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Gene information
General information
TC007234 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7112924..7114433
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007234
    iBeetle screen iB_04297

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    tropharium shape irregular - Number of animals showing the phenotype: 2

    tropharium size decreased - Number of animals showing the phenotype: 2
    previtellogenic egg chamber segmentation/division irregular - Number of animals showing the phenotype: 2
    mature egg not present - Number of animals showing the phenotype: 2
    previtellogenic egg chamber sometimes deposition present - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007234 RA
      MDKTDEEPT FQYFAVKND EETFVNISD MLDLPEVPD 
      DNSINKEIF YLAFDNNEL QEDSVPDDP LEGVGLDNM 
      NDSSTSFSE TDLKLITLG DGRIFVTTD NNLQSLQTQ 
      SMMVETPEE TETMTESAQ LSNEEESSV DNSDVNYQL 
      LQLEDGSQA IFTLYKIKN NEDVKDEPP KKVKKYQCP 
      HESCDKVYA TSYHLTVHI RSHTDCKPY PCSIEGCEK 
      KFATNYSLK AHIRTHTGE KPYACSVCT KQFKTSGDL 
      QKHLRIHTG EKPFECPIE GCGRSFTTS NIRKVHIRS 
      HTGERPYIC DCGKAFTSS TNYKNHLRI HSGEKPYVC 
      TVEGCGKRF TEYSSLYKH NAVHMPVRP HMCIFCGQR 
      FKQESALNL HKRLKHNVV VTKDGTEII VKIE
    CDS sequence
    • >TC007234 RA
      atggataag acggacgag gaaccgact tttcagtac 
      tttgctgtg aagaatgat gaggagact tttgttaac 
      atttccgat atgttggac ctgcctgaa gtaccagat 
      gataacagc ataaataag gaaatattt tatttggct 
      tttgacaac aatgaactg caggaggac tcggttcct 
      gacgacccc cttgaaggt gtgggcttg gataacatg 
      aatgattcg tccacttca ttcagtgaa acggattta 
      aagcttata acacttggc gatggcagg atatttgtt 
      actacagat aataacttg cagagctta caaacacaa 
      tctatgatg gtggaaacg cctgaagaa actgaaacg 
      atgactgaa tcggctcaa ctttccaac gaagaggaa 
      tcatcagtt gacaattct gatgtaaat taccaattg 
      ctccaactg gaggacggc tctcaggcc atttttacg 
      ttatataaa ataaaaaac aacgaagac gtgaaggac 
      gagcctcca aaaaaagta aaaaaatat caatgcccg 
      cacgagagt tgcgataag gtttacgcg acttcgtac 
      cacctcact gtgcacata aggagccac actgactgt 
      aagccttac ccgtgttcc atcgagggc tgtgagaaa 
      aaattcgcg actaattac tccctcaaa gcccacatt 
      cggacccac acgggcgaa aaaccatac gcgtgttcc 
      gtctgcact aagcaattt aaaacgtcg ggggattta 
      cagaaacat ctgagaatt cacactggt gaaaagccg 
      tttgaatgt ccaattgaa ggttgcggg agatcattc 
      acgacttcc aatatccgc aaagtccac atcaggtcg 
      cacaccggg gaaagaccg tacatttgc gattgtggg 
      aaagcattt acgtcgtca actaattac aagaatcat 
      ttgaggata cattccggg gaaaagccc tatgtttgt 
      acggtggaa ggctgcggg aaacgcttt accgagtat 
      tcgtctcta tataaacac aatgcggtt catatgcca 
      gtcaggccg cacatgtgc attttttgt ggacagagg 
      tttaagcaa gagtcggcg ttaaacttg cacaagagg 
      ctgaagcat aacgtggtt gtgactaaa gatgggact 
      gaaattatt gttaaaata gagtag
    mRNA sequence
    • >TC007234 RA
      tgagtgaac gcaaatgga ggactctct tcctcaggt 
      tcattatgg ataagacgg acgaggaac cgacttttc 
      agtactttg ctgtgaaga atgatgagg agacttttg 
      ttaacattt ccgatatgt tggacctgc ctgaagtac 
      cagatgata acagcataa ataaggaaa tattttatt 
      tggcttttg acaacaatg aactgcagg aggactcgg 
      ttcctgacg acccccttg aaggtgtgg gcttggata 
      acatgaatg attcgtcca cttcattca gtgaaacgg 
      atttaaagc ttataacac ttggcgatg gcaggatat 
      ttgttacta cagataata acttgcaga gcttacaaa 
      cacaatcta tgatggtgg aaacgcctg aagaaactg 
      aaacgatga ctgaatcgg ctcaacttt ccaacgaag 
      aggaatcat cagttgaca attctgatg taaattacc 
      aattgctcc aactggagg acggctctc aggccattt 
      ttacgttat ataaaataa aaaacaacg aagacgtga 
      aggacgagc ctccaaaaa aagtaaaaa aatatcaat 
      gcccgcacg agagttgcg ataaggttt acgcgactt 
      cgtaccacc tcactgtgc acataagga gccacactg 
      actgtaagc cttacccgt gttccatcg agggctgtg 
      agaaaaaat tcgcgacta attactccc tcaaagccc 
      acattcgga cccacacgg gcgaaaaac catacgcgt 
      gttccgtct gcactaagc aatttaaaa cgtcggggg 
      atttacaga aacatctga gaattcaca ctggtgaaa 
      agccgtttg aatgtccaa ttgaaggtt gcgggagat 
      cattcacga cttccaata tccgcaaag tccacatca 
      ggtcgcaca ccggggaaa gaccgtaca tttgcgatt 
      gtgggaaag catttacgt cgtcaacta attacaaga 
      atcatttga ggatacatt ccggggaaa agccctatg 
      tttgtacgg tggaaggct gcgggaaac gctttaccg 
      agtattcgt ctctatata aacacaatg cggttcata 
      tgccagtca ggccgcaca tgtgcattt tttgtggac 
      agaggttta agcaagagt cggcgttaa acttgcaca 
      agaggctga agcataacg tggttgtga ctaaagatg 
      ggactgaaa ttattgtta aaatagagt agttagaag 
      atttatttc tgttgcgaa acgtactat ttattatca 
      actagaaaa tagcggatt gtaccgata aataaattt 
      atgttatta ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY