Status
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Gene information
General information
TC007376 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5364751..5370122
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007376
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04318

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007376 RA
      MSRSGDILG IIRGLQLVV EAAAKLHSS ELKQIWANS 
NYRVFIQDC ITQNRTNSN NVTNIVSET ADRVATVCH 
GLKAYASNT SPKDGYYQN LNDASEDLK EPPRAESDR 
AQFQIKLTK SDQELLKKL DLEHQEKLR QVEMEQDKA 
KKRDEEKKA ESKGQVTAS PNPKSKLKL SEHSKQRKV 
PSSRIGRMI SFGGLAAGL GFGTATEYA KRTFGFGQS 
ADPSTLFLN PNNLDRIVD TLCKVRGAA LKLGQLLSI 
QDDSVINPE LAKALERVR KSADFMPNW QVEQVMTAE 
LGPNWRSQF LEFEEKPFA AASIGQVHW GRISDGREV 
AIKIQYPGV AKGIESDID NLGGIMKFW DVFPRGMFL 
ENLMRVAKR ELAWEVDYR REAECTRIF RRLLAPYSD 
YYVPEVIDE LSTNQVFTT ELLDGVPVD QCFDMPPED 
RRFIAEKII ELCLLELLE FRYMQTDPN WANFLYNPR 
KKQIMLLDF GASREYSKP FMDQYVKIL KASCDADRE 
TVLQVSREL GFLTGYESK VMEDAHVDA VMILGEVFR 
CADAYDFAA QDMTARIQN LVPTMVTHR LCPPPEEVY 
SLHRKLSGV FLLCSKLKT SVSCRDKFL SLYSKYTSA 
V
    CDS sequence
    • >TC007376 RA
      atgtctcgt tctggcgat attttggga ataattcgc 
ggtttacag cttgtggtt gaggcagcc gcaaaattg 
cactcttcc gaactcaaa cagatctgg gcaaactca 
aattatcgc gtctttatc caagactgc atcacccaa 
aatcgaact aactcaaac aatgtcact aacatcgtt 
tcggagact gcggaccgc gttgccaca gtctgccat 
ggccttaag gcgtacgcc tctaacaca tctcctaaa 
gatggttat tatcaaaac ttaaacgat gccagtgag 
gatttgaaa gagccgcca agggccgaa agtgacagg 
gcgcaattt caaattaaa ttaactaaa agtgaccaa 
gaactgctg aagaagcta gatttggaa catcaggag 
aaattgaga caggtggag atggaacaa gacaaggcg 
aaaaagcgg gatgaagag aaaaaggcg gagagcaag 
ggccaagtc acggccagt ccgaacccc aagtcaaaa 
ttgaagttg agcgaacac tcaaaacag cgaaaagtc 
ccgtcatct agaatcggc cgaatgata tcttttgga 
ggccttgcg gcgggctta ggtttcggg acggcaaca 
gaatatgcg aaaagaact tttggtttt ggccagtcg 
gcagaccct tccaccctt ttcctcaac cccaacaac 
ttggaccga atcgtggat acgttgtgt aaagtcaga 
ggagcggcc ttaaagctg ggccagttg ctgagcatc 
caggacgac agtgtaatc aatcccgag ttggctaag 
gccctagaa agggtgcga aaatcggcc gattttatg 
ccgaattgg caagttgag caagtcatg actgctgaa 
ttggggcca aattggcgt tcccagttt ttggagttt 
gaggaaaaa ccgttcgct gctgcgtca attggccaa 
gtccattgg gggcgaatt agcgacgga cgtgaagtt 
gctatcaaa atccaatat ccgggagtt gcgaaaggc 
attgagagt gatattgat aatctggga gggattatg 
aaattttgg gacgttttt ccccgggga atgttcttg 
gagaattta atgcgagtt gcgaaaaga gagttggct 
tgggaggtg gattacagg agagaggcc gagtgtacg 
aggattttt cgacgattg ttagctcct tatagtgat 
tactacgta cctgaagtt atagatgaa ctatcaacg 
aatcaagta tttacaacc gagcttctt gacggtgtt 
ccggtcgac caatgcttc gatatgccg cccgaagac 
cggcgtttc atcgctgaa aaaatcata gagttgtgt 
ttactcgag ttgcttgag ttcagatac atgcagacc 
gatccaaac tgggccaat ttcctatac aacccacga 
aaaaaacaa atcatgttg ctcgatttc ggagccagt 
cgtgaatat tccaagcct tttatggat cagtacgtt 
aaaattctt aaagcgtcg tgtgacgcc gatcgagaa 
acggtcttg caagtctcg cgggaattg ggatttttg 
acgggttat gagagcaag gtgatggaa gatgctcac 
gtagacgcc gtgatgata ttaggtgaa gtcttccga 
tgtgcggac gcttacgat ttcgctgct caagatatg 
acggccaga atacaaaat ttggttccg acaatggtc 
acgcaccgg ctttgtcca cctccggaa gaagtgtat 
tccttacat aggaaatta tcaggggtt ttcttgctg 
tgttcgaaa ctaaaaacc tccgtttct tgcagggat 
aaattctta agtttgtat agcaaatat accagtgcc 
gtgtag
    mRNA sequence
    • >TC007376 RA
      aacctcaca ttagcgtgg gtgtttttc attgattgt 
aaattaatt ttgcgtttt tctgagtaa attagtacc 
taatgtctc gttctggcg atattttgg gaataattc 
gcggtttac agcttgtgg ttgaggcag ccgcaaaat 
tgcactctt ccgaactca aacagatct gggcaaact 
caaattatc gcgtcttta tccaagact gcatcaccc 
aaaatcgaa ctaactcaa acaatgtca ctaacatcg 
tttcggaga ctgcggacc gcgttgcca cagtctgcc 
atggcctta aggcgtacg cctctaaca catctccta 
aagatggtt attatcaaa acttaaacg atgccagtg 
aggatttga aagagccgc caagggccg aaagtgaca 
gggcgcaat ttcaaatta aattaacta aaagtgacc 
aagaactgc tgaagaagc tagatttgg aacatcagg 
agaaattga gacaggtgg agatggaac aagacaagg 
cgaaaaagc gggatgaag agaaaaagg cggagagca 
agggccaag tcacggcca gtccgaacc ccaagtcaa 
aattgaagt tgagcgaac actcaaaac agcgaaaag 
tcccgtcat ctagaatcg gccgaatga tatcttttg 
gaggccttg cggcgggct taggtttcg ggacggcaa 
cagaatatg cgaaaagaa cttttggtt ttggccagt 
cggcagacc cttccaccc ttttcctca accccaaca 
acttggacc gaatcgtgg atacgttgt gtaaagtca 
gaggagcgg ccttaaagc tgggccagt tgctgagca 
tccaggacg acagtgtaa tcaatcccg agttggcta 
aggccctag aaagggtgc gaaaatcgg ccgatttta 
tgccgaatt ggcaagttg agcaagtca tgactgctg 
aattggggc caaattggc gttcccagt ttttggagt 
ttgaggaaa aaccgttcg ctgctgcgt caattggcc 
aagtccatt gggggcgaa ttagcgacg gacgtgaag 
ttgctatca aaatccaat atccgggag ttgcgaaag 
gcattgaga gtgatattg ataatctgg gagggatta 
tgaaatttt gggacgttt ttccccggg gaatgttct 
tggagaatt taatgcgag ttgcgaaaa gagagttgg 
cttgggagg tggattaca ggagagagg ccgagtgta 
cgaggattt ttcgacgat tgttagctc cttatagtg 
attactacg tacctgaag ttatagatg aactatcaa 
cgaatcaag tatttacaa ccgagcttc ttgacggtg 
ttccggtcg accaatgct tcgatatgc cgcccgaag 
accggcgtt tcatcgctg aaaaaatca tagagttgt 
gtttactcg agttgcttg agttcagat acatgcaga 
ccgatccaa actgggcca atttcctat acaacccac 
gaaaaaaac aaatcatgt tgctcgatt tcggagcca 
gtcgtgaat attccaagc cttttatgg atcagtacg 
ttaaaattc ttaaagcgt cgtgtgacg ccgatcgag 
aaacggtct tgcaagtct cgcgggaat tgggatttt 
tgacgggtt atgagagca aggtgatgg aagatgctc 
acgtagacg ccgtgatga tattaggtg aagtcttcc 
gatgtgcgg acgcttacg atttcgctg ctcaagata 
tgacggcca gaatacaaa atttggttc cgacaatgg 
tcacgcacc ggctttgtc cacctccgg aagaagtgt 
attccttac ataggaaat tatcagggg ttttcttgc 
tgtgttcga aactaaaaa cctccgttt cttgcaggg 
ataaattct taagtttgt atagcaaat ataccagtg 
ccgtgtagt tttgtagcg ttggaatgt aacaggttt 
tgtaacaaa ttttaaata aagattaat caaattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY