| General information | |||
| TC007253 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 6814623..6879647 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007253 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04300 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
egg chamber mostly not present - Number of animals showing the phenotype: 2
previtellogenic egg chamber potentially fused - Number of animals showing the phenotype: 2
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007253 RA
MLCRVAFFV FVAIITAAL ADRQPQLTI QPSTPVVSK EVGGKLALT CAPDVEESS LISQLEWRD SKNRRIEST NNGKKTFSQ SNGRDLILF FQELQERDA GNYTCVANY GSETLKSTV VVNTYVDIT FVNAPESQY PKVGEDYLV RCKVHGNPT PIVEWSKKD NKIESNEKY VIQSDGLLI KNPTEADDG SYTCRAYAV STGGFKERV IKLEVIIPP VIKDMEPVS VIEGEQAWT KCEATGKPP PTYSWIKQR NSQDLSKSD RFTVKQTTG NLLISKVEF NDDDFYKCT AENKAGRAE KTVKINVLV KPKIFELLN VTAPVHQET KIICKARGR PTPKVYFRK VSDKEHPFR VGQQRDPRI ILEEQMFEK TGETFGTLI ISNLNRSDD GLYECIAEN SAGTGYKNG HITVEFPPT FERTKLLEP IAWTWLNHP GNLTCIPEA IPNATVVWK HNGYIINPG NPPFKIQGF GPTSHLIVD AFNDPRFYG EYECLATNK LGKATHSIT LKEAQVPAN VDKVQIQTL TATTIKFNI VPPQYFDTL PIRSFTVQY KPEGEIGWS RARNRTWSF RAPYILENL VPEVTYNFR FAAANDVGF GPWTNAPTV TMPRRSEPA EPKIYVPNH ILDNSSNRQ DVVAVSPYA DHFELRWQV PNDNGDPID VYLIRYCVI TKINGEWRD SDCSEEIQQ TFKSTTYDL NSLKPDTFY KIELRAHNS IGSSSPAQI KVKTARGID PAVPYNEPS VTNFAIIGI VVGAVILVL ILVDLICFC VNRAGILAF ICDRNRSKH HEEDPKLGR EEEREPLKD AHMNGGEKT MSVEFDGKH VYSKSGEII GKHSAV
- >TC007253 RB
MLCRVAFFV FVAIITAAL ADRQPQLTI QPSTPVVSK EVGGKLALT CAPDVEESS LISQLEWRD SKNRRIEST NNGKKTFSQ SNGRDLILF FQELQERDA GNYTCVANY GSETLKSTV VVNTYVDIT FVNAPESQY PKVGEDYLV RCKVHGNPT PIVEWSKKD NKIESNEKY VIQSDGLLI KNPTEADDG SYTCRAYAV STGGFKERV IKLEVIIPP VIKDMEPVS VIEGEQAWT KCEATGKPP PTYSWIKQR NSQDLSKSD RFTVKQTTG NLLISKVEF NDDDFYKCT AENKAGRAE KTVKINVLV KPKIFELLN VTAPVHQET KIICKARGR PTPKVYFRK VSDKEHPFR VGQQRDPRI ILEEQMFEK TGETFGTLI ISNLNRSDD GLYECIAEN SAGTGYKNG HITVEFPPT FERTKLLEP IAWTWLNHP GNLTCIPEA IPNATVVWK HNGYIINPG NPPFKIQGF GPTSHLIVD AFNDPRFYG EYECLATNK LGKATHSIT LKEAQVPAN VDKVQIQTL TATTIKFNI VPPQYFDTL PIRSFTVQY KPEGEIGWS RARNRTWSF RAPYILENL VPEVTYNFR FAAANDVGF GPWTNAPTV TMPRRSEPA EPKIYVPNH ILDNSSNRQ DVVAVSPYA DHFELRWQV PNDNGDPID VYLIRYCVI TKINGEWRD SDCSEEIQQ TFKSTTYDL NSLKPDTFY KIELRAHNS IGSSSPAQI KVKTARGID PAVPYNEPS VTNFAIIGI VVGAVILVL ILVDLICFC VNRAGILAF ICDRNRSKH HEEDPKLGS YKAAPAHPN SLNLPLPVK LAPSPMEEE REPLKDAHM NGGEKTMSV EFDGKHVYS KSGEIIGKH SAV
- >TC007253 RA
atgctgtgt cgggtggcg ttttttgtc tttgttgcc atcattaca gcggcgctt gctgacaga caaccgcaa ttgaccatc cagcccagc acaccagtc gtttccaaa gaagttgga ggcaagcta gccttaaca tgtgcacct gacgtggaa gaatcaagt ctcatctcc cagttggaa tggagggat tcaaaaaac cgtagaata gagtccacc aataacggg aaaaagact ttttcacag agtaacggg cgcgaccta atcctcttc ttccaagaa ttacaagag cgagatgcg ggcaactac acttgtgtc gccaattac ggcagtgag accttgaaa tcaaccgtg gtggtcaac acttacgtt gatatcacg tttgtgaac gctcctgag tcccagtac cccaaagtc ggggaggac tacctcgtt aggtgtaaa gtccacggg aaccccaca ccgatcgtc gagtggtcg aagaaagac aataagatt gaatcgaac gaaaagtac gtcatacag agcgatggt ctgctgatc aaaaatccg acggaagcg gacgatggc agctacacg tgccgggct tatgccgtc tcgaccggc ggcttcaaa gagagagtg attaagctg gaagtgatt attcccccg gtgatcaaa gacatggag cctgtttcg gtgatcgag ggcgaacaa gcctggacc aagtgcgaa gccacggga aaaccgcca ccaacttac agctggatc aagcaacgg aacagccaa gacttgtcg aagagtgac cgcttcacc gtcaaacag accaccggg aatctcctc atcagcaaa gtggaattc aacgacgac gatttttac aaatgcacg gcggagaac aaggccgga cgtgccgaa aaaaccgtc aaaatcaac gtcttggtc aagccgaaa attttcgaa ttgctcaac gtcacggca cccgtgcac caggaaacg aagattatc tgcaaggca aggggacgg cccacacct aaagtttac ttccggaaa gtgagcgat aaggagcac ccgttccgg gtgggccaa cagcgcgac ccccggatt attctggag gagcagatg ttcgagaaa acgggggag acgtttggc acgcttatc ataagcaac ttgaacagg tcggacgac gggctgtac gagtgcatt gccgagaac agcgctggg accggctac aaaaacggt cacattact gttgaattt ccgccgact tttgagcgc accaagctc ctggagccc atcgcctgg acgtggctc aaccacccc gggaacctc acgtgcatc ccggaagcc atcccgaac gccacagtg gtgtggaag cacaacggc tacatcata aacccgggg aaccccccg tttaaaatc cagggtttc gggcccacc tctcacctg attgttgac gcgttcaac gacccgcgt ttttacggc gagtacgag tgtctggcg accaacaag ctggggaag gcgacgcac tcgatcacg ctaaaggag gcccaagtg cccgcaaat gtggacaag gtgcagatt caaaccctc acggccact actatcaaa ttcaacatt gttccacct cagtacttt gatacgctc ccgattcgg tcgtttacg gttcagtat aagcctgaa ggggagatt gggtggtcg cgtgccagg aacaggacg tggagtttt agggcaccg tatattttg gagaatttg gtgccggaa gtgacttat aattttcga tttgcggcg gctaatgat gtgggattt gggccgtgg acgaatgcg cccacggtg acgatgcca aggcggtcg gaaccggcc gagccgaag atttatgta cctaatcat attttggat aatagtagt aataggcag gatgtggtt gctgtgagt ccctatgcg gaccatttc gagttgagg tggcaagtg ccgaatgat aacggcgat ccgattgat gtttatctc ataagatat tgtgtgata acgaaaatc aacggcgag tggcgcgac agcgactgc agcgaagag atccagcaa acgttcaaa tcgaccact tatgattta aactcgttg aaacccgat acgttctac aaaatcgaa ctgcgtgct cacaactca atcggttca agttcaccg gctcaaatc aaagtgaaa acagccaga ggcatcgac cctgcagtg ccctacaac gagccttca gtcactaat ttcgcaata attggaatc gtggtggga gcagtcatt cttgtgcta attctcgtc gacttaatc tgtttttgc gtgaaccgc gcgggaatt ctcgcgttt atctgcgat cgaaaccga tcgaagcac catgaagag gacccgaaa ctaggaagg gaggaagag cgtgagcct ctaaaggac gcccacatg aacggagga gagaaaacc atgtccgtg gaattcgac ggcaagcac gtctactca aaatcgggc gaaataatc ggcaaacat tcggccgtt taa
- >TC007253 RB
atgctgtgt cgggtggcg ttttttgtc tttgttgcc atcattaca gcggcgctt gctgacaga caaccgcaa ttgaccatc cagcccagc acaccagtc gtttccaaa gaagttgga ggcaagcta gccttaaca tgtgcacct gacgtggaa gaatcaagt ctcatctcc cagttggaa tggagggat tcaaaaaac cgtagaata gagtccacc aataacggg aaaaagact ttttcacag agtaacggg cgcgaccta atcctcttc ttccaagaa ttacaagag cgagatgcg ggcaactac acttgtgtc gccaattac ggcagtgag accttgaaa tcaaccgtg gtggtcaac acttacgtt gatatcacg tttgtgaac gctcctgag tcccagtac cccaaagtc ggggaggac tacctcgtt aggtgtaaa gtccacggg aaccccaca ccgatcgtc gagtggtcg aagaaagac aataagatt gaatcgaac gaaaagtac gtcatacag agcgatggt ctgctgatc aaaaatccg acggaagcg gacgatggc agctacacg tgccgggct tatgccgtc tcgaccggc ggcttcaaa gagagagtg attaagctg gaagtgatt attcccccg gtgatcaaa gacatggag cctgtttcg gtgatcgag ggcgaacaa gcctggacc aagtgcgaa gccacggga aaaccgcca ccaacttac agctggatc aagcaacgg aacagccaa gacttgtcg aagagtgac cgcttcacc gtcaaacag accaccggg aatctcctc atcagcaaa gtggaattc aacgacgac gatttttac aaatgcacg gcggagaac aaggccgga cgtgccgaa aaaaccgtc aaaatcaac gtcttggtc aagccgaaa attttcgaa ttgctcaac gtcacggca cccgtgcac caggaaacg aagattatc tgcaaggca aggggacgg cccacacct aaagtttac ttccggaaa gtgagcgat aaggagcac ccgttccgg gtgggccaa cagcgcgac ccccggatt attctggag gagcagatg ttcgagaaa acgggggag acgtttggc acgcttatc ataagcaac ttgaacagg tcggacgac gggctgtac gagtgcatt gccgagaac agcgctggg accggctac aaaaacggt cacattact gttgaattt ccgccgact tttgagcgc accaagctc ctggagccc atcgcctgg acgtggctc aaccacccc gggaacctc acgtgcatc ccggaagcc atcccgaac gccacagtg gtgtggaag cacaacggc tacatcata aacccgggg aaccccccg tttaaaatc cagggtttc gggcccacc tctcacctg attgttgac gcgttcaac gacccgcgt ttttacggc gagtacgag tgtctggcg accaacaag ctggggaag gcgacgcac tcgatcacg ctaaaggag gcccaagtg cccgcaaat gtggacaag gtgcagatt caaaccctc acggccact actatcaaa ttcaacatt gttccacct cagtacttt gatacgctc ccgattcgg tcgtttacg gttcagtat aagcctgaa ggggagatt gggtggtcg cgtgccagg aacaggacg tggagtttt agggcaccg tatattttg gagaatttg gtgccggaa gtgacttat aattttcga tttgcggcg gctaatgat gtgggattt gggccgtgg acgaatgcg cccacggtg acgatgcca aggcggtcg gaaccggcc gagccgaag atttatgta cctaatcat attttggat aatagtagt aataggcag gatgtggtt gctgtgagt ccctatgcg gaccatttc gagttgagg tggcaagtg ccgaatgat aacggcgat ccgattgat gtttatctc ataagatat tgtgtgata acgaaaatc aacggcgag tggcgcgac agcgactgc agcgaagag atccagcaa acgttcaaa tcgaccact tatgattta aactcgttg aaacccgat acgttctac aaaatcgaa ctgcgtgct cacaactca atcggttca agttcaccg gctcaaatc aaagtgaaa acagccaga ggcatcgac cctgcagtg ccctacaac gagccttca gtcactaat ttcgcaata attggaatc gtggtggga gcagtcatt cttgtgcta attctcgtc gacttaatc tgtttttgc gtgaaccgc gcgggaatt ctcgcgttt atctgcgat cgaaaccga tcgaagcac catgaagag gacccgaaa ctaggaagt tacaaagct gccccggcg catcccaac agtctcaac ttgccccta cctgtcaaa ctggcacca tcaccaatg gaggaagag cgtgagcct ctaaaggac gcccacatg aacggagga gagaaaacc atgtccgtg gaattcgac ggcaagcac gtctactca aaatcgggc gaaataatc ggcaaacat tcggccgtt taa
- >TC007253 RA
acaggattt tagaacagt cgttgcgcg tcagctagt atggatgtg gttatagtt ctttctctc tcgtttgct gcgtttcgt aaacttttc gaacttttt taaaagaaa acgaaccat ttttaccac actaagtga tatttttca agtttttca ttgacttta gtgactttt tataatcca gaatgctgt gtcgggtgg cgttttttg tctttgttg ccatcatta cagcggcgc ttgctgaca gacaaccgc aattgacca tccagccca gcacaccag tcgtttcca aagaagttg gaggcaagc tagccttaa catgtgcac ctgacgtgg aagaatcaa gtctcatct cccagttgg aatggaggg attcaaaaa accgtagaa tagagtcca ccaataacg ggaaaaaga ctttttcac agagtaacg ggcgcgacc taatcctct tcttccaag aattacaag agcgagatg cgggcaact acacttgtg tcgccaatt acggcagtg agaccttga aatcaaccg tggtggtca acacttacg ttgatatca cgtttgtga acgctcctg agtcccagt accccaaag tcggggagg actacctcg ttaggtgta aagtccacg ggaacccca caccgatcg tcgagtggt cgaagaaag acaataaga ttgaatcga acgaaaagt acgtcatac agagcgatg gtctgctga tcaaaaatc cgacggaag cggacgatg gcagctaca cgtgccggg cttatgccg tctcgaccg gcggcttca aagagagag tgattaagc tggaagtga ttattcccc cggtgatca aagacatgg agcctgttt cggtgatcg agggcgaac aagcctgga ccaagtgcg aagccacgg gaaaaccgc caccaactt acagctgga tcaagcaac ggaacagcc aagacttgt cgaagagtg accgcttca ccgtcaaac agaccaccg ggaatctcc tcatcagca aagtggaat tcaacgacg acgattttt acaaatgca cggcggaga acaaggccg gacgtgccg aaaaaaccg tcaaaatca acgtcttgg tcaagccga aaattttcg aattgctca acgtcacgg cacccgtgc accaggaaa cgaagatta tctgcaagg caaggggac ggcccacac ctaaagttt acttccgga aagtgagcg ataaggagc acccgttcc gggtgggcc aacagcgcg acccccgga ttattctgg aggagcaga tgttcgaga aaacggggg agacgtttg gcacgctta tcataagca acttgaaca ggtcggacg acgggctgt acgagtgca ttgccgaga acagcgctg ggaccggct acaaaaacg gtcacatta ctgttgaat ttccgccga cttttgagc gcaccaagc tcctggagc ccatcgcct ggacgtggc tcaaccacc ccgggaacc tcacgtgca tcccggaag ccatcccga acgccacag tggtgtgga agcacaacg gctacatca taaacccgg ggaaccccc cgtttaaaa tccagggtt tcgggccca cctctcacc tgattgttg acgcgttca acgacccgc gtttttacg gcgagtacg agtgtctgg cgaccaaca agctgggga aggcgacgc actcgatca cgctaaagg aggcccaag tgcccgcaa atgtggaca aggtgcaga ttcaaaccc tcacggcca ctactatca aattcaaca ttgttccac ctcagtact ttgatacgc tcccgattc ggtcgttta cggttcagt ataagcctg aaggggaga ttgggtggt cgcgtgcca ggaacagga cgtggagtt ttagggcac cgtatattt tggagaatt tggtgccgg aagtgactt ataattttc gatttgcgg cggctaatg atgtgggat ttgggccgt ggacgaatg cgcccacgg tgacgatgc caaggcggt cggaaccgg ccgagccga agatttatg tacctaatc atattttgg ataatagta gtaataggc aggatgtgg ttgctgtga gtccctatg cggaccatt tcgagttga ggtggcaag tgccgaatg ataacggcg atccgattg atgtttatc tcataagat attgtgtga taacgaaaa tcaacggcg agtggcgcg acagcgact gcagcgaag agatccagc aaacgttca aatcgacca cttatgatt taaactcgt tgaaacccg atacgttct acaaaatcg aactgcgtg ctcacaact caatcggtt caagttcac cggctcaaa tcaaagtga aaacagcca gaggcatcg accctgcag tgccctaca acgagcctt cagtcacta atttcgcaa taattggaa tcgtggtgg gagcagtca ttcttgtgc taattctcg tcgacttaa tctgttttt gcgtgaacc gcgcgggaa ttctcgcgt ttatctgcg atcgaaacc gatcgaagc accatgaag aggacccga aactaggaa gggaggaag agcgtgagc ctctaaagg acgcccaca tgaacggag gagagaaaa ccatgtccg tggaattcg acggcaagc acgtctact caaaatcgg gcgaaataa tcggcaaac attcggccg tttaagatc tgtgttttc aagtgagag cttcactca ttccatgac agttaaaac atagtgcaa caatgcaat ctttgtaaa ctactatat ttttaaaga gaccgcgtg ccaaatgat tgtagctta aatttcata actatttta gcctcagta ttatatcca gacgccgag tcaagtttc tgtgcaatt tggaaaaaa cttgattgg tggtttgtg tacagcttc agtgttatg tgtgtaact aaagggatt atgtataaa tgtttcatt ttgtatagt taattagga tagtttagg taacgctac caaagcact tgtgtatcc gaatttgtt aacagcagc actggcagt acttaacta cagactgag caatttttt caagacccg acattgttt ttattcatt acgttttct agttcgtaa gaaattaag taccaaagt tattatttt agactaatg acatttttt attgtattt atgatataa ttaattata ttgtagctt ttgatatgg aataaagca attaagtac ct
- >TC007253 RB
acaggattt tagaacagt cgttgcgcg tcagctagt atggatgtg gttatagtt ctttctctc tcgtttgct gcgtttcgt aaacttttc gaacttttt taaaagaaa acgaaccat ttttaccac actaagtga tatttttca agtttttca ttgacttta gtgactttt tataatcca gaatgctgt gtcgggtgg cgttttttg tctttgttg ccatcatta cagcggcgc ttgctgaca gacaaccgc aattgacca tccagccca gcacaccag tcgtttcca aagaagttg gaggcaagc tagccttaa catgtgcac ctgacgtgg aagaatcaa gtctcatct cccagttgg aatggaggg attcaaaaa accgtagaa tagagtcca ccaataacg ggaaaaaga ctttttcac agagtaacg ggcgcgacc taatcctct tcttccaag aattacaag agcgagatg cgggcaact acacttgtg tcgccaatt acggcagtg agaccttga aatcaaccg tggtggtca acacttacg ttgatatca cgtttgtga acgctcctg agtcccagt accccaaag tcggggagg actacctcg ttaggtgta aagtccacg ggaacccca caccgatcg tcgagtggt cgaagaaag acaataaga ttgaatcga acgaaaagt acgtcatac agagcgatg gtctgctga tcaaaaatc cgacggaag cggacgatg gcagctaca cgtgccggg cttatgccg tctcgaccg gcggcttca aagagagag tgattaagc tggaagtga ttattcccc cggtgatca aagacatgg agcctgttt cggtgatcg agggcgaac aagcctgga ccaagtgcg aagccacgg gaaaaccgc caccaactt acagctgga tcaagcaac ggaacagcc aagacttgt cgaagagtg accgcttca ccgtcaaac agaccaccg ggaatctcc tcatcagca aagtggaat tcaacgacg acgattttt acaaatgca cggcggaga acaaggccg gacgtgccg aaaaaaccg tcaaaatca acgtcttgg tcaagccga aaattttcg aattgctca acgtcacgg cacccgtgc accaggaaa cgaagatta tctgcaagg caaggggac ggcccacac ctaaagttt acttccgga aagtgagcg ataaggagc acccgttcc gggtgggcc aacagcgcg acccccgga ttattctgg aggagcaga tgttcgaga aaacggggg agacgtttg gcacgctta tcataagca acttgaaca ggtcggacg acgggctgt acgagtgca ttgccgaga acagcgctg ggaccggct acaaaaacg gtcacatta ctgttgaat ttccgccga cttttgagc gcaccaagc tcctggagc ccatcgcct ggacgtggc tcaaccacc ccgggaacc tcacgtgca tcccggaag ccatcccga acgccacag tggtgtgga agcacaacg gctacatca taaacccgg ggaaccccc cgtttaaaa tccagggtt tcgggccca cctctcacc tgattgttg acgcgttca acgacccgc gtttttacg gcgagtacg agtgtctgg cgaccaaca agctgggga aggcgacgc actcgatca cgctaaagg aggcccaag tgcccgcaa atgtggaca aggtgcaga ttcaaaccc tcacggcca ctactatca aattcaaca ttgttccac ctcagtact ttgatacgc tcccgattc ggtcgttta cggttcagt ataagcctg aaggggaga ttgggtggt cgcgtgcca ggaacagga cgtggagtt ttagggcac cgtatattt tggagaatt tggtgccgg aagtgactt ataattttc gatttgcgg cggctaatg atgtgggat ttgggccgt ggacgaatg cgcccacgg tgacgatgc caaggcggt cggaaccgg ccgagccga agatttatg tacctaatc atattttgg ataatagta gtaataggc aggatgtgg ttgctgtga gtccctatg cggaccatt tcgagttga ggtggcaag tgccgaatg ataacggcg atccgattg atgtttatc tcataagat attgtgtga taacgaaaa tcaacggcg agtggcgcg acagcgact gcagcgaag agatccagc aaacgttca aatcgacca cttatgatt taaactcgt tgaaacccg atacgttct acaaaatcg aactgcgtg ctcacaact caatcggtt caagttcac cggctcaaa tcaaagtga aaacagcca gaggcatcg accctgcag tgccctaca acgagcctt cagtcacta atttcgcaa taattggaa tcgtggtgg gagcagtca ttcttgtgc taattctcg tcgacttaa tctgttttt gcgtgaacc gcgcgggaa ttctcgcgt ttatctgcg atcgaaacc gatcgaagc accatgaag aggacccga aactaggaa gttacaaag ctgccccgg cgcatccca acagtctca acttgcccc tacctgtca aactggcac catcaccaa tggaggaag agcgtgagc ctctaaagg acgcccaca tgaacggag gagagaaaa ccatgtccg tggaattcg acggcaagc acgtctact caaaatcgg gcgaaataa tcggcaaac attcggccg tttaagatc tgtgttttc aagtgagag cttcactca ttccatgac agttaaaac atagtgcaa caatgcaat ctttgtaaa ctactatat ttttaaaga gaccgcgtg ccaaatgat tgtagctta aatttcata actatttta gcctcagta ttatatcca gacgccgag tcaagtttc tgtgcaatt tggaaaaaa cttgattgg tggtttgtg tacagcttc agtgttatg tgtgtaact aaagggatt atgtataaa tgtttcatt ttgtatagt taattagga tagtttagg taacgctac caaagcact tgtgtatcc gaatttgtt aacagcagc actggcagt acttaacta cagactgag caatttttt caagacccg acattgttt ttattcatt acgttttct agttcgtaa gaaattaag taccaaagt tattatttt agactaatg acatttttt attgtattt atgatataa ttaattata ttgtagctt ttgatatgg aataaagca attaagtac ct
