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Gene information
General information
TC007276 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6621432..6625774
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007276
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04304

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     larva bristle random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva bristle number increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    gena triplet gena triplet dorsal number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax bristle random number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007276 RA
      MATIGAEVG QKMRSAIKA KLLELNCYV DDELPDYVM 
VMVANKRTK SQMKDDLQL FLSNKTATF VDWLHIVLK 
KLKEVTVTN PDVYKKPPK RKSDELPNV NVKKEKKEK 
KSGKKETKT EPEQSTESV FKSLTDDLP ISANRLSEQ 
RKIIIMKEN KSNNSRKVM QDNFDIPLL SEVNMSTEE 
ELEDLENKI KSVKSRLGL LVESDEEED CIKLKPEPD 
ELLPTENEN KGESKVEST TTAAPQPPK RTEHSRITF 
NEEHETRRK SILDRLGKR RSGDNEEAA SSKRNRIDL 
SDFRKEDSS KTRSDRREK RFRRDDKSK EGVLSRVGV 
MSKVSVPVK APEEPEEPF KNREVPSVV KIKPRVIPP 
DAPQANKNL LLKAVAEAQ KSIAQAPKV GNNLKPDAL 
YTKKYKEKR KDEPSSGKK LPEVEKNKI KKFLAVSDD 
DSKRDETEE YIPTPIKRV QESTTKYIP TSKTNDSDD 
SSVENGKVK QQFIVTLDG IERTKYKDL ANEEPKPSV 
KSRLDKKKS PSPIIFDKV ENTIKGKPN IPDKLPLIH 
ASPAVKNKE RCKYWPSCR QGDKCEFVH PTTTCEKFP 
HCKFGDKCL FLHPTCKFG SSCTRRGCV YSHAAKGTG 
PSPVKLAGP VQTCKFFPN CTNVNCAFY HPKPCKFGK 
YCKNQADCT FSHTFVPNK SSLTWRSK
    CDS sequence
    • >TC007276 RA
      atggcaaca ataggtgcg gaagttggc caaaaaatg 
cgatccgca atcaaagcc aaactttta gaattgaac 
tgttacgta gatgatgaa cttccagac tatgttatg 
gtgatggtg gcaaataag cggacaaaa tcgcagatg 
aaagacgat ttgcaactg tttctttcc aacaaaact 
gcaactttt gtcgattgg cttcatatc gtattgaaa 
aaactgaaa gaagtaaca gttacaaac ccagatgtg 
tacaagaaa ccgccaaaa cgaaagtct gacgaatta 
ccaaacgtt aacgttaaa aaagagaag aaggaaaag 
aagtctggg aaaaaagaa acgaaaact gaaccagaa 
caatcaaca gaatctgtc ttcaaatca ctgactgac 
gatttaccc ataagtgca aacagactc tccgagcaa 
cgaaaaatc ataataatg aaagaaaac aaatctaac 
aacagccgg aaagtgatg caagacaac ttcgatata 
cccctactt tcagaagtc aatatgtcc acagaagaa 
gaactcgaa gacttggaa aacaagata aaaagcgtg 
aagagcagg ctgggtctg ctggtcgag agcgatgaa 
gaagaagat tgtattaaa cttaaacct gagccagac 
gagttattg cccacagaa aatgaaaat aaaggagag 
agtaaagtt gaatcaaca accactgcc gccccacaa 
ccacccaaa aggactgaa cattctagg attacattt 
aatgaggaa catgaaact agacgaaaa tctatcctg 
gatagatta gggaagagg cgttccggt gataacgag 
gaagccgct tcgtcaaaa agaaaccgg attgatttg 
tcggatttt cgcaaagag gatagttca aaaactaga 
agtgataga agagaaaaa cggtttaga agagatgac 
aaatcaaaa gagggtgtt ttgagccga gttggggtg 
atgtcaaaa gtatcggtt cctgtgaaa gccccggaa 
gaaccagaa gaacccttt aaaaatcgg gaagttcca 
agtgtggtt aaaattaaa ccaagggtt attccgcct 
gacgcacct caagctaat aaaaatttg cttttgaaa 
gctgtagcc gaagcccag aagagcata gcacaggca 
ccaaaggtt ggaaataat ttaaagcct gatgctttg 
tatacgaaa aaatacaaa gaaaaacgt aaggatgag 
ccgtcatct ggcaaaaaa ttgccagaa gttgagaaa 
aataaaata aaaaaattc ctcgccgta tctgatgat 
gatagcaag agggacgaa acggaagaa tacatcccg 
actccgatt aaaagagtg caggaatcc actactaaa 
tacatccct actagtaaa acaaacgat tcagatgac 
agttccgtt gaaaatggt aaagttaag caacaattc 
atagtcact ttggatggt attgaaaga accaaatac 
aaagatttg gctaacgaa gagccaaaa ccttcagtc 
aagtcgcga cttgacaaa aagaaatca ccctcccct 
attattttc gacaaagtt gagaacaca atcaagggg 
aaaccaaat attcccgac aaactaccc ctaattcat 
gcctcgccc gctgtcaaa aataaagaa aggtgtaaa 
tattggccg tcctgtcga cagggtgat aaatgtgaa 
tttgtccac cctactacc acatgtgaa aaattccca 
cattgtaag ttcggtgat aagtgttta tttttgcat 
ccaacttgc aaatttgga agttcttgt accagaaga 
ggctgtgtt tatagtcac gcagccaaa ggaacaggc 
ccaagtcct gtaaagttg gcgggacct gtgcaaact 
tgtaaattt ttccccaac tgtacaaac gtcaattgt 
gctttctac caccctaaa ccttgtaaa tttggaaaa 
tattgcaaa aatcaagcg gattgtacc ttctctcac 
acttttgtt cctaacaag agtagtctc acttggaga 
tcaaagtag
    mRNA sequence
    • >TC007276 RA
      atgattggc taatgcacc ttggcgtaa tttaacagt 
gatttgtca aaatggcaa caataggtg cggaagttg 
gccaaaaaa tgcgatccg caatcaaag ccaaacttt 
tagaattga actgttacg tagatgatg aacttccag 
actatgtta tggtgatgg tggcaaata agcggacaa 
aatcgcaga tgaaagacg atttgcaac tgtttcttt 
ccaacaaaa ctgcaactt ttgtcgatt ggcttcata 
tcgtattga aaaaactga aagaagtaa cagttacaa 
acccagatg tgtacaaga aaccgccaa aacgaaagt 
ctgacgaat taccaaacg ttaacgtta aaaaagaga 
agaaggaaa agaagtctg ggaaaaaag aaacgaaaa 
ctgaaccag aacaatcaa cagaatctg tcttcaaat 
cactgactg acgatttac ccataagtg caaacagac 
tctccgagc aacgaaaaa tcataataa tgaaagaaa 
acaaatcta acaacagcc ggaaagtga tgcaagaca 
acttcgata tacccctac tttcagaag tcaatatgt 
ccacagaag aagaactcg aagacttgg aaaacaaga 
taaaaagcg tgaagagca ggctgggtc tgctggtcg 
agagcgatg aagaagaag attgtatta aacttaaac 
ctgagccag acgagttat tgcccacag aaaatgaaa 
ataaaggag agagtaaag ttgaatcaa caaccactg 
ccgccccac aaccaccca aaaggactg aacattcta 
ggattacat ttaatgagg aacatgaaa ctagacgaa 
aatctatcc tggatagat tagggaaga ggcgttccg 
gtgataacg aggaagccg cttcgtcaa aaagaaacc 
ggattgatt tgtcggatt ttcgcaaag aggatagtt 
caaaaacta gaagtgata gaagagaaa aacggttta 
gaagagatg acaaatcaa aagagggtg ttttgagcc 
gagttgggg tgatgtcaa aagtatcgg ttcctgtga 
aagccccgg aagaaccag aagaaccct ttaaaaatc 
gggaagttc caagtgtgg ttaaaatta aaccaaggg 
ttattccgc ctgacgcac ctcaagcta ataaaaatt 
tgcttttga aagctgtag ccgaagccc agaagagca 
tagcacagg caccaaagg ttggaaata atttaaagc 
ctgatgctt tgtatacga aaaaataca aagaaaaac 
gtaaggatg agccgtcat ctggcaaaa aattgccag 
aagttgaga aaaataaaa taaaaaaat tcctcgccg 
tatctgatg atgatagca agagggacg aaacggaag 
aatacatcc cgactccga ttaaaagag tgcaggaat 
ccactacta aatacatcc ctactagta aaacaaacg 
attcagatg acagttccg ttgaaaatg gtaaagtta 
agcaacaat tcatagtca ctttggatg gtattgaaa 
gaaccaaat acaaagatt tggctaacg aagagccaa 
aaccttcag tcaagtcgc gacttgaca aaaagaaat 
caccctccc ctattattt tcgacaaag ttgagaaca 
caatcaagg ggaaaccaa atattcccg acaaactac 
ccctaattc atgcctcgc ccgctgtca aaaataaag 
aaaggtgta aatattggc cgtcctgtc gacagggtg 
ataaatgtg aatttgtcc accctacta ccacatgtg 
aaaaattcc cacattgta agttcggtg ataagtgtt 
tatttttgc atccaactt gcaaatttg gaagttctt 
gtaccagaa gaggctgtg tttatagtc acgcagcca 
aaggaacag gcccaagtc ctgtaaagt tggcgggac 
ctgtgcaaa cttgtaaat ttttcccca actgtacaa 
acgtcaatt gtgctttct accacccta aaccttgta 
aatttggaa aatattgca aaaatcaag cggattgta 
ccttctctc acacttttg ttcctaaca agagtagtc 
tcacttgga gatcaaagt agtagctta cagttagtt 
ataccatgc tcaggtaaa tttgtgaaa attatgttg 
gccacgttg atgtcgcag aggactttg atcacaaat 
ttaatttag ggcacggta taaactcaa cttgtttca 
atattacac aagtcacac tttttctgt catgcgatt 
atgtaacag gattgtaat aggtagagg ataaaatat 
ttaaaaatg tactgtctg aataaataa ttgaacgag 
tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY