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Gene information
General information
TC007096 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9607927..9610121
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007096
    iBeetle screen iB_04274

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007096 RA
      MCRHKSAFS TRTKSNETN IIKSKSADI LIPNQLIHE 
      YVWEHLDKW YHKTAVICF DTTKSYTFG EIYHKSVAL 
      AGVLRNKFQ LNKGDTVAV VLPNVPDFP IVFLGTIQA 
      GLVVTTVNP YYTPDEIAA QLADSNSKL IFTINELVP 
      VINKCTKIL RRSIPTVLA SDNVPNECI KLSELCDTR 
      NSEIADFLN PDDVALLPY SSGTTGLSK GVQLTHKNV 
      VSNLYQMSS PDFVVNLET RSNFQDVIP VFLPLFHIY 
      GMVGIFLNF FAKGCKLIM VPTFVGPQF IKILQLYQP 
      TLLFAVPQM IVTILNNPK IKYDNLKSI RTIISAAAP 
      LGASAVDEF NKKCKNRIN LLQMYGMTE TSPLTLMQT 
      AKLQNGAKV GGSGFVIPN TEVKIISIS DNSTALGPN 
      QSGELVVRG PQNMKGYHN NPTATKKTI RNNWLRTGD 
      ISYYDEDQH FFITDRLKE LIKVKGFQV APAELEEIL 
      KSHPSVEDA AVVGIPHPV QGEAPKAFV VLKKEVRPE 
      LLKEFW
    CDS sequence
    • >TC007096 RA
      atgtgtaga cacaaaagt gcgttttca acacgaaca 
      aaatcaaac gaaacaaat ataataaaa tcgaaatcg 
      gctgatatt ttaatccca aatcaacta attcatgag 
      tacgtgtgg gagcattta gataaatgg tatcacaaa 
      accgctgtg atttgtttt gatactacg aaatcgtac 
      acttttggt gaaatttac cacaaaagc gttgcttta 
      gctggggtt ttgcgtaac aaattccaa ctgaataaa 
      ggagacact gtcgccgtt gtattacca aatgtacca 
      gactttccg atcgttttc ttaggaaca attcaagct 
      gggcttgtg gtcactaca gtaaaccct tattacacc 
      ccagatgaa atagccgcc caactcgcc gattcaaac 
      tcgaaacta atttttacc atcaacgaa ctcgtgcca 
      gtaataaat aaatgtaca aaaatactt aggagatcg 
      attccgacc gttttagcg agcgataat gtaccaaat 
      gagtgtatt aaattaagc gaattgtgt gatactaga 
      aacagtgaa atagcagac tttttgaac ccagatgac 
      gttgccctt ttgccctac tccagcggg accacaggt 
      ctgtctaaa ggtgtgcaa cttactcac aaaaacgtc 
      gtttcgaat ttataccaa atgtcgtcg cccgatttc 
      gtcgtcaat ttggagact cggagcaat ttccaggac 
      gtaatccca gttttcctc cctcttttc cacatctac 
      ggaatggta ggaattttc ctcaatttc ttcgccaag 
      ggttgcaaa ctgataatg gtaccaacg ttcgttggg 
      ccccaattt atcaaaatc ttgcagttg taccaacca 
      actttacta ttcgcggtc cctcaaatg attgttacg 
      atcttgaac aaccctaaa ataaaatac gacaatttg 
      aaaagtatc cgaacgatt atttcggct gcggcccct 
      cttggggca tcggcagtt gacgagttt aacaaaaaa 
      tgcaaaaat cggattaat ttgttacaa atgtacgga 
      atgaccgaa acgtcgccc ttgacgctc atgcaaacg 
      gcaaaactg caaaacggg gcgaaagtc ggaggcagc 
      ggctttgta ataccaaac accgaagtt aaaattatt 
      tcaatcagc gacaactca accgcttta gggccaaac 
      caatccggc gagttggta gtaagaggg ccacaaaac 
      atgaagggc taccacaac aatcccaca gcgacgaaa 
      aaaaccatt cgaaacaat tggttgcgg accggtgac 
      atcagctac tacgacgaa gaccaacat ttttttatc 
      accgataga ctgaaagaa ttgatcaaa gtgaaaggg 
      tttcaagta gctccagct gagcttgag gagatcctc 
      aaaagccac ccaagtgtt gaagatgcc gctgttgtg 
      ggaatcccc catcctgtg caaggggaa gcccctaaa 
      gcgtttgtg gtcttgaag aaagaagtg agacccgaa 
      cttttgaaa gaattttgg tag
    mRNA sequence
    • >TC007096 RA
      gaatttttg taaaatcat aatttccaa aattacaac 
      aaaatttaa aaaactatg caagcaaca ttacacaac 
      atgctcact tttttgtct tttcgaata atttatttt 
      gtggcatct gtgagtccg aaaaaacac tatcttact 
      gatcagtga ttattatac agattttta aattattaa 
      aaaaataaa atgaatggc atttaccat tttctgcac 
      ttctgacta atgacactt ttagcacat gacataatg 
      tttgttttt gatgcattg cataattca aacaaggtt 
      atgtttttg tcagaacaa tacaataat ttaatattt 
      ttttgcaca attttatga caacactaa aaaatgtac 
      attaggcga gcaatctgc aaattaaat aaaatgtgt 
      agacacaaa agtgcgttt tcaacacga acaaaatca 
      aacgaaaca aatataata aaatcgaaa tcggctgat 
      attttaatc ccaaatcaa ctaattcat gagtacgtg 
      tgggagcat ttagataaa tggtatcac aaaaccgct 
      gtgatttgt tttgatact acgaaatcg tacactttt 
      ggtgaaatt taccacaaa agcgttgct ttagctggg 
      gttttgcgt aacaaattc caactgaat aaaggagac 
      actgtcgcc gttgtatta ccaaatgta ccagacttt 
      ccgatcgtt ttcttagga acaattcaa gctgggctt 
      gtggtcact acagtaaac ccttattac accccagat 
      gaaatagcc gcccaactc gccgattca aactcgaaa 
      ctaattttt accatcaac gaactcgtg ccagtaata 
      aataaatgt acaaaaata cttaggaga tcgattccg 
      accgtttta gcgagcgat aatgtacca aatgagtgt 
      attaaatta agcgaattg tgtgatact agaaacagt 
      gaaatagca gactttttg aacccagat gacgttgcc 
      cttttgccc tactccagc gggaccaca ggtctgtct 
      aaaggtgtg caacttact cacaaaaac gtcgtttcg 
      aatttatac caaatgtcg tcgcccgat ttcgtcgtc 
      aatttggag actcggagc aatttccag gacgtaatc 
      ccagttttc ctccctctt ttccacatc tacggaatg 
      gtaggaatt ttcctcaat ttcttcgcc aagggttgc 
      aaactgata atggtacca acgttcgtt gggccccaa 
      tttatcaaa atcttgcag ttgtaccaa ccaacttta 
      ctattcgcg gtccctcaa atgattgtt acgatcttg 
      aacaaccct aaaataaaa tacgacaat ttgaaaagt 
      atccgaacg attatttcg gctgcggcc cctcttggg 
      gcatcggca gttgacgag tttaacaaa aaatgcaaa 
      aatcggatt aatttgtta caaatgtac ggaatgacc 
      gaaacgtcg cccttgacg ctcatgcaa acggcaaaa 
      ctgcaaaac ggggcgaaa gtcggaggc agcggcttt 
      gtaatacca aacaccgaa gttaaaatt atttcaatc 
      agcgacaac tcaaccgct ttagggcca aaccaatcc 
      ggcgagttg gtagtaaga gggccacaa aacatgaag 
      ggctaccac aacaatccc acagcgacg aaaaaaacc 
      attcgaaac aattggttg cggaccggt gacatcagc 
      tactacgac gaagaccaa cattttttt atcaccgat 
      agactgaaa gaattgatc aaagtgaaa gggtttcaa 
      gtagctcca gctgagctt gaggagatc ctcaaaagc 
      cacccaagt gttgaagat gccgctgtt gtgggaatc 
      ccccatcct gtgcaaggg gaagcccct aaagcgttt 
      gtggtcttg aagaaagaa gtgagaccc gaacttttg 
      aaagaattt tggtagttc tggaaagaa ttccccgaa 
      attgcgcgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY