| General information | |||
| TC007250 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 6906803..6937902 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007250 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04299 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Days post injection
Fertility
mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: 3 -
Number of animals showing the phenotype: 1
mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 1
ovary without detectable defects - Number of animals showing the phenotype: 2
mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 1
ovary without detectable defects - Number of animals showing the phenotype: 2
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: < 20
embryo/egg not developed/not fertilized -
Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
Days post injection
Pupal morphology
antenna potentially size shortened -
Number of animals showing the phenotype: 7
eyes size decreased - Number of animals showing the phenotype: 6
ommatidia shape irregular - Number of animals showing the phenotype: 6
wings not closed - Number of animals showing the phenotype: 7
legs orientation irregular - Number of animals showing the phenotype: 7
oblique dorsal thoracic muscles size shortened - Number of animals showing the phenotype: 6
oblique dorsal thoracic muscles broader - Number of animals showing the phenotype: 6
eyes size decreased - Number of animals showing the phenotype: 6
ommatidia shape irregular - Number of animals showing the phenotype: 6
wings not closed - Number of animals showing the phenotype: 7
legs orientation irregular - Number of animals showing the phenotype: 7
oblique dorsal thoracic muscles size shortened - Number of animals showing the phenotype: 6
oblique dorsal thoracic muscles broader - Number of animals showing the phenotype: 6
- >TC007250 RA
MIGFIYVRG IYRSSTEDE IIQFRAEIG VTNVVGFTW LLRPEASEE ARKIIADIE EILQSLEYV QAIDKQKFL LEKCRIDEA RIKLVEACT RGQHVNENW HVARKHRLT ASRFGMVLS ACSRRRFPP SLFKNLAEG YSLDRVAAV QWGKTHEKT ALREFEEAT NLKVQETGF WLEESGFLG ASPDGLVEE DGILEIKCP YKYRDTDSL SEALKDKKY FYWRDENED INLNSNHNY YHQVQGQMH ITGRKVIKL ADVYKPKLS NYTALSRSS TEDEIIQFR AEIGVTNVV GFTWLLRPE ASEEARKII ADIEEILQS LEYVQAIDK QKFLLEKCR IDEARIKLV EACTRGQHV NENWHVARK HRLTASRFG MVLSACSRR RFPPSLFKN LAEGYSLDR VAAVQWGKT HEKTALREF EEATNLKVQ ETGFWLEES GFLGASPDG LVEEDGILE IKCPYKYRD TDSLSEALK DKKYFYWRD ENEDINLNS NHNYYHQGC SSMDGFRVI CILVAVLAT TGRATAADH VRMVTKADL ITVPLNFDT SIVCTMNIV PDNFQWKFY PLSRRQASN PKAHISLTH SHFEIIDER KYEITQKKT STLNLQAKS DDIAGDYQC LAYYGASVV ASIPWRLTI ANISEFPPQ EKIDVTVIP GNTVNWRCE PPEANPEPY IDYHKNGVY VPPFVQVVT KSLIIPNVT SGNSGSYTC KAGNVCTEK FSPVLFNLK VDKRAPREP PKFIVRPRS VYNVTKGDK VLLECSAVG NPLPKVVWR KITGIIPEG RSELTPGGL IIKNISASD NGIYECTHD NSLGKVVHS ITLNYNEAP TINCPQDSI DVNQGEDLE LECTVTGNP DPEITWFLN GFVTKDNKI ITEGNKIFF KPTEKRHAG NLQVFARNI AGTVYSSIS IKVIPLSTT QDNVSENPL PPTRLHRPN SRKFNKHAK MYPPSKPNI TGISDEAVV LRWTVQNKG LPIQFFKVQ YKDFGPANQ HDHHNKGKG SKWNTDNPI IEPHIRSYQ VNNLKPDHV YRFRIAAVY SNSDNKNSP NSDKFHLRK LDFDKRNPL PVPLITKTE SINTTAVKI YWKCEPSIN ITIDGFFVN YNSASSAGD YTKATVDGP QTNSFVLSH LQPDTVYDI KLQSFNSKS ASDFSSIMK AKTLGLAST TLSPREPPP SSANTETAG ISKLYVFIA AGVLGACGV LFAAIGLLL FCRKQSKQK KTGTRGDHD KACDDHHHI QADATEYVV SPKSGPRTN GCVAPNRIT ITANPLADA DNKKFLARI FSRIRI
- >TC007250 RB
MIGFIYVRG IYRSSTEDE IIQFRAEIG VTNVVGFTW LLRPEASEE ARKIIADIE EILQSLEYV QAIDKQKFL LEKCRIDEA RIKLVEACT RGQHVNENW HVARKHRLT ASRFGMVLS ACSRRRFPP SLFKNLAEG YSLDRVAAV QWGKTHEKT ALREFEEAT NLKVQETGF WLEESGFLG ASPDGLVEE DGILEIKCP YKYRDTDSL SEALKDKKY FYWRDENED INLNSNHNY YHQVQGQMH ITGRKVIKL ADVYKPKLS NYTALSRSS TEDEIIQFR AEIGVTNVV GFTWLLRPE ASEEARKII ADIEEILQS LEYVQAIDK QKFLLEKCR IDEARIKLV EACTRGQHV NENWHVARK HRLTASRFG MVLSACSRR RFPPSLFKN LAEGYSLDR VAAVQWGKT HEKTALREF EEATNLKVQ ETGFWLEES GFLGASPDG LVEEDGILE IKCPYKYRD TDSLSEALK DKKYFYWRD ENEDINLNS NHNYYHQGC SSMDGFRVI CILVAVLAT TGRATAADH VRMVTKADL ITVPLNFDT SIVCTMNIV PDNFQWKFY PLSRRQASN PKAHISLTH SHFEIIDER KYEITQKKT STLNLQAKS DDIAGDYQC LAYYGASVV ASIPWRLTI ANISEFPPQ EKIDVTVIP GNTVNWRCE PPEANPEPY IDYHKNGVY VPPFVQVVT KSLIIPNVT SGNSGSYTC KAGNVCTEK FSPVLFNLK VDKRAPREP PKFIVRPRS VYNVTKGDK VLLECSAVG NPLPKVVWR KITGIIPEG RSELTPGGL IIKNISASD NGIYECTHD NSLGKVVHS ITLNYNEAP TINCPQDSI DVNQGEDLE LECTVTGNP DPEITWFLN GFVTKDNKI ITEGNKIFF KPTEKRHAG NLQVFARNI AGTVYSSIS IKVIPLSTT QDNVSENPL PPTRLHRPN SRKFNKHAK MYPPSKPNI TGISDEAVV LRWTVQNKG LPIQFFKVQ YKDFGPANQ HDHHNKGKG SKWNTDNPI IEPHIRSYQ VNNLKPDHV YRFRIAAVY SNSDNKNSP NSDKFHLRK LDFDKRNPL PVPLITKTE SINTTAVKI YWKCEPSIN ITIDGFFVN YNSASSAGD YTKATVDGP QTNSFVLSH LQPDTVYDI KLQSFNSKS ASDFSSIMK AKTLGLAST TLSPREPPP SSANTETAG ISKLYVFIA AGVLGACGV LFAAIGLLL FCRKQSKQK KTGTRGDHD KACDDHHHI QADATEYVV SPKSGPRTN GCVAPNRIT ITANPLADA DNKNQNMIE MSCLSSQNN NCSTGQPSV SGEDSPTNS RDKKNKNRE KRIRNKHNV ESNTAGENY V
- >TC007250 RA
atgatcggg tttatttac gtccgcggt atttatagg tcctctact gaggacgaa attattcag ttccgtgcc gaaataggc gtcacgaat gtggtgggc ttcacttgg ctcctaaga ccagaagca agtgaagaa gccagaaaa attattgca gatatcgaa gaaattcta caaagctta gaatacgtg caggccata gacaaacaa aagtttctt ttggagaag tgcagaata gatgaggca cgcattaaa ctggttgag gcgtgcact cggggccaa catgttaac gaaaattgg catgtagca aggaaacat cgtttaacg gccagcagg tttggcatg gtactatct gcttgcagt agacgaaga ttcccaccc tctttattt aaaaatttg gcggaaggc tattcgctt gacagggtt gctgctgtg cagtggggt aagacccac gagaagaca gcgctcaga gaatttgaa gaagcgacg aatcttaaa gtccaagag acgggattt tggttggaa gaatcaggc tttttggga gcaagtcct gatggatta gtggaagaa gatgggatt ctcgaaatt aaatgccca tataaatat agggacact gacagtttg tctgaagcc ctgaaggat aaaaaatat ttttattgg agggatgaa aatgaggac attaatctt aacagcaat cacaattat taccaccaa gtacagggg caaatgcac atcactggt cgaaaagtc attaagtta gctgatgtt tacaagccg aaattatca aactatacg gcactgtct aggtcctct actgaggac gaaattatt cagttccgt gccgaaata ggcgtcacg aatgtggtg ggcttcact tggctccta agaccagaa gcaagtgaa gaagccaga aaaattatt gcagatatc gaagaaatt ctacaaagc ttagaatac gtgcaggcc atagacaaa caaaagttt cttttggag aagtgcaga atagatgag gcacgcatt aaactggtt gaggcgtgc actcggggc caacatgtt aacgaaaat tggcatgta gcaaggaaa catcgttta acggccagc aggtttggc atggtacta tctgcttgc agtagacga agattccca ccctcttta tttaaaaat ttggcggaa ggctattcg cttgacagg gttgctgct gtgcagtgg ggtaagacc cacgagaag acagcgctc agagaattt gaagaagcg acgaatctt aaagtccaa gagacggga ttttggttg gaagaatca ggctttttg ggagcaagt cctgatgga ttagtggaa gaagatggg attctcgaa attaaatgc ccatataaa tatagggac actgacagt ttgtctgaa gccctgaag gataaaaaa tatttttat tggagggat gaaaatgag gacattaat cttaacagc aatcacaat tattaccac caaggatgc agcagcatg gacgggttt cgggtgatt tgcattctt gtcgctgtt ttggcaacc acgggacgg gcaacagcg gccgatcac gtgcgcatg gtgaccaaa gctgattta ataacagtc ccgcttaac ttcgatacc agcattgta tgcacgatg aatatagtt ccggataat tttcaatgg aagttttat cctttgagt cggagacag gcctcgaat cccaaagct cacattagc ttaactcac tcgcatttc gaaattatc gatgagcgc aaatacgaa atcacccag aaaaagaca tcgacgctt aatcttcaa gccaaatcc gacgatatc gcgggggat taccaatgc ttggcttat tatggcgct tcggtcgtc gcctcaatc ccctggcgg ctgactata gccaatatc agcgaattt ccaccgcag gaaaaaatc gacgttact gtaattccc ggaaatacg gttaactgg aggtgcgag ccaccggaa gctaacccc gaaccttac atagactac cacaaaaac ggggtttac gtaccgccg ttcgtccaa gtcgttact aaatcgctg ataattcca aacgttact agtggtaat tcgggtagt tacacctgt aaggccggt aatgtttgc acggagaag ttctccccg gttttgttc aatttgaaa gttgataaa cgggcaccg agggaacca cccaagttt atagtccgt cccaggagt gtctacaat gtgactaaa ggagacaag gtcctgttg gaatgctcg gctgttggg aacccgctc ccgaaagtc gtttggcgc aaaataacc gggattatc ccggaaggt cgcagcgaa ttgacgcca ggaggtcta atcatcaaa aacatctcg gccagtgat aacggaatt tacgagtgc acccatgac aattccctt gggaaagtc gtacattcg atcactctg aactacaac gaggctccg accattaat tgcccgcaa gactcgatc gacgtcaat cagggcgaa gacctggag cttgaatgc acagttacc gggaacccc gacccggaa ataacgtgg ttcctgaac ggcttcgtc acaaaagac aataaaatc atcacagaa ggaaacaag atatttttc aaaccgact gaaaaaaga cacgcgggg aatttgcaa gtctttgcc cggaacatt gccggaacc gtctacagc agtatttcc atcaaagtc atccccttg tcaacaaca caagacaac gtgagcgaa aacccgctt ccaccaact cgcttacat cgccccaat tcgcgcaaa ttcaacaaa catgcaaaa atgtatcca ccgtccaaa cccaacatc actgggatc agtgacgag gcggtggtt ttgcgctgg acggtgcaa aataaggga ctcccgatc cagtttttc aaagtccag tataaggac ttcgggcct gcgaaccag cacgaccac cacaacaag ggcaagggc tcgaaatgg aacaccgat aaccccatc attgagccg cacatccga agttaccaa gtgaataac ttgaagccg gaccacgtg taccgattc cgaatcgcc gccgtttat agcaacagt gacaacaag aatagtccg aattccgac aagtttcat ttgaggaaa ttagacttt gacaagcgg aatccactg ccggttcct ctgatcacc aagactgaa agtatcaat acgacagcg gttaaaatc tactggaag tgtgagccg tcgattaat atcacaatt gatggcttt tttgtcaat tataactcc gctagtagt gcaggtgat tacacgaag gcgactgtc gatggcccc caaacgaat tccttcgtt ctgtcacat ctacaacct gataccgtt tacgatatt aagttgcag agtttcaac tcgaaatcg gcgagcgat tttagctcc ataatgaaa gccaagact cttggttta gcctcgacg acgctctcg cctcgagaa cctcccccg tcgtcggca aacaccgaa actgcggga attagcaaa ttgtacgta tttatagca gcgggagtg ttgggcgct tgcggcgtg ttgttcgcc gcaatcgga ttgttatta ttctgccgg aagcaatcc aagcagaaa aaaactggc acaagaggc gatcatgat aaagcctgt gatgaccac caccacatc caagccgac gctactgag tacgtggtg agtccgaaa agcggtccc cggaccaac ggctgtgtg gcccccaac cggatcaca atcaccgca aacccacta gcggacgcc gacaacaag aaatttcta gctcggatt ttttccaga atcagaata tga
- >TC007250 RB
atgatcggg tttatttac gtccgcggt atttatagg tcctctact gaggacgaa attattcag ttccgtgcc gaaataggc gtcacgaat gtggtgggc ttcacttgg ctcctaaga ccagaagca agtgaagaa gccagaaaa attattgca gatatcgaa gaaattcta caaagctta gaatacgtg caggccata gacaaacaa aagtttctt ttggagaag tgcagaata gatgaggca cgcattaaa ctggttgag gcgtgcact cggggccaa catgttaac gaaaattgg catgtagca aggaaacat cgtttaacg gccagcagg tttggcatg gtactatct gcttgcagt agacgaaga ttcccaccc tctttattt aaaaatttg gcggaaggc tattcgctt gacagggtt gctgctgtg cagtggggt aagacccac gagaagaca gcgctcaga gaatttgaa gaagcgacg aatcttaaa gtccaagag acgggattt tggttggaa gaatcaggc tttttggga gcaagtcct gatggatta gtggaagaa gatgggatt ctcgaaatt aaatgccca tataaatat agggacact gacagtttg tctgaagcc ctgaaggat aaaaaatat ttttattgg agggatgaa aatgaggac attaatctt aacagcaat cacaattat taccaccaa gtacagggg caaatgcac atcactggt cgaaaagtc attaagtta gctgatgtt tacaagccg aaattatca aactatacg gcactgtct aggtcctct actgaggac gaaattatt cagttccgt gccgaaata ggcgtcacg aatgtggtg ggcttcact tggctccta agaccagaa gcaagtgaa gaagccaga aaaattatt gcagatatc gaagaaatt ctacaaagc ttagaatac gtgcaggcc atagacaaa caaaagttt cttttggag aagtgcaga atagatgag gcacgcatt aaactggtt gaggcgtgc actcggggc caacatgtt aacgaaaat tggcatgta gcaaggaaa catcgttta acggccagc aggtttggc atggtacta tctgcttgc agtagacga agattccca ccctcttta tttaaaaat ttggcggaa ggctattcg cttgacagg gttgctgct gtgcagtgg ggtaagacc cacgagaag acagcgctc agagaattt gaagaagcg acgaatctt aaagtccaa gagacggga ttttggttg gaagaatca ggctttttg ggagcaagt cctgatgga ttagtggaa gaagatggg attctcgaa attaaatgc ccatataaa tatagggac actgacagt ttgtctgaa gccctgaag gataaaaaa tatttttat tggagggat gaaaatgag gacattaat cttaacagc aatcacaat tattaccac caaggatgc agcagcatg gacgggttt cgggtgatt tgcattctt gtcgctgtt ttggcaacc acgggacgg gcaacagcg gccgatcac gtgcgcatg gtgaccaaa gctgattta ataacagtc ccgcttaac ttcgatacc agcattgta tgcacgatg aatatagtt ccggataat tttcaatgg aagttttat cctttgagt cggagacag gcctcgaat cccaaagct cacattagc ttaactcac tcgcatttc gaaattatc gatgagcgc aaatacgaa atcacccag aaaaagaca tcgacgctt aatcttcaa gccaaatcc gacgatatc gcgggggat taccaatgc ttggcttat tatggcgct tcggtcgtc gcctcaatc ccctggcgg ctgactata gccaatatc agcgaattt ccaccgcag gaaaaaatc gacgttact gtaattccc ggaaatacg gttaactgg aggtgcgag ccaccggaa gctaacccc gaaccttac atagactac cacaaaaac ggggtttac gtaccgccg ttcgtccaa gtcgttact aaatcgctg ataattcca aacgttact agtggtaat tcgggtagt tacacctgt aaggccggt aatgtttgc acggagaag ttctccccg gttttgttc aatttgaaa gttgataaa cgggcaccg agggaacca cccaagttt atagtccgt cccaggagt gtctacaat gtgactaaa ggagacaag gtcctgttg gaatgctcg gctgttggg aacccgctc ccgaaagtc gtttggcgc aaaataacc gggattatc ccggaaggt cgcagcgaa ttgacgcca ggaggtcta atcatcaaa aacatctcg gccagtgat aacggaatt tacgagtgc acccatgac aattccctt gggaaagtc gtacattcg atcactctg aactacaac gaggctccg accattaat tgcccgcaa gactcgatc gacgtcaat cagggcgaa gacctggag cttgaatgc acagttacc gggaacccc gacccggaa ataacgtgg ttcctgaac ggcttcgtc acaaaagac aataaaatc atcacagaa ggaaacaag atatttttc aaaccgact gaaaaaaga cacgcgggg aatttgcaa gtctttgcc cggaacatt gccggaacc gtctacagc agtatttcc atcaaagtc atccccttg tcaacaaca caagacaac gtgagcgaa aacccgctt ccaccaact cgcttacat cgccccaat tcgcgcaaa ttcaacaaa catgcaaaa atgtatcca ccgtccaaa cccaacatc actgggatc agtgacgag gcggtggtt ttgcgctgg acggtgcaa aataaggga ctcccgatc cagtttttc aaagtccag tataaggac ttcgggcct gcgaaccag cacgaccac cacaacaag ggcaagggc tcgaaatgg aacaccgat aaccccatc attgagccg cacatccga agttaccaa gtgaataac ttgaagccg gaccacgtg taccgattc cgaatcgcc gccgtttat agcaacagt gacaacaag aatagtccg aattccgac aagtttcat ttgaggaaa ttagacttt gacaagcgg aatccactg ccggttcct ctgatcacc aagactgaa agtatcaat acgacagcg gttaaaatc tactggaag tgtgagccg tcgattaat atcacaatt gatggcttt tttgtcaat tataactcc gctagtagt gcaggtgat tacacgaag gcgactgtc gatggcccc caaacgaat tccttcgtt ctgtcacat ctacaacct gataccgtt tacgatatt aagttgcag agtttcaac tcgaaatcg gcgagcgat tttagctcc ataatgaaa gccaagact cttggttta gcctcgacg acgctctcg cctcgagaa cctcccccg tcgtcggca aacaccgaa actgcggga attagcaaa ttgtacgta tttatagca gcgggagtg ttgggcgct tgcggcgtg ttgttcgcc gcaatcgga ttgttatta ttctgccgg aagcaatcc aagcagaaa aaaactggc acaagaggc gatcatgat aaagcctgt gatgaccac caccacatc caagccgac gctactgag tacgtggtg agtccgaaa agcggtccc cggaccaac ggctgtgtg gcccccaac cggatcaca atcaccgca aacccacta gcggacgcc gacaacaag aatcagaat atgattgaa atgtcctgt ttgtcgtcg caaaacaac aactgctcg actggccaa ccgtcggtc agtggagag gactcaccg acgaactcc agagataaa aaaaataaa aatagggaa aaacgtatt agaaataag cacaacgtt gagtctaac acggccggc gaaaactat gtgtag
- >TC007250 RA
tatgcaaca cagttcgct ccttgtccg tctctttcc cggcatggt cgtccctgt ctagcccat ttcacctgc catctcgga tggaccaaa gaaactggt cgtcttcga ccggaatca acacaaaac acaagttgt gtttgcttc ggacgcttc acaagttgc gcgctcgcc gtaaactgt gttcctaag ccaattaaa cagcaacaa gcttttaaa aacgcttcc aataactta aaagtgtcc cgaaaaata atgtagagt gtagatgtg ttaaaaaag ccccgaaac tccccattt cggcgaaat accaccgca tttcagttc caaagagac ggacaagat gactgattg agaacgcta caaatttcc cttggataa ggtacgttc agatttata ttgtcacga aatctttta cacgctcat ttatctcct ttccatctc gtaatgatc gggtttatt tacgtccgc ggtatttat aggtcctct actgaggac gaaattatt cagttccgt gccgaaata ggcgtcacg aatgtggtg ggcttcact tggctccta agaccagaa gcaagtgaa gaagccaga aaaattatt gcagatatc gaagaaatt ctacaaagc ttagaatac gtgcaggcc atagacaaa caaaagttt cttttggag aagtgcaga atagatgag gcacgcatt aaactggtt gaggcgtgc actcggggc caacatgtt aacgaaaat tggcatgta gcaaggaaa catcgttta acggccagc aggtttggc atggtacta tctgcttgc agtagacga agattccca ccctcttta tttaaaaat ttggcggaa ggctattcg cttgacagg gttgctgct gtgcagtgg ggtaagacc cacgagaag acagcgctc agagaattt gaagaagcg acgaatctt aaagtccaa gagacggga ttttggttg gaagaatca ggctttttg ggagcaagt cctgatgga ttagtggaa gaagatggg attctcgaa attaaatgc ccatataaa tatagggac actgacagt ttgtctgaa gccctgaag gataaaaaa tatttttat tggagggat gaaaatgag gacattaat cttaacagc aatcacaat tattaccac caagtacag gggcaaatg cacatcact ggtcgaaaa gtcattaag ttagctgat gtttacaag ccgaaatta tcaaactat acggcactg tctaggtcc tctactgag gacgaaatt attcagttc cgtgccgaa ataggcgtc acgaatgtg gtgggcttc acttggctc ctaagacca gaagcaagt gaagaagcc agaaaaatt attgcagat atcgaagaa attctacaa agcttagaa tacgtgcag gccatagac aaacaaaag tttcttttg gagaagtgc agaatagat gaggcacgc attaaactg gttgaggcg tgcactcgg ggccaacat gttaacgaa aattggcat gtagcaagg aaacatcgt ttaacggcc agcaggttt ggcatggta ctatctgct tgcagtaga cgaagattc ccaccctct ttatttaaa aatttggcg gaaggctat tcgcttgac agggttgct gctgtgcag tggggtaag acccacgag aagacagcg ctcagagaa tttgaagaa gcgacgaat cttaaagtc caagagacg ggattttgg ttggaagaa tcaggcttt ttgggagca agtcctgat ggattagtg gaagaagat gggattctc gaaattaaa tgcccatat aaatatagg gacactgac agtttgtct gaagccctg aaggataaa aaatatttt tattggagg gatgaaaat gaggacatt aatcttaac agcaatcac aattattac caccaagga tgcagcagc atggacggg tttcgggtg atttgcatt cttgtcgct gttttggca accacggga cgggcaaca gcggccgat cacgtgcgc atggtgacc aaagctgat ttaataaca gtcccgctt aacttcgat accagcatt gtatgcacg atgaatata gttccggat aattttcaa tggaagttt tatcctttg agtcggaga caggcctcg aatcccaaa gctcacatt agcttaact cactcgcat ttcgaaatt atcgatgag cgcaaatac gaaatcacc cagaaaaag acatcgacg cttaatctt caagccaaa tccgacgat atcgcgggg gattaccaa tgcttggct tattatggc gcttcggtc gtcgcctca atcccctgg cggctgact atagccaat atcagcgaa tttccaccg caggaaaaa atcgacgtt actgtaatt cccggaaat acggttaac tggaggtgc gagccaccg gaagctaac cccgaacct tacatagac taccacaaa aacggggtt tacgtaccg ccgttcgtc caagtcgtt actaaatcg ctgataatt ccaaacgtt actagtggt aattcgggt agttacacc tgtaaggcc ggtaatgtt tgcacggag aagttctcc ccggttttg ttcaatttg aaagttgat aaacgggca ccgagggaa ccacccaag tttatagtc cgtcccagg agtgtctac aatgtgact aaaggagac aaggtcctg ttggaatgc tcggctgtt gggaacccg ctcccgaaa gtcgtttgg cgcaaaata accgggatt atcccggaa ggtcgcagc gaattgacg ccaggaggt ctaatcatc aaaaacatc tcggccagt gataacgga atttacgag tgcacccat gacaattcc cttgggaaa gtcgtacat tcgatcact ctgaactac aacgaggct ccgaccatt aattgcccg caagactcg atcgacgtc aatcagggc gaagacctg gagcttgaa tgcacagtt accgggaac cccgacccg gaaataacg tggttcctg aacggcttc gtcacaaaa gacaataaa atcatcaca gaaggaaac aagatattt ttcaaaccg actgaaaaa agacacgcg gggaatttg caagtcttt gcccggaac attgccgga accgtctac agcagtatt tccatcaaa gtcatcccc ttgtcaaca acacaagac aacgtgagc gaaaacccg cttccacca actcgctta catcgcccc aattcgcgc aaattcaac aaacatgca aaaatgtat ccaccgtcc aaacccaac atcactggg atcagtgac gaggcggtg gttttgcgc tggacggtg caaaataag ggactcccg atccagttt ttcaaagtc cagtataag gacttcggg cctgcgaac cagcacgac caccacaac aagggcaag ggctcgaaa tggaacacc gataacccc atcattgag ccgcacatc cgaagttac caagtgaat aacttgaag ccggaccac gtgtaccga ttccgaatc gccgccgtt tatagcaac agtgacaac aagaatagt ccgaattcc gacaagttt catttgagg aaattagac tttgacaag cggaatcca ctgccggtt cctctgatc accaagact gaaagtatc aatacgaca gcggttaaa atctactgg aagtgtgag ccgtcgatt aatatcaca attgatggc ttttttgtc aattataac tccgctagt agtgcaggt gattacacg aaggcgact gtcgatggc ccccaaacg aattccttc gttctgtca catctacaa cctgatacc gtttacgat attaagttg cagagtttc aactcgaaa tcggcgagc gattttagc tccataatg aaagccaag actcttggt ttagcctcg acgacgctc tcgcctcga gaacctccc ccgtcgtcg gcaaacacc gaaactgcg ggaattagc aaattgtac gtatttata gcagcggga gtgttgggc gcttgcggc gtgttgttc gccgcaatc ggattgtta ttattctgc cggaagcaa tccaagcag aaaaaaact ggcacaaga ggcgatcat gataaagcc tgtgatgac caccaccac atccaagcc gacgctact gagtacgtg gtgagtccg aaaagcggt ccccggacc aacggctgt gtggccccc aaccggatc acaatcacc gcaaaccca ctagcggac gccgacaac aagaaattt ctagctcgg attttttcc agaatcaga atatgattg aaatgtcct gtttgtcgt cgcaaaaca acaactgct cgactggcc aaccgtcgg tcagtggag aggactcac cgacgaact ccagagata aaaaaaata aaaataggg aaaaacgta ttagaaata agcacaacg ttgagtcta acacggccg gcgaaaact atgtgtagt gaaatttcg tgacttttt aatgcctta aataagact gatacgaag tgccttcgc aaaaaatag gtaattagt ttttgataa cttgcctca ctgtatata gaaattttt gtacatatt tttattcga cgaaagaag aatgtcatt cgagtgatc tgttttaat tatttaact tattttttg tacttatta attgctaag tagccgatt aaatgatta tttattaat ttccagtat taataacaa actttggca gctaacgcg tattcgtag agatactaa aattggcac acattaagc atccgataa cattaaaag acaagaata acattcatt aactaatta ggcgtgtga tgtgtgcca tgaaaacaa tccgttaac gacaccccg ggtcacgat aaaagacca gttcatatg atttataat aagagctac caaatcaaa tacgaactt tgtaaatat ttagataga agcatcctt tgattgccc tacagcttg ccaagtcac acttatctt gtaatagta caaatgcag atttctctt cgctatttt ttaccttca gcttttata aaaataggt tgtatttca acgaataaa taatcatca ttgactctc attttttcc ctcttccca tggaaacct cggacgaga atttggttt agtcaagtg aacaattgg attttttgc ccatctcta gccgaggga taatatgct gagttgtaa ttttttctt gtattgtca tgacgccaa tcaagaatg taacgtcct ggaattatc aaaaactat ccaataaag aatgcccaa gtaa
- >TC007250 RB
tatgcaaca cagttcgct ccttgtccg tctctttcc cggcatggt cgtccctgt ctagcccat ttcacctgc catctcgga tggaccaaa gaaactggt cgtcttcga ccggaatca acacaaaac acaagttgt gtttgcttc ggacgcttc acaagttgc gcgctcgcc gtaaactgt gttcctaag ccaattaaa cagcaacaa gcttttaaa aacgcttcc aataactta aaagtgtcc cgaaaaata atgtagagt gtagatgtg ttaaaaaag ccccgaaac tccccattt cggcgaaat accaccgca tttcagttc caaagagac ggacaagat gactgattg agaacgcta caaatttcc cttggataa ggtacgttc agatttata ttgtcacga aatctttta cacgctcat ttatctcct ttccatctc gtaatgatc gggtttatt tacgtccgc ggtatttat aggtcctct actgaggac gaaattatt cagttccgt gccgaaata ggcgtcacg aatgtggtg ggcttcact tggctccta agaccagaa gcaagtgaa gaagccaga aaaattatt gcagatatc gaagaaatt ctacaaagc ttagaatac gtgcaggcc atagacaaa caaaagttt cttttggag aagtgcaga atagatgag gcacgcatt aaactggtt gaggcgtgc actcggggc caacatgtt aacgaaaat tggcatgta gcaaggaaa catcgttta acggccagc aggtttggc atggtacta tctgcttgc agtagacga agattccca ccctcttta tttaaaaat ttggcggaa ggctattcg cttgacagg gttgctgct gtgcagtgg ggtaagacc cacgagaag acagcgctc agagaattt gaagaagcg acgaatctt aaagtccaa gagacggga ttttggttg gaagaatca ggctttttg ggagcaagt cctgatgga ttagtggaa gaagatggg attctcgaa attaaatgc ccatataaa tatagggac actgacagt ttgtctgaa gccctgaag gataaaaaa tatttttat tggagggat gaaaatgag gacattaat cttaacagc aatcacaat tattaccac caagtacag gggcaaatg cacatcact ggtcgaaaa gtcattaag ttagctgat gtttacaag ccgaaatta tcaaactat acggcactg tctaggtcc tctactgag gacgaaatt attcagttc cgtgccgaa ataggcgtc acgaatgtg gtgggcttc acttggctc ctaagacca gaagcaagt gaagaagcc agaaaaatt attgcagat atcgaagaa attctacaa agcttagaa tacgtgcag gccatagac aaacaaaag tttcttttg gagaagtgc agaatagat gaggcacgc attaaactg gttgaggcg tgcactcgg ggccaacat gttaacgaa aattggcat gtagcaagg aaacatcgt ttaacggcc agcaggttt ggcatggta ctatctgct tgcagtaga cgaagattc ccaccctct ttatttaaa aatttggcg gaaggctat tcgcttgac agggttgct gctgtgcag tggggtaag acccacgag aagacagcg ctcagagaa tttgaagaa gcgacgaat cttaaagtc caagagacg ggattttgg ttggaagaa tcaggcttt ttgggagca agtcctgat ggattagtg gaagaagat gggattctc gaaattaaa tgcccatat aaatatagg gacactgac agtttgtct gaagccctg aaggataaa aaatatttt tattggagg gatgaaaat gaggacatt aatcttaac agcaatcac aattattac caccaagga tgcagcagc atggacggg tttcgggtg atttgcatt cttgtcgct gttttggca accacggga cgggcaaca gcggccgat cacgtgcgc atggtgacc aaagctgat ttaataaca gtcccgctt aacttcgat accagcatt gtatgcacg atgaatata gttccggat aattttcaa tggaagttt tatcctttg agtcggaga caggcctcg aatcccaaa gctcacatt agcttaact cactcgcat ttcgaaatt atcgatgag cgcaaatac gaaatcacc cagaaaaag acatcgacg cttaatctt caagccaaa tccgacgat atcgcgggg gattaccaa tgcttggct tattatggc gcttcggtc gtcgcctca atcccctgg cggctgact atagccaat atcagcgaa tttccaccg caggaaaaa atcgacgtt actgtaatt cccggaaat acggttaac tggaggtgc gagccaccg gaagctaac cccgaacct tacatagac taccacaaa aacggggtt tacgtaccg ccgttcgtc caagtcgtt actaaatcg ctgataatt ccaaacgtt actagtggt aattcgggt agttacacc tgtaaggcc ggtaatgtt tgcacggag aagttctcc ccggttttg ttcaatttg aaagttgat aaacgggca ccgagggaa ccacccaag tttatagtc cgtcccagg agtgtctac aatgtgact aaaggagac aaggtcctg ttggaatgc tcggctgtt gggaacccg ctcccgaaa gtcgtttgg cgcaaaata accgggatt atcccggaa ggtcgcagc gaattgacg ccaggaggt ctaatcatc aaaaacatc tcggccagt gataacgga atttacgag tgcacccat gacaattcc cttgggaaa gtcgtacat tcgatcact ctgaactac aacgaggct ccgaccatt aattgcccg caagactcg atcgacgtc aatcagggc gaagacctg gagcttgaa tgcacagtt accgggaac cccgacccg gaaataacg tggttcctg aacggcttc gtcacaaaa gacaataaa atcatcaca gaaggaaac aagatattt ttcaaaccg actgaaaaa agacacgcg gggaatttg caagtcttt gcccggaac attgccgga accgtctac agcagtatt tccatcaaa gtcatcccc ttgtcaaca acacaagac aacgtgagc gaaaacccg cttccacca actcgctta catcgcccc aattcgcgc aaattcaac aaacatgca aaaatgtat ccaccgtcc aaacccaac atcactggg atcagtgac gaggcggtg gttttgcgc tggacggtg caaaataag ggactcccg atccagttt ttcaaagtc cagtataag gacttcggg cctgcgaac cagcacgac caccacaac aagggcaag ggctcgaaa tggaacacc gataacccc atcattgag ccgcacatc cgaagttac caagtgaat aacttgaag ccggaccac gtgtaccga ttccgaatc gccgccgtt tatagcaac agtgacaac aagaatagt ccgaattcc gacaagttt catttgagg aaattagac tttgacaag cggaatcca ctgccggtt cctctgatc accaagact gaaagtatc aatacgaca gcggttaaa atctactgg aagtgtgag ccgtcgatt aatatcaca attgatggc ttttttgtc aattataac tccgctagt agtgcaggt gattacacg aaggcgact gtcgatggc ccccaaacg aattccttc gttctgtca catctacaa cctgatacc gtttacgat attaagttg cagagtttc aactcgaaa tcggcgagc gattttagc tccataatg aaagccaag actcttggt ttagcctcg acgacgctc tcgcctcga gaacctccc ccgtcgtcg gcaaacacc gaaactgcg ggaattagc aaattgtac gtatttata gcagcggga gtgttgggc gcttgcggc gtgttgttc gccgcaatc ggattgtta ttattctgc cggaagcaa tccaagcag aaaaaaact ggcacaaga ggcgatcat gataaagcc tgtgatgac caccaccac atccaagcc gacgctact gagtacgtg gtgagtccg aaaagcggt ccccggacc aacggctgt gtggccccc aaccggatc acaatcacc gcaaaccca ctagcggac gccgacaac aagaatcag aatatgatt gaaatgtcc tgtttgtcg tcgcaaaac aacaactgc tcgactggc caaccgtcg gtcagtgga gaggactca ccgacgaac tccagagat aaaaaaaat aaaaatagg gaaaaacgt attagaaat aagcacaac gttgagtct aacacggcc ggcgaaaac tatgtgtag tgaaatttc gtgactttt taatgcctt aaataagac tgatacgaa gtgccttcg caaaaaata ggtaattag tttttgata acttgcctc actgtatat agaaatttt tgtacatat ttttattcg acgaaagaa gaatgtcat tcgagtgat ctgttttaa ttatttaac ttatttttt gtacttatt aattgctaa gtagccgat taaatgatt atttattaa tttccagta ttaataaca aactttggc agctaacgc gtattcgta gagatacta aaattggca cacattaag catccgata acattaaaa gacaagaat aacattcat taactaatt aggcgtgtg atgtgtgcc atgaaaaca atccgttaa cgacacccc gggtcacga taaaagacc agttcatat gatttataa taagagcta ccaaatcaa atacgaact ttgtaaata tttagatag aagcatcct ttgattgcc ctacagctt gccaagtca cacttatct tgtaatagt acaaatgca gatttctct tcgctattt tttaccttc agcttttat aaaaatagg ttgtatttc aacgaataa ataatcatc attgactct cattttttc cctcttccc atggaaacc tcggacgag aatttggtt tagtcaagt gaacaattg gattttttg cccatctct agccgaggg ataatatgc tgagttgta attttttct tgtattgtc atgacgcca atcaagaat gtaacgtcc tggaattat caaaaacta tccaataaa gaatgccca agtaa
The data is licensed under a
Creative Commons Attribution 3.0 Unported License. (CC-BY)
