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Gene information
General information
TC007258 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6753482..6757072
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007258
    iBeetle screen iB_04302

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect, flour poorly removed,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva anterior mostlynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007258 RA
      MWLLLHATL FLPVLINAL PTNVAKSSS 
      VSASFESSA AAPLLSFDN GNVGVNFLG 
      YHASAGLGG LLTGNAADG GLHAEAGTP 
      FGQQAGAGL GGTVDASGN RSGYKYHYS 
      SSRLDPLNI LPLIRRPSV QTVQKKQKS 
      SEVETLD
    • >TC007258 RB
      MWLLLHATL FLPVLINAL PTNVAKSSS VSASFESSA 
      AAPLLSFDN GNVGVNFLG YHASAGLGG LLTGNAADG 
      GLHAEAGTP FGQQAGAGL GGTVDASGR AAGGLYAGA 
      TSGAGASAG AGLGGATGA SGSVGGSFA GASSGGVET 
      HITKISHKA PAVATGKLT VKTSAPVVH KEVEVVATP 
      VIQKEVEVV AEQPKPQTT YIERTVIPN YVEKKIQVP 
      SYVEKTIRV PTTVEKTIR VPAQPTVIE KEVSAPASV 
      GVRGSYAAE VETVPKTVT YVQKTKVHG HHHPHVHQR 
      VHYKNVGFG GGGGGFGGY GGGFGGFGY GGGYGGGYG 
      GGGGASGSY TATVTKTAN PQFFNDIFN IPISTLGAV 
      NKLVSGIAG GASGSLSVS KSVGASAY
    • >TC007258 RC
      MKNALSGRA AGGLYAGAT SGAGASAGA GLGGATGAS 
      GSVGGSFAG ASSGGVETH ITKISHKAP AVATGKLTV 
      KTSAPVVHK EVEVVATPV IQKEVEVVA EQPKPQTTY 
      IERTVIPNY VEKKIQVPS YVEKTIRVP TTVEKTIRV 
      PAQPTVIEK EVSAPASVG VRGSYAAEV ETVPKTVTY 
      VQKTKVHGH HHPHVHQRV HYKNVGFGG GGGGFGGYG 
      GGFGGFGYG GGYGGGYGG GGGASGSYT ATVTKTANP 
      QFFNDIFNI PISTLGAVN KLVSGIAGG ASGSLSVSK 
      SVGASAY
    CDS sequence
    • >TC007258 RA
      atgtggtta cttctacac gcaacttta ttcctcccg 
      gttttaata aacgcgctt ccaacaaac gttgcgaaa 
      tcctcatcg gtttcagca agttttgaa agtagtgct 
      gccgctccg ttactttct tttgataat ggaaacgtc 
      ggtgttaac ttcctcggt taccacgct tctgctggt 
      ctcgggggt cttttgact ggaaacgca gctgatggg 
      ggacttcat gctgaagct ggaactcct ttcggacaa 
      caagccgga gctggactt ggaggaaca gtagatgca 
      agcggtaat agatccggt tataagtat cattactcg 
      tcttcgaga ctggatcct cttaatatt cttccgctt 
      attagacga cccagtgtt caaacagta caaaagaaa 
      cagaaaagt agcgaagta gaaactctc gattag
    • >TC007258 RB
      atgtggtta cttctacac gcaacttta ttcctcccg 
      gttttaata aacgcgctt ccaacaaac gttgcgaaa 
      tcctcatcg gtttcagca agttttgaa agtagtgct 
      gccgctccg ttactttct tttgataat ggaaacgtc 
      ggtgttaac ttcctcggt taccacgct tctgctggt 
      ctcgggggt cttttgact ggaaacgca gctgatggg 
      ggacttcat gctgaagct ggaactcct ttcggacaa 
      caagccgga gctggactt ggaggaaca gtagatgca 
      agcggcaga gctgctggc ggcttgtac gccggagcg 
      acttccggg gcgggtgct tctgccgga gcaggtctc 
      gggggagcc accggagcc tccgggtca gtcggggga 
      tcatttgcc ggagcttca tcgggtggt gttgaaacc 
      cacataacg aaaatatcg cacaaagct ccggccgta 
      gccacaggc aagctaacc gtaaaaacc tcagctccc 
      gtcgtccac aaagaagtc gaagttgtt gcaactcca 
      gttatccaa aaggaagtc gaagtagtg gccgaacaa 
      cccaaaccc caaacaact tacatcgaa agaacggtt 
      attcctaac tacgtcgag aagaaaatt caagttcct 
      agctacgtg gaaaagaca attcgagtt cctacaacc 
      gtcgagaaa accataaga gtgcctgcg caacctact 
      gttatcgag aaggaagtt tcggctcct gcgtcggtt 
      ggagttcgg gggagttac gctgctgag gtcgagact 
      gtgccaaaa acagtaacg tacgtgcaa aaaactaaa 
      gtacacgga caccatcac cctcatgtt catcaacgt 
      gtacattat aagaatgtt gggtttggt ggaggtggc 
      ggcgggttt ggaggctac gggggcggt tttggagga 
      tttggatac ggcggaggg tacgggggt ggttatgga 
      ggtggcggc ggagcgtcc ggaagttac actgccact 
      gttaccaaa actgccaat ccgcaattt ttcaatgat 
      atttttaat atcccaatc tctacccta ggggcggtt 
      aataagtta gtcagtggt attgcggga ggggctagt 
      ggatctctt tctgtgtca aaatcagtt ggagcttct 
      gcatattaa
    • >TC007258 RC
      atgaaaaat gccctttca ggcagagct gctggcggc 
      ttgtacgcc ggagcgact tccggggcg ggtgcttct 
      gccggagca ggtctcggg ggagccacc ggagcctcc 
      gggtcagtc gggggatca tttgccgga gcttcatcg 
      ggtggtgtt gaaacccac ataacgaaa atatcgcac 
      aaagctccg gccgtagcc acaggcaag ctaaccgta 
      aaaacctca gctcccgtc gtccacaaa gaagtcgaa 
      gttgttgca actccagtt atccaaaag gaagtcgaa 
      gtagtggcc gaacaaccc aaaccccaa acaacttac 
      atcgaaaga acggttatt cctaactac gtcgagaag 
      aaaattcaa gttcctagc tacgtggaa aagacaatt 
      cgagttcct acaaccgtc gagaaaacc ataagagtg 
      cctgcgcaa cctactgtt atcgagaag gaagtttcg 
      gctcctgcg tcggttgga gttcggggg agttacgct 
      gctgaggtc gagactgtg ccaaaaaca gtaacgtac 
      gtgcaaaaa actaaagta cacggacac catcaccct 
      catgttcat caacgtgta cattataag aatgttggg 
      tttggtgga ggtggcggc gggtttgga ggctacggg 
      ggcggtttt ggaggattt ggatacggc ggagggtac 
      gggggtggt tatggaggt ggcggcgga gcgtccgga 
      agttacact gccactgtt accaaaact gccaatccg 
      caatttttc aatgatatt tttaatatc ccaatctct 
      accctaggg gcggttaat aagttagtc agtggtatt 
      gcgggaggg gctagtgga tctctttct gtgtcaaaa 
      tcagttgga gcttctgca tattaa
    mRNA sequence
    • >TC007258 RA
      tgttggttt cttcatagt ggttcactt gtgattagt 
      gctagcgag aagggataa ttaagggta aaaatgtgg 
      ttacttcta cacgcaact ttattcctc ccggtttta 
      ataaacgcg cttccaaca aacgttgcg aaatcctca 
      tcggtttca gcaagtttt gaaagtagt gctgccgct 
      ccgttactt tcttttgat aatggaaac gtcggtgtt 
      aacttcctc ggttaccac gcttctgct ggtctcggg 
      ggtcttttg actggaaac gcagctgat gggggactt 
      catgctgaa gctggaact cctttcgga caacaagcc 
      ggagctgga cttggagga acagtagat gcaagcggt 
      aatagatcc ggttataag tatcattac tcgtcttcg 
      agactggat cctcttaat attcttccg cttattaga 
      cgacccagt gttcaaaca gtacaaaag aaacagaaa 
      agtagcgaa gtagaaact ctcgattag tctttgcac 
      gaaaattgt aacgcacca aataatttt atcaataaa 
      aggtagaca acacgattt aatgaccac ccaaaaaaa 
      taggtggaa atgaataaa taaagttgt cgagaatac
    • >TC007258 RB
      tgttggttt cttcatagt ggttcactt gtgattagt 
      gctagcgag aagggataa ttaaggctc attaaaatg 
      tataggaag tctcgtaga agcaattgt gtgataaca 
      ataattaag ttaattggg atgagcacg gagatctag 
      tatctgaca ataaaatac agtaatggt catgcgaaa 
      caatcagac agtgtttgt tccaattat cgaactttg 
      ctttgatcg agttgatac tgtggaccc gttcaatga 
      aattttcac cgttgattc atcaaagta atttaatgt 
      cgtaagtgg tatgcataa agagttgta cttttaaat 
      gacttgtta atccttgaa aaagcaaca ataaaaggt 
      ttgtttgta cccatttca cgtccaact ctgttcacc 
      actcacaaa agaaacgtc ttccgtccc aacctccaa 
      aaatataaa gtatcacaa cgcgcttat tacattcca 
      ggtaaaaat gtggttact tctacacgc aactttatt 
      cctcccggt tttaataaa cgcgcttcc aacaaacgt 
      tgcgaaatc ctcatcggt ttcagcaag ttttgaaag 
      tagtgctgc cgctccgtt actttcttt tgataatgg 
      aaacgtcgg tgttaactt cctcggtta ccacgcttc 
      tgctggtct cgggggtct tttgactgg aaacgcagc 
      tgatggggg acttcatgc tgaagctgg aactccttt 
      cggacaaca agccggagc tggacttgg aggaacagt 
      agatgcaag cggcagagc tgctggcgg cttgtacgc 
      cggagcgac ttccggggc gggtgcttc tgccggagc 
      aggtctcgg gggagccac cggagcctc cgggtcagt 
      cgggggatc atttgccgg agcttcatc gggtggtgt 
      tgaaaccca cataacgaa aatatcgca caaagctcc 
      ggccgtagc cacaggcaa gctaaccgt aaaaacctc 
      agctcccgt cgtccacaa agaagtcga agttgttgc 
      aactccagt tatccaaaa ggaagtcga agtagtggc 
      cgaacaacc caaacccca aacaactta catcgaaag 
      aacggttat tcctaacta cgtcgagaa gaaaattca 
      agttcctag ctacgtgga aaagacaat tcgagttcc 
      tacaaccgt cgagaaaac cataagagt gcctgcgca 
      acctactgt tatcgagaa ggaagtttc ggctcctgc 
      gtcggttgg agttcgggg gagttacgc tgctgaggt 
      cgagactgt gccaaaaac agtaacgta cgtgcaaaa 
      aactaaagt acacggaca ccatcaccc tcatgttca 
      tcaacgtgt acattataa gaatgttgg gtttggtgg 
      aggtggcgg cgggtttgg aggctacgg gggcggttt 
      tggaggatt tggatacgg cggagggta cgggggtgg 
      ttatggagg tggcggcgg agcgtccgg aagttacac 
      tgccactgt taccaaaac tgccaatcc gcaattttt 
      caatgatat ttttaatat cccaatctc taccctagg 
      ggcggttaa taagttagt cagtggtat tgcgggagg 
      ggctagtgg atctctttc tgtgtcaaa atcagttgg 
      agcttctgc atattaagt ttttgatat gtgatattt 
      tagtattta gaagtattt aaatttcag atgtaccta 
      caaaatttt ttactcaat aaaatggaa aatagact
    • >TC007258 RC
      ttattgtta aaatgtttg ttacgtgat tacacaacg 
      agtttaaaa atgaaaaat gccctttca ggcagagct 
      gctggcggc ttgtacgcc ggagcgact tccggggcg 
      ggtgcttct gccggagca ggtctcggg ggagccacc 
      ggagcctcc gggtcagtc gggggatca tttgccgga 
      gcttcatcg ggtggtgtt gaaacccac ataacgaaa 
      atatcgcac aaagctccg gccgtagcc acaggcaag 
      ctaaccgta aaaacctca gctcccgtc gtccacaaa 
      gaagtcgaa gttgttgca actccagtt atccaaaag 
      gaagtcgaa gtagtggcc gaacaaccc aaaccccaa 
      acaacttac atcgaaaga acggttatt cctaactac 
      gtcgagaag aaaattcaa gttcctagc tacgtggaa 
      aagacaatt cgagttcct acaaccgtc gagaaaacc 
      ataagagtg cctgcgcaa cctactgtt atcgagaag 
      gaagtttcg gctcctgcg tcggttgga gttcggggg 
      agttacgct gctgaggtc gagactgtg ccaaaaaca 
      gtaacgtac gtgcaaaaa actaaagta cacggacac 
      catcaccct catgttcat caacgtgta cattataag 
      aatgttggg tttggtgga ggtggcggc gggtttgga 
      ggctacggg ggcggtttt ggaggattt ggatacggc 
      ggagggtac gggggtggt tatggaggt ggcggcgga 
      gcgtccgga agttacact gccactgtt accaaaact 
      gccaatccg caatttttc aatgatatt tttaatatc 
      ccaatctct accctaggg gcggttaat aagttagtc 
      agtggtatt gcgggaggg gctagtgga tctctttct 
      gtgtcaaaa tcagttgga gcttctgca tattaagtt 
      tttgatatg tgatatttt agtatttag aagtattta 
      aatttcaga tgtacctac aaaattttt tactcaata 
      aaatggaaa atagact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY