Status
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Gene information
General information
TC007147 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8613479..8632720
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007147
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04284

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007147 RA
      MMMMMDYGS QYASSTMSS ETLSGSCGS RTNRIRTVR 
      LVRPTHGTL PPPSLTCRH GPSLGFSVR GGREHGTGF 
      FVSHVEPAS EAHRQGLRV GDQIIRVNG FTVDDAVHK 
      EVLQLISNH THLTLKVRS VGMIPVKDK KTDTLSWQI 
      ITDNSSLRS SPPLGEKIH DVRINIIVA PRSKLGCGI 
      CKGPEWKPG IFIQFTKEG GIAREAGLR PGDQILFCN 
      NVDFSDIPF NEAVNLMKT SRQLDLIVR KSAGSELFP 
      GESSGYNSS ASSVTGDQS PSWSDQKRL SIVKEESLD 
      LEDRLDRLK SKKWEKIGW DDGESEEKQ YFKPTIINL 
      SENGTTIKN NGTEEECQS IDNASRNKL AELCLVSQQ 
      HETKTVVVE VHRSNTEQE NKTENTLVK SSSTSSFGS 
      LASRSSTAS SSLSSAICQ EIQRRSEKK QIEQDKPSI 
      DEQLQMKKI LKGVTSEKQ SQHNKLMDE FKKAHRKMF 
      KAPDNEDKT DSAAPAVGK ETLDRIAGK ELSQKLSER 
      LSNLSMQES LQDRLNNRN STTTTPPPP PPPPIAETT 
      NGTPNTNTI KSNKKGKAP PVPVKTSTL SYQQPPPCP 
      TPDYDTLSI TSTTSLPKQ NGYKPSQSH LNDSVEMES 
      LESFKLNNP GGNKPKPPN TYFKMSNGT TSSTSTIRK 
      QRPVSVTIG EYPSGTMRK TPGRLDFLQ NGTETSPSP 
      TNTPISNQL ASELAQTLN RSNLRKRTE SMENLLNCP 
      QIKPQSNGS VRISLNTSL AKSLAKSTN DLTNLENFS 
      KAFANRVTI NVNTGDKKT DTPNGILKN GTHKPLMQQ 
      KSITFGEMP TVINDRPLH VS
    CDS sequence
    • >TC007147 RA
      atgatgatg atgatggat tatggttcg caatacgct 
      tcctcgacc atgtcttcg gagacgctt tcgggaagc 
      tgtgggagc agaactaat cggattcgt accgtgagg 
      ctcgtccgc cccacgcac ggcaccctg ccgcctccg 
      agcttgacc tgtcgacac ggccccagc ttggggttc 
      agcgtgagg ggcggccgc gagcatggg acggggttc 
      ttcgtgtcg cacgtggag cccgcctcc gaggcccat 
      cgacaggga ctaagggtc ggagaccaa atcatcaga 
      gtgaacgga ttcaccgtc gatgatgcc gtccacaaa 
      gaagtcctc caattgatt tccaaccac acgcatctc 
      accttgaaa gtgagaagt gtcggaatg attcccgtg 
      aaagacaag aaaaccgat actctcagc tggcaaatc 
      atcacggac aattcctct ttgaggtct tcaccgccg 
      ctgggagaa aaaatacac gacgttaga atcaacatc 
      attgtggca ccgaggtcc aagctcggc tgcgggatt 
      tgcaaaggt ccggagtgg aagcctggc atcttcatt 
      cagtttaca aaagagggc ggaatagca cgcgaggct 
      ggccttcga cccggcgat cagatcctc ttttgcaac 
      aacgtggac tttagcgac atacctttt aacgaggct 
      gtaaattta atgaaaaca tcgcgccag ctcgattta 
      atagtccgt aaatctgcc ggatcggag ctttttcca 
      ggggagtcc agtgggtat aatagttca gcgagctcg 
      gtgacggga gaccagagc ccgtcgtgg tctgaccag 
      aaacgactt tccatagtt aaagaagaa tctctcgac 
      ttggaagac cgacttgac cgactcaag tcgaaaaaa 
      tgggaaaag attggatgg gacgacggt gaaagtgaa 
      gagaaacag tactttaag ccaaccatc atcaatcta 
      tccgagaac gggacgacc ataaaaaac aacggaact 
      gaagaagag tgccaaagc atcgataac gcgagcaga 
      aacaaactc gcggagctt tgcctcgtc tcgcagcag 
      cacgagacg aaaaccgtc gtcgtcgaa gtgcacaga 
      agtaacact gagcaagaa aacaagact gaaaacact 
      ttggtcaag agttcatcg accagcagt ttcggtagt 
      ttggctagc aggagcagc acagcctcg agcagctta 
      tctagcgct atctgtcaa gaaatacaa aggagatct 
      gaaaagaag caaatcgag caagacaag ccgtccatc 
      gacgagcaa ctccaaatg aagaaaatc ctcaaagga 
      gtgacatcg gagaagcag agccagcat aataagctc 
      atggacgag ttcaaaaaa gcccaccgg aagatgttc 
      aaagctccc gacaacgaa gataaaacg gattccgcc 
      gcacctgcc gttgggaaa gaaactctg gatcgtatc 
      gctggtaaa gaattgtcg cagaaatta tcagaacga 
      ttatcaaac ctttccatg caagaaagt ttgcaagac 
      cgactgaac aaccggaat tccacaaca acgacgccg 
      cccccacca ccaccaccg ccaatcgca gaaacaacc 
      aacggtact cccaacacc aacacaatt aaatcgaat 
      aaaaagggc aaagcgccc ccggttccc gtcaaaact 
      tccacactg tcgtaccag cagccgccc ccatgtccc 
      acccccgac tatgacacc ctcagcatc acatcgaca 
      acttcccta ccaaagcag aacggatac aaacctagt 
      caaagccat ttaaacgat tcagtcgaa atggaatcc 
      ttggaatcc ttcaagctc aataacccg ggcgggaac 
      aagcccaaa ccccccaac acatacttc aaaatgtcg 
      aatggcacc acctccagc acctccacg atccgcaaa 
      cagcgccct gtcagcgtc accatcgga gagtacccc 
      tcggggacc atgcggaag accccaggc cggctggac 
      ttcctccaa aacggcacc gaaaccagc ccatccccc 
      accaacacc ccaatttcc aaccaactg gcctccgag 
      ctcgcccaa accctaaac cggtccaac ctgcggaaa 
      cgcactgaa tcgatggaa aatttgcta aattgtccg 
      caaattaaa ccgcaaagt aatggatcc gtgcgcatc 
      tcgcttaac actagcctt gccaaaagc ctggccaag 
      tccacgaac gacttgacc aacttggag aacttctcc 
      aaggctttt gcaaaccgc gttacgatt aacgttaat 
      accggggat aagaagacg gacacaccc aacggaatt 
      ttaaaaaat ggcacgcat aaaccgctg atgcagcag 
      aaaagtatt acgtttggg gaaatgcca acggtgata 
      aatgaccgt ccactacat gtgtcctaa
    mRNA sequence
    • >TC007147 RA
      ggagctttt gcgcaagcc cgtcacgaa gaacaggtg 
      aaacaccac acatcatca aacaggtat aacgaataa 
      acacacgca gctccggag tgcctgacc atagacgaa 
      agaaggggc aacagagca tggaatcgg tgaaggcaa 
      caactcttc caacacaca atgtctagt gctcgcttg 
      ccgcacatt gcccgaaga cttcaactg tgaagccga 
      tagataaac agtgaactt gtggtgctt ctggtgcgt 
      gtttccggt attgataaa acagtgaag cccggagtt 
      attcaccta acagtgaac ttttgttaa gctcggttt 
      tggacagag acgatatga caggatttg ctatttaac 
      gctgataac tgaagagcc atgatgatg atgatggat 
      tatggttcg caatacgct tcctcgacc atgtcttcg 
      gagacgctt tcgggaagc tgtgggagc agaactaat 
      cggattcgt accgtgagg ctcgtccgc cccacgcac 
      ggcaccctg ccgcctccg agcttgacc tgtcgacac 
      ggccccagc ttggggttc agcgtgagg ggcggccgc 
      gagcatggg acggggttc ttcgtgtcg cacgtggag 
      cccgcctcc gaggcccat cgacaggga ctaagggtc 
      ggagaccaa atcatcaga gtgaacgga ttcaccgtc 
      gatgatgcc gtccacaaa gaagtcctc caattgatt 
      tccaaccac acgcatctc accttgaaa gtgagaagt 
      gtcggaatg attcccgtg aaagacaag aaaaccgat 
      actctcagc tggcaaatc atcacggac aattcctct 
      ttgaggtct tcaccgccg ctgggagaa aaaatacac 
      gacgttaga atcaacatc attgtggca ccgaggtcc 
      aagctcggc tgcgggatt tgcaaaggt ccggagtgg 
      aagcctggc atcttcatt cagtttaca aaagagggc 
      ggaatagca cgcgaggct ggccttcga cccggcgat 
      cagatcctc ttttgcaac aacgtggac tttagcgac 
      atacctttt aacgaggct gtaaattta atgaaaaca 
      tcgcgccag ctcgattta atagtccgt aaatctgcc 
      ggatcggag ctttttcca ggggagtcc agtgggtat 
      aatagttca gcgagctcg gtgacggga gaccagagc 
      ccgtcgtgg tctgaccag aaacgactt tccatagtt 
      aaagaagaa tctctcgac ttggaagac cgacttgac 
      cgactcaag tcgaaaaaa tgggaaaag attggatgg 
      gacgacggt gaaagtgaa gagaaacag tactttaag 
      ccaaccatc atcaatcta tccgagaac gggacgacc 
      ataaaaaac aacggaact gaagaagag tgccaaagc 
      atcgataac gcgagcaga aacaaactc gcggagctt 
      tgcctcgtc tcgcagcag cacgagacg aaaaccgtc 
      gtcgtcgaa gtgcacaga agtaacact gagcaagaa 
      aacaagact gaaaacact ttggtcaag agttcatcg 
      accagcagt ttcggtagt ttggctagc aggagcagc 
      acagcctcg agcagctta tctagcgct atctgtcaa 
      gaaatacaa aggagatct gaaaagaag caaatcgag 
      caagacaag ccgtccatc gacgagcaa ctccaaatg 
      aagaaaatc ctcaaagga gtgacatcg gagaagcag 
      agccagcat aataagctc atggacgag ttcaaaaaa 
      gcccaccgg aagatgttc aaagctccc gacaacgaa 
      gataaaacg gattccgcc gcacctgcc gttgggaaa 
      gaaactctg gatcgtatc gctggtaaa gaattgtcg 
      cagaaatta tcagaacga ttatcaaac ctttccatg 
      caagaaagt ttgcaagac cgactgaac aaccggaat 
      tccacaaca acgacgccg cccccacca ccaccaccg 
      ccaatcgca gaaacaacc aacggtact cccaacacc 
      aacacaatt aaatcgaat aaaaagggc aaagcgccc 
      ccggttccc gtcaaaact tccacactg tcgtaccag 
      cagccgccc ccatgtccc acccccgac tatgacacc 
      ctcagcatc acatcgaca acttcccta ccaaagcag 
      aacggatac aaacctagt caaagccat ttaaacgat 
      tcagtcgaa atggaatcc ttggaatcc ttcaagctc 
      aataacccg ggcgggaac aagcccaaa ccccccaac 
      acatacttc aaaatgtcg aatggcacc acctccagc 
      acctccacg atccgcaaa cagcgccct gtcagcgtc 
      accatcgga gagtacccc tcggggacc atgcggaag 
      accccaggc cggctggac ttcctccaa aacggcacc 
      gaaaccagc ccatccccc accaacacc ccaatttcc 
      aaccaactg gcctccgag ctcgcccaa accctaaac 
      cggtccaac ctgcggaaa cgcactgaa tcgatggaa 
      aatttgcta aattgtccg caaattaaa ccgcaaagt 
      aatggatcc gtgcgcatc tcgcttaac actagcctt 
      gccaaaagc ctggccaag tccacgaac gacttgacc 
      aacttggag aacttctcc aaggctttt gcaaaccgc 
      gttacgatt aacgttaat accggggat aagaagacg 
      gacacaccc aacggaatt ttaaaaaat ggcacgcat 
      aaaccgctg atgcagcag aaaagtatt acgtttggg 
      gaaatgcca acggtgata aatgaccgt ccactacat 
      gtgtcctaa cgctcggag ccggccttt ggaggagtg 
      caatcgtga accaagtgc aaatatctt atgtctcta 
      agctgaata ttaatccta tgtattgtg aaagtgtgg 
      cgagttgat tgtgataaa ggcatattc ggtatgagt 
      atcataata atcctgaaa taactctgt agttgatca 
      tattcgtat gtatgtatg tcatgtaaa ctgtcaata 
      atgtgtatt ttaatctga cgtaacgta cagtgcaaa 
      ataaagtcc taatcataa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY