Status
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Gene information
General information
TC007298 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6395200..6420617
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007298
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04308

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    Pupal morphology
    abdominal segment 8 ventral medianspotted - Number of animals showing the phenotype: 3

    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007298 RA
      MPPLVEHYH FLTSMEPPR NGDMVSSVH VGERTHRTA 
NPITPALWT ALYDYDAQG EDELSLRKG QIVEVLSED 
AKISGDEGW WTGKIGDKV GIFPSNFVA YEDPINHVN 
SIIVDIHPI EIDFNELEL EEVIGVGGF GKVYRGVWR 
GHEVAVKAA RQDPDADIS VTLENVVKE AKLFCLLKH 
ENIVSLEGV CLQEPNLCL VLEYCRGGS LNRVLAGRK 
IRPDVLVDW AIQIARGMD YLHCGAPIS LIHRDLKSS 
NVLLREAIE NDDLLSKTL KITDFGLAR EVYKTTRMS 
QAGTYAWMA PEVIKNSTF SRASDVWSY GVVLWELLT 
GETPYKGID TLAVAYGVA VNKLTLPIP STCPQPWRE 
LMEKCWKSD PHLRPSFEQ ILFDLELIF NSSFTQTPH 
ESFHIMQDD WRQEIEEVL LELRRKEKE LRCREEDLN 
RAQMKQRMH EEQLRQKEQ ELQAREIDL LERELHFMI 
KQQTPTPIK RKGKFKRSR LKLLKKEPG SNISLPSDF 
RHTITVQHT LDHKAVISP HSPPGSPAI TRLRAIALP 
ADGVKGKTW GPSTCHQRE RGQITMLRP APNRSAIWS 
KSAPNLDKT RTPVLSRQP HDIGNTSPI DSNKSSGLQ 
MLISKMSAL TTLSEFKRF GSTLRNNSF PTNNASLMQ 
KPGLGQNER LSRSIGNIT ESHYDTVFS TDSFVIARG 
VSTSVHFTK EYADVNDNH DDTERLLK
    CDS sequence
    • >TC007298 RA
      atgcctccg ttggtggag cactaccac ttcctgacg 
tccatggaa ccgccccgc aatggagac atggtcagc 
tcggttcat gtgggcgag cgaacgcac cggaccgcc 
aaccccatc acgccagcc ctgtggacg gccctgtac 
gactacgac gcgcagggc gaggacgag ctctccctg 
cgcaagggc cagatcgtg gaggtcctc tccgaggac 
gcgaagatc tccggcgac gagggctgg tggacgggg 
aaaatcggc gacaaggtc ggcatcttc ccctcgaac 
ttcgtcgcc tacgaggac cccatcaac cacgtcaac 
tccatcatc gtggacatc caccccatc gagatcgac 
ttcaacgag ctggagctc gaggaggtg atcggcgtc 
ggcgggttc gggaaggtg taccgaggc gtgtggaga 
ggacacgag gtggcggtg aaggccgcc agacaagat 
cccgacgcc gatatttcg gtcacgttg gagaacgtc 
gtcaaagaa gcgaaactt ttctgtttg ttgaaacat 
gaaaatatt gtgtcgttg gaaggtgtt tgtttacaa 
gaaccgaat ttgtgtcta gtgcttgaa tattgtcga 
ggtggttcg ctaaataga gtgttggcg gggagaaag 
attaggccg gatgttttg gtcgattgg gctatacaa 
atagcgcgc gggatggac tatttgcac tgtggggca 
cccatttca ctcatacat cgggacctg aaaagctcg 
aacgtcctc ctccgggaa gcgatcgaa aacgacgac 
ctcctgtcg aaaaccctc aagataacg gactttggt 
ttagctcgc gaagtgtac aaaacaacg cggatgtcg 
caagcgggg acctacgct tggatggcc cccgaagtc 
atcaaaaac tccacgttt tcgagagca agcgatgtt 
tggagttac ggagttgtc ttgtgggag ttgttaacg 
ggcgaaacg ccgtacaaa ggtatcgac accctggct 
gtggcttac ggcgttgcc gtcaacaaa ctcacctta 
cccataccc agcacgtgt ccgcaacct tggagggaa 
ctcatggag aaatgttgg aagtccgat ccgcacctt 
cggcccagt ttcgagcaa atccttttc gatctagaa 
ctgattttc aattcgtcg ttcactcaa actccgcac 
gaaagtttt cacataatg caagatgac tggcgtcag 
gagatcgaa gaggtcctc ttggagctt cgcaggaaa 
gagaaggag ttgcgttgc agggaagag gatctaaat 
agggcgcaa atgaagcaa aggatgcac gaggaacag 
ctgcggcag aaggaacag gaactgcaa gcgagagaa 
atcgatctt ttagaaagg gaattgcac tttatgatt 
aaacaacaa acaccaact ccgattaaa cggaaaggg 
aaattcaaa cgatcgagg ctgaaactg ctgaaaaaa 
gaacccggg tcgaatatt agcctacca tcggatttt 
cgccacacc atcacagta caacacaca ctcgaccac 
aaggctgtc atttcacca catagtcca ccgggatcc 
ccggctata acccgatta cgggcgata gcactacca 
gccgacggt gtaaagggg aaaacgtgg ggccctagc 
acgtgccac cagcgcgaa cggggccaa ataacgatg 
ctcagacca gcccccaac aggagcgcg atctggtcg 
aaaagcgcc cccaatctg gacaagacc cggacgccg 
gtgttgagc cgacagccc cacgacata ggtaacacc 
tcccccatt gacagtaac aaaagctca ggtctgcag 
atgttaatt agtaaaatg tcggctcta actactctt 
tctgaattt aaacggttc ggaagcact ctacgtaac 
aatagtttt cccacaaat aacgcttcg ctaatgcaa 
aaacccggc ttgggacag aacgaacgg ttgagcagg 
tcgatagga aatatcacc gaatcgcac tatgatact 
gtgtttagc actgatagt ttcgttatt gcgagaggg 
gtcagcact tcggtgcat ttcacgaaa gaatacgcc 
gatgttaac gacaatcac gatgatacc gaacgctta 
ctcaaataa
    mRNA sequence
    • >TC007298 RA
      agtttgcaa aaaatgtgt ttggtcaat attaaagcg 
ccaaaaaaa agcaaaaaa atagcaaaa agatagtgg 
gtatgcata tgcagccaa caatactgt agtgttttt 
gaggtaacc accgtgggg tgtcgctac atgcccaat 
ctgtcactc atcggggcg ttgaacctg tttcctcag 
tcggtaaat tcttaaatt ttgaagtat ccaatgcag 
caggaggtg gttaagaaa aaaatatgg ccgcagaag 
tgagttgtg gtgacagtc agtgaaaga aaattaaac 
ccaaaacga tgtaaataa aaatgaaag tgttttgtg 
ttgggctaa acatcggag tagatcaat ggggacgtg 
tttgagtgt tggtgacta tgtgaaaaa gtgggtgct 
gtttcggat gtatgttgt gtttggcga ccacctggc 
tctgtctgt gctgtcaac cgggcctta tcattcaaa 
ataagatgg gcattgttt attttactc ggatttatc 
aaaggagtg tcacgttga attgctgtg tttgttaga 
tttgacgat ctcgacgga tgcctccgt tggtggagc 
actaccact tcctgacgt ccatggaac cgccccgca 
atggagaca tggtcagct cggttcatg tgggcgagc 
gaacgcacc ggaccgcca accccatca cgccagccc 
tgtggacgg ccctgtacg actacgacg cgcagggcg 
aggacgagc tctccctgc gcaagggcc agatcgtgg 
aggtcctct ccgaggacg cgaagatct ccggcgacg 
agggctggt ggacgggga aaatcggcg acaaggtcg 
gcatcttcc cctcgaact tcgtcgcct acgaggacc 
ccatcaacc acgtcaact ccatcatcg tggacatcc 
accccatcg agatcgact tcaacgagc tggagctcg 
aggaggtga tcggcgtcg gcgggttcg ggaaggtgt 
accgaggcg tgtggagag gacacgagg tggcggtga 
aggccgcca gacaagatc ccgacgccg atatttcgg 
tcacgttgg agaacgtcg tcaaagaag cgaaacttt 
tctgtttgt tgaaacatg aaaatattg tgtcgttgg 
aaggtgttt gtttacaag aaccgaatt tgtgtctag 
tgcttgaat attgtcgag gtggttcgc taaatagag 
tgttggcgg ggagaaaga ttaggccgg atgttttgg 
tcgattggg ctatacaaa tagcgcgcg ggatggact 
atttgcact gtggggcac ccatttcac tcatacatc 
gggacctga aaagctcga acgtcctcc tccgggaag 
cgatcgaaa acgacgacc tcctgtcga aaaccctca 
agataacgg actttggtt tagctcgcg aagtgtaca 
aaacaacgc ggatgtcgc aagcgggga cctacgctt 
ggatggccc ccgaagtca tcaaaaact ccacgtttt 
cgagagcaa gcgatgttt ggagttacg gagttgtct 
tgtgggagt tgttaacgg gcgaaacgc cgtacaaag 
gtatcgaca ccctggctg tggcttacg gcgttgccg 
tcaacaaac tcaccttac ccataccca gcacgtgtc 
cgcaacctt ggagggaac tcatggaga aatgttgga 
agtccgatc cgcaccttc ggcccagtt tcgagcaaa 
tccttttcg atctagaac tgattttca attcgtcgt 
tcactcaaa ctccgcacg aaagttttc acataatgc 
aagatgact ggcgtcagg agatcgaag aggtcctct 
tggagcttc gcaggaaag agaaggagt tgcgttgca 
gggaagagg atctaaata gggcgcaaa tgaagcaaa 
ggatgcacg aggaacagc tgcggcaga aggaacagg 
aactgcaag cgagagaaa tcgatcttt tagaaaggg 
aattgcact ttatgatta aacaacaaa caccaactc 
cgattaaac ggaaaggga aattcaaac gatcgaggc 
tgaaactgc tgaaaaaag aacccgggt cgaatatta 
gcctaccat cggattttc gccacacca tcacagtac 
aacacacac tcgaccaca aggctgtca tttcaccac 
atagtccac cgggatccc cggctataa cccgattac 
gggcgatag cactaccag ccgacggtg taaagggga 
aaacgtggg gccctagca cgtgccacc agcgcgaac 
ggggccaaa taacgatgc tcagaccag cccccaaca 
ggagcgcga tctggtcga aaagcgccc ccaatctgg 
acaagaccc ggacgccgg tgttgagcc gacagcccc 
acgacatag gtaacacct cccccattg acagtaaca 
aaagctcag gtctgcaga tgttaatta gtaaaatgt 
cggctctaa ctactcttt ctgaattta aacggttcg 
gaagcactc tacgtaaca atagttttc ccacaaata 
acgcttcgc taatgcaaa aacccggct tgggacaga 
acgaacggt tgagcaggt cgataggaa atatcaccg 
aatcgcact atgatactg tgtttagca ctgatagtt 
tcgttattg cgagagggg tcagcactt cggtgcatt 
tcacgaaag aatacgccg atgttaacg acaatcacg 
atgataccg aacgcttac tcaaataat ttgcgcgct 
gtgaaattc tcttgttct aatcgcgac ttttcgtta 
ggcgtttcc gaggaaaac ctcccgaat gttctatgt 
aagtcagtc ccctccccc caactacgt atacttttt 
tagttttaa gcacagtag catgtcttt gatttgacc 
atattttaa ttgatttta tttcagcct tatcttaag 
tagtcttgt ttttatatt attattata ttttgtgta 
gtgtatttt agtctgtgc tgtttttta atttaattg 
atggctttt gaacacgtt ttgcgattt ttgtatgtg 
tttgaaacg tgttcaaac tgcttctct catggtgat 
ggttctatt ttatgtaat ttgattttc aataaacat 
tgtaataat gt
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