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Gene information
General information
TC007298 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6395200..6420617
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007298
    iBeetle screen iB_04308

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    abdominal segment 8 ventral medianspotted - Number of animals showing the phenotype: 3

    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007298 RA
      MPPLVEHYH FLTSMEPPR NGDMVSSVH VGERTHRTA 
      NPITPALWT ALYDYDAQG EDELSLRKG QIVEVLSED 
      AKISGDEGW WTGKIGDKV GIFPSNFVA YEDPINHVN 
      SIIVDIHPI EIDFNELEL EEVIGVGGF GKVYRGVWR 
      GHEVAVKAA RQDPDADIS VTLENVVKE AKLFCLLKH 
      ENIVSLEGV CLQEPNLCL VLEYCRGGS LNRVLAGRK 
      IRPDVLVDW AIQIARGMD YLHCGAPIS LIHRDLKSS 
      NVLLREAIE NDDLLSKTL KITDFGLAR EVYKTTRMS 
      QAGTYAWMA PEVIKNSTF SRASDVWSY GVVLWELLT 
      GETPYKGID TLAVAYGVA VNKLTLPIP STCPQPWRE 
      LMEKCWKSD PHLRPSFEQ ILFDLELIF NSSFTQTPH 
      ESFHIMQDD WRQEIEEVL LELRRKEKE LRCREEDLN 
      RAQMKQRMH EEQLRQKEQ ELQAREIDL LERELHFMI 
      KQQTPTPIK RKGKFKRSR LKLLKKEPG SNISLPSDF 
      RHTITVQHT LDHKAVISP HSPPGSPAI TRLRAIALP 
      ADGVKGKTW GPSTCHQRE RGQITMLRP APNRSAIWS 
      KSAPNLDKT RTPVLSRQP HDIGNTSPI DSNKSSGLQ 
      MLISKMSAL TTLSEFKRF GSTLRNNSF PTNNASLMQ 
      KPGLGQNER LSRSIGNIT ESHYDTVFS TDSFVIARG 
      VSTSVHFTK EYADVNDNH DDTERLLK
    CDS sequence
    • >TC007298 RA
      atgcctccg ttggtggag cactaccac ttcctgacg 
      tccatggaa ccgccccgc aatggagac atggtcagc 
      tcggttcat gtgggcgag cgaacgcac cggaccgcc 
      aaccccatc acgccagcc ctgtggacg gccctgtac 
      gactacgac gcgcagggc gaggacgag ctctccctg 
      cgcaagggc cagatcgtg gaggtcctc tccgaggac 
      gcgaagatc tccggcgac gagggctgg tggacgggg 
      aaaatcggc gacaaggtc ggcatcttc ccctcgaac 
      ttcgtcgcc tacgaggac cccatcaac cacgtcaac 
      tccatcatc gtggacatc caccccatc gagatcgac 
      ttcaacgag ctggagctc gaggaggtg atcggcgtc 
      ggcgggttc gggaaggtg taccgaggc gtgtggaga 
      ggacacgag gtggcggtg aaggccgcc agacaagat 
      cccgacgcc gatatttcg gtcacgttg gagaacgtc 
      gtcaaagaa gcgaaactt ttctgtttg ttgaaacat 
      gaaaatatt gtgtcgttg gaaggtgtt tgtttacaa 
      gaaccgaat ttgtgtcta gtgcttgaa tattgtcga 
      ggtggttcg ctaaataga gtgttggcg gggagaaag 
      attaggccg gatgttttg gtcgattgg gctatacaa 
      atagcgcgc gggatggac tatttgcac tgtggggca 
      cccatttca ctcatacat cgggacctg aaaagctcg 
      aacgtcctc ctccgggaa gcgatcgaa aacgacgac 
      ctcctgtcg aaaaccctc aagataacg gactttggt 
      ttagctcgc gaagtgtac aaaacaacg cggatgtcg 
      caagcgggg acctacgct tggatggcc cccgaagtc 
      atcaaaaac tccacgttt tcgagagca agcgatgtt 
      tggagttac ggagttgtc ttgtgggag ttgttaacg 
      ggcgaaacg ccgtacaaa ggtatcgac accctggct 
      gtggcttac ggcgttgcc gtcaacaaa ctcacctta 
      cccataccc agcacgtgt ccgcaacct tggagggaa 
      ctcatggag aaatgttgg aagtccgat ccgcacctt 
      cggcccagt ttcgagcaa atccttttc gatctagaa 
      ctgattttc aattcgtcg ttcactcaa actccgcac 
      gaaagtttt cacataatg caagatgac tggcgtcag 
      gagatcgaa gaggtcctc ttggagctt cgcaggaaa 
      gagaaggag ttgcgttgc agggaagag gatctaaat 
      agggcgcaa atgaagcaa aggatgcac gaggaacag 
      ctgcggcag aaggaacag gaactgcaa gcgagagaa 
      atcgatctt ttagaaagg gaattgcac tttatgatt 
      aaacaacaa acaccaact ccgattaaa cggaaaggg 
      aaattcaaa cgatcgagg ctgaaactg ctgaaaaaa 
      gaacccggg tcgaatatt agcctacca tcggatttt 
      cgccacacc atcacagta caacacaca ctcgaccac 
      aaggctgtc atttcacca catagtcca ccgggatcc 
      ccggctata acccgatta cgggcgata gcactacca 
      gccgacggt gtaaagggg aaaacgtgg ggccctagc 
      acgtgccac cagcgcgaa cggggccaa ataacgatg 
      ctcagacca gcccccaac aggagcgcg atctggtcg 
      aaaagcgcc cccaatctg gacaagacc cggacgccg 
      gtgttgagc cgacagccc cacgacata ggtaacacc 
      tcccccatt gacagtaac aaaagctca ggtctgcag 
      atgttaatt agtaaaatg tcggctcta actactctt 
      tctgaattt aaacggttc ggaagcact ctacgtaac 
      aatagtttt cccacaaat aacgcttcg ctaatgcaa 
      aaacccggc ttgggacag aacgaacgg ttgagcagg 
      tcgatagga aatatcacc gaatcgcac tatgatact 
      gtgtttagc actgatagt ttcgttatt gcgagaggg 
      gtcagcact tcggtgcat ttcacgaaa gaatacgcc 
      gatgttaac gacaatcac gatgatacc gaacgctta 
      ctcaaataa
    mRNA sequence
    • >TC007298 RA
      agtttgcaa aaaatgtgt ttggtcaat attaaagcg 
      ccaaaaaaa agcaaaaaa atagcaaaa agatagtgg 
      gtatgcata tgcagccaa caatactgt agtgttttt 
      gaggtaacc accgtgggg tgtcgctac atgcccaat 
      ctgtcactc atcggggcg ttgaacctg tttcctcag 
      tcggtaaat tcttaaatt ttgaagtat ccaatgcag 
      caggaggtg gttaagaaa aaaatatgg ccgcagaag 
      tgagttgtg gtgacagtc agtgaaaga aaattaaac 
      ccaaaacga tgtaaataa aaatgaaag tgttttgtg 
      ttgggctaa acatcggag tagatcaat ggggacgtg 
      tttgagtgt tggtgacta tgtgaaaaa gtgggtgct 
      gtttcggat gtatgttgt gtttggcga ccacctggc 
      tctgtctgt gctgtcaac cgggcctta tcattcaaa 
      ataagatgg gcattgttt attttactc ggatttatc 
      aaaggagtg tcacgttga attgctgtg tttgttaga 
      tttgacgat ctcgacgga tgcctccgt tggtggagc 
      actaccact tcctgacgt ccatggaac cgccccgca 
      atggagaca tggtcagct cggttcatg tgggcgagc 
      gaacgcacc ggaccgcca accccatca cgccagccc 
      tgtggacgg ccctgtacg actacgacg cgcagggcg 
      aggacgagc tctccctgc gcaagggcc agatcgtgg 
      aggtcctct ccgaggacg cgaagatct ccggcgacg 
      agggctggt ggacgggga aaatcggcg acaaggtcg 
      gcatcttcc cctcgaact tcgtcgcct acgaggacc 
      ccatcaacc acgtcaact ccatcatcg tggacatcc 
      accccatcg agatcgact tcaacgagc tggagctcg 
      aggaggtga tcggcgtcg gcgggttcg ggaaggtgt 
      accgaggcg tgtggagag gacacgagg tggcggtga 
      aggccgcca gacaagatc ccgacgccg atatttcgg 
      tcacgttgg agaacgtcg tcaaagaag cgaaacttt 
      tctgtttgt tgaaacatg aaaatattg tgtcgttgg 
      aaggtgttt gtttacaag aaccgaatt tgtgtctag 
      tgcttgaat attgtcgag gtggttcgc taaatagag 
      tgttggcgg ggagaaaga ttaggccgg atgttttgg 
      tcgattggg ctatacaaa tagcgcgcg ggatggact 
      atttgcact gtggggcac ccatttcac tcatacatc 
      gggacctga aaagctcga acgtcctcc tccgggaag 
      cgatcgaaa acgacgacc tcctgtcga aaaccctca 
      agataacgg actttggtt tagctcgcg aagtgtaca 
      aaacaacgc ggatgtcgc aagcgggga cctacgctt 
      ggatggccc ccgaagtca tcaaaaact ccacgtttt 
      cgagagcaa gcgatgttt ggagttacg gagttgtct 
      tgtgggagt tgttaacgg gcgaaacgc cgtacaaag 
      gtatcgaca ccctggctg tggcttacg gcgttgccg 
      tcaacaaac tcaccttac ccataccca gcacgtgtc 
      cgcaacctt ggagggaac tcatggaga aatgttgga 
      agtccgatc cgcaccttc ggcccagtt tcgagcaaa 
      tccttttcg atctagaac tgattttca attcgtcgt 
      tcactcaaa ctccgcacg aaagttttc acataatgc 
      aagatgact ggcgtcagg agatcgaag aggtcctct 
      tggagcttc gcaggaaag agaaggagt tgcgttgca 
      gggaagagg atctaaata gggcgcaaa tgaagcaaa 
      ggatgcacg aggaacagc tgcggcaga aggaacagg 
      aactgcaag cgagagaaa tcgatcttt tagaaaggg 
      aattgcact ttatgatta aacaacaaa caccaactc 
      cgattaaac ggaaaggga aattcaaac gatcgaggc 
      tgaaactgc tgaaaaaag aacccgggt cgaatatta 
      gcctaccat cggattttc gccacacca tcacagtac 
      aacacacac tcgaccaca aggctgtca tttcaccac 
      atagtccac cgggatccc cggctataa cccgattac 
      gggcgatag cactaccag ccgacggtg taaagggga 
      aaacgtggg gccctagca cgtgccacc agcgcgaac 
      ggggccaaa taacgatgc tcagaccag cccccaaca 
      ggagcgcga tctggtcga aaagcgccc ccaatctgg 
      acaagaccc ggacgccgg tgttgagcc gacagcccc 
      acgacatag gtaacacct cccccattg acagtaaca 
      aaagctcag gtctgcaga tgttaatta gtaaaatgt 
      cggctctaa ctactcttt ctgaattta aacggttcg 
      gaagcactc tacgtaaca atagttttc ccacaaata 
      acgcttcgc taatgcaaa aacccggct tgggacaga 
      acgaacggt tgagcaggt cgataggaa atatcaccg 
      aatcgcact atgatactg tgtttagca ctgatagtt 
      tcgttattg cgagagggg tcagcactt cggtgcatt 
      tcacgaaag aatacgccg atgttaacg acaatcacg 
      atgataccg aacgcttac tcaaataat ttgcgcgct 
      gtgaaattc tcttgttct aatcgcgac ttttcgtta 
      ggcgtttcc gaggaaaac ctcccgaat gttctatgt 
      aagtcagtc ccctccccc caactacgt atacttttt 
      tagttttaa gcacagtag catgtcttt gatttgacc 
      atattttaa ttgatttta tttcagcct tatcttaag 
      tagtcttgt ttttatatt attattata ttttgtgta 
      gtgtatttt agtctgtgc tgtttttta atttaattg 
      atggctttt gaacacgtt ttgcgattt ttgtatgtg 
      tttgaaacg tgttcaaac tgcttctct catggtgat 
      ggttctatt ttatgtaat ttgattttc aataaacat 
      tgtaataat gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY