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Gene information
General information
TC007103 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9475147..9478188
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007103
    iBeetle screen iB_04275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007103 RA
      MSAPKCSVA VCQFTATNN KENNLQIVK QLVSEAAQK 
      QAKIVFLPE ASDYIAANK NEAKAFAEP LNGTLMNEY 
      RNLAKTRKV WLSVGGFHE LVNEHQIFN THVLIDDEG 
      EIKSVYKKL HLFDVSIPE LNVNLRESD LNEAGRHLV 
      PPVMTPAGP LALAICYDL RFPELSIIQ RKQGANILT 
      YPSAFTKAT GALHWETLL RSRAIETQC YVIAAAQYG 
      KHNEKRTSY GQALIVDPQ GKIIAECPK YREGHETNQ 
      SIAIAEIDS NLIQKVRTE MPVFQHRRS DIYQLQVVG 
      GEKIPINDE ETFSFAHKI IPASTVFYR TRHCYAFTN 
      IRCVVPGHV LISTVRCCK RLEDLTSEE VTDLFLTAV 
      KVQKAVENE YSASSSTLC VQDGKHAGQ TIPHVHIHI 
      LPRKPNDFE VNDEIYDRL AKHDREESQ EPLRNVTEM 
      SQEADTLRK YFYQ
    CDS sequence
    • >TC007103 RA
      atgagtgcc ccgaaatgt agtgtggca gtttgccag 
      tttacagca acaaacaat aaggagaac aacttgcaa 
      atcgttaaa caattggtg tctgaagcg gcccagaaa 
      caagccaaa atagtattt ttacccgaa gcttccgac 
      tatatcgct gccaataaa aatgaggcc aaagcgttt 
      gcagaacca ctgaatgga acgttaatg aatgaatat 
      cgaaatttg gccaaaacc agaaaagtt tggttgtca 
      gtgggtggt tttcacgaa ttggtcaat gagcaccaa 
      atttttaat acacatgtg ttgattgac gacgagggc 
      gagataaaa tcagtgtac aaaaaattg catttgttt 
      gatgtcagc attccagag ttgaatgtt aacttgagg 
      gagtcagat ctcaatgag gcagggcgc caccttgtg 
      cccccggtt atgacccca gcagggcct ctagcttta 
      gccatatgt tacgatttg cgattccct gaactgagc 
      attatccaa cgcaaacaa ggcgccaat attttaaca 
      tacccttca gcgttcacc aaagcgaca ggagccctc 
      cattgggaa acacttcta cgatctaga gctattgaa 
      acccaatgt tacgttata gcggcagcg caatacggc 
      aaacataac gaaaaaaga acctcatat ggacaagcc 
      cttattgta gacccccaa ggaaagata atcgcagaa 
      tgcccaaaa tatcgcgaa gggcatgaa acgaaccaa 
      agcatagca attgcagaa attgactcg aacttaatt 
      cagaaagtt cggacagaa atgcccgtt tttcaacat 
      agacgcagc gatatttac caacttcag gtcgttgga 
      ggcgaaaaa ataccaatt aatgatgag gaaactttc 
      tcatttgct cataaaatt attccagcg agcacagtt 
      ttttacagg acgaggcat tgctacgct tttacaaac 
      atcagatgc gttgttcca ggacatgtt ttaatttca 
      acggtgcgt tgttgcaaa cgcttggag gatttaact 
      tcagaagaa gtaaccgat ttattttta acagccgtc 
      aaagttcag aaagctgtc gaaaatgaa tattcggcc 
      agttcttca acactttgt gtacaagat ggcaaacac 
      gcgggtcaa acaatacca cacgtccat atacacatt 
      ctaccgaga aaacccaac gattttgag gtaaatgat 
      gaaatttac gacagacta gccaaacat gatcgggaa 
      gagagccaa gaaccgctc agaaatgtc accgaaatg 
      tcccaggaa gcggacact ttacgaaaa tatttctat 
      caataa
    mRNA sequence
    • >TC007103 RA
      cctcataaa cgacaatac ggttatgtc aatcatttt 
      taaagccaa tttaattaa attgtgtcg tgtcactct 
      taatcaaaa ttacagaat gagtgcccc gaaatgtag 
      tgtggcagt ttgccagtt tacagcaac aaacaataa 
      ggagaacaa cttgcaaat cgttaaaca attggtgtc 
      tgaagcggc ccagaaaca agccaaaat agtattttt 
      acccgaagc ttccgacta tatcgctgc caataaaaa 
      tgaggccaa agcgtttgc agaaccact gaatggaac 
      gttaatgaa tgaatatcg aaatttggc caaaaccag 
      aaaagtttg gttgtcagt gggtggttt tcacgaatt 
      ggtcaatga gcaccaaat ttttaatac acatgtgtt 
      gattgacga cgagggcga gataaaatc agtgtacaa 
      aaaattgca tttgtttga tgtcagcat tccagagtt 
      gaatgttaa cttgaggga gtcagatct caatgaggc 
      agggcgcca ccttgtgcc cccggttat gaccccagc 
      agggcctct agctttagc catatgtta cgatttgcg 
      attccctga actgagcat tatccaacg caaacaagg 
      cgccaatat tttaacata cccttcagc gttcaccaa 
      agcgacagg agccctcca ttgggaaac acttctacg 
      atctagagc tattgaaac ccaatgtta cgttatagc 
      ggcagcgca atacggcaa acataacga aaaaagaac 
      ctcatatgg acaagccct tattgtaga cccccaagg 
      aaagataat cgcagaatg cccaaaata tcgcgaagg 
      gcatgaaac gaaccaaag catagcaat tgcagaaat 
      tgactcgaa cttaattca gaaagttcg gacagaaat 
      gcccgtttt tcaacatag acgcagcga tatttacca 
      acttcaggt cgttggagg cgaaaaaat accaattaa 
      tgatgagga aactttctc atttgctca taaaattat 
      tccagcgag cacagtttt ttacaggac gaggcattg 
      ctacgcttt tacaaacat cagatgcgt tgttccagg 
      acatgtttt aatttcaac ggtgcgttg ttgcaaacg 
      cttggagga tttaacttc agaagaagt aaccgattt 
      atttttaac agccgtcaa agttcagaa agctgtcga 
      aaatgaata ttcggccag ttcttcaac actttgtgt 
      acaagatgg caaacacgc gggtcaaac aataccaca 
      cgtccatat acacattct accgagaaa acccaacga 
      ttttgaggt aaatgatga aatttacga cagactagc 
      caaacatga tcgggaaga gagccaaga accgctcag 
      aaatgtcac cgaaatgtc ccaggaagc ggacacttt 
      acgaaaata tttctatca ataatgata aattgtaag 
      aaaattatg taaataatg cgggagtgg ttaataaaa 
      tattttttc aagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY