Status
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Gene information
General information
TC032876
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007184
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7885693..7887023
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032876
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04291

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032876 RA
      MLLLKAIPS VFLLVYCSA APAAPSDDD DAHLTVPEL 
      ITKYGYPVE VHQVTTTDG YILTLHRIP HGKNTDKVS 
      NRVVFLMHG LLCSSADWI FTGPDHGLG YLLADEGYD 
      VWMGNARGN HQSRNHTRL NPDKDPEFW QFSWHEIGA 
      VDVPAMIDH VLEVTGEES LYHIGHSQG TTTFYVMTS 
      MRPEYNSKI KAHFSLAPV GYTNHMTSP LMHILAFWS 
      GPLELLFKL IGVNEFLPT NEFLALMGD TFCRDGDIT 
      QFLCSNALF AICGFSPKE MNATLFPVL TAHTPAGSS 
      TRQLVHYAQ GINTGTFRQ FDFGLKNLE IYGTFTPPA 
      YDLKLITAP IYLFYSHND WMAAERDVI RLCNGLGDS 
      CKGKFLVSD NSFNHLDYL YGISAPKIV YERVISLMA 
      RH
    CDS sequence
    • >TC032876 RA
      atgcttcta ttaaaagca attccaagt gtcttcctc 
      cttgtgtat tgctcagct gccccagcc gctccatcc 
      gatgatgac gatgcccac ctgactgtt ccggaactc 
      atcaccaaa tacggatat cccgtagag gtgcaccag 
      gtcaccaca actgatggc tacatcctg accctccat 
      cgcatcccc cacggcaag aacactgat aaagtatca 
      aacagagtc gttttcctg atgcatgga ctcttgtgc 
      tcttctgcc gactggatc ttcaccggg cctgaccac 
      ggcctcggc tacctcctg gcagacgaa ggctacgac 
      gtttggatg ggcaatgct cgaggaaac caccaatcg 
      cgcaaccac actcgcctt aaccccgac aaagacccc 
      gaattttgg cagttcagt tggcacgaa atcggggct 
      gtggacgtc cctgccatg atcgaccac gtgctggag 
      gtcacaggc gaggaaagc ctctaccac atcgggcac 
      tcgcaagga accacaact ttctacgtc atgacctcc 
      atgcggccg gaatacaac agcaagatc aaggcacat 
      ttcagtcta gcccccgtg gggtacacg aaccacatg 
      accagtccc ctaatgcat attttggcg ttctggtcc 
      gggcctctc gagctcctc ttcaaactg atcggagtg 
      aacgaattt ctcccaact aacgagttt ttggcattg 
      atgggcgat acgttttgt cgcgacggg gacataacc 
      cagtttctg tgctcaaat gcgcttttt gccatttgt 
      gggttcagt ccgaaggag atgaatgcg acccttttc 
      cctgttttg acagcacat actcctgct gggtcttcg 
      accagacaa ttggtccat tacgcgcag ggaattaac 
      acggggacg ttccggcag tttgatttt gggttgaaa 
      aatctggag atttacggg actttcact ccacctgcc 
      tacgacttg aagcttatt acagcaccg atttatctc 
      ttctacagt cacaatgac tggatggcg gctgaacgg 
      gacgttatc aggctttgt aacgggtta ggggacagt 
      tgtaaaggc aaattttta gtgtctgac aattctttc 
      aatcacttg gattacttg tacgggatc agtgcccct 
      aaaattgta tacgaacgg gtgattagt ttaatggca 
      aggcactga
    mRNA sequence
    • >TC032876 RA
      atctaactc cgagttgcc aaaatgctt ctattaaaa 
      gcaattcca agtgtcttc ctccttgtg tattgctca 
      gctgcccca gccgctcca tccgatgat gacgatgcc 
      cacctgact gttccggaa ctcatcacc aaatacgga 
      tatcccgta gaggtgcac caggtcacc acaactgat 
      ggctacatc ctgaccctc catcgcatc ccccacggc 
      aagaacact gataaagta tcaaacaga gtcgttttc 
      ctgatgcat ggactcttg tgctcttct gccgactgg 
      atcttcacc gggcctgac cacggcctc ggctacctc 
      ctggcagac gaaggctac gacgtttgg atgggcaat 
      gctcgagga aaccaccaa tcgcgcaac cacactcgc 
      cttaacccc gacaaagac cccgaattt tggcagttc 
      agttggcac gaaatcggg gctgtggac gtccctgcc 
      atgatcgac cacgtgctg gaggtcaca ggcgaggaa 
      agcctctac cacatcggg cactcgcaa ggaaccaca 
      actttctac gtcatgacc tccatgcgg ccggaatac 
      aacagcaag atcaaggca catttcagt ctagccccc 
      gtggggtac acgaaccac atgaccagt cccctaatg 
      catattttg gcgttctgg tccgggcct ctcgagctc 
      ctcttcaaa ctgatcgga gtgaacgaa tttctccca 
      actaacgag tttttggca ttgatgggc gatacgttt 
      tgtcgcgac ggggacata acccagttt ctgtgctca 
      aatgcgctt tttgccatt tgtgggttc agtccgaag 
      gagatgaat gcgaccctt ttccctgtt ttgacagca 
      catactcct gctgggtct tcgaccaga caattggtc 
      cattacgcg cagggaatt aacacgggg acgttccgg 
      cagtttgat tttgggttg aaaaatctg gagatttac 
      gggactttc actccacct gcctacgac ttgaagctt 
      attacagca ccgatttat ctcttctac agtcacaat 
      gactggatg gcggctgaa cgggacgtt atcaggctt 
      tgtaacggg ttaggggac agttgtaaa ggcaaattt 
      ttagtgtct gacaattct ttcaatcac ttggattac 
      ttgtacggg atcagtgcc cctaaaatt gtatacgaa 
      cgggtgatt agtttaatg gcaaggcac tgattttat 
      tctcacgac aaaaatata ctgtgtata tgtttaagt 
      taataaaaa cgttagaaa ccc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY