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Gene information
General information
TC007150 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8435953..8439330
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007150
    iBeetle screen iB_04286

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007150 RA
      MEFLLNLLL LRGKVLTTA AAETLHEYI KKCMHFKRK 
      ISTEYSNAT QIIPFENML EFINNSSKY TCEGDVVTN 
      DLRYLYEHI NRVKQENEK HTHTVALVP QTVDKKLEE 
      LPKNQNLNT VPEWLRREH TKINEFKVK SQEHIIDNL 
      HAILKSQEF LFKNIIKLV NILEKDALI NSNSEKLDK 
      IKRKLVACT DILSNAATS QQFTSLINK IIEEEITRC 
      RFRHGESKT SAEINLKVP KPEGIEAAL DTSHKNNDI 
      RRMKKQLAE KIAKPNVQN KQNERLRGR IIDLEVKTN 
      SAKLLKRAR SEPVLNENR KLETIWKKT QIEIEESKK 
      VIQVNEDRF KKFENTLWH TLTTMKFLS LKSDYANMT 
      KCAFYNKRQ FVATEATIS KKVQGEEKF GKFVKISNS 
      MFHITEKRL SFEEGQLDG VGESGGANN KKTEVSKDK 
      IFPKKSVSN APPVLIQNQ KKDEQGNNT IINGNDIKK 
      LVQNHSANA TVAVVVAGG DKSVIKSRE IVPTERDNK 
      QTNTIISVH NFIKKTGEV SKNVAPLDA IKKDDQKTI 
      LEKEGTSKA LNPIRTEDE RKTTPQKNP QKTEEQQIV 
      KSKDNEPKF AVPEKIERM NQQTTDQRF VQSLRNSDL 
      NKKPCKTTR NVKMRLNVI LKTKDSLGT KRTTAPPPL 
      IKSSKKESL FNNVGANTK LQTIASDNL KLCTLNAVS 
      PKKAKDESK KVSMHEFKS EADLIHMNN KAFQELETT 
      IKTVQIVKP NGKTQNVKK QEKKSVSSE NLASIMKQR 
      NDIKREMKS EASSRAGGD EKISRKNKR DLHTEVKQL 
      SKEKCKTFL KTSRIKSFV KPNPSNQQM RRTTKNAMK 
      RQQVLTILP KVAKEIKEK KKPQVCLVE PGEIKTVKM 
      KDSYCVSKK SSYVKFSKK NLPNKKINQ TSWEEEDRY 
      KVASVVED
    CDS sequence
    • >TC007150 RA
      atggaattc ctgttaaat ttattgtta ctgcgtggg 
      aaggtttta accacagca gcggccgag acactccac 
      gaatatatc aaaaaatgt atgcatttc aagcgaaaa 
      atttcgacc gaatacagc aacgcgacc caaataatc 
      ccgtttgaa aacatgttg gagtttatc aacaactcg 
      agtaaatac acctgtgag ggagacgtt gtgacgaac 
      gatctccgc tacttgtac gaacacata aaccgggta 
      aagcaagaa aatgagaaa catacacat actgttgct 
      ttggtgccg caaaccgtt gataaaaaa ttggaagag 
      ttgccgaaa aaccaaaat ttgaacaca gtccctgaa 
      tggttgcgc cgtgaacac accaaaatt aatgagttt 
      aaagtgaaa agtcaggaa cacatcatt gacaacttg 
      cacgcaatc ctcaaatcg caagagttt ctcttcaag 
      aatatcatc aaactggtg aacatcctc gagaaggat 
      gccctcatc aattcaaac agcgaaaaa ctggacaaa 
      atcaagagg aaacttgtc gcatgcact gatattttg 
      tcaaacgca gccacttcg caacaattc acaagcctc 
      atcaataaa ataatcgaa gaagagatc acgcgttgt 
      agattcagg cacggcgaa agcaaaacc agcgctgaa 
      attaattta aaagtgccg aaacctgag ggaatcgaa 
      gctgctttg gacacgtcc cacaagaac aacgacatc 
      agaagaatg aagaaacaa ctcgctgag aaaattgcg 
      aaaccaaac gtacaaaat aaacagaat gagcgttta 
      aggggccgt attatcgac ctggaagtg aaaacaaat 
      tcggccaag ttactaaaa cgggcgaga tccgagcca 
      gttttgaac gaaaaccga aaattggaa accatctgg 
      aagaaaaca cagattgag attgaagaa tccaaaaaa 
      gtgatacaa gtcaatgaa gaccgtttc aagaaattt 
      gaaaacaca ctttggcac accttaaca accatgaaa 
      tttttgtcg ctgaaaagt gattacgca aatatgacc 
      aagtgtgcg ttctataat aagagacag tttgtggcg 
      acggaagca acaatttcg aaaaaagtc caaggagag 
      gaaaagttc ggcaagttt gttaagata agtaactcc 
      atgttccac attacggaa aaacgtctg tcgtttgag 
      gagggacaa ttagatggt gttggagaa agtggagga 
      gcaaataat aaaaagaca gaagtgtct aaagacaag 
      atttttcca aaaaaatct gttagtaat gctccaccc 
      gtgcttata caaaaccag aaaaaagac gaacagggc 
      aataatacc attataaat ggcaatgac atcaaaaaa 
      ttggttcag aaccattcc gcaaatgcc actgttgcg 
      gtagttgtt gcaggaggt gacaaaagt gtgatcaag 
      tctcgagaa atagtacca acggaacga gacaacaaa 
      caaactaac acaattatt agtgttcat aattttatt 
      aagaaaaca ggcgaggtt tctaaaaat gttgcacct 
      ttagatgca attaagaaa gatgatcag aaaactatt 
      ctggagaaa gaaggaaca agtaaagct ttaaatccc 
      attagaaca gaagatgag aggaaaact actccacag 
      aaaaatcca caaaaaact gaggagcaa caaattgtt 
      aaaagtaaa gataatgaa cccaaattt gctgtaccc 
      gaaaaaata gaaagaatg aatcaacaa acaacagat 
      cagcgtttc gtacagtcg ttaagaaat tcggacttg 
      aataagaag ccttgcaaa acaactaga aatgttaaa 
      atgaggttg aatgtcatc ctaaaaacg aaggattct 
      ttgggaaca aagcgcact acagctcca cctccgtta 
      attaagagt tctaaaaaa gaatcctta tttaacaat 
      gttggagca aacacaaaa ctccaaact attgccagc 
      gataattta aaattgtgc actttgaac gcagtttct 
      cctaaaaaa gctaaagac gaaagcaaa aaagtgtcc 
      atgcacgaa tttaaaagc gaggctgat ctcatccat 
      atgaacaat aaagcattt caggaacta gaaactaca 
      attaaaacg gttcagatt gttaaacca aatggaaaa 
      acacaaaat gttaagaag caggagaaa aaaagtgtg 
      tctagtgaa aatttggca tcgattatg aaacagcgg 
      aatgatatt aaacgcgaa atgaaatcc gaggcgtct 
      tcgcgagct ggaggtgat gagaaaata agccgcaaa 
      aataagcgt gatttgcat acagaagtc aagcaactg 
      tctaaagaa aaatgtaaa acgtttttg aaaacttcg 
      aggataaaa tcgttcgtg aaaccaaac cccagtaat 
      caacaaatg cgccgtacg acgaaaaat gcgatgaag 
      agacagcaa gttttgaca attttacct aaagttgcg 
      aaagaaatt aaagagaaa aaaaagccg caagtttgt 
      ttggttgaa cccggggaa ataaaaacc gtgaaaatg 
      aaagacagt tattgcgtg tccaaaaag tcaagttac 
      gtgaaattt tctaaaaaa aatttgccc aacaaaaaa 
      attaatcag acgagttgg gaagaagag gataggtac 
      aaggttgcc tcagtggtg gaagactaa
    mRNA sequence
    • >TC007150 RA
      tggcgcgtt gtcaaagaa ttcaaaaaa tgtggccga 
      gcaaataat tccaatcat ttcaatttc atggcgcaa 
      attatggaa ttcctgtta aatttattg ttactgcgt 
      gggaaggtt ttaaccaca gcagcggcc gagacactc 
      cacgaatat atcaaaaaa tgtatgcat ttcaagcga 
      aaaatttcg accgaatac agcaacgcg acccaaata 
      atcccgttt gaaaacatg ttggagttt atcaacaac 
      tcgagtaaa tacacctgt gagggagac gttgtgacg 
      aacgatctc cgctacttg tacgaacac ataaaccgg 
      gtaaagcaa gaaaatgag aaacataca catactgtt 
      gctttggtg ccgcaaacc gttgataaa aaattggaa 
      gagttgccg aaaaaccaa aatttgaac acagtccct 
      gaatggttg cgccgtgaa cacaccaaa attaatgag 
      tttaaagtg aaaagtcag gaacacatc attgacaac 
      ttgcacgca atcctcaaa tcgcaagag tttctcttc 
      aagaatatc atcaaactg gtgaacatc ctcgagaag 
      gatgccctc atcaattca aacagcgaa aaactggac 
      aaaatcaag aggaaactt gtcgcatgc actgatatt 
      ttgtcaaac gcagccact tcgcaacaa ttcacaagc 
      ctcatcaat aaaataatc gaagaagag atcacgcgt 
      tgtagattc aggcacggc gaaagcaaa accagcgct 
      gaaattaat ttaaaagtg ccgaaacct gagggaatc 
      gaagctgct ttggacacg tcccacaag aacaacgac 
      atcagaaga atgaagaaa caactcgct gagaaaatt 
      gcgaaacca aacgtacaa aataaacag aatgagcgt 
      ttaaggggc cgtattatc gacctggaa gtgaaaaca 
      aattcggcc aagttacta aaacgggcg agatccgag 
      ccagttttg aacgaaaac cgaaaattg gaaaccatc 
      tggaagaaa acacagatt gagattgaa gaatccaaa 
      aaagtgata caagtcaat gaagaccgt ttcaagaaa 
      tttgaaaac acactttgg cacacctta acaaccatg 
      aaatttttg tcgctgaaa agtgattac gcaaatatg 
      accaagtgt gcgttctat aataagaga cagtttgtg 
      gcgacggaa gcaacaatt tcgaaaaaa gtccaagga 
      gaggaaaag ttcggcaag tttgttaag ataagtaac 
      tccatgttc cacattacg gaaaaacgt ctgtcgttt 
      gaggaggga caattagat ggtgttgga gaaagtgga 
      ggagcaaat aataaaaag acagaagtg tctaaagac 
      aagattttt ccaaaaaaa tctgttagt aatgctcca 
      cccgtgctt atacaaaac cagaaaaaa gacgaacag 
      ggcaataat accattata aatggcaat gacatcaaa 
      aaattggtt cagaaccat tccgcaaat gccactgtt 
      gcggtagtt gttgcagga ggtgacaaa agtgtgatc 
      aagtctcga gaaatagta ccaacggaa cgagacaac 
      aaacaaact aacacaatt attagtgtt cataatttt 
      attaagaaa acaggcgag gtttctaaa aatgttgca 
      cctttagat gcaattaag aaagatgat cagaaaact 
      attctggag aaagaagga acaagtaaa gctttaaat 
      cccattaga acagaagat gagaggaaa actactcca 
      cagaaaaat ccacaaaaa actgaggag caacaaatt 
      gttaaaagt aaagataat gaacccaaa tttgctgta 
      cccgaaaaa atagaaaga atgaatcaa caaacaaca 
      gatcagcgt ttcgtacag tcgttaaga aattcggac 
      ttgaataag aagccttgc aaaacaact agaaatgtt 
      aaaatgagg ttgaatgtc atcctaaaa acgaaggat 
      tctttggga acaaagcgc actacagct ccacctccg 
      ttaattaag agttctaaa aaagaatcc ttatttaac 
      aatgttgga gcaaacaca aaactccaa actattgcc 
      agcgataat ttaaaattg tgcactttg aacgcagtt 
      tctcctaaa aaagctaaa gacgaaagc aaaaaagtg 
      tccatgcac gaatttaaa agcgaggct gatctcatc 
      catatgaac aataaagca tttcaggaa ctagaaact 
      acaattaaa acggttcag attgttaaa ccaaatgga 
      aaaacacaa aatgttaag aagcaggag aaaaaaagt 
      gtgtctagt gaaaatttg gcatcgatt atgaaacag 
      cggaatgat attaaacgc gaaatgaaa tccgaggcg 
      tcttcgcga gctggaggt gatgagaaa ataagccgc 
      aaaaataag cgtgatttg catacagaa gtcaagcaa 
      ctgtctaaa gaaaaatgt aaaacgttt ttgaaaact 
      tcgaggata aaatcgttc gtgaaacca aaccccagt 
      aatcaacaa atgcgccgt acgacgaaa aatgcgatg 
      aagagacag caagttttg acaatttta cctaaagtt 
      gcgaaagaa attaaagag aaaaaaaag ccgcaagtt 
      tgtttggtt gaacccggg gaaataaaa accgtgaaa 
      atgaaagac agttattgc gtgtccaaa aagtcaagt 
      tacgtgaaa ttttctaaa aaaaatttg cccaacaaa 
      aaaattaat cagacgagt tgggaagaa gaggatagg 
      tacaaggtt gcctcagtg gtggaagac taatctgtt 
      taagtcttg tcttgtgta aaaacgtgc aagtgaaat 
      aaattgggt tttgataaa acatattat ttatttttt 
      atataataa aaaacttaa acctatttc acacttaac 
      aaatttgca ctaagtctt tacaagcca tttgagtta 
      ctttgcgac agcggtcgc ctaaacagt aaaatgtgt 
      ggttcaggc gtgtgcaac atgtaatgt acccaattg 
      gggctttgt tgtaccccc aaattgcgc cattctgtt 
      tcggtcatt aggtgggcc cggggcacg agtttggcc 
      aaatcggcg ggtaaaatc acgtgccgg tactcgtag 
      tgctcgtcg gagtacttc tccgagtac aaaatgtta 
      tttaccggc atttttacg ctacaaatt gatttgttt 
      tgattgaca taacctcaa aaccggcaa taaaaacgt 
      accagtgct gtaaaaaac taaaataat gtactgcta 
      cttgaagca agcaattaa tacactttt ttaaattcg 
      ttaaactcg tcactggtg gttcaaaat tattgttaa 
      atgataatt taacgttat tgtttgaat tac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY