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Gene information
General information
TC007276 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6621432..6625774
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007276
    iBeetle screen iB_04304

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva bristle random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva bristle number increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    gena triplet gena triplet dorsal number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax bristle random number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007276 RA
      MATIGAEVG QKMRSAIKA KLLELNCYV DDELPDYVM 
      VMVANKRTK SQMKDDLQL FLSNKTATF VDWLHIVLK 
      KLKEVTVTN PDVYKKPPK RKSDELPNV NVKKEKKEK 
      KSGKKETKT EPEQSTESV FKSLTDDLP ISANRLSEQ 
      RKIIIMKEN KSNNSRKVM QDNFDIPLL SEVNMSTEE 
      ELEDLENKI KSVKSRLGL LVESDEEED CIKLKPEPD 
      ELLPTENEN KGESKVEST TTAAPQPPK RTEHSRITF 
      NEEHETRRK SILDRLGKR RSGDNEEAA SSKRNRIDL 
      SDFRKEDSS KTRSDRREK RFRRDDKSK EGVLSRVGV 
      MSKVSVPVK APEEPEEPF KNREVPSVV KIKPRVIPP 
      DAPQANKNL LLKAVAEAQ KSIAQAPKV GNNLKPDAL 
      YTKKYKEKR KDEPSSGKK LPEVEKNKI KKFLAVSDD 
      DSKRDETEE YIPTPIKRV QESTTKYIP TSKTNDSDD 
      SSVENGKVK QQFIVTLDG IERTKYKDL ANEEPKPSV 
      KSRLDKKKS PSPIIFDKV ENTIKGKPN IPDKLPLIH 
      ASPAVKNKE RCKYWPSCR QGDKCEFVH PTTTCEKFP 
      HCKFGDKCL FLHPTCKFG SSCTRRGCV YSHAAKGTG 
      PSPVKLAGP VQTCKFFPN CTNVNCAFY HPKPCKFGK 
      YCKNQADCT FSHTFVPNK SSLTWRSK
    CDS sequence
    • >TC007276 RA
      atggcaaca ataggtgcg gaagttggc caaaaaatg 
      cgatccgca atcaaagcc aaactttta gaattgaac 
      tgttacgta gatgatgaa cttccagac tatgttatg 
      gtgatggtg gcaaataag cggacaaaa tcgcagatg 
      aaagacgat ttgcaactg tttctttcc aacaaaact 
      gcaactttt gtcgattgg cttcatatc gtattgaaa 
      aaactgaaa gaagtaaca gttacaaac ccagatgtg 
      tacaagaaa ccgccaaaa cgaaagtct gacgaatta 
      ccaaacgtt aacgttaaa aaagagaag aaggaaaag 
      aagtctggg aaaaaagaa acgaaaact gaaccagaa 
      caatcaaca gaatctgtc ttcaaatca ctgactgac 
      gatttaccc ataagtgca aacagactc tccgagcaa 
      cgaaaaatc ataataatg aaagaaaac aaatctaac 
      aacagccgg aaagtgatg caagacaac ttcgatata 
      cccctactt tcagaagtc aatatgtcc acagaagaa 
      gaactcgaa gacttggaa aacaagata aaaagcgtg 
      aagagcagg ctgggtctg ctggtcgag agcgatgaa 
      gaagaagat tgtattaaa cttaaacct gagccagac 
      gagttattg cccacagaa aatgaaaat aaaggagag 
      agtaaagtt gaatcaaca accactgcc gccccacaa 
      ccacccaaa aggactgaa cattctagg attacattt 
      aatgaggaa catgaaact agacgaaaa tctatcctg 
      gatagatta gggaagagg cgttccggt gataacgag 
      gaagccgct tcgtcaaaa agaaaccgg attgatttg 
      tcggatttt cgcaaagag gatagttca aaaactaga 
      agtgataga agagaaaaa cggtttaga agagatgac 
      aaatcaaaa gagggtgtt ttgagccga gttggggtg 
      atgtcaaaa gtatcggtt cctgtgaaa gccccggaa 
      gaaccagaa gaacccttt aaaaatcgg gaagttcca 
      agtgtggtt aaaattaaa ccaagggtt attccgcct 
      gacgcacct caagctaat aaaaatttg cttttgaaa 
      gctgtagcc gaagcccag aagagcata gcacaggca 
      ccaaaggtt ggaaataat ttaaagcct gatgctttg 
      tatacgaaa aaatacaaa gaaaaacgt aaggatgag 
      ccgtcatct ggcaaaaaa ttgccagaa gttgagaaa 
      aataaaata aaaaaattc ctcgccgta tctgatgat 
      gatagcaag agggacgaa acggaagaa tacatcccg 
      actccgatt aaaagagtg caggaatcc actactaaa 
      tacatccct actagtaaa acaaacgat tcagatgac 
      agttccgtt gaaaatggt aaagttaag caacaattc 
      atagtcact ttggatggt attgaaaga accaaatac 
      aaagatttg gctaacgaa gagccaaaa ccttcagtc 
      aagtcgcga cttgacaaa aagaaatca ccctcccct 
      attattttc gacaaagtt gagaacaca atcaagggg 
      aaaccaaat attcccgac aaactaccc ctaattcat 
      gcctcgccc gctgtcaaa aataaagaa aggtgtaaa 
      tattggccg tcctgtcga cagggtgat aaatgtgaa 
      tttgtccac cctactacc acatgtgaa aaattccca 
      cattgtaag ttcggtgat aagtgttta tttttgcat 
      ccaacttgc aaatttgga agttcttgt accagaaga 
      ggctgtgtt tatagtcac gcagccaaa ggaacaggc 
      ccaagtcct gtaaagttg gcgggacct gtgcaaact 
      tgtaaattt ttccccaac tgtacaaac gtcaattgt 
      gctttctac caccctaaa ccttgtaaa tttggaaaa 
      tattgcaaa aatcaagcg gattgtacc ttctctcac 
      acttttgtt cctaacaag agtagtctc acttggaga 
      tcaaagtag
    mRNA sequence
    • >TC007276 RA
      atgattggc taatgcacc ttggcgtaa tttaacagt 
      gatttgtca aaatggcaa caataggtg cggaagttg 
      gccaaaaaa tgcgatccg caatcaaag ccaaacttt 
      tagaattga actgttacg tagatgatg aacttccag 
      actatgtta tggtgatgg tggcaaata agcggacaa 
      aatcgcaga tgaaagacg atttgcaac tgtttcttt 
      ccaacaaaa ctgcaactt ttgtcgatt ggcttcata 
      tcgtattga aaaaactga aagaagtaa cagttacaa 
      acccagatg tgtacaaga aaccgccaa aacgaaagt 
      ctgacgaat taccaaacg ttaacgtta aaaaagaga 
      agaaggaaa agaagtctg ggaaaaaag aaacgaaaa 
      ctgaaccag aacaatcaa cagaatctg tcttcaaat 
      cactgactg acgatttac ccataagtg caaacagac 
      tctccgagc aacgaaaaa tcataataa tgaaagaaa 
      acaaatcta acaacagcc ggaaagtga tgcaagaca 
      acttcgata tacccctac tttcagaag tcaatatgt 
      ccacagaag aagaactcg aagacttgg aaaacaaga 
      taaaaagcg tgaagagca ggctgggtc tgctggtcg 
      agagcgatg aagaagaag attgtatta aacttaaac 
      ctgagccag acgagttat tgcccacag aaaatgaaa 
      ataaaggag agagtaaag ttgaatcaa caaccactg 
      ccgccccac aaccaccca aaaggactg aacattcta 
      ggattacat ttaatgagg aacatgaaa ctagacgaa 
      aatctatcc tggatagat tagggaaga ggcgttccg 
      gtgataacg aggaagccg cttcgtcaa aaagaaacc 
      ggattgatt tgtcggatt ttcgcaaag aggatagtt 
      caaaaacta gaagtgata gaagagaaa aacggttta 
      gaagagatg acaaatcaa aagagggtg ttttgagcc 
      gagttgggg tgatgtcaa aagtatcgg ttcctgtga 
      aagccccgg aagaaccag aagaaccct ttaaaaatc 
      gggaagttc caagtgtgg ttaaaatta aaccaaggg 
      ttattccgc ctgacgcac ctcaagcta ataaaaatt 
      tgcttttga aagctgtag ccgaagccc agaagagca 
      tagcacagg caccaaagg ttggaaata atttaaagc 
      ctgatgctt tgtatacga aaaaataca aagaaaaac 
      gtaaggatg agccgtcat ctggcaaaa aattgccag 
      aagttgaga aaaataaaa taaaaaaat tcctcgccg 
      tatctgatg atgatagca agagggacg aaacggaag 
      aatacatcc cgactccga ttaaaagag tgcaggaat 
      ccactacta aatacatcc ctactagta aaacaaacg 
      attcagatg acagttccg ttgaaaatg gtaaagtta 
      agcaacaat tcatagtca ctttggatg gtattgaaa 
      gaaccaaat acaaagatt tggctaacg aagagccaa 
      aaccttcag tcaagtcgc gacttgaca aaaagaaat 
      caccctccc ctattattt tcgacaaag ttgagaaca 
      caatcaagg ggaaaccaa atattcccg acaaactac 
      ccctaattc atgcctcgc ccgctgtca aaaataaag 
      aaaggtgta aatattggc cgtcctgtc gacagggtg 
      ataaatgtg aatttgtcc accctacta ccacatgtg 
      aaaaattcc cacattgta agttcggtg ataagtgtt 
      tatttttgc atccaactt gcaaatttg gaagttctt 
      gtaccagaa gaggctgtg tttatagtc acgcagcca 
      aaggaacag gcccaagtc ctgtaaagt tggcgggac 
      ctgtgcaaa cttgtaaat ttttcccca actgtacaa 
      acgtcaatt gtgctttct accacccta aaccttgta 
      aatttggaa aatattgca aaaatcaag cggattgta 
      ccttctctc acacttttg ttcctaaca agagtagtc 
      tcacttgga gatcaaagt agtagctta cagttagtt 
      ataccatgc tcaggtaaa tttgtgaaa attatgttg 
      gccacgttg atgtcgcag aggactttg atcacaaat 
      ttaatttag ggcacggta taaactcaa cttgtttca 
      atattacac aagtcacac tttttctgt catgcgatt 
      atgtaacag gattgtaat aggtagagg ataaaatat 
      ttaaaaatg tactgtctg aataaataa ttgaacgag 
      tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY