Status
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Gene information
General information
TC031107
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007291,TC007289,TC007290
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6493880..6497637
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031107
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04306

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: sometimes inside out
    larva anterior mostlynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031107 RA
      MTRQNTIPF QEDYYALIP LWDMCNHTN GTISTAYNP 
      VLDRSECLA VKNFKAGEQ LFIFYGSRS NADLFVHNG 
      FVFENNDYD VYWIRLGIS KSDPLQQKR GHLLGKLSI 
      ASTCDFSIR KGASPIDGQ LLAFLRVFN MNEEQLDHW 
      INSDKSADL GHIDCALDT ALETKSWRF LHARLKLLL 
      STYKTTLDE DEKLLAEAQ ATPNRLLAI KMRATEKRI 
      IRETLEYVE HASGEESLQ PFLAEDERL LLRFRKKEL 
      LFKILVIGD FGVGKTSII RRYADGTFS SSYKITIGA 
      DFALKSLTW DKNTQVNVQ LWDIAGHER FGYLTGVYY 
      RYAVAAAIV FDLTRPETF KSVEKWLLD LRKKVHLPG 
      GVPVPVVIL ANKGDITIA TLPQEIVHF CEKYNILAW 
      FITSAKDNV HIDEAMLTL INAAVENHE NLQFPAAVD 
      NDVVHLANE DTQFCQNNQ THCCQR
    CDS sequence
    • >TC031107 RA
      atgacacgt cagaacacc atccctttc caggaggat 
      tactacgct cttatacca ttatgggac atgtgcaac 
      cacacaaac ggaactata tcgacagct tacaacccg 
      gtgctcgat cgcagcgag tgtttagcg gtaaaaaac 
      ttcaaagcg ggagaacaa ttattcatc ttctacgga 
      agcagaagt aacgccgat ctttttgtt cacaacgga 
      tttgtgttc gaaaacaac gactatgac gtgtattgg 
      atccgtttg gggatcagc aagtcggac cctttgcag 
      cagaagagg ggtcatttg ctgggtaaa ttgtcgatt 
      gccagcact tgcgacttt tctattaga aaaggtgcg 
      agtccgatc gacggccag ctgttggcc ttcttgcgc 
      gttttcaac atgaacgag gaacaactc gaccattgg 
      ataaatagc gacaaaagc gccgatttg gggcacatt 
      gactgcgct ctagacacg gctttggag acgaaatcg 
      tggcggttc ctccacgcc agactcaag ctgctttta 
      tcaacgtac aaaacaaca ttggacgaa gacgagaaa 
      cttctagca gaagcgcaa gcaacacct aatcggctc 
      ctggcgata aaaatgagg gcgacggag aaacgcatc 
      atcagagaa acactcgaa tatgtggaa catgcctct 
      ggggaagag tcgctccag ccttttttg gccgaagat 
      gagcgcctt ttactcaga tttcgcaaa aaagaattg 
      cttttcaaa attctagtc attggagat ttcggagtt 
      gggaaaact tcgataatt agacgatac gctgatggt 
      acattttcg tcttcgtat aaaattaca attggcgcc 
      gacttcgca cttaaatct ttaacgtgg gacaaaaat 
      actcaagtt aatgtgcaa ttatgggac attgcgggg 
      cacgaacga tttggttac ctcacaggt gtttactac 
      agatatgca gttgccgcc gctattgtt tttgatctg 
      acgagacct gagactttt aaatcggta gaaaagtgg 
      ttgctcgat ctgcgcaaa aaagtgcac ctaccggga 
      ggtgttccc gtacctgtt gtgattcta gcaaataag 
      ggtgatatt acgattgca accctgcct caagaaata 
      gtgcatttc tgcgagaaa tacaacata ttagcgtgg 
      tttatcact tcagcgaag gataatgtc cacatagat 
      gaggcaatg ttgactctg ataaatgca gcagtggaa 
      aatcacgaa aatttgcaa tttcccgct gccgtcgac 
      aatgacgtg gtccattta gcaaatgag gatactcag 
      ttttgtcaa aacaatcaa acacactgt tgtcaaaga 
      tga
    mRNA sequence
    • >TC031107 RA
      tacgtgacc cgagattaa aaaagaaaa gctgggcgg 
      tttctaccg tgatgacac gtcagaaca ccatccctt 
      tccaggagg attactacg ctcttatac cattatggg 
      acatgtgca accacacaa acggaacta tatcgacag 
      cttacaacc cggtgctcg atcgcagcg agtgtttag 
      cggtaaaaa acttcaaag cgggagaac aattattca 
      tcttctacg gaagcagaa gtaacgccg atctttttg 
      ttcacaacg gatttgtgt tcgaaaaca acgactatg 
      acgtgtatt ggatccgtt tggggatca gcaagtcgg 
      accctttgc agcagaaga ggggtcatt tgctgggta 
      aattgtcga ttgccagca cttgcgact tttctatta 
      gaaaaggtg cgagtccga tcgacggcc agctgttgg 
      ccttcttgc gcgttttca acatgaacg aggaacaac 
      tcgaccatt ggataaata gcgacaaaa gcgccgatt 
      tggggcaca ttgactgcg ctctagaca cggctttgg 
      agacgaaat cgtggcggt tcctccacg ccagactca 
      agctgcttt tatcaacgt acaaaacaa cattggacg 
      aagacgaga aacttctag cagaagcgc aagcaacac 
      ctaatcggc tcctggcga taaaaatga gggcgacgg 
      agaaacgca tcatcagag aaacactcg aatatgtgg 
      aacatgcct ctggggaag agtcgctcc agccttttt 
      tggccgaag atgagcgcc ttttactca gatttcgca 
      aaaaagaat tgcttttca aaattctag tcattggag 
      atttcggag ttgggaaaa cttcgataa ttagacgat 
      acgctgatg gtacatttt cgtcttcgt ataaaatta 
      caattggcg ccgacttcg cacttaaat ctttaacgt 
      gggacaaaa atactcaag ttaatgtgc aattatggg 
      acattgcgg ggcacgaac gatttggtt acctcacag 
      gtgtttact acagatatg cagttgccg ccgctattg 
      tttttgatc tgacgagac ctgagactt ttaaatcgg 
      tagaaaagt ggttgctcg atctgcgca aaaaagtgc 
      acctaccgg gaggtgttc ccgtacctg ttgtgattc 
      tagcaaata agggtgata ttacgattg caaccctgc 
      ctcaagaaa tagtgcatt tctgcgaga aatacaaca 
      tattagcgt ggtttatca cttcagcga aggataatg 
      tccacatag atgaggcaa tgttgactc tgataaatg 
      cagcagtgg aaaatcacg aaaatttgc aatttcccg 
      ctgccgtcg acaatgacg tggtccatt tagcaaatg 
      aggatactc agttttgtc aaaacaatc aaacacact 
      gttgtcaaa gatgaggct atattttgt aacaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY