Status
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Gene information
General information
TC007300 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6358600..6366257
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007300
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04309

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007300 RA
      MSWWLLTCL LTVWILTST GEERKLHRL VGGHLRIYH 
      EDPSDAELL EALASGSKN KKNQKNDDE EKKTLSQQV 
      ADGKYGLIQ KELFAKPSK RPGVLSYDS NPEVPKDNI 
      NNLGGLTKN EIWLAENHL LVLKGGTYP PHDDKTDNS 
      ATSWPPIDD YKAPHRQVK IPAHPKVPP PFPVQLTDD 
      GPLQILGTN SSRTLNGTF DQTAFAVPP PEGFDPGPY 
      LPPNHQYSS NATPGQPPF PIPAYPSGE YLPGAPFPP 
      YNLNGTLPP FFASLPPGA AILPPPNQT EPFDEDDPS 
      IYYPPPYSF YYPKDNSSA VPAGPLVPG IILPPPPDF 
      FGPLEETTK PTRHRMKTT TTPPPQIHP TTTAVPPTS 
      VLKTRKPIK ILPVHIPAE INNELKPIV TTSAPKLVT 
      ILPKNRTTQ KTRPPSVTI LKPVKPPSA PPKIYVYGN 
      EYSVYKPPN RSKTVISTT QVPLKVYYS TSNEIETNS 
      ITHAPERYH IGTRRAKTT TKSPTQYYY YEEQPKEVT 
      TRKPYYDIP KEYYIPAKQ VQQPKFIYV PTRPQRPRY 
      RFVQTPKTD TFSIHIARL QKQIHQYYT TPRPTYNRP 
      SPKPVYQFS FEASNYRPQ RPQIEDPQD KFRPLPKYS 
      VQIQPAIEI IPTEAPTER PNYEYAENP KPAPTPRPI 
      SQFAFVTPN PVYQGFYTK PDEGFFDEN TKQYFTTFG 
      QKLAPGTTP LPPRRPPKP SLEGDTAVN FLPPRPTIN 
      PDAEFVPIN PNQQRVNYP QGVNYPQVV KYPRPVQYA 
      QETRQQNRE TVKNEGRDG SFISYQLPG DDGAHFYFL 
      TPQLAQSRA QGAGYYYSQ PRIRRNKER
    CDS sequence
    • >TC007300 RA
      atgtcctgg tggcttctc acgtgccta ctaacagta 
      tggattcta acttccacg ggagaagaa agaaaactt 
      cacagatta gtcggaggc catttgaga atttatcat 
      gaagatccc tccgatgct gaactgctc gaagcatta 
      gcaagtggt tcaaaaaat aagaaaaat cagaaaaat 
      gacgacgag gagaagaaa acactgagc caacaagtc 
      gctgatggg aaatacgga ctgatccaa aaagaactt 
      ttcgcaaaa ccgtcgaaa cgtcctggg gtacttagt 
      tacgacagt aaccctgaa gtccccaaa gacaatatt 
      aataatttg ggtggactg accaaaaac gagatttgg 
      ttggcggaa aaccacctt ctagtcctc aaaggtggt 
      acttatccg ccccatgat gataaaacg gacaattcg 
      gcgacgtcc tggcccccg atcgacgac tacaaggcg 
      ccccatcgt caagtcaaa atccccgca caccccaaa 
      gtaccgccg ccatttccc gtccaactc accgacgac 
      ggccccttg caaatcctg ggcacaaat tcttcgcga 
      acgcttaat ggcacgttc gaccaaacg gcctttgcc 
      gtacctccc ccagaaggc ttcgacccg ggtccgtac 
      ctcccgcct aaccatcaa tattcgtcc aacgctact 
      cccgggcaa cctccgttc ccaatacct gcgtaccct 
      tcgggggag taccttccg ggggcaccg ttccccccg 
      tacaacttg aacggtact ttacctcct tttttcgct 
      tctttaccc ccgggagcc gccatacta ccaccaccg 
      aaccaaaca gaaccgttc gacgaagac gacccttcg 
      atctactat cctccgcct tacagtttc tactaccca 
      aaggataac tcatcggcg gtaccagcc ggacctcta 
      gtacccgga attattcta ccgccccca ccggacttt 
      ttcggtcct ttggaggaa acaacgaaa ccaacaaga 
      cacagaatg aaaaccacg accactccg ccaccacaa 
      attcaccca actacgaca gcggtaccg ccaacttcg 
      gtacttaaa acacggaaa ccgatcaaa atacttccg 
      gtgcatatc ccggctgaa attaacaac gaattgaaa 
      ccaatagtg acgacaagt gcgccgaaa cttgttaca 
      attctcccg aaaaatcgt accactcaa aagacgcgt 
      ccaccaagt gtgactatt ttaaaacca gtgaaaccg 
      cccagtgct cctcctaaa atttacgtg tacggtaac 
      gaatattcc gtttacaaa ccaccaaat cgctccaaa 
      actgtcatc agtacgact caagtgcca ctcaaagtg 
      tactacagt acttcgaac gaaatcgaa acaaattca 
      ataacacac gcgccggaa cggtaccac attggtacc 
      cgacgtgcc aaaacaaca acaaaatca ccaacacag 
      tattactac tacgaggaa caaccaaaa gaagtcacc 
      acacgtaaa ccgtactac gacataccc aaagagtac 
      tacattccg gcaaaacaa gtccaacaa ccgaaattt 
      atttacgtg ccaacgcgc ccccaaaga ccacgctat 
      cggtttgtg caaaccccc aaaaccgac actttcagc 
      atacacatc gcaagactc cagaaacaa atccaccaa 
      tactacaca accccccgc ccgacttac aacagaccg 
      tccccaaaa cccgtctat caattcagc ttcgaagcg 
      tccaactac cggccccaa agaccccaa atcgaagac 
      ccccaagac aaattccgc ccgttaccg aaatacagt 
      gtccaaatc cagcccgca attgaaatc atcccaacg 
      gaagctccc accgaacga ccgaactac gaatacgcc 
      gaaaatccc aaacctgcg ccaacgcca cgcccgatt 
      agccagttc gccttcgtg acccctaat ccagtctat 
      cagggtttc tataccaaa cctgacgaa ggtttcttt 
      gacgaaaac acgaagcaa tattttaca acgtttggg 
      caaaaactg gcacctggt accacaccg ttgccgcca 
      agacggccc ccgaaaccc agtcttgaa ggcgacacc 
      gctgttaat ttccttcct ccacgtccc accatcaac 
      ccggacgcc gaattcgtg ccaattaac cccaaccaa 
      caacgagtc aactacccc caaggagtc aactacccc 
      caagtggtc aagtacccg cgacctgtt caatacgct 
      caagagacg cgtcaacaa aaccgcgaa actgttaaa 
      aatgaagga cgggacggt tccttcatt tcgtatcaa 
      ttgccggga gatgacggc gctcatttt tactttttg 
      acgccacaa ttggcccaa agtcgggca caaggtgca 
      gggtattac tactcacaa ccgagaata cgacgaaat 
      aaagaaagg tga
    mRNA sequence
    • >TC007300 RA
      taaacaccc aataaacta cagagttcg tgtccgaat 
      gtcctggtg gcttctcac gtgcctact aacagtatg 
      gattctaac ttccacggg agaagaaag aaaacttca 
      cagattagt cggaggcca tttgagaat ttatcatga 
      agatccctc cgatgctga actgctcga agcattagc 
      aagtggttc aaaaaataa gaaaaatca gaaaaatga 
      cgacgagga gaagaaaac actgagcca acaagtcgc 
      tgatgggaa atacggact gatccaaaa agaactttt 
      cgcaaaacc gtcgaaacg tcctggggt acttagtta 
      cgacagtaa ccctgaagt ccccaaaga caatattaa 
      taatttggg tggactgac caaaaacga gatttggtt 
      ggcggaaaa ccaccttct agtcctcaa aggtggtac 
      ttatccgcc ccatgatga taaaacgga caattcggc 
      gacgtcctg gcccccgat cgacgacta caaggcgcc 
      ccatcgtca agtcaaaat ccccgcaca ccccaaagt 
      accgccgcc atttcccgt ccaactcac cgacgacgg 
      ccccttgca aatcctggg cacaaattc ttcgcgaac 
      gcttaatgg cacgttcga ccaaacggc ctttgccgt 
      acctccccc agaaggctt cgacccggg tccgtacct 
      cccgcctaa ccatcaata ttcgtccaa cgctactcc 
      cgggcaacc tccgttccc aatacctgc gtacccttc 
      gggggagta ccttccggg ggcaccgtt ccccccgta 
      caacttgaa cggtacttt acctccttt tttcgcttc 
      tttaccccc gggagccgc catactacc accaccgaa 
      ccaaacaga accgttcga cgaagacga cccttcgat 
      ctactatcc tccgcctta cagtttcta ctacccaaa 
      ggataactc atcggcggt accagccgg acctctagt 
      acccggaat tattctacc gcccccacc ggacttttt 
      cggtccttt ggaggaaac aacgaaacc aacaagaca 
      cagaatgaa aaccacgac cactccgcc accacaaat 
      tcacccaac tacgacagc ggtaccgcc aacttcggt 
      acttaaaac acggaaacc gatcaaaat acttccggt 
      gcatatccc ggctgaaat taacaacga attgaaacc 
      aatagtgac gacaagtgc gccgaaact tgttacaat 
      tctcccgaa aaatcgtac cactcaaaa gacgcgtcc 
      accaagtgt gactatttt aaaaccagt gaaaccgcc 
      cagtgctcc tcctaaaat ttacgtgta cggtaacga 
      atattccgt ttacaaacc accaaatcg ctccaaaac 
      tgtcatcag tacgactca agtgccact caaagtgta 
      ctacagtac ttcgaacga aatcgaaac aaattcaat 
      aacacacgc gccggaacg gtaccacat tggtacccg 
      acgtgccaa aacaacaac aaaatcacc aacacagta 
      ttactacta cgaggaaca accaaaaga agtcaccac 
      acgtaaacc gtactacga catacccaa agagtacta 
      cattccggc aaaacaagt ccaacaacc gaaatttat 
      ttacgtgcc aacgcgccc ccaaagacc acgctatcg 
      gtttgtgca aacccccaa aaccgacac tttcagcat 
      acacatcgc aagactcca gaaacaaat ccaccaata 
      ctacacaac cccccgccc gacttacaa cagaccgtc 
      cccaaaacc cgtctatca attcagctt cgaagcgtc 
      caactaccg gccccaaag accccaaat cgaagaccc 
      ccaagacaa attccgccc gttaccgaa atacagtgt 
      ccaaatcca gcccgcaat tgaaatcat cccaacgga 
      agctcccac cgaacgacc gaactacga atacgccga 
      aaatcccaa acctgcgcc aacgccacg cccgattag 
      ccagttcgc cttcgtgac ccctaatcc agtctatca 
      gggtttcta taccaaacc tgacgaagg tttctttga 
      cgaaaacac gaagcaata ttttacaac gtttgggca 
      aaaactggc acctggtac cacaccgtt gccgccaag 
      acggccccc gaaacccag tcttgaagg cgacaccgc 
      tgttaattt ccttcctcc acgtcccac catcaaccc 
      ggacgccga attcgtgcc aattaaccc caaccaaca 
      acgagtcaa ctaccccca aggagtcaa ctaccccca 
      agtggtcaa gtacccgcg acctgttca atacgctca 
      agagacgcg tcaacaaaa ccgcgaaac tgttaaaaa 
      tgaaggacg ggacggttc cttcatttc gtatcaatt 
      gccgggaga tgacggcgc tcattttta ctttttgac 
      gccacaatt ggcccaaag tcgggcaca aggtgcagg 
      gtattacta ctcacaacc gagaatacg acgaaataa 
      agaaaggtg aagtgttag tgacttgat attgacaga 
      gctgtgagt ttgtggcga t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY