| General information | |||
| TC032893
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 9048808..9073768 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032893 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04279 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence -
Penetrance (percentage of animals/eggs on slide): 30% - 50%
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect, flour poorly removed,
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect, flour poorly removed,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen posterior partiallyremaining - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide -
Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen posterior partiallyremaining - Penetrance (percentage of animals/eggs on slide): 30% - 50%
| Comment: some head appendages are mostly attached to it |
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 7 (16 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
Days post injection
- >TC032893 RA
MPAVRIAQD ADGAGTDTH QDILTCGVC QKPFALSDI VRFIQHKVA SCNKENFGQ CFAATDRER DNDDGALPL STINTRRPS ISAPISGKK ASGNRVHTP PPASPRLPA PGDLCVDGA ASSTPKRRA SASPLTSSS MEDGEDIKP DIKQERMDT TEESSQCKK SRTEFADAE SNTTHSEQC RKKRFRSDH RAHHYDITS AVSRLTSRD VLSYPCFLV IVKGAPGSD FQLPFASPD TIGSVIETD LPFFVPEPS NYVCSTCKA RLHSAWRLI QHVQHSHGI KIYIESSPT SSKNNNHST SSASSSSSG CSSGGPPPP PSMRHHLLP PPDLHNPFG VGGLLRLPL PPLNHGPVP PAPLFSRPE HHYRIDQLV SEQFRHHGL NLAAAAAAV AAQGVPPPH ASFPSPAER APSASSVPS RERSTPNQP LSLEPQLDF YSQRLRQLA GTTSPGAAQ ASSPSPRKH SPPFASPSP SQLPPTSIP NNISSSSSS SNNNNNNNN SSNSGGSRA TSTSPPAKD DNTQLTATP RSASTPPGK PGSPRNSNS GEAVHACEY CGKKFRYQN NLDAHRRTH TGELPYKCT VCDHACAQS SKLKRHMKI HRTPEDRTS NAGSVETLD GDESEEDDE VDEEEEEED ELEEEEAED LSVPTQIPP KNANQSASL VGELMDKFG LSNIAQYSE AYKQALQES NSALKLQLA SKDRDNNNS TSLTNQLKL RDEFAKSLL PGPPPQPLP LFPPFNDTY EATKRLKLD LERSDWWLP GLPRENKGL PGPSALLPN PLIKKDTNR RNDTCEFCG KVFKNCSNL TVHRRSHTG EKPYKCELC SYACAQSSK LTRHMKTHG RIGKDVYRC RFCEMPFSV PSTLEKHMR KCVVNQGKG HLLSTMPML SGDEDSSAS IASKEAT
- >TC032893 RB
MPAVRIAQA DADGAGTDT HQDILTCGV CQKPFALSD IVRFIQHKV ASCNKENFG QCFAATDRE RDNDDGALP LSTINTRRP SISAPISGK KASGNRVHT PPPASPRLP APGDLCVDG AASSTPKRR ASASPLTSS SMEDGEDIK PDIKQERMD TTEESSQCK KSRTEFADA ESNTTHSEQ CRKKRFRSD HRAHHYDIT SAVSRLTSR DVLSYPCFL VIVKGAPGS DFQLPFASP DTIGSVIET DLPFFVPEP SNYVCSTCK ARLHSAWRL IQHVQHSHG IKIYIESSP TSSKNNNHS TSSASSSSS GCSSGGPPP PPSMRHHLL PPPDLHNPF GVGGLLRLP LPPLNHGPV PPAPLFSRP EHHYRIDQL VSEQFRHHG LNLAAAAAA VAAQGVPPP HASFPSPAE RAPSASSVP SRERSTPNQ PLSLEPQLD FYSQRLRQL AGTTSPGAA QASSPSPRK HSPPFASPS PSQLPPTSI PNNISSSSS SSNNNNNNN NSSNSGGSR ATSTSPPAK DDNTQLTAT PRSASTPPG KPGSPRNSN SGEAVHACE YCGKKFRYQ NNLDAHRRT HTGELPYKC TVCDHACAQ SSKLKRHMK IHRTPEDRT SNAGSVETL DGDESEEDD EVDEEEEEE DELEEEEAE DLSVPTQIP PKNANQSAS LVGELMDKF GLSNIAQYS EAYKQALQE SNSALKLQL ASKDRDNNN STSLTNQLK LRDEFAKSL LPGPPPQPL PLFPPFNDT YEATKRLKL DLERSDWWL PGLPRENKG LPGPSALLP NPLIKKDTN RRNDTCEFC GKVFKNCSN LTVHRRSHT GEKPYKCEL CSYACAQSS KLTRHMKTH GRIGKDVYR CRFCEMPFS VPSTLEKHM RKCVVNQGK GHLLSTMPM LSGDEDSSA SIASKEAT
- >TC032893 RC
MEETDADGA GTDTHQDIL TCGVCQKPF ALSDIVRFI QHKVASCNK ENFGQCFAA TDRERDNDD GALPLSTIN TRRPSISAP ISGKKASGN RVHTPPPAS PRLPAPGDL CVDGAASST PKRRASASP LTSSSMEDG EDIKPDIKQ ERMDTTEES SQCKKSRTE FADAESNTT HSEQCRKKR FRSDHRAHH YDITSAVSR LTSRDVLSY PCFLVIVKG APGSDFQLP FASPDTIGS VIETDLPFF VPEPSNYVC STCKARLHS AWRLIQHVQ HSHGIKIYI ESSPTSSKN NNHSTSSAS SSSSGCSSG GPPPPPSMR HHLLPPPDL HNPFGVGGL LRLPLPPLN HGPVPPAPL FSRPEHHYR IDQLVSEQF RHHGLNLAA AAAAVAAQG VPPPHASFP SPAERAPSA SSVPSRERS TPNQPLSLE PQLDFYSQR LRQLAGTTS PGAAQASSP SPRKHSPPF ASPSPSQLP PTSIPNNIS SSSSSSNNN NNNNNSSNS GGSRATSTS PPAKDDNTQ LTATPRSAS TPPGKPGSP RNSNSGEAV HACEYCGKK FRYQNNLDA HRRTHTGEL PYKCTVCDH ACAQSSKLK RHMKIHRTP EDRTSNAGS VETLDGDES EEDDEVDEE EEEEDELEE EEAEDLSVP TQIPPKNAN QSASLVGEL MDKFGLSNI AQYSEAYKQ ALQESNSAL KLQLASKDR DNNNSTSLT NQLKLRDEF AKSLLPGPP PQPLPLFPP FNDTYEATK RLKLDLERS DWWLPGLPR ENKGLPGPS ALLPNPLIK KDTNRRNDT CEFCGKVFK NCSNLTVHR RSHTGEKPY KCELCSYAC AQSSKLTRH MKTHGRIGK DVYRCRFCE MPFSVPSTL EKHMRKCVV NQGKGHLLS TMPMLSGDE DSSASIASK EAT
- >TC032893 RA
atgccagca gtgcgtata gcgcaagac gcagacggg gcgggcaca gacacccac caggacatc ctgacttgc ggcgtgtgc cagaagcct ttcgccctc tccgacatc gtccgcttc atccagcac aaagtagcg tcttgcaac aaagaaaac ttcggacaa tgtttcgcc gccaccgac cgagaacga gacaacgac gacggggca ctgcccctt tccaccatc aacacccgc cggccgagc atctcggcc cccatctcg gggaaaaag gcgtctggg aacagggtg cacactccg ccgccagcc tcccccaga ctgccggca cctggcgat ctgtgcgtc gacggcgcc gcctcctcc acccccaag cggagggcc tcggcgtcg ccgctcacc tcctccagc atggaggac ggcgaggac atcaagccc gacatcaag caggagcgg atggacacc acggaggag agcagccag tgcaagaaa tcgcgaacg gagttcgca gacgccgag tccaacact actcatagt gaacaatgc agaaagaag cgatttcgg tccgatcac cgagcccat cattacgat attacttcc gccgtctca agattgacc tcgcgagat gtgctcagt tatccgtgc ttcctcgtc attgtcaaa ggagcgcca ggatcggac ttccagctc ccatttgcc agtccggac actatcgga tcagtgatt gagactgat ctccctttc tttgttcca gaaccgagt aactacgtt tgttcaacg tgcaaagcg aggctgcac tcggcctgg cgcctaatc cagcacgtc cagcactcc catggaatc aaaatctac atcgaaagc tcccccacc agcagcaag aacaacaac cacagtacg agcagtgct agtagtagc tcgtcggga tgctcctct ggcgggcct ccgccgccg cccagtatg cgccaccac ttgctgccg ccccccgac ttgcacaac ccctttggt gttggcggt ttgctgcgg ttaccgctc ccgccactt aaccacggt ccagttcct ccagcgccg cttttttcc cgcccggaa caccactac cgcatcgat caactcgtt agcgaacag ttccgacac catggcttg aatctggcg gcggcagcg gcggcggtg gccgcccaa ggcgtccct cccccacat gcgagtttt ccatcacct gcagaacgg gcaccttct gcgtctagt gttccgtca agggaacgc agcacgccc aaccaaccc ctttcatta gaaccacaa ctggatttt tactcgcag cggttgagg caactcgct ggtacaacc agtccggga gcggctcag gcgagctcg ccgtctcca cggaagcac tcaccgcca ttcgccagt ccctcgcca tcgcagctg ccacccacg agtatacca aacaacatc agcagcagt agcagcagc agcaataac aacaacaac aataacaat agtagcaat agtggcgga agtagagcc acgagtacg tcacctcca gccaaagat gataacaca caactaaca gcaactcca aggtcggct tcaactcca ccaggaaaa cccggatct cctcgaaat tcaaactca ggagaagca gtacatgcg tgcgagtac tgtggcaag aaattcagg taccagaat aatttagac gcccatcga agaacacat acaggggaa ttaccgtac aaatgcacg gtttgtgat catgcatgt gcccaaagt tcaaaactt aaacgccac atgaaaata catcgaaca ccagaagat cgtaccagc aatgccggt tctgtggaa acacttgat ggtgatgaa agtgaagaa gatgacgaa gtggatgaa gaagaagag gaagaagac gagttggag gaggaagaa gctgaagat ttatctgtt cctacacaa atacctcca aaaaacgcc aaccaatcg gcttcacta gtcggggaa ctgatggat aagttcggt ttgagcaac atcgcgcaa tacagtgaa gcttacaag caagcgctt caagaatca aactcggcc cttaaactc caactcgcg agtaaagac cgcgataac aacaacagc accagcttg accaaccaa ctgaaactt cgcgacgag ttcgccaaa agcctcctt cccggtcct ccccctcag ccattgcct ctgttcccg ccgttcaac gatacgtac gaagccacc aaacggctg aaactcgac ttggaacgc agcgactgg tggctgccg ggcttgccg cgcgaaaac aagggcctc cccggcccc agtgccctc ctccctaac cccctaatc aaaaaagat acgaacaga cggaacgat acttgtgag ttctgtggc aaagtgttt aagaactgt tcgaatttg acggtgcac cggcgctcg cacacgggc gaaaagccg tacaagtgc gagctgtgt tcctacgct tgtgcgcaa agttcgaaa ttgacgcgg catatgaaa acgcacggt cggatcggg aaggacgtc taccggtgc aggttttgc gagatgccg ttttcggtg ccgtcgacg ctagagaaa cacatgcgg aagtgtgtg gtgaaccag gggaagggt cacttgctg tcgacgatg cccatgctg agcggagac gaggactcg tcagcgagc atcgcctcc aaggaggca acatga
- >TC032893 RB
atgccagca gtgcgtata gcgcaagca gacgcagac ggggcgggc acagacacc caccaggac atcctgact tgcggcgtg tgccagaag cctttcgcc ctctccgac atcgtccgc ttcatccag cacaaagta gcgtcttgc aacaaagaa aacttcgga caatgtttc gccgccacc gaccgagaa cgagacaac gacgacggg gcactgccc ctttccacc atcaacacc cgccggccg agcatctcg gcccccatc tcggggaaa aaggcgtct gggaacagg gtgcacact ccgccgcca gcctccccc agactgccg gcacctggc gatctgtgc gtcgacggc gccgcctcc tccaccccc aagcggagg gcctcggcg tcgccgctc acctcctcc agcatggag gacggcgag gacatcaag cccgacatc aagcaggag cggatggac accacggag gagagcagc cagtgcaag aaatcgcga acggagttc gcagacgcc gagtccaac actactcat agtgaacaa tgcagaaag aagcgattt cggtccgat caccgagcc catcattac gatattact tccgccgtc tcaagattg acctcgcga gatgtgctc agttatccg tgcttcctc gtcattgtc aaaggagcg ccaggatcg gacttccag ctcccattt gccagtccg gacactatc ggatcagtg attgagact gatctccct ttctttgtt ccagaaccg agtaactac gtttgttca acgtgcaaa gcgaggctg cactcggcc tggcgccta atccagcac gtccagcac tcccatgga atcaaaatc tacatcgaa agctccccc accagcagc aagaacaac aaccacagt acgagcagt gctagtagt agctcgtcg ggatgctcc tctggcggg cctccgccg ccgcccagt atgcgccac cacttgctg ccgcccccc gacttgcac aaccccttt ggtgttggc ggtttgctg cggttaccg ctcccgcca cttaaccac ggtccagtt cctccagcg ccgcttttt tcccgcccg gaacaccac taccgcatc gatcaactc gttagcgaa cagttccga caccatggc ttgaatctg gcggcggca gcggcggcg gtggccgcc caaggcgtc cctccccca catgcgagt tttccatca cctgcagaa cgggcacct tctgcgtct agtgttccg tcaagggaa cgcagcacg cccaaccaa cccctttca ttagaacca caactggat ttttactcg cagcggttg aggcaactc gctggtaca accagtccg ggagcggct caggcgagc tcgccgtct ccacggaag cactcaccg ccattcgcc agtccctcg ccatcgcag ctgccaccc acgagtata ccaaacaac atcagcagc agtagcagc agcagcaat aacaacaac aacaataac aatagtagc aatagtggc ggaagtaga gccacgagt acgtcacct ccagccaaa gatgataac acacaacta acagcaact ccaaggtcg gcttcaact ccaccagga aaacccgga tctcctcga aattcaaac tcaggagaa gcagtacat gcgtgcgag tactgtggc aagaaattc aggtaccag aataattta gacgcccat cgaagaaca catacaggg gaattaccg tacaaatgc acggtttgt gatcatgca tgtgcccaa agttcaaaa cttaaacgc cacatgaaa atacatcga acaccagaa gatcgtacc agcaatgcc ggttctgtg gaaacactt gatggtgat gaaagtgaa gaagatgac gaagtggat gaagaagaa gaggaagaa gacgagttg gaggaggaa gaagctgaa gatttatct gttcctaca caaatacct ccaaaaaac gccaaccaa tcggcttca ctagtcggg gaactgatg gataagttc ggtttgagc aacatcgcg caatacagt gaagcttac aagcaagcg cttcaagaa tcaaactcg gcccttaaa ctccaactc gcgagtaaa gaccgcgat aacaacaac agcaccagc ttgaccaac caactgaaa cttcgcgac gagttcgcc aaaagcctc cttcccggt cctccccct cagccattg cctctgttc ccgccgttc aacgatacg tacgaagcc accaaacgg ctgaaactc gacttggaa cgcagcgac tggtggctg ccgggcttg ccgcgcgaa aacaagggc ctccccggc cccagtgcc ctcctccct aacccccta atcaaaaaa gatacgaac agacggaac gatacttgt gagttctgt ggcaaagtg tttaagaac tgttcgaat ttgacggtg caccggcgc tcgcacacg ggcgaaaag ccgtacaag tgcgagctg tgttcctac gcttgtgcg caaagttcg aaattgacg cggcatatg aaaacgcac ggtcggatc gggaaggac gtctaccgg tgcaggttt tgcgagatg ccgttttcg gtgccgtcg acgctagag aaacacatg cggaagtgt gtggtgaac caggggaag ggtcacttg ctgtcgacg atgcccatg ctgagcgga gacgaggac tcgtcagcg agcatcgcc tccaaggag gcaacatga
- >TC032893 RC
atggaagaa acagacgca gacggggcg ggcacagac acccaccag gacatcctg acttgcggc gtgtgccag aagcctttc gccctctcc gacatcgtc cgcttcatc cagcacaaa gtagcgtct tgcaacaaa gaaaacttc ggacaatgt ttcgccgcc accgaccga gaacgagac aacgacgac ggggcactg cccctttcc accatcaac acccgccgg ccgagcatc tcggccccc atctcgggg aaaaaggcg tctgggaac agggtgcac actccgccg ccagcctcc cccagactg ccggcacct ggcgatctg tgcgtcgac ggcgccgcc tcctccacc cccaagcgg agggcctcg gcgtcgccg ctcacctcc tccagcatg gaggacggc gaggacatc aagcccgac atcaagcag gagcggatg gacaccacg gaggagagc agccagtgc aagaaatcg cgaacggag ttcgcagac gccgagtcc aacactact catagtgaa caatgcaga aagaagcga tttcggtcc gatcaccga gcccatcat tacgatatt acttccgcc gtctcaaga ttgacctcg cgagatgtg ctcagttat ccgtgcttc ctcgtcatt gtcaaagga gcgccagga tcggacttc cagctccca tttgccagt ccggacact atcggatca gtgattgag actgatctc cctttcttt gttccagaa ccgagtaac tacgtttgt tcaacgtgc aaagcgagg ctgcactcg gcctggcgc ctaatccag cacgtccag cactcccat ggaatcaaa atctacatc gaaagctcc cccaccagc agcaagaac aacaaccac agtacgagc agtgctagt agtagctcg tcgggatgc tcctctggc gggcctccg ccgccgccc agtatgcgc caccacttg ctgccgccc cccgacttg cacaacccc tttggtgtt ggcggtttg ctgcggtta ccgctcccg ccacttaac cacggtcca gttcctcca gcgccgctt ttttcccgc ccggaacac cactaccgc atcgatcaa ctcgttagc gaacagttc cgacaccat ggcttgaat ctggcggcg gcagcggcg gcggtggcc gcccaaggc gtccctccc ccacatgcg agttttcca tcacctgca gaacgggca ccttctgcg tctagtgtt ccgtcaagg gaacgcagc acgcccaac caacccctt tcattagaa ccacaactg gatttttac tcgcagcgg ttgaggcaa ctcgctggt acaaccagt ccgggagcg gctcaggcg agctcgccg tctccacgg aagcactca ccgccattc gccagtccc tcgccatcg cagctgcca cccacgagt ataccaaac aacatcagc agcagtagc agcagcagc aataacaac aacaacaat aacaatagt agcaatagt ggcggaagt agagccacg agtacgtca cctccagcc aaagatgat aacacacaa ctaacagca actccaagg tcggcttca actccacca ggaaaaccc ggatctcct cgaaattca aactcagga gaagcagta catgcgtgc gagtactgt ggcaagaaa ttcaggtac cagaataat ttagacgcc catcgaaga acacataca ggggaatta ccgtacaaa tgcacggtt tgtgatcat gcatgtgcc caaagttca aaacttaaa cgccacatg aaaatacat cgaacacca gaagatcgt accagcaat gccggttct gtggaaaca cttgatggt gatgaaagt gaagaagat gacgaagtg gatgaagaa gaagaggaa gaagacgag ttggaggag gaagaagct gaagattta tctgttcct acacaaata cctccaaaa aacgccaac caatcggct tcactagtc ggggaactg atggataag ttcggtttg agcaacatc gcgcaatac agtgaagct tacaagcaa gcgcttcaa gaatcaaac tcggccctt aaactccaa ctcgcgagt aaagaccgc gataacaac aacagcacc agcttgacc aaccaactg aaacttcgc gacgagttc gccaaaagc ctccttccc ggtcctccc cctcagcca ttgcctctg ttcccgccg ttcaacgat acgtacgaa gccaccaaa cggctgaaa ctcgacttg gaacgcagc gactggtgg ctgccgggc ttgccgcgc gaaaacaag ggcctcccc ggccccagt gccctcctc cctaacccc ctaatcaaa aaagatacg aacagacgg aacgatact tgtgagttc tgtggcaaa gtgtttaag aactgttcg aatttgacg gtgcaccgg cgctcgcac acgggcgaa aagccgtac aagtgcgag ctgtgttcc tacgcttgt gcgcaaagt tcgaaattg acgcggcat atgaaaacg cacggtcgg atcgggaag gacgtctac cggtgcagg ttttgcgag atgccgttt tcggtgccg tcgacgcta gagaaacac atgcggaag tgtgtggtg aaccagggg aagggtcac ttgctgtcg acgatgccc atgctgagc ggagacgag gactcgtca gcgagcatc gcctccaag gaggcaaca tga
- >TC032893 RA
catttcgtc accttatct cagtggttg acacaattt caacataat taagaggct aagtgtata tttgctcgg gggtattat tagattagg gctcaggcg aagacgtgc tgtttgagc accgctccg tagtgtttt ttgcgcgag ctttcgctc cggagcgct ccggtgtag catccggag agccagcgg gcaaagccc ccactccgg gcgcgtata tgcgcagca gcatccctt cctatgggg tgagcacgg ccggtggct agtgagtgg ccttcccga gcccgaacg atacgaacc gactaacac cgacccaga aacggccgc tctgtcgcc gaaagcgtc cgcgaacac gacacacgc gctcggacc attggccac tcagcatct ctggtccag gaccggaca tgggccccc gaccccgag tgacatgag acgagttcg cttagtagt gccccaggt gtagtgacc cagtgacag tgatgagac tctgcctct gagagacta acaccgcca agatgagaa tcaagatgc cagcagtgc gtatagcgc aagacgcag acggggcgg gcacagaca cccaccagg acatcctga cttgcggcg tgtgccaga agcctttcg ccctctccg acatcgtcc gcttcatcc agcacaaag tagcgtctt gcaacaaag aaaacttcg gacaatgtt tcgccgcca ccgaccgag aacgagaca acgacgacg gggcactgc ccctttcca ccatcaaca cccgccggc cgagcatct cggccccca tctcgggga aaaaggcgt ctgggaaca gggtgcaca ctccgccgc cagcctccc ccagactgc cggcacctg gcgatctgt gcgtcgacg gcgccgcct cctccaccc ccaagcgga gggcctcgg cgtcgccgc tcacctcct ccagcatgg aggacggcg aggacatca agcccgaca tcaagcagg agcggatgg acaccacgg aggagagca gccagtgca agaaatcgc gaacggagt tcgcagacg ccgagtcca acactactc atagtgaac aatgcagaa agaagcgat ttcggtccg atcaccgag cccatcatt acgatatta cttccgccg tctcaagat tgacctcgc gagatgtgc tcagttatc cgtgcttcc tcgtcattg tcaaaggag cgccaggat cggacttcc agctcccat ttgccagtc cggacacta tcggatcag tgattgaga ctgatctcc ctttctttg ttccagaac cgagtaact acgtttgtt caacgtgca aagcgaggc tgcactcgg cctggcgcc taatccagc acgtccagc actcccatg gaatcaaaa tctacatcg aaagctccc ccaccagca gcaagaaca acaaccaca gtacgagca gtgctagta gtagctcgt cgggatgct cctctggcg ggcctccgc cgccgccca gtatgcgcc accacttgc tgccgcccc ccgacttgc acaacccct ttggtgttg gcggtttgc tgcggttac cgctcccgc cacttaacc acggtccag ttcctccag cgccgcttt tttcccgcc cggaacacc actaccgca tcgatcaac tcgttagcg aacagttcc gacaccatg gcttgaatc tggcggcgg cagcggcgg cggtggccg cccaaggcg tccctcccc cacatgcga gttttccat cacctgcag aacgggcac cttctgcgt ctagtgttc cgtcaaggg aacgcagca cgcccaacc aaccccttt cattagaac cacaactgg atttttact cgcagcggt tgaggcaac tcgctggta caaccagtc cgggagcgg ctcaggcga gctcgccgt ctccacgga agcactcac cgccattcg ccagtccct cgccatcgc agctgccac ccacgagta taccaaaca acatcagca gcagtagca gcagcagca ataacaaca acaacaata acaatagta gcaatagtg gcggaagta gagccacga gtacgtcac ctccagcca aagatgata acacacaac taacagcaa ctccaaggt cggcttcaa ctccaccag gaaaacccg gatctcctc gaaattcaa actcaggag aagcagtac atgcgtgcg agtactgtg gcaagaaat tcaggtacc agaataatt tagacgccc atcgaagaa cacatacag gggaattac cgtacaaat gcacggttt gtgatcatg catgtgccc aaagttcaa aacttaaac gccacatga aaatacatc gaacaccag aagatcgta ccagcaatg ccggttctg tggaaacac ttgatggtg atgaaagtg aagaagatg acgaagtgg atgaagaag aagaggaag aagacgagt tggaggagg aagaagctg aagatttat ctgttccta cacaaatac ctccaaaaa acgccaacc aatcggctt cactagtcg gggaactga tggataagt tcggtttga gcaacatcg cgcaataca gtgaagctt acaagcaag cgcttcaag aatcaaact cggccctta aactccaac tcgcgagta aagaccgcg ataacaaca acagcacca gcttgacca accaactga aacttcgcg acgagttcg ccaaaagcc tccttcccg gtcctcccc ctcagccat tgcctctgt tcccgccgt tcaacgata cgtacgaag ccaccaaac ggctgaaac tcgacttgg aacgcagcg actggtggc tgccgggct tgccgcgcg aaaacaagg gcctccccg gccccagtg ccctcctcc ctaaccccc taatcaaaa aagatacga acagacgga acgatactt gtgagttct gtggcaaag tgtttaaga actgttcga atttgacgg tgcaccggc gctcgcaca cgggcgaaa agccgtaca agtgcgagc tgtgttcct acgcttgtg cgcaaagtt cgaaattga cgcggcata tgaaaacgc acggtcgga tcgggaagg acgtctacc ggtgcaggt tttgcgaga tgccgtttt cggtgccgt cgacgctag agaaacaca tgcggaagt gtgtggtga accagggga agggtcact tgctgtcga cgatgccca tgctgagcg gagacgagg actcgtcag cgagcatcg cctccaagg aggcaacat gactctttc cgtggggcg gcatgaggc tcccgccgc cgccgccca gtcttacgt actagtcgc cacgaactc ttagggaat tgtgatcag tgacggaca ctggagggc atcgggaga tcccacgcg tggggtctc ccgatgacg actagctgt gtatagtat tgcgacgag acggccctt ttcgagcga agggccgga tcgacgatt gttcgatgt aaccgatac caaagcaat gtttaactt tcgtaatta ttattgatt attgagttt attattgat tcatgtata tttgtaata attaggttg taggttgtt gtttgagac tgagggccc tggatagcg tgacgtgac gcttcaggc cgtcttggg cccactgta cattcccga tcatatcat ttttgatgg ccagtagcc ttgactgta cataacagt atttattgc cgtgtacta gacccacta tactgtaca aaatgtctc tcattaaat tatttccac ttaatgtat agtattatc attgtatag attcatgtt tttcaaagt tactcttta atgttggtg ttagacaaa tcgagaaat ttgcccctg tgtgtcaac agggtcgtc ttatagatt taattcagg gtcagatta atctatagg agcatatcc gcatttttc ttttacgat tattcctac cgagacaac catcctatt ccgcaaaaa caatccaac aaaaaagaa tgtgatggc tgatcttca ttttgtaat gcaatacta gtaataaca gtggaccag ggtctgttc ttgtagtat ttgccgcaa tgccggatt caattcaat gtaactgta actattaat gttaataat atgttattt cgaatattt ttatttatt tcaacgata tggctgtag cactacggt gttcgtatg ggctgcact acacttaat ataatcgtg ttcctata
- >TC032893 RB
catttcgtc accttatct cagtggttg acacaattt caacataat taagaggct aagtgtata tttgctcgg gggtattat tagattagg gctcaggcg aagacgtgc tgtttgagc accgctccg tagtgtttt ttgcgcgag ctttcgctc cggagcgct ccggtgtag catccggag agccagcgg gcaaagccc ccactccgg gcgcgtata tgcgcagca gcatccctt cctatgggg tgagcacgg ccggtggct agtgagtgg ccttcccga gcccgaacg atacgaacc gactaacac cgacccaga aacggccgc tctgtcgcc gaaagcgtc cgcgaacac gacacacgc gctcggacc attggccac tcagcatct ctggtccag gaccggaca tgggccccc gaccccgag tgacatgag acgagttcg cttagtagt gccccaggt gtagtgacc cagtgacag tgatgagac tctgcctct gagagacta acaccgcca agatgagaa tcaagatgc cagcagtgc gtatagcgc aagcagacg cagacgggg cgggcacag acacccacc aggacatcc tgacttgcg gcgtgtgcc agaagcctt tcgccctct ccgacatcg tccgcttca tccagcaca aagtagcgt cttgcaaca aagaaaact tcggacaat gtttcgccg ccaccgacc gagaacgag acaacgacg acggggcac tgccccttt ccaccatca acacccgcc ggccgagca tctcggccc ccatctcgg ggaaaaagg cgtctggga acagggtgc acactccgc cgccagcct cccccagac tgccggcac ctggcgatc tgtgcgtcg acggcgccg cctcctcca cccccaagc ggagggcct cggcgtcgc cgctcacct cctccagca tggaggacg gcgaggaca tcaagcccg acatcaagc aggagcgga tggacacca cggaggaga gcagccagt gcaagaaat cgcgaacgg agttcgcag acgccgagt ccaacacta ctcatagtg aacaatgca gaaagaagc gatttcggt ccgatcacc gagcccatc attacgata ttacttccg ccgtctcaa gattgacct cgcgagatg tgctcagtt atccgtgct tcctcgtca ttgtcaaag gagcgccag gatcggact tccagctcc catttgcca gtccggaca ctatcggat cagtgattg agactgatc tccctttct ttgttccag aaccgagta actacgttt gttcaacgt gcaaagcga ggctgcact cggcctggc gcctaatcc agcacgtcc agcactccc atggaatca aaatctaca tcgaaagct cccccacca gcagcaaga acaacaacc acagtacga gcagtgcta gtagtagct cgtcgggat gctcctctg gcgggcctc cgccgccgc ccagtatgc gccaccact tgctgccgc cccccgact tgcacaacc cctttggtg ttggcggtt tgctgcggt taccgctcc cgccactta accacggtc cagttcctc cagcgccgc ttttttccc gcccggaac accactacc gcatcgatc aactcgtta gcgaacagt tccgacacc atggcttga atctggcgg cggcagcgg cggcggtgg ccgcccaag gcgtccctc ccccacatg cgagttttc catcacctg cagaacggg caccttctg cgtctagtg ttccgtcaa gggaacgca gcacgccca accaacccc tttcattag aaccacaac tggattttt actcgcagc ggttgaggc aactcgctg gtacaacca gtccgggag cggctcagg cgagctcgc cgtctccac ggaagcact caccgccat tcgccagtc cctcgccat cgcagctgc cacccacga gtataccaa acaacatca gcagcagta gcagcagca gcaataaca acaacaaca ataacaata gtagcaata gtggcggaa gtagagcca cgagtacgt cacctccag ccaaagatg ataacacac aactaacag caactccaa ggtcggctt caactccac caggaaaac ccggatctc ctcgaaatt caaactcag gagaagcag tacatgcgt gcgagtact gtggcaaga aattcaggt accagaata atttagacg cccatcgaa gaacacata caggggaat taccgtaca aatgcacgg tttgtgatc atgcatgtg cccaaagtt caaaactta aacgccaca tgaaaatac atcgaacac cagaagatc gtaccagca atgccggtt ctgtggaaa cacttgatg gtgatgaaa gtgaagaag atgacgaag tggatgaag aagaagagg aagaagacg agttggagg aggaagaag ctgaagatt tatctgttc ctacacaaa tacctccaa aaaacgcca accaatcgg cttcactag tcggggaac tgatggata agttcggtt tgagcaaca tcgcgcaat acagtgaag cttacaagc aagcgcttc aagaatcaa actcggccc ttaaactcc aactcgcga gtaaagacc gcgataaca acaacagca ccagcttga ccaaccaac tgaaacttc gcgacgagt tcgccaaaa gcctccttc ccggtcctc cccctcagc cattgcctc tgttcccgc cgttcaacg atacgtacg aagccacca aacggctga aactcgact tggaacgca gcgactggt ggctgccgg gcttgccgc gcgaaaaca agggcctcc ccggcccca gtgccctcc tccctaacc ccctaatca aaaaagata cgaacagac ggaacgata cttgtgagt tctgtggca aagtgttta agaactgtt cgaatttga cggtgcacc ggcgctcgc acacgggcg aaaagccgt acaagtgcg agctgtgtt cctacgctt gtgcgcaaa gttcgaaat tgacgcggc atatgaaaa cgcacggtc ggatcggga aggacgtct accggtgca ggttttgcg agatgccgt tttcggtgc cgtcgacgc tagagaaac acatgcgga agtgtgtgg tgaaccagg ggaagggtc acttgctgt cgacgatgc ccatgctga gcggagacg aggactcgt cagcgagca tcgcctcca aggaggcaa catgactct ttccgtggg gcggcatga ggctcccgc cgccgccgc ccagtctta cgtactagt cgccacgaa ctcttaggg aattgtgat cagtgacgg acactggag ggcatcggg agatcccac gcgtggggt ctcccgatg acgactagc tgtgtatag tattgcgac gagacggcc cttttcgag cgaagggcc ggatcgacg attgttcga tgtaaccga taccaaagc aatgtttaa ctttcgtaa ttattattg attattgag tttattatt gattcatgt atatttgta ataattagg ttgtaggtt gttgtttga gactgaggg ccctggata gcgtgacgt gacgcttca ggccgtctt gggcccact gtacattcc cgatcatat catttttga tggccagta gccttgact gtacataac agtatttat tgccgtgta ctagaccca ctatactgt acaaaatgt ctctcatta aattatttc cacttaatg tatagtatt atcattgta tagattcat gtttttcaa agttactct ttaatgttg gtgttagac aaatcgaga aatttgccc ctgtgtgtc aacagggtc gtcttatag atttaattc agggtcaga ttaatctat aggagcata tccgcattt ttcttttac gattattcc taccgagac aaccatcct attccgcaa aaacaatcc aacaaaaaa gaatgtgat ggctgatct tcattttgt aatgcaata ctagtaata acagtggac cagggtctg ttcttgtag tatttgccg caatgccgg attcaattc aatgtaact gtaactatt aatgttaat aatatgtta tttcgaata tttttattt atttcaacg atatggctg tagcactac ggtgttcgt atgggctgc actacactt aatataatc gtgttccta ta
- >TC032893 RC
cgtcgttac ttaatctca gcaaatgca ggcgaagac gtgctgttt gagcaccgc tccgtagtg ttttttgcg cgagctttc gctccggag cgctccggt gtagcatcc ggagagcca gcgggcaaa gcccccact ccgggcgcg tatatgcgc agcagcatc ccttcctat ggggtgagc acggccggt ggctagtga gtggccttc ccgagcccg aacgatacg aaccgacta acaccgacc cagaaacgg ccgctctgt cgccgaaag cgtccgcga acacgacac acgcgctcg gaccattgg ccactcagc atctctggt ccaggaccg gacatgggc ccccgaccc cgagtgaca tgagacgag ttcgcttag tagtgcccc aggtgtagt gacccagtg acagtgatg agactctgc ctctgagag actaacacc gccaagatg agaatcaag atgccagca gtgcgtata gcgcaagct aatcccgtt tggtgctgc taaaggtca gcgtgatgg acacaaatg tcaaatact attcatata tgtcccatt aaattaaca gccttatct tgacatttc ttcaagatt gcattcctc agataattt tcttgcatt acttgttaa cgatgtgtt gccaaaatt tgcagcgtc ttgtatttc gtttaaatt tttaggaaa aaataaatc attcctcaa ccatctgag tttattttt taaatacca aaattccta ataaaatat gcgaattaa gataatgga agaaacaga cgcagacgg ggcgggcac agacaccca ccaggacat cctgacttg cggcgtgtg ccagaagcc tttcgccct ctccgacat cgtccgctt catccagca caaagtagc gtcttgcaa caaagaaaa cttcggaca atgtttcgc cgccaccga ccgagaacg agacaacga cgacggggc actgcccct ttccaccat caacacccg ccggccgag catctcggc ccccatctc ggggaaaaa ggcgtctgg gaacagggt gcacactcc gccgccagc ctcccccag actgccggc acctggcga tctgtgcgt cgacggcgc cgcctcctc cacccccaa gcggagggc ctcggcgtc gccgctcac ctcctccag catggagga cggcgagga catcaagcc cgacatcaa gcaggagcg gatggacac cacggagga gagcagcca gtgcaagaa atcgcgaac ggagttcgc agacgccga gtccaacac tactcatag tgaacaatg cagaaagaa gcgatttcg gtccgatca ccgagccca tcattacga tattacttc cgccgtctc aagattgac ctcgcgaga tgtgctcag ttatccgtg cttcctcgt cattgtcaa aggagcgcc aggatcgga cttccagct cccatttgc cagtccgga cactatcgg atcagtgat tgagactga tctcccttt ctttgttcc agaaccgag taactacgt ttgttcaac gtgcaaagc gaggctgca ctcggcctg gcgcctaat ccagcacgt ccagcactc ccatggaat caaaatcta catcgaaag ctcccccac cagcagcaa gaacaacaa ccacagtac gagcagtgc tagtagtag ctcgtcggg atgctcctc tggcgggcc tccgccgcc gcccagtat gcgccacca cttgctgcc gccccccga cttgcacaa cccctttgg tgttggcgg tttgctgcg gttaccgct cccgccact taaccacgg tccagttcc tccagcgcc gcttttttc ccgcccgga acaccacta ccgcatcga tcaactcgt tagcgaaca gttccgaca ccatggctt gaatctggc ggcggcagc ggcggcggt ggccgccca aggcgtccc tcccccaca tgcgagttt tccatcacc tgcagaacg ggcaccttc tgcgtctag tgttccgtc aagggaacg cagcacgcc caaccaacc cctttcatt agaaccaca actggattt ttactcgca gcggttgag gcaactcgc tggtacaac cagtccggg agcggctca ggcgagctc gccgtctcc acggaagca ctcaccgcc attcgccag tccctcgcc atcgcagct gccacccac gagtatacc aaacaacat cagcagcag tagcagcag cagcaataa caacaacaa caataacaa tagtagcaa tagtggcgg aagtagagc cacgagtac gtcacctcc agccaaaga tgataacac acaactaac agcaactcc aaggtcggc ttcaactcc accaggaaa acccggatc tcctcgaaa ttcaaactc aggagaagc agtacatgc gtgcgagta ctgtggcaa gaaattcag gtaccagaa taatttaga cgcccatcg aagaacaca tacagggga attaccgta caaatgcac ggtttgtga tcatgcatg tgcccaaag ttcaaaact taaacgcca catgaaaat acatcgaac accagaaga tcgtaccag caatgccgg ttctgtgga aacacttga tggtgatga aagtgaaga agatgacga agtggatga agaagaaga ggaagaaga cgagttgga ggaggaaga agctgaaga tttatctgt tcctacaca aatacctcc aaaaaacgc caaccaatc ggcttcact agtcgggga actgatgga taagttcgg tttgagcaa catcgcgca atacagtga agcttacaa gcaagcgct tcaagaatc aaactcggc ccttaaact ccaactcgc gagtaaaga ccgcgataa caacaacag caccagctt gaccaacca actgaaact tcgcgacga gttcgccaa aagcctcct tcccggtcc tccccctca gccattgcc tctgttccc gccgttcaa cgatacgta cgaagccac caaacggct gaaactcga cttggaacg cagcgactg gtggctgcc gggcttgcc gcgcgaaaa caagggcct ccccggccc cagtgccct cctccctaa ccccctaat caaaaaaga tacgaacag acggaacga tacttgtga gttctgtgg caaagtgtt taagaactg ttcgaattt gacggtgca ccggcgctc gcacacggg cgaaaagcc gtacaagtg cgagctgtg ttcctacgc ttgtgcgca aagttcgaa attgacgcg gcatatgaa aacgcacgg tcggatcgg gaaggacgt ctaccggtg caggttttg cgagatgcc gttttcggt gccgtcgac gctagagaa acacatgcg gaagtgtgt ggtgaacca ggggaaggg tcacttgct gtcgacgat gcccatgct gagcggaga cgaggactc gtcagcgag catcgcctc caaggaggc aacatgact ctttccgtg gggcggcat gaggctccc gccgccgcc gcccagtct tacgtacta gtcgccacg aactcttag ggaattgtg atcagtgac ggacactgg agggcatcg ggagatccc acgcgtggg gtctcccga tgacgacta gctgtgtat agtattgcg acgagacgg cccttttcg agcgaaggg ccggatcga cgattgttc gatgtaacc gataccaaa gcaatgttt aactttcgt aattattat tgattattg agtttatta ttgattcat gtatatttg taataatta ggttgtagg ttgttgttt gagactgag ggccctgga tagcgtgac gtgacgctt caggccgtc ttgggccca ctgtacatt cccgatcat atcattttt gatggccag tagccttga ctgtacata acagtattt attgccgtg tactagacc cactatact gtacaaaat gtctctcat taaattatt tccacttaa tgtatagta ttatcattg tatagattc atgtttttc aaagttact ctttaatgt tggtgttag acaaatcga gaaatttgc ccctgtgtg tcaacaggg tcgtcttat agatttaat tcagggtca gattaatct ataggagca tatccgcat ttttctttt acgattatt cctaccgag acaaccatc ctattccgc aaaaacaat ccaacaaaa aagaatgtg atggctgat cttcatttt gtaatgcaa tactagtaa taacagtgg accagggtc tgttcttgt agtatttgc cgcaatgcc ggattcaat tcaatgtaa ctgtaacta ttaatgtta ataatatgt tatttcgaa tatttttat ttatttcaa cgatatggc tgtagcact acggtgttc gtatgggct gcactacac ttaatataa tcgtgttcc tata
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