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Gene information
General information
TC007333 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6036870..6053976
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007333
    iBeetle screen iB_04315

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    gnathal appendages sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    labium partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen posterior not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007333 RA
      MWFYLCFFF LFQSYNCEQ ESAKNETFD DLFDHKTNK 
      SSENNTIKL DRITQPSAQ TQHIERAIN SPNSTKPQS 
      EFRPSPHLE TYYEFNKFP VPPALPEAK HFSHLNFGE 
      SLPHWPTES PWSSKIKFP TTSPVPTTK ERPYEFYNA 
      EKSTGKSYN VPTKTDQLV HKNHFDFSA PVPPSGGSK 
      LGAIATPEH DALGSPWKK IIKFLTAFI PIGLLISAL 
      TPTVITVQS MNGTQLRSR AEKSDQPAN KLLSSLNYF 
      NSVGCEDRI LCELVLSAS TTQNAEQHI ENLLNTFTE 
      DGKAVEKAE EMRRIFEAV KRQDCRPII CKNMKDKTR 
      QPVQLNAGN PREHAISVL VSLVIYTDY TMGLLKVLL 
      LLATCSAIR TEPEKPPPT ITRTEIKVS RSLNPEDMQ 
      TLNVMTKDG NVAQLIVKR RDRKTEQQD DRKNQFSRA 
      IYANWIPVS SFYYQPNII RLDTIALVR NATQEKKPN 
      VVDSDRIPN VPKPVTIRS GDIFIKNNE PKRARSIVQ 
      MDQDGIPVI HGVRVPDDE TDRQTWRNA RVINGELVP 
      YEEGYKPPP AVPIGQLIY ASQVKDKED SRSIGPFTK 
      QDNYKSEDN HNSFGPFTV KDNLPAEKP KQNEDYVRF 
      NSQSGIGPF TKADNAKIT SSKLIDYIK EINAKESKR 
      DYFARRKYR SYDDNTQMQ RRMLQYPGQ PSYPNSLLY 
      TPTSKLSPV TFNEGVRTP VLQYAHPEL GVQPAKATP 
      EDEEEMVYK DNQYEVDSG RQSQYDGVN SVNYYRKDV 
      INYPYNTYY YKPKPEQPF WIRISESIK DNVQNGFAR 
      MQQLTRPVF EPLVEATHK ISHNLGFSK EPQAQEKVG 
      LIAPMGSSV ILPALGLVA GGAALGLGA AAVGRFLNP 
      NDMRAFGEV NPNDIVVIM EEPPQDGHE EHKRFRRNA 
      EDEYYMQQL VANVEKDKG LHHLAAPHF WSDTSCAKR 
      LFCEVMVRQ NDDEVVLME KKMDSLMAS VHPDVANQV 
      SHHLQQVMD SVKMRDCSK LYCNRRYPV PAPAA
    CDS sequence
    • >TC007333 RA
      atgtggttt tacttatgc ttttttttt ctcttccaa 
      agttacaat tgtgagcaa gaaagtgca aaaaatgag 
      acttttgac gatttattt gaccacaaa acgaacaaa 
      tcaagcgag aacaataca atcaaatta gatagaata 
      acgcaaccc tcggcccaa acccaacac atcgaacgc 
      gccatcaat tcaccaaac tcgactaag ccccagtcc 
      gaattcagg cctagccca cacctggaa acctactac 
      gaatttaac aaatttcca gtgcccccg gctttgccc 
      gaagccaag catttttca catttgaat tttggcgag 
      tctttacca cactggcct acggagtct ccctggtcg 
      tcaaaaatt aaattccca acgacttca ccggtacca 
      accaccaaa gaacgccct tatgagttt tacaacgcg 
      gagaaaagc accggaaaa tcgtacaac gtaccgaca 
      aaaaccgac caacttgtg cacaaaaac cacttcgat 
      tttagcgcc ccggtacca cccagtggc gggtccaag 
      ctgggcgct atcgcgaca cccgagcat gacgctctg 
      ggaagcccc tggaagaaa ataatcaaa tttttgacg 
      gctttcatc cccattggg ttgctgata agtgcgcta 
      acgccgacc gtgataacg gtccagtca atgaacggg 
      acacagttg aggtcacga gcggaaaag tcggaccag 
      ccagcgaac aaattgctt tcttccctc aattatttc 
      aattcggtt gggtgcgaa gaccggatt ttgtgtgag 
      ttggtactg agcgcaagt accactcaa aacgccgag 
      caacacatt gagaacctg ctcaacact ttcactgaa 
      gatggaaaa gcggtcgag aaagccgaa gagatgaga 
      aggatcttc gaggcggtt aaacggcaa gactgccgg 
      cctattatt tgcaaaaat atgaaagac aagacacga 
      caaccggtg cagttgaat gctggaaac cctcgtgaa 
      cacgcgatt tcagtgctt gtgtcgttg gtgatttat 
      acggattat accatgggg ctgctcaag gtgctccta 
      cttttggcc acgtgttcc gccatccga acggaaccg 
      gaaaagccg ccgccgaca atcacccgc accgaaatc 
      aaagtctcc aggagcttg aaccccgaa gacatgcaa 
      acgctcaac gtcatgacc aaagatggg aatgtggcc 
      cagcttata gtcaaacga cgcgaccgc aaaactgag 
      caacaagat gacaggaaa aaccaattt tccagggct 
      atttacgcc aattggata ccggtctcc tccttctac 
      taccagccc aacatcata cgcttggac actattgcc 
      ttggtccgg aacgccacc caggagaag aagcccaac 
      gttgtggac agcgacaga attcctaac gtgcctaag 
      cccgttacc atacgctct ggagacatt ttcatcaaa 
      aacaacgag ccaaagcgg gcgcgttcc atcgtccag 
      atggaccaa gacggcatt ccggtgatt cacggagtg 
      cgagttcct gatgacgaa accgaccgt caaacttgg 
      cgaaacgct cgagtcatc aatggagaa ctggtacca 
      tatgaggaa ggttacaag ccaccaccg gctgtcccc 
      atcggtcag ctaatctac gccagtcaa gtcaaagac 
      aaagaagat tccagaagc attggtcct ttcaccaaa 
      caggacaac tacaagagc gaggataat cacaattcg 
      ttcggtccc ttcactgtt aaggacaat ctcccggcg 
      gaaaagccc aaacaaaac gaagattat gtcaggttt 
      aacagccag tcgggaatt ggcccgttt acgaaagcc 
      gacaacgcc aaaatcaca agctcgaag ttgatcgat 
      tacatcaag gaaatcaac gcgaaggaa agcaaacgc 
      gattatttc gcaagacgg aagtaccgc tcgtacgat 
      gacaacaca cagatgcag agaaggatg ttgcaatat 
      ccgggccag ccgtcctat cccaactcc ttgctctac 
      acacctacg tccaagttg agcccagtc acgttcaat 
      gagggcgtt agaactccg gttttgcaa tacgcgcac 
      ccggaactg ggcgtccaa cccgccaaa gctaccccg 
      gaggacgaa gaagaaatg gtttacaag gacaaccaa 
      tacgaagtt gacagtggc cgacaatca caatacgat 
      ggggtcaac agtgtcaat tactaccgg aaggacgtc 
      atcaattat ccttacaac acctactac tacaagccg 
      aagccggag caaccgttc tggatccgc atctctgaa 
      agcatcaaa gacaacgtc cagaacggt tttgcccga 
      atgcagcaa ctgacccgt ccagtgttc gagcctttg 
      gtcgaagcc acccacaaa atatcgcac aatttgggc 
      ttttccaag gagccgcaa gcccaggag aaggtgggg 
      ctgatagcc cccatgggc agttcggtg attttgccc 
      gcgctgggg ctcgtggcg ggcggggcg gccctgggg 
      cttggcgcg gctgcggtt ggtcgcttc ctaaacccc 
      aacgacatg cgggcgttc ggtgaagtt aacccgaac 
      gatattgtg gttattatg gaggagccg ccgcaggac 
      ggccacgag gagcataag aggttccgg cgaaacgcc 
      gaagatgag tattacatg cagcagttg gttgcaaat 
      gttgagaaa gacaaaggg ttgcatcat ctcgccgct 
      ccgcatttt tggtcggac acgtcctgt gccaagcgg 
      ctgttttgc gaggtgatg gtgcggcag aacgacgac 
      gaggtggtc ttgatggag aagaaaatg gacagtttg 
      atggcatcg gtgcatcca gacgtcgcc aatcaggtc 
      tcgcaccat ctccagcaa gtcatggat tcggtcaaa 
      atgagggac tgctccaag ctttactgc aacaggagg 
      taccctgtt ccggctccg gcggcctaa
    mRNA sequence
    • >TC007333 RA
      aaagtgcag tttcgtcac aatgtggtt ttacttatg 
      ctttttttt tctcttcca aagttacaa ttgtgagca 
      agaaagtgc aaaaaatga gacttttga cgatttatt 
      tgaccacaa aacgaacaa atcaagcga gaacaatac 
      aatcaaatt agatagaat aacgcaacc ctcggccca 
      aacccaaca catcgaacg cgccatcaa ttcaccaaa 
      ctcgactaa gccccagtc cgaattcag gcctagccc 
      acacctgga aacctacta cgaatttaa caaatttcc 
      agtgccccc ggctttgcc cgaagccaa gcatttttc 
      acatttgaa ttttggcga gtctttacc acactggcc 
      tacggagtc tccctggtc gtcaaaaat taaattccc 
      aacgacttc accggtacc aaccaccaa agaacgccc 
      ttatgagtt ttacaacgc ggagaaaag caccggaaa 
      atcgtacaa cgtaccgac aaaaaccga ccaacttgt 
      gcacaaaaa ccacttcga ttttagcgc cccggtacc 
      acccagtgg cgggtccaa gctgggcgc tatcgcgac 
      acccgagca tgacgctct gggaagccc ctggaagaa 
      aataatcaa atttttgac ggctttcat ccccattgg 
      gttgctgat aagtgcgct aacgccgac cgtgataac 
      ggtccagtc aatgaacgg gacacagtt gaggtcacg 
      agcggaaaa gtcggacca gccagcgaa caaattgct 
      ttcttccct caattattt caattcggt tgggtgcga 
      agaccggat tttgtgtga gttggtact gagcgcaag 
      taccactca aaacgccga gcaacacat tgagaacct 
      gctcaacac tttcactga agatggaaa agcggtcga 
      gaaagccga agagatgag aaggatctt cgaggcggt 
      taaacggca agactgccg gcctattat ttgcaaaaa 
      tatgaaaga caagacacg acaaccggt gcagttgaa 
      tgctggaaa ccctcgtga acacgcgat ttcagtgct 
      tgtgtcgtt ggtgattta tacggatta taccatggg 
      gctgctcaa ggtgctcct acttttggc cacgtgttc 
      cgccatccg aacggaacc ggaaaagcc gccgccgac 
      aatcacccg caccgaaat caaagtctc caggagctt 
      gaaccccga agacatgca aacgctcaa cgtcatgac 
      caaagatgg gaatgtggc ccagcttat agtcaaacg 
      acgcgaccg caaaactga gcaacaaga tgacaggaa 
      aaaccaatt ttccagggc tatttacgc caattggat 
      accggtctc ctccttcta ctaccagcc caacatcat 
      acgcttgga cactattgc cttggtccg gaacgccac 
      ccaggagaa gaagcccaa cgttgtgga cagcgacag 
      aattcctaa cgtgcctaa gcccgttac catacgctc 
      tggagacat tttcatcaa aaacaacga gccaaagcg 
      ggcgcgttc catcgtcca gatggacca agacggcat 
      tccggtgat tcacggagt gcgagttcc tgatgacga 
      aaccgaccg tcaaacttg gcgaaacgc tcgagtcat 
      caatggaga actggtacc atatgagga aggttacaa 
      gccaccacc ggctgtccc catcggtca gctaatcta 
      cgccagtca agtcaaaga caaagaaga ttccagaag 
      cattggtcc tttcaccaa acaggacaa ctacaagag 
      cgaggataa tcacaattc gttcggtcc cttcactgt 
      taaggacaa tctcccggc ggaaaagcc caaacaaaa 
      cgaagatta tgtcaggtt taacagcca gtcgggaat 
      tggcccgtt tacgaaagc cgacaacgc caaaatcac 
      aagctcgaa gttgatcga ttacatcaa ggaaatcaa 
      cgcgaagga aagcaaacg cgattattt cgcaagacg 
      gaagtaccg ctcgtacga tgacaacac acagatgca 
      gagaaggat gttgcaata tccgggcca gccgtccta 
      tcccaactc cttgctcta cacacctac gtccaagtt 
      gagcccagt cacgttcaa tgagggcgt tagaactcc 
      ggttttgca atacgcgca cccggaact gggcgtcca 
      acccgccaa agctacccc ggaggacga agaagaaat 
      ggtttacaa ggacaacca atacgaagt tgacagtgg 
      ccgacaatc acaatacga tggggtcaa cagtgtcaa 
      ttactaccg gaaggacgt catcaatta tccttacaa 
      cacctacta ctacaagcc gaagccgga gcaaccgtt 
      ctggatccg catctctga aagcatcaa agacaacgt 
      ccagaacgg ttttgcccg aatgcagca actgacccg 
      tccagtgtt cgagccttt ggtcgaagc cacccacaa 
      aatatcgca caatttggg cttttccaa ggagccgca 
      agcccagga gaaggtggg gctgatagc ccccatggg 
      cagttcggt gattttgcc cgcgctggg gctcgtggc 
      gggcggggc ggccctggg gcttggcgc ggctgcggt 
      tggtcgctt cctaaaccc caacgacat gcgggcgtt 
      cggtgaagt taacccgaa cgatattgt ggttattat 
      ggaggagcc gccgcagga cggccacga ggagcataa 
      gaggttccg gcgaaacgc cgaagatga gtattacat 
      gcagcagtt ggttgcaaa tgttgagaa agacaaagg 
      gttgcatca tctcgccgc tccgcattt ttggtcgga 
      cacgtcctg tgccaagcg gctgttttg cgaggtgat 
      ggtgcggca gaacgacga cgaggtggt cttgatgga 
      gaagaaaat ggacagttt gatggcatc ggtgcatcc 
      agacgtcgc caatcaggt ctcgcacca tctccagca 
      agtcatgga ttcggtcaa aatgaggga ctgctccaa 
      gctttactg caacaggag gtaccctgt tccggctcc 
      ggcggccta atctaatca cttatcaca agaattgtc 
      catgaccaa cgcatctga atatttatt ttttattgt 
      tagtagttt cgctcgaaa cattatatt aatgttacc 
      ttttatcga atatacgtt aattacaac caattacag 
      acgcctttg tctggcttt tgccatatt tgtgtacgt 
      atggtggta aattgttga gtacagcac gcgccaaaa 
      ttgtgaaat agtttgcag aattggcca ataaacgcg 
      caaactgtc cattattac ttagtaaat tgtcagtgc 
      tagataaag tgataaata attgtacat agtcacccc 
      cccttcttt taatttata aatgttacg ctaagagat 
      aatttctgt taactattc caataaagc tataaaccg 
      tac
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