Status
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Gene information
General information
TC007226 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7250657..7344619
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007226
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04296

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007226 RA
      MVDAPESGN FPDRIEPEV KNVALGCVL SRVEFRELK 
      VAWQVRRAN PLSRKKLYE LQHPQMDNE SAPNPIVSG 
      SWPAVDGVL GQSGRSRDG NRRRRQRRN AACTMIPPA 
      HTMPSLHFH LALIIGFCT QMIVADCPR LCECKWKSG 
      KESVSCPNA NLSSIPLHL EAGTQVLDV SKNNLVNLK 
      HDEFSKAGL LNLQKVYLS QCRLKNLER YAFRKLINL 
      VELDLSHNL LSSVPSHSF DSIPELREL KLNDNPIQR 
      ILNDAFINV PQLIRLELS ECRISTIEP RAFHGLESS 
      LEWLKLDYN KLTEVLSSS FTILENLHG LELAGNPWN 
      CTCPLRPLR LWMLQKNVP FGIPPICQS PKRLRSKTW 
      DKLDLDEFA CIPEIFAYE SKTKGVEGK NVTMTCRIT 
      GIPEPSVRW LLKNKVIAN LSGSSYSNG KKLYMVHLS 
      NNSSDLTIF SADLQDAGV YVCAAENKA GRAEASVTL 
      AVIRKPPET AFNNKILIA SVITGIALV LASCLIALC 
      VYSVRKKQM LTWRTRECR REDNYEKIE MNHKVAGNS 
      NGGAVQAEI AVVATKKNG EYRVVPVAD NEEVEEEEE 
      SNVEAAAKR IWQDTAIEK RWNSPEHLL DPEDLHIPR 
      RTLQEARDD IRKGFSSTS GIYSGYEQP STSLSQSVA 
      PHLLRRQIS GGHATTYNS ELSSLMEDE ADKKYPDLI 
      ENSSYKFGN KANSEGGSV ADITDLFCT LPRKRTLIQ 
      SARYKSSDS QSPLLPESR YGSSGGESS CGSQESGLR 
      RLSDCPKYP AGNLNKNRV NKISNSYLN LTREEELTS 
      TPLLDVTGL ESRVGLTPN ANSYDYHAA QLERFLEEY 
      RSLQKQLTK MKETCDNLC QDHLQAGSS QVTPSTSND 
      DVVRNNPVS PNSNQSLED SIDFRNFES ELTKYLLAK 
      SSPSPKTFT NNSGVFNN
    CDS sequence
    • >TC007226 RA
      atggtggat gcgccggaa tccggtaat ttcccggac 
      aggatcgaa cccgaggta aaaaatgtg gcgctgggc 
      tgtgtccta agtcgagtg gagtttagg gaattaaaa 
      gtggcctgg caagtccgg cgtgctaat cccttatcg 
      cgaaagaag ctttacgag ctgcagcat cctcagatg 
      gacaacgag tccgctcct aatccaatt gtatccggc 
      agttggccc gccgtggat ggggtgctg gggcagtca 
      ggccgtagc cgcgatgga aaccgaaga agaagacag 
      cgaagaaac gcagcctgt acgatgatc cccccagct 
      cacaccatg ccatctctc catttccac ctggccctc 
      atcatcggc ttttgcacc caaatgatc gtggcggac 
      tgtccgcgt ttatgcgag tgcaagtgg aagagtggc 
      aaggagtcg gtttcttgt cccaacgca aacttatct 
      tccattcct ctccatctg gaggccggc actcaagtc 
      ctcgacgtc tccaagaac aatctagtc aatttgaaa 
      cacgacgag ttcagcaaa gccggtctg ctcaacttg 
      cagaaagtg tacttgtct cagtgtcgt ctgaagaac 
      ttggaacgg tacgctttt aggaaacta atcaatttg 
      gtcgagttg gacctgagc cacaacctc ttgagcagt 
      gtcccctca cactcgttt gactcgatc cccgaactg 
      cgggaactc aagctcaac gataatccc atacaaaga 
      atactcaac gatgctttc atcaatgtt ccacaattg 
      atacggttg gagctatca gagtgtcgc atttcaaca 
      atcgaaccc cgagccttc cacggtcta gaatcttca 
      ctcgaatgg cttaagctc gactacaac aaactaacc 
      gaagtgctg tcttcgtct tttacgata ctagagaac 
      ttgcacggc ctggaactg gccggaaac ccttggaac 
      tgcacgtgt cctctccgc cctctccgc ttgtggatg 
      ttgcagaaa aacgtccct ttcgggatc ccccccatc 
      tgtcaaagc cccaaacgc ctccgctcc aagacctgg 
      gacaagcta gacctcgac gaattcgcc tgtataccc 
      gaaattttt gcttatgaa agcaaaact aaaggagtc 
      gaaggcaaa aacgtcacc atgacttgc cgaatcacc 
      ggaatcccg gaaccctca gtccggtgg ctgctcaag 
      aacaaagtg atcgccaac ctttcgggt tcctcttac 
      tccaacggt aaaaaactg tacatggtg cacttgtcg 
      aacaactcg agtgattta acaattttc agtgccgac 
      ctgcaagat gcaggtgtg tatgtgtgt gcggcggaa 
      aacaaggcg ggtcgtgcc gaagctagt gtaacatta 
      gccgtgatt aggaaaccg cctgaaacc gctttcaat 
      aacaaaata ttgattgcg agtgttatc acgggcatc 
      gcgttggtt ctagcctcg tgcttgatc gcactctgt 
      gtgtactcg gtgcggaag aagcaaatg ctcacatgg 
      cggactcgc gagtgcaga agggaggac aactatgag 
      aaaatcgaa atgaatcat aaagtggcg gggaattcc 
      aacggggga gcggtgcaa gctgagatc gcggtggtg 
      gcaacgaag aagaatggg gagtatagg gttgtccca 
      gtggcggat aatgaagag gttgaggag gaggaggag 
      agcaatgtt gaagcggcg gcaaagagg atatggcaa 
      gatactgcc attgaaaag cggtggaac tcgccggaa 
      catctcctg gatccggag gatctgcac ataccgagg 
      aggactcta caagaggca agagacgat atcaggaag 
      gggttcagt tcgacgtct ggtatatat agtgggtac 
      gagcagccg tcgacatcc ctaagccaa agcgtggca 
      ccgcatctc ctacgcagg caaatatcc ggaggccac 
      gcgacaacc tacaacagc gagctgtcg agtctgatg 
      gaggacgag gctgacaag aaatacccg gatttgatt 
      gagaactct tcgtacaag ttcgggaac aaggcgaac 
      agcgagggc ggcagcgtg gccgacatc acggacctc 
      ttctgcact ctgccccgg aaacgcacc ctcatacag 
      agcgcgcgg tacaagagc agcgacagc cagtcgccc 
      ctcctcccg gagagcagg tacggcagc agcggcggc 
      gaaagcagc tgcggcagc caggagtcc gggctccgg 
      cggctctcc gactgcccc aagtacccg gcagggaac 
      ctcaacaaa aacagggta aataaaatc tccaacagc 
      tacttgaac ctgacgcgg gaggaggag ctgacctcg 
      acgccgctg ctggacgtg acggggctg gaaagcagg 
      gtgggtctc accccgaat gcaaattcc tacgattac 
      catgcggcc cagctggag cgctttctg gaggagtac 
      cgcagcctc cagaagcag ctgacgaaa atgaaggaa 
      acttgcgac aacctgtgc caggaccat ctgcaggcg 
      gggtcctcg caggtgacc ccttccacg tcgaacgac 
      gatgttgtg aggaataat ccggtgtcg ccgaattca 
      aatcagtca ctcgaggac tcgattgat tttcgcaat 
      ttcgagagc gaactcacc aagtatttg ctcgccaaa 
      agttcaccc tcacccaaa actttcacc aacaacagc 
      ggagtgttc aataattga
    mRNA sequence
    • >TC007226 RA
      tgactttat tttaattgt gaccgagtc cggattaaa 
      ccgatttca ggtgcagaa aaggattca aagattttg 
      gacaaattt tgattaagg taatggttg tagctatgc 
      attttggtt caactatct gaaccaaaa aatggtgga 
      tgcgccgga atccggtaa tttcccgga caggatcga 
      acccgaggt aaaaaatgt ggcgctggg ctgtgtcct 
      aagtcgagt ggagtttag ggaattaaa agtggcctg 
      gcaagtccg gcgtgctaa tcccttatc gcgaaagaa 
      gctttacga gctgcagca tcctcagat ggacaacga 
      gtccgctcc taatccaat tgtatccgg cagttggcc 
      cgccgtgga tggggtgct ggggcagtc aggccgtag 
      ccgcgatgg aaaccgaag aagaagaca gcgaagaaa 
      cgcagcctg tacgatgat ccccccagc tcacaccat 
      gccatctct ccatttcca cctggccct catcatcgg 
      cttttgcac ccaaatgat cgtggcgga ctgtccgcg 
      tttatgcga gtgcaagtg gaagagtgg caaggagtc 
      ggtttcttg tcccaacgc aaacttatc ttccattcc 
      tctccatct ggaggccgg cactcaagt cctcgacgt 
      ctccaagaa caatctagt caatttgaa acacgacga 
      gttcagcaa agccggtct gctcaactt gcagaaagt 
      gtacttgtc tcagtgtcg tctgaagaa cttggaacg 
      gtacgcttt taggaaact aatcaattt ggtcgagtt 
      ggacctgag ccacaacct cttgagcag tgtcccctc 
      acactcgtt tgactcgat ccccgaact gcgggaact 
      caagctcaa cgataatcc catacaaag aatactcaa 
      cgatgcttt catcaatgt tccacaatt gatacggtt 
      ggagctatc agagtgtcg catttcaac aatcgaacc 
      ccgagcctt ccacggtct agaatcttc actcgaatg 
      gcttaagct cgactacaa caaactaac cgaagtgct 
      gtcttcgtc ttttacgat actagagaa cttgcacgg 
      cctggaact ggccggaaa cccttggaa ctgcacgtg 
      tcctctccg ccctctccg cttgtggat gttgcagaa 
      aaacgtccc tttcgggat cccccccat ctgtcaaag 
      ccccaaacg cctccgctc caagacctg ggacaagct 
      agacctcga cgaattcgc ctgtatacc cgaaatttt 
      tgcttatga aagcaaaac taaaggagt cgaaggcaa 
      aaacgtcac catgacttg ccgaatcac cggaatccc 
      ggaaccctc agtccggtg gctgctcaa gaacaaagt 
      gatcgccaa cctttcggg ttcctctta ctccaacgg 
      taaaaaact gtacatggt gcacttgtc gaacaactc 
      gagtgattt aacaatttt cagtgccga cctgcaaga 
      tgcaggtgt gtatgtgtg tgcggcgga aaacaaggc 
      gggtcgtgc cgaagctag tgtaacatt agccgtgat 
      taggaaacc gcctgaaac cgctttcaa taacaaaat 
      attgattgc gagtgttat cacgggcat cgcgttggt 
      tctagcctc gtgcttgat cgcactctg tgtgtactc 
      ggtgcggaa gaagcaaat gctcacatg gcggactcg 
      cgagtgcag aagggagga caactatga gaaaatcga 
      aatgaatca taaagtggc ggggaattc caacggggg 
      agcggtgca agctgagat cgcggtggt ggcaacgaa 
      gaagaatgg ggagtatag ggttgtccc agtggcgga 
      taatgaaga ggttgagga ggaggagga gagcaatgt 
      tgaagcggc ggcaaagag gatatggca agatactgc 
      cattgaaaa gcggtggaa ctcgccgga acatctcct 
      ggatccgga ggatctgca cataccgag gaggactct 
      acaagaggc aagagacga tatcaggaa ggggttcag 
      ttcgacgtc tggtatata tagtgggta cgagcagcc 
      gtcgacatc cctaagcca aagcgtggc accgcatct 
      cctacgcag gcaaatatc cggaggcca cgcgacaac 
      ctacaacag cgagctgtc gagtctgat ggaggacga 
      ggctgacaa gaaataccc ggatttgat tgagaactc 
      ttcgtacaa gttcgggaa caaggcgaa cagcgaggg 
      cggcagcgt ggccgacat cacggacct cttctgcac 
      tctgccccg gaaacgcac cctcataca gagcgcgcg 
      gtacaagag cagcgacag ccagtcgcc cctcctccc 
      ggagagcag gtacggcag cagcggcgg cgaaagcag 
      ctgcggcag ccaggagtc cgggctccg gcggctctc 
      cgactgccc caagtaccc ggcagggaa cctcaacaa 
      aaacagggt aaataaaat ctccaacag ctacttgaa 
      cctgacgcg ggaggagga gctgacctc gacgccgct 
      gctggacgt gacggggct ggaaagcag ggtgggtct 
      caccccgaa tgcaaattc ctacgatta ccatgcggc 
      ccagctgga gcgctttct ggaggagta ccgcagcct 
      ccagaagca gctgacgaa aatgaagga aacttgcga 
      caacctgtg ccaggacca tctgcaggc ggggtcctc 
      gcaggtgac cccttccac gtcgaacga cgatgttgt 
      gaggaataa tccggtgtc gccgaattc aaatcagtc 
      actcgagga ctcgattga ttttcgcaa tttcgagag 
      cgaactcac caagtattt gctcgccaa aagttcacc 
      ctcacccaa aactttcac caacaacag cggagtgtt 
      caataattg aatttgacg acaaaaaat ttgtgtcac 
      agtgataat tacgatggc aattgcccg gagtgacgc 
      tacacaagg atttaaacg actcccaga aatttttac 
      taatgtgta taaattaac attgtacat acctggatg 
      actgttacg tatcatttg tttgttaaa tgttgatag 
      ttctagcag acatttgtg atattataa aggtagcat 
      tttatttat cgacttgat gggtttttc atatcgtac 
      gaattacgt tctactgta ataacacat atcctttat 
      aagtttgta aattattta aataaatgc attctattt 
      at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY