Status
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Gene information
General information
TC007305 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6305271..6317218
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007305
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04311

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    remarks: animals lost, remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost, remarks: animals lost,
    Fertility
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007305 RA
      MDDDDEFFD TISVASGAS RRRFDNKDY LSLSRNTVY 
      QSAVDLENM ELTRLDHSE NGNSQNKRW RYFTNGELC 
      VDFVLAYDK EGKPEDVAK REHFEANLQ HVGLILEKE 
      ESQRIHFVK IHVPREVLC QYAEILKLR LPIRYDENL 
      GEIENVFYS TLNKILDCL RVRLDPKIF PPKKYRLTA 
      EFNREKNYL FDVDDPDFF NEAVRITVA SYILEREKF 
      GIEDQEKGV HRLISEGIY KAAYPLHDG DLHEKNSKR 
      VKLLKHWAQ VSKCVKYQP LDEVKEYFG IKFALYFAW 
      LGFYTHMLI PASLVGILC LVYAATTLP QDTLCKDLC 
      NSDIIMCPR CDKVCDYWK LSEGCLYAK IQHFVDNPA 
      TIFFAVFMS FWSILYLEL WKRYSAGIT HRWGLTGFD 
      LKAEPPRPE YLIRLADAK KRKLNVITN LNEPAVSFW 
      KVKLPSIIL SFTLALLWV FIAVFVVFG VVIYRMSLI 
      TSEVLYEDK ITYRIYILP ITAAIINLV CILILNIFY 
      QRLAVWLTE MELQRTQTE YDDSLALKI YMFQFVNYY 
      SSIFYIAFL KGQFVGYPA KYNRIFGFR QEECNPGGC 
      LMELTIQLA IIMIGKQAI NAVAEMVVP LLTKMYNSV 
      KVSMGIQEA PPDQIGIIS CNQWTEDYK LLDLQSQSL 
      FSEYLEMVL QYGFVTIFV TAFPLAPLF ALINNILEM 
      RLDAKKFIK YFRRPVPQR VTNIGVWFP IMGILGRIS 
      VVSNAFIIA FSSHFIPKL VYMMEVNPD HTEDGFLDS 
      TLAYFDTAD FEDGTAPEN PSMNVSICR YAEYRNPPN 
      VTNGIKYKR PLIYWHILA ARLAFVVAY QNLVTFVVT 
      AVEWTIPDI PRKLNDQIK REAYKTNET IIKYETERA 
      RLKHRKRKN RDSVLSGET VYYDANGGF HTSDLANTS 
      RGDHIENS
    • >TC007305 RB
      MDDDDEFFD TISVASGAS RRRFDNKDY LSLSRNTVY 
      QSAVDLENM ELTRLDHSE NGNSQNKVS RNRDVKTYK 
      QWRDRRWRY FTNGELCVD FVLAYDKEG KPEDVAKRE 
      HFEANLQHV GLILEKEES QRIHFVKIH VPREVLCQY 
      AEILKLRLP IRYDENLGE IENVFYSTL NKILDCLRV 
      RLDPKIFPP KKYRLTAEF NREKNYLFD VDDPDFFNE 
      AVRITVASY ILEREKFGI EDQEKGVHR LISEGIYKA 
      AYPLHDGDL HEKNSKRVK LLKHWAQVS KCVKYQPLD 
      EVKEYFGIK FALYFAWLG FYTHMLIPA SLVGILCLV 
      YAATTLPQD TLCKDLCNS DIIMCPRCD KVCDYWKLS 
      EGCLYAKIQ HFVDNPATI FFAVFMSFW SILYLELWK 
      RYSAGITHR WGLTGFDLK AEPPRPEYL IRLADAKKR 
      KLNVITNLN EPAVSFWKV KLPSIILSF TLALLWVFI 
      AVFVVFGVV IYRMSLITS EVLYEDKIT YRIYILPIT 
      AAIINLVCI LILNIFYQR LAVWLTEME LQRTQTEYD 
      DSLALKIYM FQFVNYYSS IFYIAFLKG QFVGYPAKY 
      NRIFGFRQE ECNPGGCLM ELTIQLAII MIGKQAINA 
      VAEMVVPLL TKMYNSVKV SMGIQEAPP DQIGIISCN 
      QWTEDYKLL DLQSQSLFS EYLEMVLQY GFVTIFVTA 
      FPLAPLFAL INNILEMRL DAKKFIKYF RRPVPQRVT 
      NIGVWFPIM GILGRISVV SNAFIIAFS SHFIPKLVY 
      MMEVNPDHT EDGFLDSTL AYFDTADFE DGTAPENPS 
      MNVSICRYA EYRNPPNVT NGIKYKRPL IYWHILAAR 
      LAFVVAYQN LVTFVVTAV EWTIPDIPR KLNDQIKRE 
      AYKTNETII KYETERARL KHRKRKNRD SVLSGETVY 
      YDANGGFHT SDLANTSRG DHIENS
    CDS sequence
    • >TC007305 RA
      atggatgac gatgatgaa tttttcgat acgatctca 
      gttgcttca ggagcgagt cgaaggcga tttgataat 
      aaagattac ctcagttta tcaagaaac acagtgtat 
      cagtcggcc gttgattta gaaaatatg gaactgaca 
      cgtctggac cattcggaa aatgggaac tcacaaaat 
      aagcgctgg cgttacttc acaaacggc gaattgtgc 
      gtcgatttc gtgttggct tacgacaaa gaaggcaag 
      cctgaagat gtggcaaag cgagaacac ttcgaagca 
      aatctacag catgtaggt cttattttg gaaaaggag 
      gaaagccag cgcattcat ttcgtcaaa attcacgtg 
      ccgagggaa gtcctgtgc caatatgcg gaaatttta 
      aaattaaga ctaccaatt agatacgat gaaaattta 
      ggcgaaatc gaaaatgtg ttttactcg acgttgaac 
      aagatactg gactgttta cgagtgaga ttggaccct 
      aaaatattc ccgccgaag aagtacaga ctgacggct 
      gaattcaac agggagaaa aactacttg ttcgatgtg 
      gacgatcct gattttttc aacgaagcg gttcggatt 
      actgtggct tcgtatatt ttagaacgc gaaaaattc 
      ggaatagag gaccaagaa aagggagtt catagactg 
      atttcagag ggcatttac aaggctgcc tatccgcta 
      catgatggc gatctgcac gagaaaaac tccaaacgc 
      gtcaaactc ctaaaacac tgggcccaa gtgagcaaa 
      tgcgtgaaa taccaaccc ctcgacgaa gtgaaagaa 
      tatttcggc ataaaattc gcattatac ttcgcatgg 
      ctcggtttc tacacccac atgttgatc ccggcatca 
      cttgtggga atcctctgt ctagtctat gccgcaacg 
      actttaccg caagacact ttatgcaaa gacttgtgc 
      aattcggac atcattatg tgcccccga tgcgataaa 
      gtttgcgac tattggaaa ttgagcgag ggctgtctt 
      tatgccaaa atccagcac tttgtcgac aaccccgcc 
      acgattttc ttcgccgtt ttcatgtcc ttttggtcg 
      attttgtac ctagaactg tggaaacgc tacagtgct 
      ggtatcacc catcgctgg ggcctcaca gggttcgat 
      ttgaaagct gagccgccc agacccgag tatttgatc 
      cgcttagcc gacgccaaa aagcgaaaa ctgaacgtc 
      atcacgaat ttaaacgaa ccggctgtg tctttttgg 
      aaagttaaa ctaccctcg attattcta agcttcacg 
      cttgcccta ttgtgggtt tttatcgca gtttttgtg 
      gttttcggc gttgttatt tatcggatg tccctaatc 
      acgtcggaa gttctctac gaggataaa atcacttac 
      cggatttat attctacca atcactgcg gccattatt 
      aatttagtg tgtattcta attttgaac attttctac 
      caacgattg gccgtctgg ttgacggaa atggagctg 
      caacggacg caaaccgaa tacgatgac agtctagcg 
      ctgaaaatt tacatgttt cagtttgtt aattactat 
      tcttcgatt ttttatatc gcgtttttg aaaggtcag 
      tttgtggga tatccggct aagtataat cggattttc 
      gggttcagg caagaagag tgtaacccg ggagggtgt 
      ttaatggag cttactatt caactagcc attattatg 
      atcggtaaa caggcgatt aatgcagtt gccgaaatg 
      gttgtgcct ttattaacg aaaatgtac aattcggtc 
      aaagtttca atggggatt caggaagcg cctccagac 
      caaattggg attatcagc tgcaatcag tggaccgaa 
      gactacaaa ttgttggat cttcagtca caaagcttg 
      ttttcggag tatttagaa atggttttg cagtacggt 
      ttcgtaacg attttcgtg accgccttc cctctggcc 
      cccctgttc gcgcttatc aacaacatc ctggaaatg 
      cgtctcgac gcgaaaaaa ttcatcaaa tatttccgg 
      cgccctgtc ccgcaacga gtgacaaac atcggcgtt 
      tggttcccc ataatgggg atccttggc cgaatttct 
      gtcgtttcg aacgctttt atcatcgct ttttcgtca 
      cattttatc cccaaactc gtatatatg atggaggtg 
      aaccccgac cacaccgag gacgggttc cttgacagc 
      actttggcc tattttgac actgccgat tttgaagat 
      gggaccgcg cctgaaaac ccgtctatg aacgtatcg 
      atttgtcga tacgccgaa tacaggaac cccccaaac 
      gtcacgaat ggaatcaag tataaacgg ccgttgatt 
      tattggcat attttggcc gcgcggttg gcttttgtg 
      gtggcgtat cagaacttg gtcactttt gttgtgact 
      gcagtggaa tggacaatt ccagacatt ccgaggaag 
      ttgaatgat cagatcaag agagaagct tacaagact 
      aacgaaacc attatcaag tacgaaact gaacgggcc 
      cgtttaaaa cacagaaaa aggaaaaat cgtgactcg 
      gtcctgtca ggtgagaca gtttattat gacgccaat 
      ggaggcttc catacttca gacctagca aacacctca 
      aggggggac cacatagag aattcttag
    • >TC007305 RB
      atggatgac gatgatgaa tttttcgat acgatctca 
      gttgcttca ggagcgagt cgaaggcga tttgataat 
      aaagattac ctcagttta tcaagaaac acagtgtat 
      cagtcggcc gttgattta gaaaatatg gaactgaca 
      cgtctggac cattcggaa aatgggaac tcacaaaat 
      aaggtgtct agaaataga gatgttaag acgtacaag 
      cagtggaga gataggcgc tggcgttac ttcacaaac 
      ggcgaattg tgcgtcgat ttcgtgttg gcttacgac 
      aaagaaggc aagcctgaa gatgtggca aagcgagaa 
      cacttcgaa gcaaatcta cagcatgta ggtcttatt 
      ttggaaaag gaggaaagc cagcgcatt catttcgtc 
      aaaattcac gtgccgagg gaagtcctg tgccaatat 
      gcggaaatt ttaaaatta agactacca attagatac 
      gatgaaaat ttaggcgaa atcgaaaat gtgttttac 
      tcgacgttg aacaagata ctggactgt ttacgagtg 
      agattggac cctaaaata ttcccgccg aagaagtac 
      agactgacg gctgaattc aacagggag aaaaactac 
      ttgttcgat gtggacgat cctgatttt ttcaacgaa 
      gcggttcgg attactgtg gcttcgtat attttagaa 
      cgcgaaaaa ttcggaata gaggaccaa gaaaaggga 
      gttcataga ctgatttca gagggcatt tacaaggct 
      gcctatccg ctacatgat ggcgatctg cacgagaaa 
      aactccaaa cgcgtcaaa ctcctaaaa cactgggcc 
      caagtgagc aaatgcgtg aaataccaa cccctcgac 
      gaagtgaaa gaatatttc ggcataaaa ttcgcatta 
      tacttcgca tggctcggt ttctacacc cacatgttg 
      atcccggca tcacttgtg ggaatcctc tgtctagtc 
      tatgccgca acgacttta ccgcaagac actttatgc 
      aaagacttg tgcaattcg gacatcatt atgtgcccc 
      cgatgcgat aaagtttgc gactattgg aaattgagc 
      gagggctgt ctttatgcc aaaatccag cactttgtc 
      gacaacccc gccacgatt ttcttcgcc gttttcatg 
      tccttttgg tcgattttg tacctagaa ctgtggaaa 
      cgctacagt gctggtatc acccatcgc tggggcctc 
      acagggttc gatttgaaa gctgagccg cccagaccc 
      gagtatttg atccgctta gccgacgcc aaaaagcga 
      aaactgaac gtcatcacg aatttaaac gaaccggct 
      gtgtctttt tggaaagtt aaactaccc tcgattatt 
      ctaagcttc acgcttgcc ctattgtgg gtttttatc 
      gcagttttt gtggttttc ggcgttgtt atttatcgg 
      atgtcccta atcacgtcg gaagttctc tacgaggat 
      aaaatcact taccggatt tatattcta ccaatcact 
      gcggccatt attaattta gtgtgtatt ctaattttg 
      aacattttc taccaacga ttggccgtc tggttgacg 
      gaaatggag ctgcaacgg acgcaaacc gaatacgat 
      gacagtcta gcgctgaaa atttacatg tttcagttt 
      gttaattac tattcttcg attttttat atcgcgttt 
      ttgaaaggt cagtttgtg ggatatccg gctaagtat 
      aatcggatt ttcgggttc aggcaagaa gagtgtaac 
      ccgggaggg tgtttaatg gagcttact attcaacta 
      gccattatt atgatcggt aaacaggcg attaatgca 
      gttgccgaa atggttgtg cctttatta acgaaaatg 
      tacaattcg gtcaaagtt tcaatgggg attcaggaa 
      gcgcctcca gaccaaatt gggattatc agctgcaat 
      cagtggacc gaagactac aaattgttg gatcttcag 
      tcacaaagc ttgttttcg gagtattta gaaatggtt 
      ttgcagtac ggtttcgta acgattttc gtgaccgcc 
      ttccctctg gcccccctg ttcgcgctt atcaacaac 
      atcctggaa atgcgtctc gacgcgaaa aaattcatc 
      aaatatttc cggcgccct gtcccgcaa cgagtgaca 
      aacatcggc gtttggttc cccataatg gggatcctt 
      ggccgaatt tctgtcgtt tcgaacgct tttatcatc 
      gctttttcg tcacatttt atccccaaa ctcgtatat 
      atgatggag gtgaacccc gaccacacc gaggacggg 
      ttccttgac agcactttg gcctatttt gacactgcc 
      gattttgaa gatgggacc gcgcctgaa aacccgtct 
      atgaacgta tcgatttgt cgatacgcc gaatacagg 
      aacccccca aacgtcacg aatggaatc aagtataaa 
      cggccgttg atttattgg catattttg gccgcgcgg 
      ttggctttt gtggtggcg tatcagaac ttggtcact 
      tttgttgtg actgcagtg gaatggaca attccagac 
      attccgagg aagttgaat gatcagatc aagagagaa 
      gcttacaag actaacgaa accattatc aagtacgaa 
      actgaacgg gcccgttta aaacacaga aaaaggaaa 
      aatcgtgac tcggtcctg tcaggtgag acagtttat 
      tatgacgcc aatggaggc ttccatact tcagaccta 
      gcaaacacc tcaaggggg gaccacata gagaattct 
      tag
    mRNA sequence
    • >TC007305 RA
      aagatagtt gttttagta aagtttggt taggtaagg 
      agcgagtgt tgttatcag gtgctgtgt gataagtga 
      tatgataac atgtagtga tcactgcga tagcacgta 
      acatcacaa ccatgcatg agccgcata ttctagcac 
      attgcacca gtgggaaaa tgatagaga taatccgta 
      atggatgac gatgatgaa tttttcgat acgatctca 
      gttgcttca ggagcgagt cgaaggcga tttgataat 
      aaagattac ctcagttta tcaagaaac acagtgtat 
      cagtcggcc gttgattta gaaaatatg gaactgaca 
      cgtctggac cattcggaa aatgggaac tcacaaaat 
      aagcgctgg cgttacttc acaaacggc gaattgtgc 
      gtcgatttc gtgttggct tacgacaaa gaaggcaag 
      cctgaagat gtggcaaag cgagaacac ttcgaagca 
      aatctacag catgtaggt cttattttg gaaaaggag 
      gaaagccag cgcattcat ttcgtcaaa attcacgtg 
      ccgagggaa gtcctgtgc caatatgcg gaaatttta 
      aaattaaga ctaccaatt agatacgat gaaaattta 
      ggcgaaatc gaaaatgtg ttttactcg acgttgaac 
      aagatactg gactgttta cgagtgaga ttggaccct 
      aaaatattc ccgccgaag aagtacaga ctgacggct 
      gaattcaac agggagaaa aactacttg ttcgatgtg 
      gacgatcct gattttttc aacgaagcg gttcggatt 
      actgtggct tcgtatatt ttagaacgc gaaaaattc 
      ggaatagag gaccaagaa aagggagtt catagactg 
      atttcagag ggcatttac aaggctgcc tatccgcta 
      catgatggc gatctgcac gagaaaaac tccaaacgc 
      gtcaaactc ctaaaacac tgggcccaa gtgagcaaa 
      tgcgtgaaa taccaaccc ctcgacgaa gtgaaagaa 
      tatttcggc ataaaattc gcattatac ttcgcatgg 
      ctcggtttc tacacccac atgttgatc ccggcatca 
      cttgtggga atcctctgt ctagtctat gccgcaacg 
      actttaccg caagacact ttatgcaaa gacttgtgc 
      aattcggac atcattatg tgcccccga tgcgataaa 
      gtttgcgac tattggaaa ttgagcgag ggctgtctt 
      tatgccaaa atccagcac tttgtcgac aaccccgcc 
      acgattttc ttcgccgtt ttcatgtcc ttttggtcg 
      attttgtac ctagaactg tggaaacgc tacagtgct 
      ggtatcacc catcgctgg ggcctcaca gggttcgat 
      ttgaaagct gagccgccc agacccgag tatttgatc 
      cgcttagcc gacgccaaa aagcgaaaa ctgaacgtc 
      atcacgaat ttaaacgaa ccggctgtg tctttttgg 
      aaagttaaa ctaccctcg attattcta agcttcacg 
      cttgcccta ttgtgggtt tttatcgca gtttttgtg 
      gttttcggc gttgttatt tatcggatg tccctaatc 
      acgtcggaa gttctctac gaggataaa atcacttac 
      cggatttat attctacca atcactgcg gccattatt 
      aatttagtg tgtattcta attttgaac attttctac 
      caacgattg gccgtctgg ttgacggaa atggagctg 
      caacggacg caaaccgaa tacgatgac agtctagcg 
      ctgaaaatt tacatgttt cagtttgtt aattactat 
      tcttcgatt ttttatatc gcgtttttg aaaggtcag 
      tttgtggga tatccggct aagtataat cggattttc 
      gggttcagg caagaagag tgtaacccg ggagggtgt 
      ttaatggag cttactatt caactagcc attattatg 
      atcggtaaa caggcgatt aatgcagtt gccgaaatg 
      gttgtgcct ttattaacg aaaatgtac aattcggtc 
      aaagtttca atggggatt caggaagcg cctccagac 
      caaattggg attatcagc tgcaatcag tggaccgaa 
      gactacaaa ttgttggat cttcagtca caaagcttg 
      ttttcggag tatttagaa atggttttg cagtacggt 
      ttcgtaacg attttcgtg accgccttc cctctggcc 
      cccctgttc gcgcttatc aacaacatc ctggaaatg 
      cgtctcgac gcgaaaaaa ttcatcaaa tatttccgg 
      cgccctgtc ccgcaacga gtgacaaac atcggcgtt 
      tggttcccc ataatgggg atccttggc cgaatttct 
      gtcgtttcg aacgctttt atcatcgct ttttcgtca 
      cattttatc cccaaactc gtatatatg atggaggtg 
      aaccccgac cacaccgag gacgggttc cttgacagc 
      actttggcc tattttgac actgccgat tttgaagat 
      gggaccgcg cctgaaaac ccgtctatg aacgtatcg 
      atttgtcga tacgccgaa tacaggaac cccccaaac 
      gtcacgaat ggaatcaag tataaacgg ccgttgatt 
      tattggcat attttggcc gcgcggttg gcttttgtg 
      gtggcgtat cagaacttg gtcactttt gttgtgact 
      gcagtggaa tggacaatt ccagacatt ccgaggaag 
      ttgaatgat cagatcaag agagaagct tacaagact 
      aacgaaacc attatcaag tacgaaact gaacgggcc 
      cgtttaaaa cacagaaaa aggaaaaat cgtgactcg 
      gtcctgtca ggtgagaca gtttattat gacgccaat 
      ggaggcttc catacttca gacctagca aacacctca 
      aggggggac cacatagag aattcttag cacacgccg 
      atatttttt attacttgt acataacat ttggaccaa 
      ggagatcag tctcccatt aaggccttt tgacttgcc 
      ttcaactgc tacggcttt aatacttat taacatttc 
      aatggcaaa cctcaatac tcagaaaag tgataattt 
      cctcgccgt ttgagcttt aaatgacag tgatttcca 
      aaatatcac aacttttca aatttgaac aaatggtgc 
      ctgtacggt tggaactaa cttatttga atttttaac 
      aattaaaac acggattaa aacagttac ttaatttgt 
      ccaaattcg gaaggttgt gctactaat ttcaactgt 
      aaaaaactc aaaattaat tatcaaaac tttcaatat 
      ttgcaaaaa tccttgtta gtttataat tgttatatc 
      tcaaatcgt tgataatta cttcaaatc taggcgttt 
      taaataaat tttattcaa gtgttaaat taatatatt 
      tttataaat cttagttaa tacgagaca tgtacaaaa 
      ttaaagaat caaaacgtt g
    • >TC007305 RB
      aagatagtt gttttagta aagtttggt taggtaagg 
      agcgagtgt tgttatcag gtgctgtgt gataagtga 
      tatgataac atgtagtga tcactgcga tagcacgta 
      acatcacaa ccatgcatg agccgcata ttctagcac 
      attgcacca gtgggaaaa tgatagaga taatccgta 
      atggatgac gatgatgaa tttttcgat acgatctca 
      gttgcttca ggagcgagt cgaaggcga tttgataat 
      aaagattac ctcagttta tcaagaaac acagtgtat 
      cagtcggcc gttgattta gaaaatatg gaactgaca 
      cgtctggac cattcggaa aatgggaac tcacaaaat 
      aaggtgtct agaaataga gatgttaag acgtacaag 
      cagtggaga gataggcgc tggcgttac ttcacaaac 
      ggcgaattg tgcgtcgat ttcgtgttg gcttacgac 
      aaagaaggc aagcctgaa gatgtggca aagcgagaa 
      cacttcgaa gcaaatcta cagcatgta ggtcttatt 
      ttggaaaag gaggaaagc cagcgcatt catttcgtc 
      aaaattcac gtgccgagg gaagtcctg tgccaatat 
      gcggaaatt ttaaaatta agactacca attagatac 
      gatgaaaat ttaggcgaa atcgaaaat gtgttttac 
      tcgacgttg aacaagata ctggactgt ttacgagtg 
      agattggac cctaaaata ttcccgccg aagaagtac 
      agactgacg gctgaattc aacagggag aaaaactac 
      ttgttcgat gtggacgat cctgatttt ttcaacgaa 
      gcggttcgg attactgtg gcttcgtat attttagaa 
      cgcgaaaaa ttcggaata gaggaccaa gaaaaggga 
      gttcataga ctgatttca gagggcatt tacaaggct 
      gcctatccg ctacatgat ggcgatctg cacgagaaa 
      aactccaaa cgcgtcaaa ctcctaaaa cactgggcc 
      caagtgagc aaatgcgtg aaataccaa cccctcgac 
      gaagtgaaa gaatatttc ggcataaaa ttcgcatta 
      tacttcgca tggctcggt ttctacacc cacatgttg 
      atcccggca tcacttgtg ggaatcctc tgtctagtc 
      tatgccgca acgacttta ccgcaagac actttatgc 
      aaagacttg tgcaattcg gacatcatt atgtgcccc 
      cgatgcgat aaagtttgc gactattgg aaattgagc 
      gagggctgt ctttatgcc aaaatccag cactttgtc 
      gacaacccc gccacgatt ttcttcgcc gttttcatg 
      tccttttgg tcgattttg tacctagaa ctgtggaaa 
      cgctacagt gctggtatc acccatcgc tggggcctc 
      acagggttc gatttgaaa gctgagccg cccagaccc 
      gagtatttg atccgctta gccgacgcc aaaaagcga 
      aaactgaac gtcatcacg aatttaaac gaaccggct 
      gtgtctttt tggaaagtt aaactaccc tcgattatt 
      ctaagcttc acgcttgcc ctattgtgg gtttttatc 
      gcagttttt gtggttttc ggcgttgtt atttatcgg 
      atgtcccta atcacgtcg gaagttctc tacgaggat 
      aaaatcact taccggatt tatattcta ccaatcact 
      gcggccatt attaattta gtgtgtatt ctaattttg 
      aacattttc taccaacga ttggccgtc tggttgacg 
      gaaatggag ctgcaacgg acgcaaacc gaatacgat 
      gacagtcta gcgctgaaa atttacatg tttcagttt 
      gttaattac tattcttcg attttttat atcgcgttt 
      ttgaaaggt cagtttgtg ggatatccg gctaagtat 
      aatcggatt ttcgggttc aggcaagaa gagtgtaac 
      ccgggaggg tgtttaatg gagcttact attcaacta 
      gccattatt atgatcggt aaacaggcg attaatgca 
      gttgccgaa atggttgtg cctttatta acgaaaatg 
      tacaattcg gtcaaagtt tcaatgggg attcaggaa 
      gcgcctcca gaccaaatt gggattatc agctgcaat 
      cagtggacc gaagactac aaattgttg gatcttcag 
      tcacaaagc ttgttttcg gagtattta gaaatggtt 
      ttgcagtac ggtttcgta acgattttc gtgaccgcc 
      ttccctctg gcccccctg ttcgcgctt atcaacaac 
      atcctggaa atgcgtctc gacgcgaaa aaattcatc 
      aaatatttc cggcgccct gtcccgcaa cgagtgaca 
      aacatcggc gtttggttc cccataatg gggatcctt 
      ggccgaatt tctgtcgtt tcgaacgct tttatcatc 
      gctttttcg tcacatttt atccccaaa ctcgtatat 
      atgatggag gtgaacccc gaccacacc gaggacggg 
      ttccttgac agcactttg gcctatttt gacactgcc 
      gattttgaa gatgggacc gcgcctgaa aacccgtct 
      atgaacgta tcgatttgt cgatacgcc gaatacagg 
      aacccccca aacgtcacg aatggaatc aagtataaa 
      cggccgttg atttattgg catattttg gccgcgcgg 
      ttggctttt gtggtggcg tatcagaac ttggtcact 
      tttgttgtg actgcagtg gaatggaca attccagac 
      attccgagg aagttgaat gatcagatc aagagagaa 
      gcttacaag actaacgaa accattatc aagtacgaa 
      actgaacgg gcccgttta aaacacaga aaaaggaaa 
      aatcgtgac tcggtcctg tcaggtgag acagtttat 
      tatgacgcc aatggaggc ttccatact tcagaccta 
      gcaaacacc tcaaggggg gaccacata gagaattct 
      tagcacacg ccgatattt tttattact tgtacataa 
      catttggac caaggagat cagtctccc attaaggcc 
      ttttgactt gccttcaac tgctacggc tttaatact 
      tattaacat ttcaatggc aaacctcaa tactcagaa 
      aagtgataa tttcctcgc cgtttgagc tttaaatga 
      cagtgattt ccaaaatat cacaacttt tcaaatttg 
      aacaaatgg tgcctgtac ggttggaac taacttatt 
      tgaattttt aacaattaa aacacggat taaaacagt 
      tacttaatt tgtccaaat tcggaaggt tgtgctact 
      aatttcaac tgtaaaaaa ctcaaaatt aattatcaa 
      aactttcaa tatttgcaa aaatccttg ttagtttat 
      aattgttat atctcaaat cgttgataa ttacttcaa 
      atctaggcg ttttaaata aattttatt caagtgtta 
      aattaatat atttttata aatcttagt taatacgag 
      acatgtaca aaattaaag aatcaaaac gttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY