Status
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Gene information
General information
TC007110 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9250463..9268608
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007110
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04277

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva bent - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007110 RA
      MAHRLIVLM VLWSLASAN NDDWEKKCN KCKCVWSNG 
KRTADCTNR DFSEIPKDL SSEIREIDF SNNPLHYLG 
REVFVNAEL RDIHKLRFV NCSISAMDD TAFKGLVLL 
IELDLSRNS IGLLTSKIF EENRKLRIL SLSHNKVKR 
LDQGLFYNM THLQRLSLD HNEIEYIND SAFFLLPAL 
QHLNLAYNK LTVMSSDFL DNFPKIVSL NLESNPWIC 
DCRLQEFRN QTKRANLIT TQTQCAEPP LLKGRLWTD 
DNIVFACVP QIVEPLPST HIEATTSNI TLTCRVLGD 
PQPDVDWVS NGRIIDRDP RLNTQRFIT SKRKVGDYT 
WNNLTITNV NYRDKGEYK CVAKNPGGS DEKNVSLIV 
SSIGGIGGG GPLAFGATL PLVIALSIG GVIALVVIL 
ILVCCCCRK STHGMATKR RDLQDSSDE CIRLHGQPD 
MEKALITDV NPVMKPPRI CSVPPSVNS GGTEVSEAK 
KNLLDSDSV FAGDDESRS FDFDMPPYR KSQTLLEPD 
YRGNHTYPP DLLPFPPRM CQVSPAGSS ASTVADTSR 
LPAHHGPQS PLHSPLYDS NTLYRTLPY SRSQSPFVG 
PPARVPRQG YVTIPRRPR QRWSTDTPT TSDVEEPLY 
DNLGLRTTV DGSSALSLN KLGEATTPK SIRLFPMSP 
SSCDPIAEN EPPPAAPKL SPSSTQWAK ANAEALRSP 
DNRRNSLPD GGKVKVAPV PPPKPKKRT STGPLFEDE 
GEDGTEV
    CDS sequence
    • >TC007110 RA
      atggcacac cggcttata gtcctgatg gtgctctgg 
agtctggca tctgcgaat aacgacgac tgggaaaaa 
aagtgcaac aagtgcaag tgcgtttgg tcgaacggt 
aaaaggaca gcagattgt acgaatcgc gattttagc 
gaaataccg aaagatttg agttcggaa atccgcgaa 
atagacttt tcgaataac cctttgcat tatttaggc 
cgtgaagtg tttgtgaat gcggaactt agagacatt 
cataagcta aggttcgtt aattgtagt ataagtgca 
atggacgat actgccttc aagggccta gttttacta 
atcgaactg gatttatcg cgcaattca atcgggctt 
ttaacatcg aagatcttc gaagagaac cgcaaatta 
aggatttta tccttgagc cacaataaa gtgaagagg 
ttggaccag gggttgttt tacaatatg acacatctg 
cagagatta tccttagat cataacgaa attgagtac 
atcaatgac tcggcgttt ttccttctc ccagcgctc 
cagcatctc aacttggct tacaacaag ctgacggtg 
atgagttcc gactttttg gacaatttc ccgaaaatt 
gtcagtttg aatctcgaa tccaacccc tggatctgc 
gactgccgc ctccaggag ttccgaaat caaaccaaa 
agggcaaat ttaataacg acccagacc cagtgtgcc 
gagccgccc cttctgaag gggcggtta tggaccgat 
gacaacatc gtcttcgct tgtgtgccc cagatcgtg 
gagccgctc ccaagcacc cacatcgag gccaccacc 
agcaacata accctcact tgccgcgtc ctgggcgac 
ccccagccc gacgtcgac tgggtctcc aacgggcga 
ataatcgac cgagacccc cgactcaac acccaacgc 
ttcatcact tccaaacgc aaagtcgga gactacacg 
tggaataat ttgacaata acaaacgtg aattaccgc 
gacaagggc gaatataaa tgcgtcgcg aaaaatcca 
ggaggttcc gacgagaaa aacgtcagt ttgatcgtc 
tcctccatc ggggggatt gggggaggg ggcccgctg 
gccttcggg gcgaccctc cccctcgtg atagcgttg 
agtatcggc ggagtgatc gccctcgtg gtgatcctc 
atcctcgtg tgctgctgc tgcaggaag agcacgcac 
gggatggcg accaagagg agggacctg caggactcc 
tccgacgag tgtataaga cttcacggg cagccggac 
atggagaag gcgctcatt acggacgtt aaccccgtg 
atgaagccg cccagaatc tgttcagtg cccccgtcg 
gtgaacagc ggaggtacg gaagttagc gaagcaaaa 
aagaatctc ctagacagc gactcggtt tttgcaggc 
gacgacgaa tcccgttcg ttcgacttc gacatgccg 
ccgtaccgc aaatcgcag accctcctg gagccggat 
taccgcggt aatcacacc taccctcca gaccttctt 
cccttcccg ccccgtatg tgccaggtg tccccggcc 
ggtagttcc gcctcgacg gtagcagac acatcccgt 
ttaccagca caccacggc ccacaatcg ccactacac 
agtccactg tacgactcc aacaccctg taccgaact 
cttccttac tccagatcg caatcgccg ttcgtgggc 
ccccccgcc cgagtgccc cgtcagggc tacgtgacg 
ataccccgg cgcccccgg cagcggtgg agcaccgac 
acccccacc acctccgac gtggaggag cccctctac 
gacaacctg gggctgcgc accaccgtg gacggcagc 
tcggctctg tccttgaac aagctgggg gaggccacc 
acccccaag tcgatacgg ctcttcccc atgagcccc 
tccagctgc gaccccatc gcggagaac gagccgccc 
cccgcagcc cccaagctg tctccgtcg agcacgcag 
tgggccaag gcgaacgcc gaggcgttg aggagtccc 
gacaacagg aggaactcg ctcccagat ggagggaag 
gtgaaagta gcgccggtc cctccgcct aaacctaaa 
aaaaggact tcgacggga ccactattc gaagatgaa 
ggtgaagac gggacagaa gtgtga
    mRNA sequence
    • >TC007110 RA
      tatcaatgt tgaaaacgc attgaagct tatttactt 
tgccttttc ttttgcagt tgagggttg caacatgcc 
agacggaat ccgccagca gctctaaat taatataaa 
cgttagcgt atgaagatg gcacaccgg cttatagtc 
ctgatggtg ctctggagt ctggcatct gcgaataac 
gacgactgg gaaaaaaag tgcaacaag tgcaagtgc 
gtttggtcg aacggtaaa aggacagca gattgtacg 
aatcgcgat tttagcgaa ataccgaaa gatttgagt 
tcggaaatc cgcgaaata gacttttcg aataaccct 
ttgcattat ttaggccgt gaagtgttt gtgaatgcg 
gaacttaga gacattcat aagctaagg ttcgttaat 
tgtagtata agtgcaatg gacgatact gccttcaag 
ggcctagtt ttactaatc gaactggat ttatcgcgc 
aattcaatc gggctttta acatcgaag atcttcgaa 
gagaaccgc aaattaagg attttatcc ttgagccac 
aataaagtg aagaggttg gaccagggg ttgttttac 
aatatgaca catctgcag agattatcc ttagatcat 
aacgaaatt gagtacatc aatgactcg gcgtttttc 
cttctccca gcgctccag catctcaac ttggcttac 
aacaagctg acggtgatg agttccgac tttttggac 
aatttcccg aaaattgtc agtttgaat ctcgaatcc 
aacccctgg atctgcgac tgccgcctc caggagttc 
cgaaatcaa accaaaagg gcaaattta ataacgacc 
cagacccag tgtgccgag ccgcccctt ctgaagggg 
cggttatgg accgatgac aacatcgtc ttcgcttgt 
gtgccccag atcgtggag ccgctccca agcacccac 
atcgaggcc accaccagc aacataacc ctcacttgc 
cgcgtcctg ggcgacccc cagcccgac gtcgactgg 
gtctccaac gggcgaata atcgaccga gacccccga 
ctcaacacc caacgcttc atcacttcc aaacgcaaa 
gtcggagac tacacgtgg aataatttg acaataaca 
aacgtgaat taccgcgac aagggcgaa tataaatgc 
gtcgcgaaa aatccagga ggttccgac gagaaaaac 
gtcagtttg atcgtctcc tccatcggg gggattggg 
ggagggggc ccgctggcc ttcggggcg accctcccc 
ctcgtgata gcgttgagt atcggcgga gtgatcgcc 
ctcgtggtg atcctcatc ctcgtgtgc tgctgctgc 
aggaagagc acgcacggg atggcgacc aagaggagg 
gacctgcag gactcctcc gacgagtgt ataagactt 
cacgggcag ccggacatg gagaaggcg ctcattacg 
gacgttaac cccgtgatg aagccgccc agaatctgt 
tcagtgccc ccgtcggtg aacagcgga ggtacggaa 
gttagcgaa gcaaaaaag aatctccta gacagcgac 
tcggttttt gcaggcgac gacgaatcc cgttcgttc 
gacttcgac atgccgccg taccgcaaa tcgcagacc 
ctcctggag ccggattac cgcggtaat cacacctac 
cctccagac cttcttccc ttcccgccc cgtatgtgc 
caggtgtcc ccggccggt agttccgcc tcgacggta 
gcagacaca tcccgttta ccagcacac cacggccca 
caatcgcca ctacacagt ccactgtac gactccaac 
accctgtac cgaactctt ccttactcc agatcgcaa 
tcgccgttc gtgggcccc cccgcccga gtgccccgt 
cagggctac gtgacgata ccccggcgc ccccggcag 
cggtggagc accgacacc cccaccacc tccgacgtg 
gaggagccc ctctacgac aacctgggg ctgcgcacc 
accgtggac ggcagctcg gctctgtcc ttgaacaag 
ctgggggag gccaccacc cccaagtcg atacggctc 
ttccccatg agcccctcc agctgcgac cccatcgcg 
gagaacgag ccgcccccc gcagccccc aagctgtct 
ccgtcgagc acgcagtgg gccaaggcg aacgccgag 
gcgttgagg agtcccgac aacaggagg aactcgctc 
ccagatgga gggaaggtg aaagtagcg ccggtccct 
ccgcctaaa cctaaaaaa aggacttcg acgggacca 
ctattcgaa gatgaaggt gaagacggg acagaagtg 
tgagaatgg ctcaaactg gtgcgcgca gcactggtg 
acttgttaa atagtggtg ctaaaagtg cggaccaag 
tgtctggac acagggttt atcggacga ttttttatg 
aaatttttc tgtgcctta acgacgaaa tcgatttaa 
agttctata gctggtgta tttagtgct tgacccttc 
gtgtgtgtt tcctacggg cttccatgg acgcgtgtg 
tcgtagcgc attttcgaa atatgatag agagggtgg 
agtgtccgt cgcagctag gaaagttat gatctcaaa 
accgtttgt gcatagaca aacagcgct gtgattgat 
ggcaccgca acagtatta ttaatttag ataaacaag 
agcttgatg cgcgctatc tctatcgtc tcggcgaaa 
tttctcaga aattgttgt agtggatga actgtgatc 
ttttgacag ttacaaatc ggttaggtt cagccagat 
gtaaagagc gctttcacg gcagtagag cctttatta 
gcctgcata cagtatcat tgtttatgc gtcattatg 
tcccttata ttgacttga gataatgga accgagcaa 
acgacggac gtgaacgcg ctcttcgag atcatacat 
acattcata gttcttgct ttttggtgt tccactttt 
taataagag acccaaacc taccctact tttcaaatc 
gtcgcttgt aattaacta caaaactat tattgtact 
atcaaattg tatatagtt acatatttt ctatgactg 
tactgtata ttcatttta aaatataga gctaagtgt 
aacgaaatc tacataaac gatcaataa aaagaatat 
tacagt
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