Status
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Gene information
General information
TC007168 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8140344..8142082
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007168
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04288

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007168 RA
      MCRACLSGE NEIMSAFDS ELRDMFIFC TSLELSSDD 
      KLPQSICNK CKEQLVNAH TFKNTCIKS NTLLKQHLL 
      SNYEIDDLD LKLDDLFKI DFDENCDES KPNSPQPLK 
      PPELNNQKP RRQNKNFKF HCETCSEGF NLEDDLVLH 
      RIAHPKDGI PKCTICDKL FNTLKVLKR HVRIHFKSK 
      PFECDLCSH TFTESGSLT RHLRKHRGE KRHLCTVCG 
      KGFYEANVL AVHMRTHTG EKPIKCEIC GKRFADPNG 
      LRSHIKSHS GEKKYSCKI CNKCFSHSF VLKKHLRIH 
      SGERPYLCP VCGKSFTQS HYVSVHMRQ HTGELPYSC 
      TVCKKRFVQ NSQLEMHLR THTGYKPYI CNVCGKACA 
      RSGDLVIHM RTHTGEKPF TCTVCLKSY TTSSHLKVH 
      LRTHNGERS HLCTFCGKS FTENQGLKA HIRTHTGEK 
      PYICAVCGH GFAQSGALA THRKIHKVG TGLKADDEI 
      KIENNPLY
    CDS sequence
    • >TC007168 RA
      atgtgtaga gcatgctta tcaggcgaa aatgagata 
      atgtcagcc tttgattcg gaattgaga gacatgttc 
      atattttgc acgtcctta gagttgtcc agtgacgac 
      aagttgccc caatcaatc tgtaataaa tgcaaagag 
      caacttgta aacgcccac acattcaaa aacacctgt 
      atcaagtca aacaccctt ctaaagcaa catttatta 
      agcaattac gaaatcgat gacttagat ttaaaactt 
      gatgatctc ttcaaaatc gactttgac gaaaattgt 
      gatgagtcc aaacctaac tcaccccaa cccttaaaa 
      ccccccgaa ctcaacaac caaaagcca cgtcgtcag 
      aataaaaac ttcaagttt cactgcgag acatgttcg 
      gagggtttt aacttagag gatgactta gttttgcat 
      cgcatagct catccaaaa gacggcatt cccaaatgc 
      acaatttgt gataaatta ttcaacaca ctaaaggtc 
      ctaaaacgc cacgttcgc atacatttc aagtcgaaa 
      ccgtttgag tgtgatctc tgttcgcac actttcaca 
      gaatcggga agtttgacc cggcatttg aggaaacac 
      aggggcgaa aagcggcat ttgtgcacc gtgtgcggt 
      aaaggcttt tacgaagcc aacgtctta gcagttcat 
      atgaggaca cacacgggg gaaaaaccg atcaaatgt 
      gaaatttgc ggcaaaaga ttcgcagat ccgaatgga 
      ctgaggagc cacatcaag tcgcattca ggcgagaag 
      aaatattcg tgcaaaata tgtaataag tgtttttcg 
      cattcgttc gttttgaaa aaacatttg aggatacat 
      agcggggaa agaccctac ttgtgtcca gtctgtggt 
      aaatcattc acccagagc cattatgtc tccgtacat 
      atgaggcaa cataccggg gaattaccg tacagttgc 
      acagtttgt aaaaaaaga tttgtacaa aacagccaa 
      ctagaaatg catctgcgg acacacaca ggctacaaa 
      ccgtacatt tgcaatgtg tgcgggaaa gcttgtgcc 
      cgctctggt gatttggta attcacatg cggacacac 
      acgggtgaa aaacctttc acttgcacg gtttgtctg 
      aagagttac acaacttcc agtcattta aaagtgcac 
      ctgaggaca cataatggg gagaggagt catctttgc 
      actttttgc ggcaagagt tttacggag aatcagggg 
      ttgaaggcc catattaga acacatact ggcgaaaaa 
      ccgtatatt tgtgcggtt tgtgggcat gggtttgca 
      cagtcgggg gctttggct actcatagg aagatacac 
      aaagtgggg acggggctt aaggctgat gatgaaatc 
      aaaattgag aataacccg ctttattaa
    mRNA sequence
    • >TC007168 RA
      cagatgtca ggtattccg gtgacaggt gtgttcaat 
      aataatgaa taatttcga tgtaaataa cgttgataa 
      gatgtgtag agcatgctt atcaggcga aaatgagat 
      aatgtcagc ctttgattc ggaattgag agacatgtt 
      catattttg cacgtcctt agagttgtc cagtgacga 
      caagttgcc ccaatcaat ctgtaataa atgcaaaga 
      gcaacttgt aaacgccca cacattcaa aaacacctg 
      tatcaagtc aaacaccct tctaaagca acatttatt 
      aagcaatta cgaaatcga tgacttaga tttaaaact 
      tgatgatct cttcaaaat cgactttga cgaaaattg 
      tgatgagtc caaacctaa ctcacccca acccttaaa 
      accccccga actcaacaa ccaaaagcc acgtcgtca 
      gaataaaaa cttcaagtt tcactgcga gacatgttc 
      ggagggttt taacttaga ggatgactt agttttgca 
      tcgcatagc tcatccaaa agacggcat tcccaaatg 
      cacaatttg tgataaatt attcaacac actaaaggt 
      cctaaaacg ccacgttcg catacattt caagtcgaa 
      accgtttga gtgtgatct ctgttcgca cactttcac 
      agaatcggg aagtttgac ccggcattt gaggaaaca 
      caggggcga aaagcggca tttgtgcac cgtgtgcgg 
      taaaggctt ttacgaagc caacgtctt agcagttca 
      tatgaggac acacacggg ggaaaaacc gatcaaatg 
      tgaaatttg cggcaaaag attcgcaga tccgaatgg 
      actgaggag ccacatcaa gtcgcattc aggcgagaa 
      gaaatattc gtgcaaaat atgtaataa gtgtttttc 
      gcattcgtt cgttttgaa aaaacattt gaggataca 
      tagcgggga aagacccta cttgtgtcc agtctgtgg 
      taaatcatt cacccagag ccattatgt ctccgtaca 
      tatgaggca acataccgg ggaattacc gtacagttg 
      cacagtttg taaaaaaag atttgtaca aaacagcca 
      actagaaat gcatctgcg gacacacac aggctacaa 
      accgtacat ttgcaatgt gtgcgggaa agcttgtgc 
      ccgctctgg tgatttggt aattcacat gcggacaca 
      cacgggtga aaaaccttt cacttgcac ggtttgtct 
      gaagagtta cacaacttc cagtcattt aaaagtgca 
      cctgaggac acataatgg ggagaggag tcatctttg 
      cactttttg cggcaagag ttttacgga gaatcaggg 
      gttgaaggc ccatattag aacacatac tggcgaaaa 
      accgtatat ttgtgcggt ttgtgggca tgggtttgc 
      acagtcggg ggctttggc tactcatag gaagataca 
      caaagtggg gacggggct taaggctga tgatgaaat 
      caaaattga gaataaccc gctttatta aatttctct 
      attgactga atgttgttt tttattttc ccttctccc 
      gaaatgaac acattggtg atttaatat agagcacaa 
      aaacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY