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Gene information
General information
TC007264 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6689794..6699714
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007264
    iBeetle screen iB_04303

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007264 RA
      MFLVIVLLG CLIQNSLSE YNNVCPTFE DYYTMRNNS 
      NCWYDFTAE FDVPTVIRL NGYPVIEYR VPTADGFIL 
      TMFRIPSKN PKALKYPVY LQHGLVATC AYFVGLKRN 
      SLAFVLADA GYDVWLGNY RGTQYSETH INKTVYQQD 
      YWDHSMDEI VAYDFPASF NTILANTDP DGKIIYIGH 
      SLGTTLSLM YAAEFPEVA KETLRMMVL ISPAYTLAN 
      MKSPYRLAA PFGAAIMNI VGELEMFRI VSQAQPLKV 
      LTDTLCLES PPLMQFCLQ LYNLFYGPH TDFGPEMIP 
      VYFNQLPGG TALKILNHA ADLVLGNFR KYNYVDRNV 
      LYYGTEEPP EYDIKKIQV PVYIIYSSS DWATTAPDA 
      VNLWNHLSE EARFGLKNV EVFNHIDFV YGRHARSLV 
      YDDLVQVLN KFVNKPSGN PSVIDGITM KSVFVLFVL 
      CVPCVLSAR YVPKWKKQA CELPTSQNE HSHYVCDDD 
      GEVKCLPGW TGDLCDVPK CRPGCDPLQ GYCNRPGEC 
      LCKLGYFGE KCNKCIPLP GCQHGYCNV SFECICHEG 
      WDGIFCSEP ICRLDCHST RGYCDSPGE CRCRLGWAG 
      RNCKDCQVL PGCQHGYCD KPLECKCLP GYTGILCQT 
      PICSKTCHK ERGYCRKPG ECRCKVGWW GNNCEKCYP 
      YPGCVNGNC TRPWECNCK KGWGGMLCD EELNYCETN 
      PDTCKNGAK CVSLTKDDG NYKCLCREG TWGKNCEQS 
      EFSTTVKTP TTTTVAGFN KTKQKTEPL LVDNET
    CDS sequence
    • >TC007264 RA
      atgttctta gtgatagtg ctactaggg tgtttgatc 
      caaaacagt ttaagtgaa tacaacaat gtctgtccg 
      acttttgag gattactac acgatgagg aacaactcg 
      aattgttgg tacgacttc acggcggaa ttcgacgtc 
      cccactgtt atacgcctt aacggttat cccgtcatc 
      gagtacaga gtcccaact gccgatgga tttatccta 
      acgatgttc cggattccc tctaaaaac cccaaagcc 
      ctgaaatat ccggtttat ctccaacac ggcctcgtg 
      gcaacttgt gcctacttt gtaggcttg aagaggaac 
      tctctggcg tttgtgttg gccgatgcg gggtatgat 
      gtctggttg gggaattat agaggaaca cagtattcg 
      gaaacgcac atcaataaa acagtgtat cagcaagat 
      tactgggac catagtatg gacgaaatc gtggcgtac 
      gatttccca gcttcgttt aacacgatt ttagcaaat 
      actgacccc gatggcaaa attatttac attgggcat 
      tcgttaggc accactttg tccctaatg tatgcagcg 
      gagtttccc gaagttgcg aaagagacg ctcaggatg 
      atggtcctg atttcgcca gcgtatact ctggctaat 
      atgaagtcg ccttacagg ctggcagca ccctttgga 
      gccgctatt atgaacatt gttggcgaa ttggaaatg 
      ttccggatt gtttctcaa gcccaaccg ttgaaggtg 
      ctcaccgac actctatgt ttggagtca ccgcctctg 
      atgcagttc tgcttgcaa ctctacaat ttgttctac 
      ggcccacat accgatttt ggacctgaa atgatcccg 
      gtttatttc aaccagctt ccgggggga acagcgttg 
      aagattttg aaccatgct gctgacttg gttctggga 
      aatttcagg aaatataac tacgttgat aggaatgtc 
      ctgtactac gggacggag gaaccgcca gagtatgac 
      ataaagaag attcaagtt cctgtgtat attatctac 
      agctcgtcg gattgggct acgacagca ccagacgcg 
      gtgaacttg tggaaccac ctttcggag gaggcgaga 
      ttcggcttg aagaacgtg gaggtgttc aaccacatc 
      gactttgtt tacggaaga cacgcccga agccttgtt 
      tatgatgac ttagttcaa gttttaaat aaattcgtt 
      aacaaaccg tctggaaac ccatcagtg attgatgga 
      atcaccatg aaaagcgtt ttcgtcctt ttcgtgctt 
      tgtgtgccg tgtgttttg tccgcccgt tacgtgccc 
      aaatggaag aaacaggct tgcgaatta cccacttcg 
      caaaatgag cattcgcat tatgtgtgt gacgacgat 
      ggggaggtg aaatgtctg ccaggatgg accggggat 
      ttgtgcgat gtgcccaaa tgccggcct ggatgcgat 
      ccgctgcaa ggatactgc aacaggccg ggggaatgt 
      ttgtgcaag ttggggtat ttcggtgaa aagtgcaat 
      aaatgcatt cctttgccc gggtgtcag catggctat 
      tgcaatgtg agctttgag tgcatctgc cacgaggga 
      tgggatggg atcttttgt tcagaaccg atctgtcgt 
      ttggattgc cactcaacg aggggttat tgcgacagt 
      cctggcgaa tgcagatgt cgcctgggc tgggcagga 
      cgaaactgc aaggactgc caggtcctg ccaggatgt 
      cagcacgga tactgcgac aagcctctg gaatgcaaa 
      tgcctgccc ggatacacc gggattttg tgccagact 
      ccaatctgt tcgaagacg tgccacaag gagcgcgga 
      tactgcagg aaaccgggc gagtgtcga tgcaaagtg 
      ggttggtgg ggaaataat tgtgagaaa tgttatcct 
      tacccaggc tgcgtgaac ggcaattgc acgagaccg 
      tgggagtgt aattgcaag aagggttgg ggagggatg 
      ctctgcgat gaagaactg aactactgc gaaactaat 
      cctgacacg tgtaaaaat ggggcgaaa tgtgtttcg 
      ctcacaaag gatgacggc aattacaag tgtctgtgt 
      cgggaaggt acatggggg aaaaattgc gaacaatct 
      gaattttcc accaccgtt aagacgccc acaactacc 
      accgttgca ggatttaac aaaacgaag caaaaaacc 
      gaaccgctc ttggtggat aatgagacc taa
    mRNA sequence
    • >TC007264 RA
      taaagtgca atgaaacta acccaacat gttcttagt 
      gatagtgct actagggtg tttgatcca aaacagttt 
      aagtgaata caacaatgt ctgtccgac ttttgagga 
      ttactacac gatgaggaa caactcgaa ttgttggta 
      cgacttcac ggcggaatt cgacgtccc cactgttat 
      acgccttaa cggttatcc cgtcatcga gtacagagt 
      cccaactgc cgatggatt tatcctaac gatgttccg 
      gattccctc taaaaaccc caaagccct gaaatatcc 
      ggtttatct ccaacacgg cctcgtggc aacttgtgc 
      ctactttgt aggcttgaa gaggaactc tctggcgtt 
      tgtgttggc cgatgcggg gtatgatgt ctggttggg 
      gaattatag aggaacaca gtattcgga aacgcacat 
      caataaaac agtgtatca gcaagatta ctgggacca 
      tagtatgga cgaaatcgt ggcgtacga tttcccagc 
      ttcgtttaa cacgatttt agcaaatac tgaccccga 
      tggcaaaat tatttacat tgggcattc gttaggcac 
      cactttgtc cctaatgta tgcagcgga gtttcccga 
      agttgcgaa agagacgct caggatgat ggtcctgat 
      ttcgccagc gtatactct ggctaatat gaagtcgcc 
      ttacaggct ggcagcacc ctttggagc cgctattat 
      gaacattgt tggcgaatt ggaaatgtt ccggattgt 
      ttctcaagc ccaaccgtt gaaggtgct caccgacac 
      tctatgttt ggagtcacc gcctctgat gcagttctg 
      cttgcaact ctacaattt gttctacgg cccacatac 
      cgattttgg acctgaaat gatcccggt ttatttcaa 
      ccagcttcc ggggggaac agcgttgaa gattttgaa 
      ccatgctgc tgacttggt tctgggaaa tttcaggaa 
      atataacta cgttgatag gaatgtcct gtactacgg 
      gacggagga accgccaga gtatgacat aaagaagat 
      tcaagttcc tgtgtatat tatctacag ctcgtcgga 
      ttgggctac gacagcacc agacgcggt gaacttgtg 
      gaaccacct ttcggagga ggcgagatt cggcttgaa 
      gaacgtgga ggtgttcaa ccacatcga ctttgttta 
      cggaagaca cgcccgaag ccttgttta tgatgactt 
      agttcaagt tttaaataa attcgttaa caaaccgtc 
      tggaaaccc atcagtgat tgatggaat caccatgaa 
      aagcgtttt cgtcctttt cgtgctttg tgtgccgtg 
      tgttttgtc cgcccgtta cgtgcccaa atggaagaa 
      acaggcttg cgaattacc cacttcgca aaatgagca 
      ttcgcatta tgtgtgtga cgacgatgg ggaggtgaa 
      atgtctgcc aggatggac cggggattt gtgcgatgt 
      gcccaaatg ccggcctgg atgcgatcc gctgcaagg 
      atactgcaa caggccggg ggaatgttt gtgcaagtt 
      ggggtattt cggtgaaaa gtgcaataa atgcattcc 
      tttgcccgg gtgtcagca tggctattg caatgtgag 
      ctttgagtg catctgcca cgagggatg ggatgggat 
      cttttgttc agaaccgat ctgtcgttt ggattgcca 
      ctcaacgag gggttattg cgacagtcc tggcgaatg 
      cagatgtcg cctgggctg ggcaggacg aaactgcaa 
      ggactgcca ggtcctgcc aggatgtca gcacggata 
      ctgcgacaa gcctctgga atgcaaatg cctgcccgg 
      atacaccgg gattttgtg ccagactcc aatctgttc 
      gaagacgtg ccacaagga gcgcggata ctgcaggaa 
      accgggcga gtgtcgatg caaagtggg ttggtgggg 
      aaataattg tgagaaatg ttatcctta cccaggctg 
      cgtgaacgg caattgcac gagaccgtg ggagtgtaa 
      ttgcaagaa gggttgggg agggatgct ctgcgatga 
      agaactgaa ctactgcga aactaatcc tgacacgtg 
      taaaaatgg ggcgaaatg tgtttcgct cacaaagga 
      tgacggcaa ttacaagtg tctgtgtcg ggaaggtac 
      atgggggaa aaattgcga acaatctga attttccac 
      caccgttaa gacgcccac aactaccac cgttgcagg 
      atttaacaa aacgaagca aaaaaccga accgctctt 
      ggtggataa tgagaccta acgaggaac ttgatacgg 
      tttcgttca ttattcgta gcaacgact tggaggcca 
      tagaagcac aatatttat tcactcact aatttatta 
      ttatttatt gtcgttttt attgcaaac tgttacatc 
      ttttatctg taataaaaa cataaaatt gag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY