Status
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Gene information
General information
TC007311 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6230593..6232416
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007311
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04312

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007311 RA
      MLLVNGSLT YDLVAILVT VLAGLVVYY KWAFTYWKK 
      RGLEYLEPS IPFGNCASL FKAKYNFGE QFAEFYKQF 
      REKNLRHGG VFMFARPFY IPVDPAIMK DIMQKDFQH 
      FVDRGFYVN EEIDPLSGH LFSLEGNKW KNLRAKLTA 
      TFTSGKMKM MFETMSRCA EELDNYVNE ASKRKETID 
      IKETLARFT TDIISSCAF GLESNCLKN PDTKFRYYG 
      KRTFQNTTW ENLKNLIQF MTPMYLLNL VKFKTTKQD 
      VEDYMLNLV KKTVDYREK NNVYRKDFL HLLLQLKNK 
      GKITDDETF LDNNNKSKE VALTINELA AQVFVFFAA 
      GYETSSTAM TFTLYELCL NQKIQDKMR EEIKTVLAK 
      HNNKISYEA LMEMTYMEQ ALNETLRKY PPVPFLNRK 
      CTKAYDVAG TNLHLDEGT MVVLPILGL QHDPEYYPD 
      PSKYDPDRF SEENKNSRP PFTWMPFGE GPRICIGLR 
      FGMLQAKVG LATLLENYK VTLSQKTKT PLEMDCKTF 
      ITTTKEGIW LDFTRLN
    CDS sequence
    • >TC007311 RA
      atgcttctc gtaaatggc agtttaaca tacgaccta 
      gtggccatt ttagtgact gtcctagcc ggactagtg 
      gtttactac aaatgggcc tttacgtat tggaaaaag 
      cgcgggctt gagtactta gaaccttca attcccttc 
      ggcaactgt gctagttta tttaaagca aaatataat 
      tttggtgag caattcgcc gaattctac aaacagttc 
      agagagaag aatctcaga catggtggc gtgtttatg 
      tttgcacgt cctttttac atccctgtg gatcctgcg 
      attatgaag gacatcatg caaaaagac tttcaacat 
      ttcgtcgat cgtggtttc tacgtaaat gaagaaatt 
      gatccttta agtgggcat ctgttttct ttggaaggc 
      aacaaatgg aaaaatctt cgagcaaaa ctgacggcc 
      acattcact tcaggtaaa atgaagatg atgtttgag 
      acaatgtca agatgtgcg gaagaactt gacaattac 
      gtgaacgaa gcttccaaa cggaaagaa accatagac 
      attaaagaa actctagca cggttcact acagatatt 
      attagttct tgtgctttt ggacttgaa agtaactgt 
      cttaaaaac ccagatacg aaattcagg tactacgga 
      aaacgtact ttccaaaat acaacttgg gagaactta 
      aaaaatcta attcaattt atgacgcct atgtatctc 
      ctcaatttg gtaaaattc aaaaccaca aaacaagat 
      gtggaagac tacatgttg aatttggtg aagaaaact 
      gttgattat agagaaaag aacaacgtt taccgaaaa 
      gatttcctc cacttgtta ctgcaattg aaaaataaa 
      ggaaaaata accgatgat gaaacattc cttgacaac 
      aacaacaaa agtaaagaa gttgcattg acaatcaat 
      gaattggcg gcacaagtt tttgtcttt ttcgccgct 
      ggttatgaa acttcatcc acggcaatg accttcact 
      ttgtacgaa ctgtgtctc aatcaaaaa atccaagac 
      aaaatgcgt gaggaaatc aagacagtg ttagcaaaa 
      cacaataac aaaatatca tacgaggct ttaatggaa 
      atgacatac atggaacaa gccctcaat gaaacgttg 
      agaaaatat ccaccggta ccattcctg aatcgaaaa 
      tgtaccaaa gcttatgac gtcgctggc acaaacctg 
      cacctcgac gaaggtaca atggttgtg cttccaatt 
      ttggggctc caacatgat ccggaatac taccctgac 
      ccatcaaaa tatgaccct gatcgcttt tcggaagaa 
      aataaaaac agtcggcca ccgttcaca tggatgcct 
      tttggtgaa ggcccaaga atttgtatt ggtttacga 
      tttggaatg ctacaagca aaagtgggt ttagccact 
      ttgttagaa aattacaaa gtgactttg agtcaaaag 
      acgaagact ccactagaa atggactgc aaaactttc 
      atcacgacc actaaagag ggaatttgg ctggatttc 
      acacgattg aattga
    mRNA sequence
    • >TC007311 RA
      gatacgaaa cgaatctca ctgactaaa taccaacaa 
      agtcaatta aaatttcgc gagtgaaaa aatgcttct 
      cgtaaatgg cagtttaac atacgacct agtggccat 
      tttagtgac tgtcctagc cggactagt ggtttacta 
      caaatgggc ctttacgta ttggaaaaa gcgcgggct 
      tgagtactt agaaccttc aattccctt cggcaactg 
      tgctagttt atttaaagc aaaatataa ttttggtga 
      gcaattcgc cgaattcta caaacagtt cagagagaa 
      gaatctcag acatggtgg cgtgtttat gtttgcacg 
      tccttttta catccctgt ggatcctgc gattatgaa 
      ggacatcat gcaaaaaga ctttcaaca tttcgtcga 
      tcgtggttt ctacgtaaa tgaagaaat tgatccttt 
      aagtgggca tctgttttc tttggaagg caacaaatg 
      gaaaaatct tcgagcaaa actgacggc cacattcac 
      ttcaggtaa aatgaagat gatgtttga gacaatgtc 
      aagatgtgc ggaagaact tgacaatta cgtgaacga 
      agcttccaa acggaaaga aaccataga cattaaaga 
      aactctagc acggttcac tacagatat tattagttc 
      ttgtgcttt tggacttga aagtaactg tcttaaaaa 
      cccagatac gaaattcag gtactacgg aaaacgtac 
      tttccaaaa tacaacttg ggagaactt aaaaaatct 
      aattcaatt tatgacgcc tatgtatct cctcaattt 
      ggtaaaatt caaaaccac aaaacaaga tgtggaaga 
      ctacatgtt gaatttggt gaagaaaac tgttgatta 
      tagagaaaa gaacaacgt ttaccgaaa agatttcct 
      ccacttgtt actgcaatt gaaaaataa aggaaaaat 
      aaccgatga tgaaacatt ccttgacaa caacaacaa 
      aagtaaaga agttgcatt gacaatcaa tgaattggc 
      ggcacaagt ttttgtctt tttcgccgc tggttatga 
      aacttcatc cacggcaat gaccttcac tttgtacga 
      actgtgtct caatcaaaa aatccaaga caaaatgcg 
      tgaggaaat caagacagt gttagcaaa acacaataa 
      caaaatatc atacgaggc tttaatgga aatgacata 
      catggaaca agccctcaa tgaaacgtt gagaaaata 
      tccaccggt accattcct gaatcgaaa atgtaccaa 
      agcttatga cgtcgctgg cacaaacct gcacctcga 
      cgaaggtac aatggttgt gcttccaat tttggggct 
      ccaacatga tccggaata ctaccctga cccatcaaa 
      atatgaccc tgatcgctt ttcggaaga aaataaaaa 
      cagtcggcc accgttcac atggatgcc ttttggtga 
      aggcccaag aatttgtat tggtttacg atttggaat 
      gctacaagc aaaagtggg tttagccac tttgttaga 
      aaattacaa agtgacttt gagtcaaaa gacgaagac 
      tccactaga aatggactg caaaacttt catcacgac 
      cactaaaga gggaatttg gctggattt cacacgatt 
      gaattgaat cttggccaa atattttgt gattgtatt 
      aaattaata ctgatattg gatttgact actgttggt 
      gtattatta atatagtat taaagtact g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY