Status
Please wait ...
Gene information
General information
TC007376 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5364751..5370122
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007376
    iBeetle screen iB_04318

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007376 RA
      MSRSGDILG IIRGLQLVV EAAAKLHSS ELKQIWANS 
      NYRVFIQDC ITQNRTNSN NVTNIVSET ADRVATVCH 
      GLKAYASNT SPKDGYYQN LNDASEDLK EPPRAESDR 
      AQFQIKLTK SDQELLKKL DLEHQEKLR QVEMEQDKA 
      KKRDEEKKA ESKGQVTAS PNPKSKLKL SEHSKQRKV 
      PSSRIGRMI SFGGLAAGL GFGTATEYA KRTFGFGQS 
      ADPSTLFLN PNNLDRIVD TLCKVRGAA LKLGQLLSI 
      QDDSVINPE LAKALERVR KSADFMPNW QVEQVMTAE 
      LGPNWRSQF LEFEEKPFA AASIGQVHW GRISDGREV 
      AIKIQYPGV AKGIESDID NLGGIMKFW DVFPRGMFL 
      ENLMRVAKR ELAWEVDYR REAECTRIF RRLLAPYSD 
      YYVPEVIDE LSTNQVFTT ELLDGVPVD QCFDMPPED 
      RRFIAEKII ELCLLELLE FRYMQTDPN WANFLYNPR 
      KKQIMLLDF GASREYSKP FMDQYVKIL KASCDADRE 
      TVLQVSREL GFLTGYESK VMEDAHVDA VMILGEVFR 
      CADAYDFAA QDMTARIQN LVPTMVTHR LCPPPEEVY 
      SLHRKLSGV FLLCSKLKT SVSCRDKFL SLYSKYTSA 
      V
    CDS sequence
    • >TC007376 RA
      atgtctcgt tctggcgat attttggga ataattcgc 
      ggtttacag cttgtggtt gaggcagcc gcaaaattg 
      cactcttcc gaactcaaa cagatctgg gcaaactca 
      aattatcgc gtctttatc caagactgc atcacccaa 
      aatcgaact aactcaaac aatgtcact aacatcgtt 
      tcggagact gcggaccgc gttgccaca gtctgccat 
      ggccttaag gcgtacgcc tctaacaca tctcctaaa 
      gatggttat tatcaaaac ttaaacgat gccagtgag 
      gatttgaaa gagccgcca agggccgaa agtgacagg 
      gcgcaattt caaattaaa ttaactaaa agtgaccaa 
      gaactgctg aagaagcta gatttggaa catcaggag 
      aaattgaga caggtggag atggaacaa gacaaggcg 
      aaaaagcgg gatgaagag aaaaaggcg gagagcaag 
      ggccaagtc acggccagt ccgaacccc aagtcaaaa 
      ttgaagttg agcgaacac tcaaaacag cgaaaagtc 
      ccgtcatct agaatcggc cgaatgata tcttttgga 
      ggccttgcg gcgggctta ggtttcggg acggcaaca 
      gaatatgcg aaaagaact tttggtttt ggccagtcg 
      gcagaccct tccaccctt ttcctcaac cccaacaac 
      ttggaccga atcgtggat acgttgtgt aaagtcaga 
      ggagcggcc ttaaagctg ggccagttg ctgagcatc 
      caggacgac agtgtaatc aatcccgag ttggctaag 
      gccctagaa agggtgcga aaatcggcc gattttatg 
      ccgaattgg caagttgag caagtcatg actgctgaa 
      ttggggcca aattggcgt tcccagttt ttggagttt 
      gaggaaaaa ccgttcgct gctgcgtca attggccaa 
      gtccattgg gggcgaatt agcgacgga cgtgaagtt 
      gctatcaaa atccaatat ccgggagtt gcgaaaggc 
      attgagagt gatattgat aatctggga gggattatg 
      aaattttgg gacgttttt ccccgggga atgttcttg 
      gagaattta atgcgagtt gcgaaaaga gagttggct 
      tgggaggtg gattacagg agagaggcc gagtgtacg 
      aggattttt cgacgattg ttagctcct tatagtgat 
      tactacgta cctgaagtt atagatgaa ctatcaacg 
      aatcaagta tttacaacc gagcttctt gacggtgtt 
      ccggtcgac caatgcttc gatatgccg cccgaagac 
      cggcgtttc atcgctgaa aaaatcata gagttgtgt 
      ttactcgag ttgcttgag ttcagatac atgcagacc 
      gatccaaac tgggccaat ttcctatac aacccacga 
      aaaaaacaa atcatgttg ctcgatttc ggagccagt 
      cgtgaatat tccaagcct tttatggat cagtacgtt 
      aaaattctt aaagcgtcg tgtgacgcc gatcgagaa 
      acggtcttg caagtctcg cgggaattg ggatttttg 
      acgggttat gagagcaag gtgatggaa gatgctcac 
      gtagacgcc gtgatgata ttaggtgaa gtcttccga 
      tgtgcggac gcttacgat ttcgctgct caagatatg 
      acggccaga atacaaaat ttggttccg acaatggtc 
      acgcaccgg ctttgtcca cctccggaa gaagtgtat 
      tccttacat aggaaatta tcaggggtt ttcttgctg 
      tgttcgaaa ctaaaaacc tccgtttct tgcagggat 
      aaattctta agtttgtat agcaaatat accagtgcc 
      gtgtag
    mRNA sequence
    • >TC007376 RA
      aacctcaca ttagcgtgg gtgtttttc attgattgt 
      aaattaatt ttgcgtttt tctgagtaa attagtacc 
      taatgtctc gttctggcg atattttgg gaataattc 
      gcggtttac agcttgtgg ttgaggcag ccgcaaaat 
      tgcactctt ccgaactca aacagatct gggcaaact 
      caaattatc gcgtcttta tccaagact gcatcaccc 
      aaaatcgaa ctaactcaa acaatgtca ctaacatcg 
      tttcggaga ctgcggacc gcgttgcca cagtctgcc 
      atggcctta aggcgtacg cctctaaca catctccta 
      aagatggtt attatcaaa acttaaacg atgccagtg 
      aggatttga aagagccgc caagggccg aaagtgaca 
      gggcgcaat ttcaaatta aattaacta aaagtgacc 
      aagaactgc tgaagaagc tagatttgg aacatcagg 
      agaaattga gacaggtgg agatggaac aagacaagg 
      cgaaaaagc gggatgaag agaaaaagg cggagagca 
      agggccaag tcacggcca gtccgaacc ccaagtcaa 
      aattgaagt tgagcgaac actcaaaac agcgaaaag 
      tcccgtcat ctagaatcg gccgaatga tatcttttg 
      gaggccttg cggcgggct taggtttcg ggacggcaa 
      cagaatatg cgaaaagaa cttttggtt ttggccagt 
      cggcagacc cttccaccc ttttcctca accccaaca 
      acttggacc gaatcgtgg atacgttgt gtaaagtca 
      gaggagcgg ccttaaagc tgggccagt tgctgagca 
      tccaggacg acagtgtaa tcaatcccg agttggcta 
      aggccctag aaagggtgc gaaaatcgg ccgatttta 
      tgccgaatt ggcaagttg agcaagtca tgactgctg 
      aattggggc caaattggc gttcccagt ttttggagt 
      ttgaggaaa aaccgttcg ctgctgcgt caattggcc 
      aagtccatt gggggcgaa ttagcgacg gacgtgaag 
      ttgctatca aaatccaat atccgggag ttgcgaaag 
      gcattgaga gtgatattg ataatctgg gagggatta 
      tgaaatttt gggacgttt ttccccggg gaatgttct 
      tggagaatt taatgcgag ttgcgaaaa gagagttgg 
      cttgggagg tggattaca ggagagagg ccgagtgta 
      cgaggattt ttcgacgat tgttagctc cttatagtg 
      attactacg tacctgaag ttatagatg aactatcaa 
      cgaatcaag tatttacaa ccgagcttc ttgacggtg 
      ttccggtcg accaatgct tcgatatgc cgcccgaag 
      accggcgtt tcatcgctg aaaaaatca tagagttgt 
      gtttactcg agttgcttg agttcagat acatgcaga 
      ccgatccaa actgggcca atttcctat acaacccac 
      gaaaaaaac aaatcatgt tgctcgatt tcggagcca 
      gtcgtgaat attccaagc cttttatgg atcagtacg 
      ttaaaattc ttaaagcgt cgtgtgacg ccgatcgag 
      aaacggtct tgcaagtct cgcgggaat tgggatttt 
      tgacgggtt atgagagca aggtgatgg aagatgctc 
      acgtagacg ccgtgatga tattaggtg aagtcttcc 
      gatgtgcgg acgcttacg atttcgctg ctcaagata 
      tgacggcca gaatacaaa atttggttc cgacaatgg 
      tcacgcacc ggctttgtc cacctccgg aagaagtgt 
      attccttac ataggaaat tatcagggg ttttcttgc 
      tgtgttcga aactaaaaa cctccgttt cttgcaggg 
      ataaattct taagtttgt atagcaaat ataccagtg 
      ccgtgtagt tttgtagcg ttggaatgt aacaggttt 
      tgtaacaaa ttttaaata aagattaat caaattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY