Status
Please wait ...
Gene information
General information
TC007197 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7679221..7749832
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007197
    iBeetle screen iB_04292

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007197 RA
      MLLYIVAAA ILQILLQTT VTSAVSWEE WWTYDGISG 
      PAFWGLINP EWSLCNKGR RQSPVNLEP NKLLFDPNL 
      RPLHIDKHR VSGSIMNTG HSVIFTVDN DTRHHINVT 
      GGPLSYKYQ FQEIHIHYG LHDQFGSEH SINGYNFPA 
      EIQIFGFNS QLYTNFSEA LHKAQGIVV ISLLLQMGD 
      LSNPELRIL TDQLEKIRY GGDEVEVKR LSVRGLLPE 
      TDYYMTYDG STTMPACHE TVTWLILNK PIYITKQQM 
      HALRRLMQG DAKHPKAPL GNNFRPPQP LHHRPVRTN 
      IDFSVKHRT DAGKQCPSM YKDVYYKAN SWKQH
    CDS sequence
    • >TC007197 RA
      atgttgctc tacattgtt gcagcagca atcctgcaa 
      atcctgcta caaaccaca gtgacgtct gctgtgagc 
      tgggaggaa tggtggact tatgatgga atttcgggt 
      ccggcgttt tggggcctg ataaatcca gaatggtca 
      ctgtgcaac aagggacgg aggcagtct ccggttaat 
      ctagagccg aataagctg ctcttcgac ccaaacctt 
      cgacccctt cacatcgat aaacacagg gtgagcggt 
      tccatcatg aatacgggc catagtgta atatttaca 
      gtagataac gacactcgg catcacata aatgtgact 
      gggggtcca ctatcctat aaatatcag tttcaagaa 
      atccatatt cactacgga ctccacgat cagtttggg 
      tccgaacat tcaataaat ggatacaac ttccctgca 
      gagatacag attttcggg ttcaactcg caactctac 
      accaacttc tcggaagcg ctccacaaa gctcaaggc 
      atcgtggtt atttctctt ctcttacag atgggtgac 
      ctttccaat cccgagctg aggatatta acagatcag 
      ctagaaaaa attagatac ggcggggac gaggtcgag 
      gtgaaacgc ctttcagtg cgaggtcta cttcctgaa 
      accgactat tacatgacg tacgatgga tccacgacg 
      atgccggcc tgccacgag accgtcaca tggttgatc 
      ctcaacaag cctatttac atcaccaag caacagatg 
      catgcgtta cgcagactg atgcaagga gatgccaaa 
      caccccaaa gccccgttg ggaaacaac ttccgacca 
      ccacagccc ctgcaccat cgaccagtt cggactaat 
      attgacttc agcgttaaa caccgcact gacgcggga 
      aagcaatgt cccagcatg tacaaagac gtctactat 
      aaggcaaac agttggaaa cagcactga
    mRNA sequence
    • >TC007197 RA
      cacatggtg ttcggatgc ggcagagtg cggtgcggt 
      gcttcggta aaaacgcgc tcattcttc acgtaccag 
      agataaatt gcggccaaa aacagacat gctcgagtg 
      ccggagaag ttgcaaaat acaaatcgt gtgatgagt 
      caaaataag aataagaga aacttatac acaagaatg 
      aaactagcg tgacactca aattattca acatgttgc 
      tctacattg ttgcagcag caatcctgc aaatcctgc 
      tacaaacca cagtgacgt ctgctgtga gctgggagg 
      aatggtgga cttatgatg gaatttcgg gtccggcgt 
      tttggggcc tgataaatc cagaatggt cactgtgca 
      acaagggac ggaggcagt ctccggtta atctagagc 
      cgaataagc tgctcttcg acccaaacc ttcgacccc 
      ttcacatcg ataaacaca gggtgagcg gttccatca 
      tgaatacgg gccatagtg taatattta cagtagata 
      acgacactc ggcatcaca taaatgtga ctgggggtc 
      cactatcct ataaatatc agtttcaag aaatccata 
      ttcactacg gactccacg atcagtttg ggtccgaac 
      attcaataa atggataca acttccctg cagagatac 
      agattttcg ggttcaact cgcaactct acaccaact 
      tctcggaag cgctccaca aagctcaag gcatcgtgg 
      ttatttctc ttctcttac agatgggtg acctttcca 
      atcccgagc tgaggatat taacagatc agctagaaa 
      aaattagat acggcgggg acgaggtcg aggtgaaac 
      gcctttcag tgcgaggtc tacttcctg aaaccgact 
      attacatga cgtacgatg gatccacga cgatgccgg 
      cctgccacg agaccgtca catggttga tcctcaaca 
      agcctattt acatcacca agcaacaga tgcatgcgt 
      tacgcagac tgatgcaag gagatgcca aacacccca 
      aagccccgt tgggaaaca acttccgac caccacagc 
      ccctgcacc atcgaccag ttcggacta atattgact 
      tcagcgtta aacaccgca ctgacgcgg gaaagcaat 
      gtcccagca tgtacaaag acgtctact ataaggcaa 
      acagttgga aacagcact gagtgtcgc catatctag 
      tgaacaatc catatcact ccaatatca tcaagactc 
      tagtgacgt gaccagaac aattctagt gctaaatta 
      aacgtcgac aaaataaaa catatcata ccttgcaaa 
      gagatgtaa taaatgact gaattatgt gttaagcta 
      acgtgataa atgcttaaa aatagctta gctgataga 
      aacttaata gactgtttt cgttttcgg tttttgttt 
      gtcgataaa gttattgtc ggtttcgtt ggcctaaat 
      gcacattat ttaaaaggg aaataaaaa tggaagtgt 
      tat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY