEKYFP MEYDPNISE EEKRKLMEE 
WWRQSEKIL CGLAVSCKE IDEAVAKVG SSLRDGTKD 
LFQALKNQD VPILVFSAG LGNIVVSVL KNQNVYLPN 
VKVISNFLK YNESGTIEG FTGPLIHVL NKNEFAIKN 
TEYYDLVKN RNNVILLGD SLGDAKMAD GMAHANHVL 
KIGFIYEKV EGNLPAYMD TFDIVLEDD QTMDVIRAI 
INLIK
    CDS sequence
    • >TC007393 RA
      atggcaaga aatttcgta aaagaaata aaagagtta 
aataaaacg tacgtccat attaaagac ccggaaaag 
ctgaacgct aaactaaac ggtataatt actgacggc 
ttccaacag ctgcaagtg gtgtcagac tttgaccaa 
acactcacc aaacagcac gagaatggc aagcgacac 
cggagcagt tttcgcatt tttgagtgc tgtccttcc 
gtcaccgag caatatttg gaagagaca aatgcgtta 
actgagaag tacttccca atggagtac gaccccaat 
atttcggaa gaggagaaa cgcaaattg atggaagag 
tggtggcga cagtcggaa aaaatccta tgtggttta 
gcagtcagt tgtaaggaa atcgacgaa gccgtggcc 
aaagtgggc agttcatta cgcgatggc actaaggac 
ctgttccag gccctcaaa aatcaggac gtgcctatt 
ttggttttc tcagccggt ctcggaaac atcgtggtt 
tccgtcttg aagaaccaa aatgtgtac ttaccaaac 
gttaaagtg atttcaaat tttttgaaa tacaacgaa 
agcggaacg attgaaggc ttcacaggc ccgctgata 
catgtttta aacaagaac gaattcgcg attaaaaat 
actgaatat tacgattta gtcaaaaat cgcaacaat 
gttattttg ttaggggac tcccttggt gacgctaaa 
atggcagat ggcatggct catgctaac cacgtactc 
aaaataggc ttcatctat gagaaggtt gagggcaat 
ttgcccgca tatatggac actttcgat atcgtgttg 
gaagacgac cagaccatg gatgtcata agagccata 
attaattta atcaaatga
    mRNA sequence
    • >TC007393 RA
      ctcggcgca aacagacta atagtgatc tgataacga 
caaaaagca gtcttaggt tatcatttc ttcaattac 
cgggttaaa aagcggcgc ctatctcaa ccctattca 
agcgcggac gacttatcg cgatctcgc attcagaaa 
actccgcat aagagactt gcgaaaaaa taaaatctg 
taatatatt tttaagccg ttccgcaat ggaattgat 
taacttatc acaaagagc tgacgcgca ctttacgac 
ttgaaccca gtctacagc aaggccact ttcgaccac 
ttgtagtta gatgctgct acatttcaa caaagttag 
cccacttct tcaaaacct acagaatta aagttagaa 
accatggca agaaatttc gtaaaagaa ataaaagag 
ttaaataaa acgtacgtc catattaaa gacccggaa 
aagctgaac gctaaacta aacggtata attactgac 
ggcttccaa cagctgcaa gtggtgtca gactttgac 
caaacactc accaaacag cacgagaat ggcaagcga 
caccggagc agttttcgc atttttgag tgctgtcct 
tccgtcacc gagcaatat ttggaagag acaaatgcg 
ttaactgag aagtacttc ccaatggag tacgacccc 
aatatttcg gaagaggag aaacgcaaa ttgatggaa 
gagtggtgg cgacagtcg gaaaaaatc ctatgtggt 
ttagcagtc agttgtaag gaaatcgac gaagccgtg 
gccaaagtg ggcagttca ttacgcgat ggcactaag 
gacctgttc caggccctc aaaaatcag gacgtgcct 
attttggtt ttctcagcc ggtctcgga aacatcgtg 
gtttccgtc ttgaagaac caaaatgtg tacttacca 
aacgttaaa gtgatttca aattttttg aaatacaac 
gaaagcgga acgattgaa ggcttcaca ggcccgctg 
atacatgtt ttaaacaag aacgaattc gcgattaaa 
aatactgaa tattacgat ttagtcaaa aatcgcaac 
aatgttatt ttgttaggg gactccctt ggtgacgct 
aaaatggca gatggcatg gctcatgct aaccacgta 
ctcaaaata ggcttcatc tatgagaag gttgagggc 
aatttgccc gcatatatg gacactttc gatatcgtg 
ttggaagac gaccagacc atggatgtc ataagagcc 
ataattaat ttaatcaaa tgattggtg cttacatat 
cactgttta cttaactga aatatttta atattcggg 
tcagatcat ctctgatct atttttagt caaattagt 
tcccaagaa acttattta tgcttaagt cattgtttt 
tctgaaaaa caatggaga ataggagtg ctctcaatt 
tgtatattt tttcacaaa tttaatttg ctcactaat 
tgtttactt aattataca atcctgatt tgtaaggtc 
aagaaattt gctattatc aatctcgtg atgatgtgt 
cacttttta tgttttatt gaacaaata aacaggaaa 
atgacag

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY