Status
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Gene information
General information
TC007172 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8112105..8118996
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007172
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04289

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007172 RA
      MAAEPNGSQ EQNTTSIQP TFSPTLDPV ARRELEKEF 
      YETYDVMTG VRIAATLGG FFGLMVILV LYKSKSKTE 
      KALEDPDFT AAAVAEVEE EERQLAAVL EATAYQQLN 
      SRKGRRSLD TSSVPPGWI KTTARFSSV GGYSSLMEP 
      PTRTHSRLP CFIDEDSPP EEDQSLYDD VCYNGKLDV 
      PRRPSNITC SSSGSSYLE RRDSAVAIG LPVLPGHKS 
      KNRRRQSSP LLEIYDFYY PIDIKVIQP TPGGSPCGS 
      DRALYDHVT DPPNLIPRL APLASISSC NSSFGADYT 
      DLDVASYTS DSVFQEERD NTDDEVDEF STDSDLTDA 
      GACYNRRKK KNSICDSLV PTPVNEGPS CVSPKISIK 
      SSSKSSVTV IETVDQPEM PATESIRKS STSSSLNCE 
      EPGAQETLF
    CDS sequence
    • >TC007172 RA
      atggcggcg gagccaaat ggctcgcag gaacagaat 
      accacttca attcagccc actttttct ccaacactt 
      gatcctgtg gcacgaaga gaactggaa aaggagttt 
      tacgaaacg tacgatgtt atgaccgga gttcggatc 
      gctgcaacc ttgggcggt ttctttggt ttgatggtg 
      attcttgtt ttatacaaa tcgaaatca aagactgaa 
      aaagccttg gaagatcca gattttacg gcagcggcg 
      gttgccgaa gtcgaggag gaggaaaga cagctcgct 
      gcggtgttg gaagccact gcttatcaa caattaaat 
      tcaaggaaa ggacgaagg tcgctggac acaagcagc 
      gtacctcca ggctggatt aaaaccaca gcgaggttc 
      tcctctgtt gggggatat agcagctta atggagccc 
      cccacaaga actcattcc cgactgcca tgctttatt 
      gatgaagat tctcctcca gaggaggac cagtcgctt 
      tatgacgac gtttgctac aatggaaaa ctggacgtc 
      ccccgaagg cccagcaac ataacgtgc tcgtcttca 
      ggcagctct tacttggag cgccgtgat tccgcggtg 
      gcgataggg cttccggtg ttgccggga cacaagtcg 
      aagaatcgt cgccgtcag tcatcgcct cttctcgaa 
      atttacgac ttttattat cccattgac atcaaagta 
      atccaacca acgcccggg ggatccccg tgtggcagt 
      gaccgggct ctgtacgat catgtcaca gatccgcca 
      aacttgata ccacgtctc gcaccactg gcgtctata 
      agtagttgt aatagttcg tttggtgct gattacaca 
      gacttggat gtcgcgtct tacacaagt gactccgtt 
      tttcaagaa gaaagggat aatactgac gatgaagtt 
      gatgagttt tcgactgac agcgactta accgacgcg 
      ggcgcttgt tataatcgc aggaaaaag aaaaatagt 
      atttgtgat tcgttggta cccacgcct gttaacgaa 
      ggaccttct tgcgtttcg ccaaaaatt agcattaag 
      tcttcgtct aagtcttct gtgacggtg atcgaaact 
      gttgatcag cccgaaatg ccagcgact gaaagtatt 
      aggaaatca tcgacttcg tcttctctc aactgtgag 
      gaaccagga gcacaagag actcttttt tag
    mRNA sequence
    • >TC007172 RA
      attttcagt agtggctgt catgtcgaa ataaatgaa 
      catgctttt aaaagcttt tataaaaac caataaatg 
      gacaataaa tccaagtgt accatccga attaatcat 
      gtgaactca tcaaccgaa acgaaaaca tgtcgcaca 
      atgtaaatc gttgaaaat ttacaaaat ttcgtattt 
      ttttaaaaa tgtaaaact aggctgttt taaataatg 
      tgcttaatt atcgatgat gatgagcaa ataatttta 
      gtggtttac gtatcgccc gtgttttcc ttcaccaat 
      cctaaaaat agctctagc cagcttgag agaataaat 
      gtgttcaag aacggggag tttttcagc attttctga 
      aacatattc ggttgccgg tttaagagt cggactcaa 
      agtgggtga cttttcaaa cttagtgat gggttttgt 
      gtagtgtta tggacgtag cgcaaaatt tcaattcgg 
      ttgtttttc atgttttta ttatgagag gggcccaac 
      gtacgtgat ggcggcgga gccaaatgg ctcgcagga 
      acagaatac cacttcaat tcagcccac tttttctcc 
      aacacttga tcctgtggc acgaagaga actggaaaa 
      ggagtttta cgaaacgta cgatgttat gaccggagt 
      tcggatcgc tgcaacctt gggcggttt ctttggttt 
      gatggtgat tcttgtttt atacaaatc gaaatcaaa 
      gactgaaaa agccttgga agatccaga ttttacggc 
      agcggcggt tgccgaagt cgaggagga ggaaagaca 
      gctcgctgc ggtgttgga agccactgc ttatcaaca 
      attaaattc aaggaaagg acgaaggtc gctggacac 
      aagcagcgt acctccagg ctggattaa aaccacagc 
      gaggttctc ctctgttgg gggatatag cagcttaat 
      ggagccccc cacaagaac tcattcccg actgccatg 
      ctttattga tgaagattc tcctccaga ggaggacca 
      gtcgcttta tgacgacgt ttgctacaa tggaaaact 
      ggacgtccc ccgaaggcc cagcaacat aacgtgctc 
      gtcttcagg cagctctta cttggagcg ccgtgattc 
      cgcggtggc gatagggct tccggtgtt gccgggaca 
      caagtcgaa gaatcgtcg ccgtcagtc atcgcctct 
      tctcgaaat ttacgactt ttattatcc cattgacat 
      caaagtaat ccaaccaac gcccggggg atccccgtg 
      tggcagtga ccgggctct gtacgatca tgtcacaga 
      tccgccaaa cttgatacc acgtctcgc accactggc 
      gtctataag tagttgtaa tagttcgtt tggtgctga 
      ttacacaga cttggatgt cgcgtctta cacaagtga 
      ctccgtttt tcaagaaga aagggataa tactgacga 
      tgaagttga tgagttttc gactgacag cgacttaac 
      cgacgcggg cgcttgtta taatcgcag gaaaaagaa 
      aaatagtat ttgtgattc gttggtacc cacgcctgt 
      taacgaagg accttcttg cgtttcgcc aaaaattag 
      cattaagtc ttcgtctaa gtcttctgt gacggtgat 
      cgaaactgt tgatcagcc cgaaatgcc agcgactga 
      aagtattag gaaatcatc gacttcgtc ttctctcaa 
      ctgtgagga accaggagc acaagagac tctttttta 
      gattttggt tttttgtat atgactaaa aacagtcaa 
      ggactaaat accaataat aagtgctag ggtactagg 
      ttacactca atgttgtca tttaagaaa ttgtgtaac 
      agaactttc tttccagtc ctaccaaac tagactgaa 
      ctgtctcca gcaagattt tttagaaga aaaatattg 
      gcaattttt tcgtgacta tttttacag agtatgaaa 
      ctagaatta tgcgtttta tgttataca ttagaaacg 
      taaataaat agtatcaac aaactgtta gaaatgtct 
      tcacaacgt gtatttacc atattttta cgttttact 
      acaatcctt taatttgta aagataaat gtgattaaa 
      taaaaagtt gtggtttga aaagttctt cacaaattg 
      cctccaaac ctttaacca tttaaccac catgatata 
      tttaccaga acgcgagca atttcagaa tgtacaata 
      agttgccaa aacaatgca aacgttttc tctattcaa 
      aataattgt ggcttgtga tgaaacagg gtgagtgtt 
      aaatgtatg tttttatcc gcacagtcg ttttttagt 
      aaaaaacaa cgaatttat ttctacccc gttgcaaca 
      attatttaa ttttttccc acctgttcc gaatttata 
      ctcagatcg catttaata ttaaacata tgtgaaaca 
      tatcgttaa gtcgtagat tgtttgctg tgtttgggg 
      aagaacact gaggtgtat tccaattgc ggttgtttg 
      atgtcaggg tcttgttgt gtcaaacat agtttttta 
      catagtgta tcttgaagt gttacgttg ttaaaaatt 
      cgtacaaat atttgggcg caaacattt gaaaatgta 
      tagtttagt gtgtgaata gcattgatt gtacgattt 
      cctcttcgt tgaaaccat tgttaaaaa agatgggaa 
      atcatacaa ccctctttg tttctgttt agaatatta 
      aagaaattt tatattttt acatataaa atataccta 
      taggccttt tgtgtcagt aaaatgctt ttcaggaga 
      ttattgtat gttgttctg tttgtttat aaatggtgg 
      taaaaaata aatgtaatg tttattg
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