Status
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Gene information
General information
TC007322 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6206191..6208396
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007322
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04314

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007322 RA
      MAIFTSSIF IDIITIITT VLALTIAFF KWKLTFWEK 
      LGVPTLNPV LFFGDAKNI LLGKRQVGV EFIDYYKQF 
      KPRGLKHGG VYLGPRPFW VITDPGLIK HILQIDFPH 
      FINHGTYAD EENDPLSAH LFSLEGTKW KNMRTKMTP 
      TFTSGKMKM MFQTLVDCT PGLKDIMDE HASKKKAVN 
      IKNILERFT TDIIGSVGF GIECNSLKD PDSLFPKYG 
      KKVFDLTPA EQLKQSLLF FLPNPVLKA MKFRITNKD 
      VEDFFMKTV RETVLYREK NNIYRKDFM HLLLQLKNR 
      GTVTDDEKI IDHSNQKQE PALTMNELT AQAFVFFLA 
      GFETSSTTM TFALYELAT NPDIQEKLR NEICTVLEK 
      HNNELTYNA IAEMTYMDQ VLHETLRKY PPLPILQRQ 
      CNKDYIVPE TNIRIPKGV DVAITILAL HRDPEFYPN 
      PEVFDPERF NEENKNART PFTWLPFGD GPRVCIGLR 
      FGMMQSKVG LTALLKDYR VTLNKKTKV PLEMSKNSF 
      IIAAEGGIW LDIEKINT
    CDS sequence
    • >TC007322 RA
      atggcaatt ttcacgtca agcattttc atcgacata 
      ataaccata atcacaaca gtgctcgcc ttaaccatc 
      gcatttttc aaatggaaa ttgacattt tgggaaaaa 
      ctcggtgtg ccaactctg aatccggtt cttttcttc 
      ggagatgcg aaaaatatc ttattggga aaacggcaa 
      gttggtgtg gaatttatc gactactac aaacagttc 
      aaaccaaga ggcctaaag catggtggt gtctacctt 
      ggccctaga ccattctgg gtcatcact gatcctggt 
      ttgataaaa cacatttta caaattgat tttccccat 
      tttattaac catggcacg tacgctgat gaagaaaat 
      gatccactg agtgcacat ttgttcagt ttggaaggt 
      actaagtgg aaaaatatg cgaacgaag atgacaccg 
      actttcaca tccggaaaa atgaaaatg atgtttcaa 
      accttggtc gactgcaca ccgggtttg aaggacatc 
      atggacgaa cacgcgagt aaaaaaaaa gccgtaaat 
      atcaaaaat attttagag cgatttaca accgacatt 
      atcggttct gtcggcttt ggaattgag tgcaatagt 
      ctcaaagac ccagattcc ctattccca aaatacggg 
      aaaaaagtc ttcgactta accccagcc gaacaactt 
      aaacaaagt cttctcttt ttcctaccc aaccctgtg 
      ctgaaagca atgaagttt aggataact aacaaagat 
      gttgaagat tttttcatg aaaacggtc cgtgaaacg 
      gtgctttac agagaaaaa aacaacatc tatcgcaaa 
      gatttcatg catttgctt ctccaactt aaaaataga 
      ggaacggtg accgacgat gagaaaata attgaccac 
      agtaaccaa aaacaagaa ccagctttg actatgaac 
      gaactgacg gcccaagcg tttgtattt tttctcgcc 
      ggttttgaa acttcatca accaccatg acttttgct 
      ttatatgaa ctggcaacc aacccagac attcaagaa 
      aaattaaga aacgaaatt tgtacagta cttgaaaag 
      cacaacaac gaattaacg tataatgca attgcggaa 
      atgacgtac atggaccaa gttctgcat gaaactctg 
      agaaaatat cccccgcta ccaatttta cagagacag 
      tgcaacaag gattacatc gtcccggaa acaaatatt 
      cgcattccg aaaggcgtc gatgttgct atcacaatt 
      ttggcgttg caccgagat ccggaattt tacccaaat 
      cccgaagtt ttcgatcct gaacggttt aatgaagaa 
      aataagaat gcgagaacg ccgttcacg tggcttccg 
      tttggagac gggcccaga gtttgtatt ggattacgc 
      ttcggaatg atgcaaagt aaagtcggt cttacagct 
      ttactaaag gactacaga gtcacttta aataagaag 
      accaaagtt cccttggaa atgagcaaa aacagcttc 
      attatagcg gcagaaggc ggaatttgg ttggacatt 
      gaaaaaatt aatacctaa
    mRNA sequence
    • >TC007322 RA
      caacgaaca acttgcata caaaattaa aatgtaaaa 
      atattagta taacaccta taattaaaa aaaaacaat 
      tatattaaa attctttaa tttgttatt aaatgtaag 
      tatactaag aaattaatt acagcagaa atctagcag 
      aactaagta ccaacaaag ccaaaacta caaaaactg 
      gtttcattc tcacagtaa ataagtcag taattgtag 
      gtttcaatt atgacctcc aacaacata atgtagctg 
      ggtttttgc gccagttaa tcgagtctg atgccacca 
      atgagtcgt atattgtga aagattagc gattatgac 
      aacgccaaa gttccatat cagcacctc tcaaataag 
      agtataaaa tcaagtcat taaaaatta ataaaatca 
      gtgtcaaat caagtgaga gtgagtgtt aatatcaaa 
      agcaatagc catttgaaa aaaatggca attttcacg 
      tcaagcatt ttcatcgac ataataacc ataatcaca 
      acagtgctc gccttaacc atcgcattt ttcaaatgg 
      aaattgaca ttttgggaa aaactcggt gtgccaact 
      ctgaatccg gttcttttc ttcggagat gcgaaaaat 
      atcttattg ggaaaacgg caagttggt gtggaattt 
      atcgactac tacaaacag ttcaaacca agaggccta 
      aagcatggt ggtgtctac cttggccct agaccattc 
      tgggtcatc actgatcct ggtttgata aaacacatt 
      ttacaaatt gattttccc cattttatt aaccatggc 
      acgtacgct gatgaagaa aatgatcca ctgagtgca 
      catttgttc agtttggaa ggtactaag tggaaaaat 
      atgcgaacg aagatgaca ccgactttc acatccgga 
      aaaatgaaa atgatgttt caaaccttg gtcgactgc 
      acaccgggt ttgaaggac atcatggac gaacacgcg 
      agtaaaaaa aaagccgta aatatcaaa aatatttta 
      gagcgattt acaaccgac attatcggt tctgtcggc 
      tttggaatt gagtgcaat agtctcaaa gacccagat 
      tccctattc ccaaaatac gggaaaaaa gtcttcgac 
      ttaacccca gccgaacaa cttaaacaa agtcttctc 
      tttttccta cccaaccct gtgctgaaa gcaatgaag 
      tttaggata actaacaaa gatgttgaa gattttttc 
      atgaaaacg gtccgtgaa acggtgctt tacagagaa 
      aaaaacaac atctatcgc aaagatttc atgcatttg 
      cttctccaa cttaaaaat agaggaacg gtgaccgac 
      gatgagaaa ataattgac cacagtaac caaaaacaa 
      gaaccagct ttgactatg aacgaactg acggcccaa 
      gcgtttgta ttttttctc gccggtttt gaaacttca 
      tcaaccacc atgactttt gctttatat gaactggca 
      accaaccca gacattcaa gaaaaatta agaaacgaa 
      atttgtaca gtacttgaa aagcacaac aacgaatta 
      acgtataat gcaattgcg gaaatgacg tacatggac 
      caagttctg catgaaact ctgagaaaa tatcccccg 
      ctaccaatt ttacagaga cagtgcaac aaggattac 
      atcgtcccg gaaacaaat attcgcatt ccgaaaggc 
      gtcgatgtt gctatcaca attttggcg ttgcaccga 
      gatccggaa ttttaccca aatcccgaa gttttcgat 
      cctgaacgg tttaatgaa gaaaataag aatgcgaga 
      acgccgttc acgtggctt ccgtttgga gacgggccc 
      agagtttgt attggatta cgcttcgga atgatgcaa 
      agtaaagtc ggtcttaca gctttacta aaggactac 
      agagtcact ttaaataag aagaccaaa gttcccttg 
      gaaatgagc aaaaacagc ttcattata gcggcagaa 
      ggcggaatt tggttggac attgaaaaa attaatacc 
      taatttaaa gtgttttgt ttgtcaata aaagcgaga 
      acgttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY