General information | |||
TC007276 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 6621432..6625774 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC007276 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04304 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 2
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
larva bristle random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva bristle number increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
gena triplet gena triplet dorsal number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax bristle random number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007276 RA
MATIGAEVG QKMRSAIKA KLLELNCYV DDELPDYVM VMVANKRTK SQMKDDLQL FLSNKTATF VDWLHIVLK KLKEVTVTN PDVYKKPPK RKSDELPNV NVKKEKKEK KSGKKETKT EPEQSTESV FKSLTDDLP ISANRLSEQ RKIIIMKEN KSNNSRKVM QDNFDIPLL SEVNMSTEE ELEDLENKI KSVKSRLGL LVESDEEED CIKLKPEPD ELLPTENEN KGESKVEST TTAAPQPPK RTEHSRITF NEEHETRRK SILDRLGKR RSGDNEEAA SSKRNRIDL SDFRKEDSS KTRSDRREK RFRRDDKSK EGVLSRVGV MSKVSVPVK APEEPEEPF KNREVPSVV KIKPRVIPP DAPQANKNL LLKAVAEAQ KSIAQAPKV GNNLKPDAL YTKKYKEKR KDEPSSGKK LPEVEKNKI KKFLAVSDD DSKRDETEE YIPTPIKRV QESTTKYIP TSKTNDSDD SSVENGKVK QQFIVTLDG IERTKYKDL ANEEPKPSV KSRLDKKKS PSPIIFDKV ENTIKGKPN IPDKLPLIH ASPAVKNKE RCKYWPSCR QGDKCEFVH PTTTCEKFP HCKFGDKCL FLHPTCKFG SSCTRRGCV YSHAAKGTG PSPVKLAGP VQTCKFFPN CTNVNCAFY HPKPCKFGK YCKNQADCT FSHTFVPNK SSLTWRSK
- >TC007276 RA
atggcaaca ataggtgcg gaagttggc caaaaaatg cgatccgca atcaaagcc aaactttta gaattgaac tgttacgta gatgatgaa cttccagac tatgttatg gtgatggtg gcaaataag cggacaaaa tcgcagatg aaagacgat ttgcaactg tttctttcc aacaaaact gcaactttt gtcgattgg cttcatatc gtattgaaa aaactgaaa gaagtaaca gttacaaac ccagatgtg tacaagaaa ccgccaaaa cgaaagtct gacgaatta ccaaacgtt aacgttaaa aaagagaag aaggaaaag aagtctggg aaaaaagaa acgaaaact gaaccagaa caatcaaca gaatctgtc ttcaaatca ctgactgac gatttaccc ataagtgca aacagactc tccgagcaa cgaaaaatc ataataatg aaagaaaac aaatctaac aacagccgg aaagtgatg caagacaac ttcgatata cccctactt tcagaagtc aatatgtcc acagaagaa gaactcgaa gacttggaa aacaagata aaaagcgtg aagagcagg ctgggtctg ctggtcgag agcgatgaa gaagaagat tgtattaaa cttaaacct gagccagac gagttattg cccacagaa aatgaaaat aaaggagag agtaaagtt gaatcaaca accactgcc gccccacaa ccacccaaa aggactgaa cattctagg attacattt aatgaggaa catgaaact agacgaaaa tctatcctg gatagatta gggaagagg cgttccggt gataacgag gaagccgct tcgtcaaaa agaaaccgg attgatttg tcggatttt cgcaaagag gatagttca aaaactaga agtgataga agagaaaaa cggtttaga agagatgac aaatcaaaa gagggtgtt ttgagccga gttggggtg atgtcaaaa gtatcggtt cctgtgaaa gccccggaa gaaccagaa gaacccttt aaaaatcgg gaagttcca agtgtggtt aaaattaaa ccaagggtt attccgcct gacgcacct caagctaat aaaaatttg cttttgaaa gctgtagcc gaagcccag aagagcata gcacaggca ccaaaggtt ggaaataat ttaaagcct gatgctttg tatacgaaa aaatacaaa gaaaaacgt aaggatgag ccgtcatct ggcaaaaaa ttgccagaa gttgagaaa aataaaata aaaaaattc ctcgccgta tctgatgat gatagcaag agggacgaa acggaagaa tacatcccg actccgatt aaaagagtg caggaatcc actactaaa tacatccct actagtaaa acaaacgat tcagatgac agttccgtt gaaaatggt aaagttaag caacaattc atagtcact ttggatggt attgaaaga accaaatac aaagatttg gctaacgaa gagccaaaa ccttcagtc aagtcgcga cttgacaaa aagaaatca ccctcccct attattttc gacaaagtt gagaacaca atcaagggg aaaccaaat attcccgac aaactaccc ctaattcat gcctcgccc gctgtcaaa aataaagaa aggtgtaaa tattggccg tcctgtcga cagggtgat aaatgtgaa tttgtccac cctactacc acatgtgaa aaattccca cattgtaag ttcggtgat aagtgttta tttttgcat ccaacttgc aaatttgga agttcttgt accagaaga ggctgtgtt tatagtcac gcagccaaa ggaacaggc ccaagtcct gtaaagttg gcgggacct gtgcaaact tgtaaattt ttccccaac tgtacaaac gtcaattgt gctttctac caccctaaa ccttgtaaa tttggaaaa tattgcaaa aatcaagcg gattgtacc ttctctcac acttttgtt cctaacaag agtagtctc acttggaga tcaaagtag
- >TC007276 RA
atgattggc taatgcacc ttggcgtaa tttaacagt gatttgtca aaatggcaa caataggtg cggaagttg gccaaaaaa tgcgatccg caatcaaag ccaaacttt tagaattga actgttacg tagatgatg aacttccag actatgtta tggtgatgg tggcaaata agcggacaa aatcgcaga tgaaagacg atttgcaac tgtttcttt ccaacaaaa ctgcaactt ttgtcgatt ggcttcata tcgtattga aaaaactga aagaagtaa cagttacaa acccagatg tgtacaaga aaccgccaa aacgaaagt ctgacgaat taccaaacg ttaacgtta aaaaagaga agaaggaaa agaagtctg ggaaaaaag aaacgaaaa ctgaaccag aacaatcaa cagaatctg tcttcaaat cactgactg acgatttac ccataagtg caaacagac tctccgagc aacgaaaaa tcataataa tgaaagaaa acaaatcta acaacagcc ggaaagtga tgcaagaca acttcgata tacccctac tttcagaag tcaatatgt ccacagaag aagaactcg aagacttgg aaaacaaga taaaaagcg tgaagagca ggctgggtc tgctggtcg agagcgatg aagaagaag attgtatta aacttaaac ctgagccag acgagttat tgcccacag aaaatgaaa ataaaggag agagtaaag ttgaatcaa caaccactg ccgccccac aaccaccca aaaggactg aacattcta ggattacat ttaatgagg aacatgaaa ctagacgaa aatctatcc tggatagat tagggaaga ggcgttccg gtgataacg aggaagccg cttcgtcaa aaagaaacc ggattgatt tgtcggatt ttcgcaaag aggatagtt caaaaacta gaagtgata gaagagaaa aacggttta gaagagatg acaaatcaa aagagggtg ttttgagcc gagttgggg tgatgtcaa aagtatcgg ttcctgtga aagccccgg aagaaccag aagaaccct ttaaaaatc gggaagttc caagtgtgg ttaaaatta aaccaaggg ttattccgc ctgacgcac ctcaagcta ataaaaatt tgcttttga aagctgtag ccgaagccc agaagagca tagcacagg caccaaagg ttggaaata atttaaagc ctgatgctt tgtatacga aaaaataca aagaaaaac gtaaggatg agccgtcat ctggcaaaa aattgccag aagttgaga aaaataaaa taaaaaaat tcctcgccg tatctgatg atgatagca agagggacg aaacggaag aatacatcc cgactccga ttaaaagag tgcaggaat ccactacta aatacatcc ctactagta aaacaaacg attcagatg acagttccg ttgaaaatg gtaaagtta agcaacaat tcatagtca ctttggatg gtattgaaa gaaccaaat acaaagatt tggctaacg aagagccaa aaccttcag tcaagtcgc gacttgaca aaaagaaat caccctccc ctattattt tcgacaaag ttgagaaca caatcaagg ggaaaccaa atattcccg acaaactac ccctaattc atgcctcgc ccgctgtca aaaataaag aaaggtgta aatattggc cgtcctgtc gacagggtg ataaatgtg aatttgtcc accctacta ccacatgtg aaaaattcc cacattgta agttcggtg ataagtgtt tatttttgc atccaactt gcaaatttg gaagttctt gtaccagaa gaggctgtg tttatagtc acgcagcca aaggaacag gcccaagtc ctgtaaagt tggcgggac ctgtgcaaa cttgtaaat ttttcccca actgtacaa acgtcaatt gtgctttct accacccta aaccttgta aatttggaa aatattgca aaaatcaag cggattgta ccttctctc acacttttg ttcctaaca agagtagtc tcacttgga gatcaaagt agtagctta cagttagtt ataccatgc tcaggtaaa tttgtgaaa attatgttg gccacgttg atgtcgcag aggactttg atcacaaat ttaatttag ggcacggta taaactcaa cttgtttca atattacac aagtcacac tttttctgt catgcgatt atgtaacag gattgtaat aggtagagg ataaaatat ttaaaaatg tactgtctg aataaataa ttgaacgag tt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
