Status
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Gene information
General information
TC007110 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9250463..9268608
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007110
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04277

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva bent - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007110 RA
      MAHRLIVLM VLWSLASAN NDDWEKKCN KCKCVWSNG 
      KRTADCTNR DFSEIPKDL SSEIREIDF SNNPLHYLG 
      REVFVNAEL RDIHKLRFV NCSISAMDD TAFKGLVLL 
      IELDLSRNS IGLLTSKIF EENRKLRIL SLSHNKVKR 
      LDQGLFYNM THLQRLSLD HNEIEYIND SAFFLLPAL 
      QHLNLAYNK LTVMSSDFL DNFPKIVSL NLESNPWIC 
      DCRLQEFRN QTKRANLIT TQTQCAEPP LLKGRLWTD 
      DNIVFACVP QIVEPLPST HIEATTSNI TLTCRVLGD 
      PQPDVDWVS NGRIIDRDP RLNTQRFIT SKRKVGDYT 
      WNNLTITNV NYRDKGEYK CVAKNPGGS DEKNVSLIV 
      SSIGGIGGG GPLAFGATL PLVIALSIG GVIALVVIL 
      ILVCCCCRK STHGMATKR RDLQDSSDE CIRLHGQPD 
      MEKALITDV NPVMKPPRI CSVPPSVNS GGTEVSEAK 
      KNLLDSDSV FAGDDESRS FDFDMPPYR KSQTLLEPD 
      YRGNHTYPP DLLPFPPRM CQVSPAGSS ASTVADTSR 
      LPAHHGPQS PLHSPLYDS NTLYRTLPY SRSQSPFVG 
      PPARVPRQG YVTIPRRPR QRWSTDTPT TSDVEEPLY 
      DNLGLRTTV DGSSALSLN KLGEATTPK SIRLFPMSP 
      SSCDPIAEN EPPPAAPKL SPSSTQWAK ANAEALRSP 
      DNRRNSLPD GGKVKVAPV PPPKPKKRT STGPLFEDE 
      GEDGTEV
    CDS sequence
    • >TC007110 RA
      atggcacac cggcttata gtcctgatg gtgctctgg 
      agtctggca tctgcgaat aacgacgac tgggaaaaa 
      aagtgcaac aagtgcaag tgcgtttgg tcgaacggt 
      aaaaggaca gcagattgt acgaatcgc gattttagc 
      gaaataccg aaagatttg agttcggaa atccgcgaa 
      atagacttt tcgaataac cctttgcat tatttaggc 
      cgtgaagtg tttgtgaat gcggaactt agagacatt 
      cataagcta aggttcgtt aattgtagt ataagtgca 
      atggacgat actgccttc aagggccta gttttacta 
      atcgaactg gatttatcg cgcaattca atcgggctt 
      ttaacatcg aagatcttc gaagagaac cgcaaatta 
      aggatttta tccttgagc cacaataaa gtgaagagg 
      ttggaccag gggttgttt tacaatatg acacatctg 
      cagagatta tccttagat cataacgaa attgagtac 
      atcaatgac tcggcgttt ttccttctc ccagcgctc 
      cagcatctc aacttggct tacaacaag ctgacggtg 
      atgagttcc gactttttg gacaatttc ccgaaaatt 
      gtcagtttg aatctcgaa tccaacccc tggatctgc 
      gactgccgc ctccaggag ttccgaaat caaaccaaa 
      agggcaaat ttaataacg acccagacc cagtgtgcc 
      gagccgccc cttctgaag gggcggtta tggaccgat 
      gacaacatc gtcttcgct tgtgtgccc cagatcgtg 
      gagccgctc ccaagcacc cacatcgag gccaccacc 
      agcaacata accctcact tgccgcgtc ctgggcgac 
      ccccagccc gacgtcgac tgggtctcc aacgggcga 
      ataatcgac cgagacccc cgactcaac acccaacgc 
      ttcatcact tccaaacgc aaagtcgga gactacacg 
      tggaataat ttgacaata acaaacgtg aattaccgc 
      gacaagggc gaatataaa tgcgtcgcg aaaaatcca 
      ggaggttcc gacgagaaa aacgtcagt ttgatcgtc 
      tcctccatc ggggggatt gggggaggg ggcccgctg 
      gccttcggg gcgaccctc cccctcgtg atagcgttg 
      agtatcggc ggagtgatc gccctcgtg gtgatcctc 
      atcctcgtg tgctgctgc tgcaggaag agcacgcac 
      gggatggcg accaagagg agggacctg caggactcc 
      tccgacgag tgtataaga cttcacggg cagccggac 
      atggagaag gcgctcatt acggacgtt aaccccgtg 
      atgaagccg cccagaatc tgttcagtg cccccgtcg 
      gtgaacagc ggaggtacg gaagttagc gaagcaaaa 
      aagaatctc ctagacagc gactcggtt tttgcaggc 
      gacgacgaa tcccgttcg ttcgacttc gacatgccg 
      ccgtaccgc aaatcgcag accctcctg gagccggat 
      taccgcggt aatcacacc taccctcca gaccttctt 
      cccttcccg ccccgtatg tgccaggtg tccccggcc 
      ggtagttcc gcctcgacg gtagcagac acatcccgt 
      ttaccagca caccacggc ccacaatcg ccactacac 
      agtccactg tacgactcc aacaccctg taccgaact 
      cttccttac tccagatcg caatcgccg ttcgtgggc 
      ccccccgcc cgagtgccc cgtcagggc tacgtgacg 
      ataccccgg cgcccccgg cagcggtgg agcaccgac 
      acccccacc acctccgac gtggaggag cccctctac 
      gacaacctg gggctgcgc accaccgtg gacggcagc 
      tcggctctg tccttgaac aagctgggg gaggccacc 
      acccccaag tcgatacgg ctcttcccc atgagcccc 
      tccagctgc gaccccatc gcggagaac gagccgccc 
      cccgcagcc cccaagctg tctccgtcg agcacgcag 
      tgggccaag gcgaacgcc gaggcgttg aggagtccc 
      gacaacagg aggaactcg ctcccagat ggagggaag 
      gtgaaagta gcgccggtc cctccgcct aaacctaaa 
      aaaaggact tcgacggga ccactattc gaagatgaa 
      ggtgaagac gggacagaa gtgtga
    mRNA sequence
    • >TC007110 RA
      tatcaatgt tgaaaacgc attgaagct tatttactt 
      tgccttttc ttttgcagt tgagggttg caacatgcc 
      agacggaat ccgccagca gctctaaat taatataaa 
      cgttagcgt atgaagatg gcacaccgg cttatagtc 
      ctgatggtg ctctggagt ctggcatct gcgaataac 
      gacgactgg gaaaaaaag tgcaacaag tgcaagtgc 
      gtttggtcg aacggtaaa aggacagca gattgtacg 
      aatcgcgat tttagcgaa ataccgaaa gatttgagt 
      tcggaaatc cgcgaaata gacttttcg aataaccct 
      ttgcattat ttaggccgt gaagtgttt gtgaatgcg 
      gaacttaga gacattcat aagctaagg ttcgttaat 
      tgtagtata agtgcaatg gacgatact gccttcaag 
      ggcctagtt ttactaatc gaactggat ttatcgcgc 
      aattcaatc gggctttta acatcgaag atcttcgaa 
      gagaaccgc aaattaagg attttatcc ttgagccac 
      aataaagtg aagaggttg gaccagggg ttgttttac 
      aatatgaca catctgcag agattatcc ttagatcat 
      aacgaaatt gagtacatc aatgactcg gcgtttttc 
      cttctccca gcgctccag catctcaac ttggcttac 
      aacaagctg acggtgatg agttccgac tttttggac 
      aatttcccg aaaattgtc agtttgaat ctcgaatcc 
      aacccctgg atctgcgac tgccgcctc caggagttc 
      cgaaatcaa accaaaagg gcaaattta ataacgacc 
      cagacccag tgtgccgag ccgcccctt ctgaagggg 
      cggttatgg accgatgac aacatcgtc ttcgcttgt 
      gtgccccag atcgtggag ccgctccca agcacccac 
      atcgaggcc accaccagc aacataacc ctcacttgc 
      cgcgtcctg ggcgacccc cagcccgac gtcgactgg 
      gtctccaac gggcgaata atcgaccga gacccccga 
      ctcaacacc caacgcttc atcacttcc aaacgcaaa 
      gtcggagac tacacgtgg aataatttg acaataaca 
      aacgtgaat taccgcgac aagggcgaa tataaatgc 
      gtcgcgaaa aatccagga ggttccgac gagaaaaac 
      gtcagtttg atcgtctcc tccatcggg gggattggg 
      ggagggggc ccgctggcc ttcggggcg accctcccc 
      ctcgtgata gcgttgagt atcggcgga gtgatcgcc 
      ctcgtggtg atcctcatc ctcgtgtgc tgctgctgc 
      aggaagagc acgcacggg atggcgacc aagaggagg 
      gacctgcag gactcctcc gacgagtgt ataagactt 
      cacgggcag ccggacatg gagaaggcg ctcattacg 
      gacgttaac cccgtgatg aagccgccc agaatctgt 
      tcagtgccc ccgtcggtg aacagcgga ggtacggaa 
      gttagcgaa gcaaaaaag aatctccta gacagcgac 
      tcggttttt gcaggcgac gacgaatcc cgttcgttc 
      gacttcgac atgccgccg taccgcaaa tcgcagacc 
      ctcctggag ccggattac cgcggtaat cacacctac 
      cctccagac cttcttccc ttcccgccc cgtatgtgc 
      caggtgtcc ccggccggt agttccgcc tcgacggta 
      gcagacaca tcccgttta ccagcacac cacggccca 
      caatcgcca ctacacagt ccactgtac gactccaac 
      accctgtac cgaactctt ccttactcc agatcgcaa 
      tcgccgttc gtgggcccc cccgcccga gtgccccgt 
      cagggctac gtgacgata ccccggcgc ccccggcag 
      cggtggagc accgacacc cccaccacc tccgacgtg 
      gaggagccc ctctacgac aacctgggg ctgcgcacc 
      accgtggac ggcagctcg gctctgtcc ttgaacaag 
      ctgggggag gccaccacc cccaagtcg atacggctc 
      ttccccatg agcccctcc agctgcgac cccatcgcg 
      gagaacgag ccgcccccc gcagccccc aagctgtct 
      ccgtcgagc acgcagtgg gccaaggcg aacgccgag 
      gcgttgagg agtcccgac aacaggagg aactcgctc 
      ccagatgga gggaaggtg aaagtagcg ccggtccct 
      ccgcctaaa cctaaaaaa aggacttcg acgggacca 
      ctattcgaa gatgaaggt gaagacggg acagaagtg 
      tgagaatgg ctcaaactg gtgcgcgca gcactggtg 
      acttgttaa atagtggtg ctaaaagtg cggaccaag 
      tgtctggac acagggttt atcggacga ttttttatg 
      aaatttttc tgtgcctta acgacgaaa tcgatttaa 
      agttctata gctggtgta tttagtgct tgacccttc 
      gtgtgtgtt tcctacggg cttccatgg acgcgtgtg 
      tcgtagcgc attttcgaa atatgatag agagggtgg 
      agtgtccgt cgcagctag gaaagttat gatctcaaa 
      accgtttgt gcatagaca aacagcgct gtgattgat 
      ggcaccgca acagtatta ttaatttag ataaacaag 
      agcttgatg cgcgctatc tctatcgtc tcggcgaaa 
      tttctcaga aattgttgt agtggatga actgtgatc 
      ttttgacag ttacaaatc ggttaggtt cagccagat 
      gtaaagagc gctttcacg gcagtagag cctttatta 
      gcctgcata cagtatcat tgtttatgc gtcattatg 
      tcccttata ttgacttga gataatgga accgagcaa 
      acgacggac gtgaacgcg ctcttcgag atcatacat 
      acattcata gttcttgct ttttggtgt tccactttt 
      taataagag acccaaacc taccctact tttcaaatc 
      gtcgcttgt aattaacta caaaactat tattgtact 
      atcaaattg tatatagtt acatatttt ctatgactg 
      tactgtata ttcatttta aaatataga gctaagtgt 
      aacgaaatc tacataaac gatcaataa aaagaatat 
      tacagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY