Status
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Gene information
General information
TC007167 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8147113..8149482
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007167
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04287

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007167 RA
      MKLLRAAKR VISSSSQQQ LGVISAIEE NHCKEWADA 
      VPYEEVPGP KPLPFLGNT WRFIPFIGD FQIEHIDKV 
      SKKLYEKYG KIVKMQGLL GRPDMLFLF DPDEIEKVF 
      RQEDTLPYR PSMPSLNYY KHVHRKEFF GDNCGVIAV 
      HGDRWQNFR TKVNQIMLQ PRVTRMYVK SIETTSQEL 
      VDRIEVIKN KKSEVPDDF LNELHKWSL ESIAKIALD 
      VRLGCLDPN AHPDTQTLI NAINTFFMN VPILELKIP 
      FWKIWNTPT FKEYLHALD TIREITLKH VDIALHKLS 
      QDDCEELSV LQRVLKMEN DAKTASNLA LDMFLVGVD 
      TTSNAVASI LYQLSLHQE KQAKLYEEI ASLLPCKST 
      QISHEKLDQ MHYLKACIK ETMRMYPVV IGNGRCTTR 
      DRVIGGYQI PKGVQVIFQ HCVISNLEE YFPRSGEFL 
      PERWLKNSE LFRNHHPFA SLPFGFGKR MCLGRRFAD 
      LEIQTVIAK VVRNFKIEY HHDKLDYHI HPMYTPYGP 
      LRLTFIRRQ
    CDS sequence
    • >TC007167 RA
      atgaaattg ctaagagcc gcaaaacgt gtaattagt 
      agttcgagt cagcaacaa ctaggtgtg ataagtgca 
      attgaggag aatcattgt aaggaatgg gcagatgcc 
      gtgccctat gaagaggtc cccggaccc aaacctttg 
      ccttttttg ggcaatacg tggagattt attcctttt 
      ataggcgat ttccaaatc gaacacatc gacaaagta 
      tcaaaaaaa ttatacgaa aaatatgga aaaatcgtc 
      aaaatgcag ggcttgcta gggcgccct gacatgctc 
      ttcctcttc gatcccgac gaaatcgag aaggttttt 
      cgccaggaa gacactctc ccctatcgc ccttccatg 
      ccctccctt aattattac aaacacgtc caccgaaaa 
      gaatttttc ggcgacaat tgtggcgta atcgccgta 
      catggggac aggtggcag aactttagg acgaaagtg 
      aaccaaata atgttacaa ccgcgagtt actcgaatg 
      tatgtcaag tccattgag acgacatcc caagaattg 
      gtcgacagg attgaagtg atcaagaat aagaaaagc 
      gaagtgcct gatgatttc ctcaacgag cttcacaag 
      tggtctttg gagtcaatc gccaaaatc gcacttgat 
      gtccgactg ggttgcctg gaccccaac gcccacccc 
      gacacccaa acattgatt aacgccatc aacaccttc 
      ttcatgaat gtcccgatc ctggagctg aaaatccca 
      ttttggaaa atctggaat actccgact ttcaaggaa 
      tatttgcac gccttggat acaattcga gagataaca 
      ctaaaacat gttgacatt gcgttgcat aaattatcc 
      caagacgat tgtgaagag ctttcagtg ttacaacga 
      gttctaaaa atggaaaac gatgccaaa acagcatct 
      aatttagcg ctagacatg ttcctcgtc ggggttgac 
      accacctca aacgcggta gcgtcgatc ctctaccag 
      ctctccctg caccaggaa aagcaggca aaactttat 
      gaagaaatc gcaagtctt ctaccgtgc aaatccacg 
      caaatatct cacgagaaa ctagaccaa atgcattac 
      ctgaaagcg tgcattaaa gaaaccatg agaatgtat 
      ccggtggtg attggtaat ggcagatgc acaacgaga 
      gatcgggta attggaggc tatcagata ccaaaaggc 
      gtgcaggtg atatttcaa cactgcgtc attagcaac 
      ctagaggag tactttcca cgaagtggt gagtttctc 
      ccagagagg tggctcaaa aattcggaa ctgttccgc 
      aaccatcat cctttcgca tcgctaccg ttcggcttc 
      ggcaaaagg atgtgtctg ggacggagg tttgcggac 
      ttggaaatc caaacagtc atagccaag gttgtgcgt 
      aatttcaaa atcgaatac catcacgat aagcttgat 
      taccatatt catcccatg tacactccc tatggacct 
      ttacgactg acgtttatt cgccgacag taa
    mRNA sequence
    • >TC007167 RA
      tccaaatat gatagtaca gtagttaca agctttttt 
      ttttcgaaa aatgaaatt gctaagagc cgcaaaacg 
      tgtaattag tagttcgag tcagcaaca actaggtgt 
      gataagtgc aattgagga gaatcattg taaggaatg 
      ggcagatgc cgtgcccta tgaagaggt ccccggacc 
      caaaccttt gcctttttt gggcaatac gtggagatt 
      tattccttt tataggcga tttccaaat cgaacacat 
      cgacaaagt atcaaaaaa attatacga aaaatatgg 
      aaaaatcgt caaaatgca gggcttgct agggcgccc 
      tgacatgct cttcctctt cgatcccga cgaaatcga 
      gaaggtttt tcgccagga agacactct cccctatcg 
      cccttccat gccctccct taattatta caaacacgt 
      ccaccgaaa agaattttt cggcgacaa ttgtggcgt 
      aatcgccgt acatgggga caggtggca gaactttag 
      gacgaaagt gaaccaaat aatgttaca accgcgagt 
      tactcgaat gtatgtcaa gtccattga gacgacatc 
      ccaagaatt ggtcgacag gattgaagt gatcaagaa 
      taagaaaag cgaagtgcc tgatgattt cctcaacga 
      gcttcacaa gtggtcttt ggagtcaat cgccaaaat 
      cgcacttga tgtccgact gggttgcct ggaccccaa 
      cgcccaccc cgacaccca aacattgat taacgccat 
      caacacctt cttcatgaa tgtcccgat cctggagct 
      gaaaatccc attttggaa aatctggaa tactccgac 
      tttcaagga atatttgca cgccttgga tacaattcg 
      agagataac actaaaaca tgttgacat tgcgttgca 
      taaattatc ccaagacga ttgtgaaga gctttcagt 
      gttacaacg agttctaaa aatggaaaa cgatgccaa 
      aacagcatc taatttagc gctagacat gttcctcgt 
      cggggttga caccacctc aaacgcggt agcgtcgat 
      cctctacca gctctccct gcaccagga aaagcaggc 
      aaaacttta tgaagaaat cgcaagtct tctaccgtg 
      caaatccac gcaaatatc tcacgagaa actagacca 
      aatgcatta cctgaaagc gtgcattaa agaaaccat 
      gagaatgta tccggtggt gattggtaa tggcagatg 
      cacaacgag agatcgggt aattggagg ctatcagat 
      accaaaagg cgtgcaggt gatatttca acactgcgt 
      cattagcaa cctagagga gtactttcc acgaagtgg 
      tgagtttct cccagagag gtggctcaa aaattcgga 
      actgttccg caaccatca tcctttcgc atcgctacc 
      gttcggctt cggcaaaag gatgtgtct gggacggag 
      gtttgcgga cttggaaat ccaaacagt catagccaa 
      ggttgtgcg taatttcaa aatcgaata ccatcacga 
      taagcttga ttaccatat tcatcccat gtacactcc 
      ctatggacc tttacgact gacgtttat tcgccgaca 
      gtaaagttc aagacgatc attattgtt actttttta 
      caataaaat ttattctca cag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY