Status
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Gene information
General information
TC007149 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8577909..8602020
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007149
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04285

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    prothorax musculature partially lateralnot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna proximal size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    mesothorax circumference decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007149 RA
      MACCLVAAW TTLLWLASA TGLDPTGLL SSATSPDQS 
      IDLLGALGS HNSSWAGVT LAQGPQQIR PAYYLQGDY 
      RDLKLPAAA FQQVSDLLR RSPEFTISA WLRQEVGNT 
      GSIVSFAHG LNRYLELQS SGRKNEIRL HYTSRVDSK 
      VYVETFHYR LADNLWHHV AVSVSGSQA ELLVDCHPL 
      YKRLLRPGA PDRNFTEPQ QLWLGQRNK HYHFKGAMQ 
      DVRLIGGPH GYLSLCPSL DSTCPTCGQ FSLLQNTVQ 
      ELTRHLQEL SERLVAAEG RISKVEECD CQKSCHVNG 
      TVHADGATW QRGCDLCAC VHGEVQCRP VECPELPCK 
      HPVNISGEC CQSCLRHCL FYGAFYDHG ELVTIKKCV 
      ECECQDGSM QCTRIDPET MCPKLTCPP DEQFSVADN 
      CCKFCPGVD YCAKGHVCH ANATCLNLQ TTYACQCDQ 
      GFQGDGRLC TDINECQQE GGLEGHHCH QNTRCVNTP 
      GSYTCECLA GYRRVDKFN CAELDECSS GEHNCDVNA 
      DCINTQGSY HCVCKEGYT GDGYNCQPV CNQSCLNGG 
      ICRSPGKCA CPNGYTGSS CERDLDECA TNAHRCTEA 
      SVCVNMIGW YYCKCKEGY QSPVSDNNL GTLCQDIDE 
      CVDELHTCH PTAQCVNVN GGFRCECPP GKEDCRLSC 
      MFEGTELAH GLSLSPQKD PCQTCTCNY GVITCDKPH 
      CNCSLPGSS QNPCCPQCD KKHACKHQE LTDVVLMHG 
      EQWSYQCQT CECLYGEVD CWEMKCPPL LCDNPVQAI 
      GDCCPHCDD LCPLGNAST WGQPCTFAG RMYESGAQF 
      ADPNDPCVS CNCKDGVLC CSYNYHCGN DTHAVGFDG 
      VVASTGGAK GHSGGGTGE ASAVSDFTK QYSAALRVA 
      NSSAGVVYV SDSMGGNKT EQSPPGG
    CDS sequence
    • >TC007149 RA
      atggcctgc tgcttggtc gcggcttgg accaccctg 
      ctgtggctt gcctcagct accggcttg gaccctacg 
      ggtctgctg tcatctgcc acgtctcca gatcaatca 
      atcgacctc ctgggtgcc ttgggttca cacaacagt 
      agttgggca ggcgttaca ctggcgcaa ggacctcaa 
      caaatccgg ccggcttac tacttacaa ggagactac 
      cgggacctg aagctgccg gcggccgct ttccagcag 
      gtgtcggac ctgctgcgt cgcagtccc gagttcacg 
      atttcggcg tggctgcgg caagaagtg ggcaacacg 
      ggtagcata gtgagtttc gcacacggc ttaaacaga 
      tacttagaa ctgcaatcg agcggccgg aaaaacgaa 
      atccggctg cactatacc tcgcgggtg gactctaaa 
      gtgtacgtg gaaacgttc cattacaga ctcgcggac 
      aatctgtgg catcatgtg gctgtgtcg gtgagtggt 
      tcgcaagcg gaactgcta gtggattgc catccgctc 
      tataaaaga ctcttgagg cccggtgct ccggaccgg 
      aacttcacc gaacctcaa cagctctgg ctaggccag 
      aggaacaag cattaccac tttaaggga gccatgcag 
      gacgttcgt ctgatagga ggtccccac ggttacctg 
      tcgctgtgc cccagcctg gactcgacg tgtcccacg 
      tgtggccag ttctcgttg ctgcagaac accgtgcag 
      gagctgaca aggcatctt caggaactt tccgaaagg 
      ttggtagca gcagaggga cgtatcagt aaagtggag 
      gaatgtgac tgtcagaaa tcgtgccac gtcaacggg 
      acggtgcat gcggacggc gccacgtgg caacggggc 
      tgtgattta tgtgcttgc gtgcacgga gaagtacaa 
      tgtaggccc gtcgagtgc ccggagctg ccctgcaaa 
      catccagtc aacatcagc ggagagtgt tgccaaagt 
      tgtcttaga cattgtcta ttttatggt gctttttac 
      gaccatgga gagctagtg accatcaag aagtgtgtg 
      gagtgtgag tgtcaagac ggcagcatg cagtgcacc 
      agaatagat ccagagacg atgtgtccc aagctcacc 
      tgtcctcca gacgaacaa ttcagcgtc gcagataac 
      tgttgcaag ttttgtcca ggtgttgac tattgtgca 
      aaagggcat gtttgccat gctaacgca acctgcctc 
      aacttacaa actacttac gcttgtcag tgcgaccaa 
      ggctttcaa ggggatgga cgattgtgc actgatatc 
      aacgagtgc cagcaagag ggcggcctc gaaggccac 
      cactgccac caaaacacg cgctgcgtc aacaccccc 
      ggatcttac acctgtgag tgcctcgcc ggttaccga 
      agagtggac aaatttaat tgcgcggaa ttggatgag 
      tgcagctcg ggcgaacac aactgcgac gtcaacgcc 
      gattgcatc aacacccaa ggcagttat cactgcgtt 
      tgcaaggag gggtacaca ggagacggc tacaactgc 
      cagcctgtt tgtaaccaa agttgcctc aacggtgga 
      atctgcagg agtccgggc aaatgcgcg tgtcctaac 
      ggctacacc ggctccagc tgcgagcga gacttggac 
      gaatgcgcc accaacgcc catcgatgc accgaagct 
      tcggtttgc gtgaacatg atcggttgg tactattgt 
      aaatgtaaa gaggggtac cagagcccc gtctcagac 
      aataattta ggaacttta tgccaagat atcgacgaa 
      tgtgtggac gaattgcac acttgtcac ccgactgct 
      caatgcgtg aatgtcaac ggaggcttc cgatgcgag 
      tgtcctccc gggaaggaa gactgccgc ctaagctgc 
      atgttcgaa ggcactgaa ctagcccac ggcttgtcc 
      ctatctccg cagaaagac ccttgccag acgtgcact 
      tgcaactac ggagtgatc acctgcgac aagccgcac 
      tgcaactgc tcgcttcct ggcagtagc caaaacccc 
      tgttgtccc cagtgcgac aaaaaacac gcctgcaag 
      caccaggag ctcaccgat gtggtcctc atgcacgga 
      gagcaatgg tcgtaccaa tgccagact tgcgagtgt 
      ctctacggc gaagttgac tgctgggag atgaaatgt 
      ccgccgttg ttatgtgac aatcccgtg caagccatc 
      ggggactgc tgtccccac tgtgacgat ttgtgtcct 
      ttggggaac gcgtccacg tgggggcag ccgtgcact 
      tttgcggga agaatgtac gaatcgggg gcgcaattt 
      gccgacccc aacgatccc tgcgtctcc tgtaactgc 
      aaggacgga gtgctttgc tgcagttat aattaccat 
      tgcggtaac gatacgcac gcagtgggc tttgacgga 
      gtggtggcc tccacgggc ggtgcgaag ggacactct 
      ggtggtggc accggcgag gcgtcggca gtcagtgac 
      tttactaaa caatatagt gcagctctt cgagtggct 
      aatagtagt gctggggtc gtctacgtc agtgactcg 
      atggggggt aacaagact gaacaatcg ccgccaggg 
      ggctga
    mRNA sequence
    • >TC007149 RA
      tatcggacg tctcgacgc gggcgccgg cgcggacag 
      ccgtccaaa tccttcggc agcgaactg agctcagtc 
      tctgacggt ctttcggtg agaagtagc gtcgcgcga 
      tatcctgct gcctagtgc tgaccatgt gtattattg 
      ctggccgct gccctcgct cgtgatcaa cccttcgag 
      tgctgtgac ggctcccgt catgggccg gcttcccaa 
      gggctgaca attctgtga ggggccgcg gagagaggc 
      catggcctg ctgcttggt cgcggcttg gaccaccct 
      gctgtggct tgcctcagc taccggctt ggaccctac 
      gggtctgct gtcatctgc cacgtctcc agatcaatc 
      aatcgacct cctgggtgc cttgggttc acacaacag 
      tagttgggc aggcgttac actggcgca aggacctca 
      acaaatccg gccggctta ctacttaca aggagacta 
      ccgggacct gaagctgcc ggcggccgc tttccagca 
      ggtgtcgga cctgctgcg tcgcagtcc cgagttcac 
      gatttcggc gtggctgcg gcaagaagt gggcaacac 
      gggtagcat agtgagttt cgcacacgg cttaaacag 
      atacttaga actgcaatc gagcggccg gaaaaacga 
      aatccggct gcactatac ctcgcgggt ggactctaa 
      agtgtacgt ggaaacgtt ccattacag actcgcgga 
      caatctgtg gcatcatgt ggctgtgtc ggtgagtgg 
      ttcgcaagc ggaactgct agtggattg ccatccgct 
      ctataaaag actcttgag gcccggtgc tccggaccg 
      gaacttcac cgaacctca acagctctg gctaggcca 
      gaggaacaa gcattacca ctttaaggg agccatgca 
      ggacgttcg tctgatagg aggtcccca cggttacct 
      gtcgctgtg ccccagcct ggactcgac gtgtcccac 
      gtgtggcca gttctcgtt gctgcagaa caccgtgca 
      ggagctgac aaggcatct tcaggaact ttccgaaag 
      gttggtagc agcagaggg acgtatcag taaagtgga 
      ggaatgtga ctgtcagaa atcgtgcca cgtcaacgg 
      gacggtgca tgcggacgg cgccacgtg gcaacgggg 
      ctgtgattt atgtgcttg cgtgcacgg agaagtaca 
      atgtaggcc cgtcgagtg cccggagct gccctgcaa 
      acatccagt caacatcag cggagagtg ttgccaaag 
      ttgtcttag acattgtct attttatgg tgcttttta 
      cgaccatgg agagctagt gaccatcaa gaagtgtgt 
      ggagtgtga gtgtcaaga cggcagcat gcagtgcac 
      cagaataga tccagagac gatgtgtcc caagctcac 
      ctgtcctcc agacgaaca attcagcgt cgcagataa 
      ctgttgcaa gttttgtcc aggtgttga ctattgtgc 
      aaaagggca tgtttgcca tgctaacgc aacctgcct 
      caacttaca aactactta cgcttgtca gtgcgacca 
      aggctttca aggggatgg acgattgtg cactgatat 
      caacgagtg ccagcaaga gggcggcct cgaaggcca 
      ccactgcca ccaaaacac gcgctgcgt caacacccc 
      cggatctta cacctgtga gtgcctcgc cggttaccg 
      aagagtgga caaatttaa ttgcgcgga attggatga 
      gtgcagctc gggcgaaca caactgcga cgtcaacgc 
      cgattgcat caacaccca aggcagtta tcactgcgt 
      ttgcaagga ggggtacac aggagacgg ctacaactg 
      ccagcctgt ttgtaacca aagttgcct caacggtgg 
      aatctgcag gagtccggg caaatgcgc gtgtcctaa 
      cggctacac cggctccag ctgcgagcg agacttgga 
      cgaatgcgc caccaacgc ccatcgatg caccgaagc 
      ttcggtttg cgtgaacat gatcggttg gtactattg 
      taaatgtaa agaggggta ccagagccc cgtctcaga 
      caataattt aggaacttt atgccaaga tatcgacga 
      atgtgtgga cgaattgca cacttgtca cccgactgc 
      tcaatgcgt gaatgtcaa cggaggctt ccgatgcga 
      gtgtcctcc cgggaagga agactgccg cctaagctg 
      catgttcga aggcactga actagccca cggcttgtc 
      cctatctcc gcagaaaga cccttgcca gacgtgcac 
      ttgcaacta cggagtgat cacctgcga caagccgca 
      ctgcaactg ctcgcttcc tggcagtag ccaaaaccc 
      ctgttgtcc ccagtgcga caaaaaaca cgcctgcaa 
      gcaccagga gctcaccga tgtggtcct catgcacgg 
      agagcaatg gtcgtacca atgccagac ttgcgagtg 
      tctctacgg cgaagttga ctgctggga gatgaaatg 
      tccgccgtt gttatgtga caatcccgt gcaagccat 
      cggggactg ctgtcccca ctgtgacga tttgtgtcc 
      tttggggaa cgcgtccac gtgggggca gccgtgcac 
      ttttgcggg aagaatgta cgaatcggg ggcgcaatt 
      tgccgaccc caacgatcc ctgcgtctc ctgtaactg 
      caaggacgg agtgctttg ctgcagtta taattacca 
      ttgcggtaa cgatacgca cgcagtggg ctttgacgg 
      agtggtggc ctccacggg cggtgcgaa gggacactc 
      tggtggtgg caccggcga ggcgtcggc agtcagtga 
      ctttactaa acaatatag tgcagctct tcgagtggc 
      taatagtag tgctggggt cgtctacgt cagtgactc 
      gatgggggg taacaagac tgaacaatc gccgccagg 
      gggctgagg gcccccgct cgaggaccg gtctaacat 
      tcctggaca cttgcgagc cacttgtgc aatagccta 
      cggccactc tgagtgttc tgtctgccg agctaggct 
      gcttgctag gcctcatcc caagtgtct tcctagtac 
      cgtagccag gtttcagcc taggcttcg ccagcttta 
      catttgtta tgatgatgt tacactaaa cacattcaa 
      aacggatgc tggactact agaatgtgg caactccaa 
      acccgttca agtattcac attcggcgc ctaactgat 
      gttatatat caaaagatg tatatttat caaagttta 
      atatcaaaa aactatatt atactatag tacaatcac 
      aatcgtttt aagcgcaag ttgtagcat aattattat 
      aaaattgtt cgaatttta tggcttttg ctttgaggt 
      gcgttttaa acacaggtg tcagcattt tagtagtcc 
      gttcagtaa tgtctgttt ctgtttctt gagcgtaat 
      taccaaaac tctagtcaa atgaacgat gggcttgct 
      agaaatgta ctacgcaag ctttacata ttaacaact 
      aaattaagt aatggaagc atatctagc tacttaaat 
      ttattgcaa tattaaaat tgtgatatt gttaaatga 
      gactaataa ttaatgtaa cttttccat taaatgtaa 
      attagttcc tgcgacatg tgcgctgtg tagcgctcc 
      aaccaaatt tggggtgtc acttttgag caaatatta 
      aatattaaa gaaaaaaca tgtacttag cacaaagta 
      aatattacg ataagatag gctaatcaa acaccatca 
      aatttttaa tagtgtaaa attaattat accgctgaa 
      caacgaact gtttaactg tttcttgat ttgtaacaa 
      tattcacta gataagtaa cttgtaaat tatttaatg 
      ctcattatt tacgtatat agcgcagta cctattttg 
      tcgatattt tttttacca aagatgtca ctgttgtac 
      ataaaatat gattgcaga agtaatgta cttttagaa 
      aagtgaaaa atagttgag actttaatt gagctaaag 
      tttttcaag tcaagcaca tactctttg tcacacgtc 
      agtattttt tatttacgt cagttactc tcattatta 
      tcattggct tcaaagtta cattcttaa gcaaacatg 
      ctgtaattt tcatttaat ttgttacaa agttactaa 
      aacataact accgactat tatgtatat ttcgagatt 
      tatatttat ttacaaaca tgttattat tgtacagac 
      gtcgccata atccttctg ttctaaaga atgtgtgcc 
      aatcattat tacaaataa attaaacaa ataaacatg 
      tcctatttc a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY