Status
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Gene information
General information
TC007258 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6753482..6757072
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007258
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04302

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect, flour poorly removed,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva anterior mostlynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007258 RA
      MWLLLHATL FLPVLINAL PTNVAKSSS 
VSASFESSA AAPLLSFDN GNVGVNFLG 
YHASAGLGG LLTGNAADG GLHAEAGTP 
FGQQAGAGL GGTVDASGN RSGYKYHYS 
SSRLDPLNI LPLIRRPSV QTVQKKQKS 
SEVETLD
    • >TC007258 RB
      MWLLLHATL FLPVLINAL PTNVAKSSS VSASFESSA 
AAPLLSFDN GNVGVNFLG YHASAGLGG LLTGNAADG 
GLHAEAGTP FGQQAGAGL GGTVDASGR AAGGLYAGA 
TSGAGASAG AGLGGATGA SGSVGGSFA GASSGGVET 
HITKISHKA PAVATGKLT VKTSAPVVH KEVEVVATP 
VIQKEVEVV AEQPKPQTT YIERTVIPN YVEKKIQVP 
SYVEKTIRV PTTVEKTIR VPAQPTVIE KEVSAPASV 
GVRGSYAAE VETVPKTVT YVQKTKVHG HHHPHVHQR 
VHYKNVGFG GGGGGFGGY GGGFGGFGY GGGYGGGYG 
GGGGASGSY TATVTKTAN PQFFNDIFN IPISTLGAV 
NKLVSGIAG GASGSLSVS KSVGASAY
    • >TC007258 RC
      MKNALSGRA AGGLYAGAT SGAGASAGA GLGGATGAS 
GSVGGSFAG ASSGGVETH ITKISHKAP AVATGKLTV 
KTSAPVVHK EVEVVATPV IQKEVEVVA EQPKPQTTY 
IERTVIPNY VEKKIQVPS YVEKTIRVP TTVEKTIRV 
PAQPTVIEK EVSAPASVG VRGSYAAEV ETVPKTVTY 
VQKTKVHGH HHPHVHQRV HYKNVGFGG GGGGFGGYG 
GGFGGFGYG GGYGGGYGG GGGASGSYT ATVTKTANP 
QFFNDIFNI PISTLGAVN KLVSGIAGG ASGSLSVSK 
SVGASAY
    CDS sequence
    • >TC007258 RA
      atgtggtta cttctacac gcaacttta ttcctcccg 
gttttaata aacgcgctt ccaacaaac gttgcgaaa 
tcctcatcg gtttcagca agttttgaa agtagtgct 
gccgctccg ttactttct tttgataat ggaaacgtc 
ggtgttaac ttcctcggt taccacgct tctgctggt 
ctcgggggt cttttgact ggaaacgca gctgatggg 
ggacttcat gctgaagct ggaactcct ttcggacaa 
caagccgga gctggactt ggaggaaca gtagatgca 
agcggtaat agatccggt tataagtat cattactcg 
tcttcgaga ctggatcct cttaatatt cttccgctt 
attagacga cccagtgtt caaacagta caaaagaaa 
cagaaaagt agcgaagta gaaactctc gattag
    • >TC007258 RB
      atgtggtta cttctacac gcaacttta ttcctcccg 
gttttaata aacgcgctt ccaacaaac gttgcgaaa 
tcctcatcg gtttcagca agttttgaa agtagtgct 
gccgctccg ttactttct tttgataat ggaaacgtc 
ggtgttaac ttcctcggt taccacgct tctgctggt 
ctcgggggt cttttgact ggaaacgca gctgatggg 
ggacttcat gctgaagct ggaactcct ttcggacaa 
caagccgga gctggactt ggaggaaca gtagatgca 
agcggcaga gctgctggc ggcttgtac gccggagcg 
acttccggg gcgggtgct tctgccgga gcaggtctc 
gggggagcc accggagcc tccgggtca gtcggggga 
tcatttgcc ggagcttca tcgggtggt gttgaaacc 
cacataacg aaaatatcg cacaaagct ccggccgta 
gccacaggc aagctaacc gtaaaaacc tcagctccc 
gtcgtccac aaagaagtc gaagttgtt gcaactcca 
gttatccaa aaggaagtc gaagtagtg gccgaacaa 
cccaaaccc caaacaact tacatcgaa agaacggtt 
attcctaac tacgtcgag aagaaaatt caagttcct 
agctacgtg gaaaagaca attcgagtt cctacaacc 
gtcgagaaa accataaga gtgcctgcg caacctact 
gttatcgag aaggaagtt tcggctcct gcgtcggtt 
ggagttcgg gggagttac gctgctgag gtcgagact 
gtgccaaaa acagtaacg tacgtgcaa aaaactaaa 
gtacacgga caccatcac cctcatgtt catcaacgt 
gtacattat aagaatgtt gggtttggt ggaggtggc 
ggcgggttt ggaggctac gggggcggt tttggagga 
tttggatac ggcggaggg tacgggggt ggttatgga 
ggtggcggc ggagcgtcc ggaagttac actgccact 
gttaccaaa actgccaat ccgcaattt ttcaatgat 
atttttaat atcccaatc tctacccta ggggcggtt 
aataagtta gtcagtggt attgcggga ggggctagt 
ggatctctt tctgtgtca aaatcagtt ggagcttct 
gcatattaa
    • >TC007258 RC
      atgaaaaat gccctttca ggcagagct gctggcggc 
ttgtacgcc ggagcgact tccggggcg ggtgcttct 
gccggagca ggtctcggg ggagccacc ggagcctcc 
gggtcagtc gggggatca tttgccgga gcttcatcg 
ggtggtgtt gaaacccac ataacgaaa atatcgcac 
aaagctccg gccgtagcc acaggcaag ctaaccgta 
aaaacctca gctcccgtc gtccacaaa gaagtcgaa 
gttgttgca actccagtt atccaaaag gaagtcgaa 
gtagtggcc gaacaaccc aaaccccaa acaacttac 
atcgaaaga acggttatt cctaactac gtcgagaag 
aaaattcaa gttcctagc tacgtggaa aagacaatt 
cgagttcct acaaccgtc gagaaaacc ataagagtg 
cctgcgcaa cctactgtt atcgagaag gaagtttcg 
gctcctgcg tcggttgga gttcggggg agttacgct 
gctgaggtc gagactgtg ccaaaaaca gtaacgtac 
gtgcaaaaa actaaagta cacggacac catcaccct 
catgttcat caacgtgta cattataag aatgttggg 
tttggtgga ggtggcggc gggtttgga ggctacggg 
ggcggtttt ggaggattt ggatacggc ggagggtac 
gggggtggt tatggaggt ggcggcgga gcgtccgga 
agttacact gccactgtt accaaaact gccaatccg 
caatttttc aatgatatt tttaatatc ccaatctct 
accctaggg gcggttaat aagttagtc agtggtatt 
gcgggaggg gctagtgga tctctttct gtgtcaaaa 
tcagttgga gcttctgca tattaa
    mRNA sequence
    • >TC007258 RA
      tgttggttt cttcatagt ggttcactt gtgattagt 
gctagcgag aagggataa ttaagggta aaaatgtgg 
ttacttcta cacgcaact ttattcctc ccggtttta 
ataaacgcg cttccaaca aacgttgcg aaatcctca 
tcggtttca gcaagtttt gaaagtagt gctgccgct 
ccgttactt tcttttgat aatggaaac gtcggtgtt 
aacttcctc ggttaccac gcttctgct ggtctcggg 
ggtcttttg actggaaac gcagctgat gggggactt 
catgctgaa gctggaact cctttcgga caacaagcc 
ggagctgga cttggagga acagtagat gcaagcggt 
aatagatcc ggttataag tatcattac tcgtcttcg 
agactggat cctcttaat attcttccg cttattaga 
cgacccagt gttcaaaca gtacaaaag aaacagaaa 
agtagcgaa gtagaaact ctcgattag tctttgcac 
gaaaattgt aacgcacca aataatttt atcaataaa 
aggtagaca acacgattt aatgaccac ccaaaaaaa 
taggtggaa atgaataaa taaagttgt cgagaatac
    • >TC007258 RB
      tgttggttt cttcatagt ggttcactt gtgattagt 
gctagcgag aagggataa ttaaggctc attaaaatg 
tataggaag tctcgtaga agcaattgt gtgataaca 
ataattaag ttaattggg atgagcacg gagatctag 
tatctgaca ataaaatac agtaatggt catgcgaaa 
caatcagac agtgtttgt tccaattat cgaactttg 
ctttgatcg agttgatac tgtggaccc gttcaatga 
aattttcac cgttgattc atcaaagta atttaatgt 
cgtaagtgg tatgcataa agagttgta cttttaaat 
gacttgtta atccttgaa aaagcaaca ataaaaggt 
ttgtttgta cccatttca cgtccaact ctgttcacc 
actcacaaa agaaacgtc ttccgtccc aacctccaa 
aaatataaa gtatcacaa cgcgcttat tacattcca 
ggtaaaaat gtggttact tctacacgc aactttatt 
cctcccggt tttaataaa cgcgcttcc aacaaacgt 
tgcgaaatc ctcatcggt ttcagcaag ttttgaaag 
tagtgctgc cgctccgtt actttcttt tgataatgg 
aaacgtcgg tgttaactt cctcggtta ccacgcttc 
tgctggtct cgggggtct tttgactgg aaacgcagc 
tgatggggg acttcatgc tgaagctgg aactccttt 
cggacaaca agccggagc tggacttgg aggaacagt 
agatgcaag cggcagagc tgctggcgg cttgtacgc 
cggagcgac ttccggggc gggtgcttc tgccggagc 
aggtctcgg gggagccac cggagcctc cgggtcagt 
cgggggatc atttgccgg agcttcatc gggtggtgt 
tgaaaccca cataacgaa aatatcgca caaagctcc 
ggccgtagc cacaggcaa gctaaccgt aaaaacctc 
agctcccgt cgtccacaa agaagtcga agttgttgc 
aactccagt tatccaaaa ggaagtcga agtagtggc 
cgaacaacc caaacccca aacaactta catcgaaag 
aacggttat tcctaacta cgtcgagaa gaaaattca 
agttcctag ctacgtgga aaagacaat tcgagttcc 
tacaaccgt cgagaaaac cataagagt gcctgcgca 
acctactgt tatcgagaa ggaagtttc ggctcctgc 
gtcggttgg agttcgggg gagttacgc tgctgaggt 
cgagactgt gccaaaaac agtaacgta cgtgcaaaa 
aactaaagt acacggaca ccatcaccc tcatgttca 
tcaacgtgt acattataa gaatgttgg gtttggtgg 
aggtggcgg cgggtttgg aggctacgg gggcggttt 
tggaggatt tggatacgg cggagggta cgggggtgg 
ttatggagg tggcggcgg agcgtccgg aagttacac 
tgccactgt taccaaaac tgccaatcc gcaattttt 
caatgatat ttttaatat cccaatctc taccctagg 
ggcggttaa taagttagt cagtggtat tgcgggagg 
ggctagtgg atctctttc tgtgtcaaa atcagttgg 
agcttctgc atattaagt ttttgatat gtgatattt 
tagtattta gaagtattt aaatttcag atgtaccta 
caaaatttt ttactcaat aaaatggaa aatagact
    • >TC007258 RC
      ttattgtta aaatgtttg ttacgtgat tacacaacg 
agtttaaaa atgaaaaat gccctttca ggcagagct 
gctggcggc ttgtacgcc ggagcgact tccggggcg 
ggtgcttct gccggagca ggtctcggg ggagccacc 
ggagcctcc gggtcagtc gggggatca tttgccgga 
gcttcatcg ggtggtgtt gaaacccac ataacgaaa 
atatcgcac aaagctccg gccgtagcc acaggcaag 
ctaaccgta aaaacctca gctcccgtc gtccacaaa 
gaagtcgaa gttgttgca actccagtt atccaaaag 
gaagtcgaa gtagtggcc gaacaaccc aaaccccaa 
acaacttac atcgaaaga acggttatt cctaactac 
gtcgagaag aaaattcaa gttcctagc tacgtggaa 
aagacaatt cgagttcct acaaccgtc gagaaaacc 
ataagagtg cctgcgcaa cctactgtt atcgagaag 
gaagtttcg gctcctgcg tcggttgga gttcggggg 
agttacgct gctgaggtc gagactgtg ccaaaaaca 
gtaacgtac gtgcaaaaa actaaagta cacggacac 
catcaccct catgttcat caacgtgta cattataag 
aatgttggg tttggtgga ggtggcggc gggtttgga 
ggctacggg ggcggtttt ggaggattt ggatacggc 
ggagggtac gggggtggt tatggaggt ggcggcgga 
gcgtccgga agttacact gccactgtt accaaaact 
gccaatccg caatttttc aatgatatt tttaatatc 
ccaatctct accctaggg gcggttaat aagttagtc 
agtggtatt gcgggaggg gctagtgga tctctttct 
gtgtcaaaa tcagttgga gcttctgca tattaagtt 
tttgatatg tgatatttt agtatttag aagtattta 
aatttcaga tgtacctac aaaattttt tactcaata 
aaatggaaa atagact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY