Status
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Gene information
General information
TC007127 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8939928..8943218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007127
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04280

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007127 RA
      MIGYKALVL CVFFNLARA VQFGSVPLE EVLHVLVPT 
      LNSDNPQCR NDTIFYVEE LKKLRLWAT EMFDASSKF 
      PTGLLSGST YDFGNFDEC VEVSGPVTE EKIQGRYCM 
      AKFHIAPPD FKHEEQKYA RFFNGDNYF INASTWEKM 
      IAFSNKRSR NTRNEFFFS YCIPSSCTY DDLESSLKQ 
      VTAKLNSFS DFKVTASVN PRNCQLKPE LKFSGADIG 
      FTCVVLFFI LLACIATSY DWLIKLDDL KKYRLEGKK 
      HKVLMSFSL WSNLKKLSH ETENDDGLQ CIHGLKVIS 
      MFLIIMGHR IMFGIGSPL MNPSFVENM YGRIEATAL 
      LNGPVIVDT FFNISGFLV CYLMLTQLE KRKRGINFL 
      FLYLHRFIR LTPAYAMVL AFYCTLFVK MGSGPLWLE 
      RVQVEQERC VASWWANLF YLNNYINKE HICMFQSWY 
      MTCDMHLFM ISPFIIWLL RKKPFLGLV SLFVLIVAS 
      ISVVFATVY LSKEDAILM LYMKFLRDP VINNTFKNI 
      YIPTHMRAS PYFVGMVTG YIKYVMKSR EYKMPKYMV 
      YMGWVMSIF IIEVTVYSA FIFYLPDKP YDPIISAIY 
      SAMHHFTWS ICISWIVIA ISEGYGAWV QPVLEWKPM 
      ILLSRITYA AFLCHGAIQ IYSVAILRH ANYAGVFHL 
      VFYTAGDIT LAYIAGLLL SMLFESPIL GLEKIILGR 
      VNPSSDKEE TCKVSKVVT PKIKIINQF
    CDS sequence
    • >TC007127 RA
      atgatcgga tacaaagct ctagttttg tgcgttttc 
      ttcaacttg gcccgagct gtccaattc ggaagcgtg 
      cctctggaa gaagttttg cacgttttg gtaccgacg 
      ctcaattcg gacaacccc cagtgccga aatgacacg 
      attttttac gtcgaagag ttgaaaaaa ttgagactg 
      tgggcgacg gaaatgttt gatgcttcg tccaaattt 
      cccaccggc cttttgtcg ggctcgacc tacgatttt 
      ggaaatttt gacgaatgc gtcgaagtg agtggacct 
      gtgaccgag gaaaaaata caggggagg tactgtatg 
      gccaaattt cacatcgca ccacccgac ttcaagcat 
      gaggaacaa aaatacgca cgttttttt aacggagat 
      aattatttt attaatgcc tccacgtgg gaaaaaatg 
      attgctttt tcgaataaa aggtctcgc aacactagg 
      aacgaattt ttcttttcg tactgcatc ccatcgtct 
      tgcacttac gacgatctg gaatcttcg ctgaaacaa 
      gtcactgcg aagttgaat tctttcagc gatttcaaa 
      gtcacggca agtgtaaat ccgcggaat tgtcaactt 
      aaacctgaa ttaaaattc tccggtgct gatattggt 
      tttacgtgc gtggtcctg ttttttatt cttttagca 
      tgtattgcc acgtcctac gactggtta ataaaactg 
      gacgattta aaaaaatat cgacttgag gggaaaaaa 
      cataaagtt ttaatgtcg ttctcctta tggtcaaac 
      ctgaaaaaa ctaagccat gaaactgaa aatgacgat 
      gggcttcag tgcatccac ggcctgaaa gtaatttcc 
      atgttctta attatcatg ggacacaga atcatgttc 
      gggattggg tcaccgcta atgaacccg agcttcgtc 
      gaaaacatg tacggccga attgaagcg accgctctc 
      ctcaacggc cctgtgata gtcgacacc tttttcaac 
      atttccgga tttttggtt tgttattta atgctaacc 
      caattagag aagcgaaaa cgcggcatc aactttctc 
      ttcctctac ctccaccgt tttataaga ctgacccct 
      gcctatgct atggtcttg gctttttat tgtaccctc 
      tttgtaaaa atgggttcc ggccctttg tggctcgaa 
      agggttcaa gtggaacaa gagcgttgc gttgctagt 
      tggtgggcc aatttattt tatttaaat aattacatc 
      aacaaggaa cacatttgt atgtttcag tcgtggtac 
      atgacatgc gatatgcac ctgtttatg atttcacct 
      ttcataatt tggttatta aggaaaaaa ccttttcta 
      ggcctggtg tctctcttt gtccttatt gtagcctca 
      atctctgtt gtttttgcc accgtatat ctcagtaaa 
      gaggacgca attctaatg ctttacatg aagtttttg 
      cgagatcct gtgattaat aacacgttc aaaaacatt 
      tacatcccg actcacatg agggccagc ccttacttt 
      gtcgggatg gttacggga tatattaaa tatgttatg 
      aagtctcgg gagtataaa atgcccaaa tatatggtt 
      tacatggga tgggttatg agtattttc attattgaa 
      gtcactgtt tactccgcc tttattttc tacttgccg 
      gataagcct tacgaccct attatttcc gcaatttat 
      tccgcaatg catcacttc acctggagc atttgcatc 
      agttggatt gtcattgca atttcggag ggatacgga 
      gcttgggtt cagccagtc cttgaatgg aaacctatg 
      atccttctc agcagaata acatacgcg gcgttcctt 
      tgccatgga gccatccag atttactca gtcgcgatt 
      ctaaggcac gcaaattat gccggcgtt tttcatctc 
      gttttctac actgctggt gatataacg ttggcgtac 
      attgccggc ctactttta agcatgttg ttcgagtcc 
      cctattcta ggactagag aaaattatt ttaggcaga 
      gtgaaccca tcgagtgac aaggaggaa acttgcaag 
      gtctcaaaa gttgtaacc ccaaaaatc aaaataatt 
      aatcaattt taa
    mRNA sequence
    • >TC007127 RA
      gtggtatta ataatcctt ttcaggaac aaacaagtc 
      catctcaac aagtgccca gtgctagtt tttcaccac 
      tttacgaag agctggtta ttctcaagg tgactctag 
      ctgaagtga ccttcccgt ctgaaattc gtcagtctc 
      tagtccttt atagcgaag cgttgctga agcttttcg 
      tccatttgc aagaattat gtcacaatg atcggatac 
      aaagctcta gttttgtgc gttttcttc aacttggcc 
      cgagctgtc caattcgga agcgtgcct ctggaagaa 
      gttttgcac gttttggta ccgacgctc aattcggac 
      aacccccag tgccgaaat gacacgatt ttttacgtc 
      gaagagttg aaaaaattg agactgtgg gcgacggaa 
      atgtttgat gcttcgtcc aaatttccc accggcctt 
      ttgtcgggc tcgacctac gattttgga aattttgac 
      gaatgcgtc gaagtgagt ggacctgtg accgaggaa 
      aaaatacag gggaggtac tgtatggcc aaatttcac 
      atcgcacca cccgacttc aagcatgag gaacaaaaa 
      tacgcacgt ttttttaac ggagataat tattttatt 
      aatgcctcc acgtgggaa aaaatgatt gctttttcg 
      aataaaagg tctcgcaac actaggaac gaatttttc 
      ttttcgtac tgcatccca tcgtcttgc acttacgac 
      gatctggaa tcttcgctg aaacaagtc actgcgaag 
      ttgaattct ttcagcgat ttcaaagtc acggcaagt 
      gtaaatccg cggaattgt caacttaaa cctgaatta 
      aaattctcc ggtgctgat attggtttt acgtgcgtg 
      gtcctgttt tttattctt ttagcatgt attgccacg 
      tcctacgac tggttaata aaactggac gatttaaaa 
      aaatatcga cttgagggg aaaaaacat aaagtttta 
      atgtcgttc tccttatgg tcaaacctg aaaaaacta 
      agccatgaa actgaaaat gacgatggg cttcagtgc 
      atccacggc ctgaaagta atttccatg ttcttaatt 
      atcatggga cacagaatc atgttcggg attgggtca 
      ccgctaatg aacccgagc ttcgtcgaa aacatgtac 
      ggccgaatt gaagcgacc gctctcctc aacggccct 
      gtgatagtc gacaccttt ttcaacatt tccggattt 
      ttggtttgt tatttaatg ctaacccaa ttagagaag 
      cgaaaacgc ggcatcaac tttctcttc ctctacctc 
      caccgtttt ataagactg acccctgcc tatgctatg 
      gtcttggct ttttattgt accctcttt gtaaaaatg 
      ggttccggc cctttgtgg ctcgaaagg gttcaagtg 
      gaacaagag cgttgcgtt gctagttgg tgggccaat 
      ttattttat ttaaataat tacatcaac aaggaacac 
      atttgtatg tttcagtcg tggtacatg acatgcgat 
      atgcacctg tttatgatt tcacctttc ataatttgg 
      ttattaagg aaaaaacct tttctaggc ctggtgtct 
      ctctttgtc cttattgta gcctcaatc tctgttgtt 
      tttgccacc gtatatctc agtaaagag gacgcaatt 
      ctaatgctt tacatgaag tttttgcga gatcctgtg 
      attaataac acgttcaaa aacatttac atcccgact 
      cacatgagg gccagccct tactttgtc gggatggtt 
      acgggatat attaaatat gttatgaag tctcgggag 
      tataaaatg cccaaatat atggtttac atgggatgg 
      gttatgagt attttcatt attgaagtc actgtttac 
      tccgccttt attttctac ttgccggat aagccttac 
      gaccctatt atttccgca atttattcc gcaatgcat 
      cacttcacc tggagcatt tgcatcagt tggattgtc 
      attgcaatt tcggaggga tacggagct tgggttcag 
      ccagtcctt gaatggaaa cctatgatc cttctcagc 
      agaataaca tacgcggcg ttcctttgc catggagcc 
      atccagatt tactcagtc gcgattcta aggcacgca 
      aattatgcc ggcgttttt catctcgtt ttctacact 
      gctggtgat ataacgttg gcgtacatt gccggccta 
      cttttaagc atgttgttc gagtcccct attctagga 
      ctagagaaa attatttta ggcagagtg aacccatcg 
      agtgacaag gaggaaact tgcaaggtc tcaaaagtt 
      gtaacccca aaaatcaaa ataattaat caattttaa 
      attaaataa ataagtctt ggttgtaca agatacttg 
      taagtctga ttttcgtga ttaataaat tattttaag 
      cagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY