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Gene information
General information
TC007092 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9681090..9689041
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007092
    iBeetle screen iB_04273

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007092 RA
      MAEVKPLYS TCVAVLGFI CFVVGATAV GIPMWGYFD 
      NLGGHGNEE RGYFGPWKT CKLLLYNRE RCGNDSSNF 
      RTTIAVWVA GVVAAIGVT ILGIFCFLS VIQLAMLSS 
      KEKVMMKYS IVVATKVAL GLLATLLAI AAAGLFALQ 
      ADDRRRTGF EISRGPGFY IQILLIVLT VILFAMSVY 
      DVLLSRREG GDPTRVAVP MEGATFGNP GFRERTNGR 
      SVSVTDASG KPYMPTASN GSMASMNTT STTIGSTNG 
      STVGSSVTR SPLRSSLKK PKPKDGLGI QNPGFSGTS 
      PTLSRNGST KKVRIQTHS TEV
    CDS sequence
    • >TC007092 RA
      atggcggaa gttaaaccc ttgtattcg acatgtgtt 
      gccgttttg ggatttatc tgtttcgta gttggcgcc 
      actgccgtc ggaataccc atgtggggg tatttcgat 
      aatcttggt ggacacggc aacgaagaa cggggatat 
      ttcgggcct tggaagact tgcaaactt cttctgtac 
      aacagggag aggtgcggg aatgactcg tcaaatttc 
      cgaaccaca attgcggtt tgggtggct ggggtggtg 
      gccgcaatc ggagtgaca attttgggg atcttctgc 
      tttctgagt gtcatccag ctggcaatg ttgagttcg 
      aaggaaaaa gtgatgatg aaatacagc atagtcgtc 
      gcaacgaaa gtagcgtta ggactccta gcgacttta 
      ttggcgatc gcagcagct ggtctcttt gcccttcaa 
      gccgacgac cgacgacgc actggcttt gagattagc 
      agagggccc ggattttat attcagatt ttgttgata 
      gtcttaacg gtgatactg tttgcgatg tcggtgtat 
      gacgtcctg ctctcgagg agagagggc ggagacccc 
      acgagggtt gcagtgcca atggaaggt gccaccttt 
      gggaatccc ggctttcgg gagcgaacc aacgggcga 
      agcgtttcg gtgacggac gccagcggt aagccctac 
      atgcccacg gcttccaac gggagcatg gcctcgatg 
      aacaccact tcgacgacc attgggtcc acaaacggg 
      tcaactgtg gggtcgtca gtaacgcgg tcgccgctg 
      cggtccagt ttgaaaaag ccgaagccc aaggacggg 
      cttggcatt caaaatccg ggcttttcc gggacgtcg 
      cccacgctc tcccggaat gggagcacg aaaaaagtt 
      cggatacag acccacagt actgaagtg tag
    mRNA sequence
    • >TC007092 RA
      agctcgaaa catgttact ggccttagt tggattaaa 
      accgcgtaa taaagtcga gcgagcgcc tctttccca 
      ttaaatttt ccccaaaaa tgagggaaa acggcgcaa 
      cgtcagccg cgaaaatcg aattttcca agacgtgga 
      aattgtaaa caagtgtat tgattttgg gtgtcgcgt 
      gtatgattg tgttattcc gacgtcatg cgcggtgtt 
      ggtgttgtt tttcttaaa acgtggtga ttttgtgga 
      taattcaac gacagatca tggttgctt tatgatatg 
      gcggaagtt aaacccttg tattcgaca tgtgttgcc 
      gttttggga tttatctgt ttcgtagtt ggcgccact 
      gccgtcgga atacccatg tgggggtat ttcgataat 
      cttggtgga cacggcaac gaagaacgg ggatatttc 
      gggccttgg aagacttgc aaacttctt ctgtacaac 
      agggagagg tgcgggaat gactcgtca aatttccga 
      accacaatt gcggtttgg gtggctggg gtggtggcc 
      gcaatcgga gtgacaatt ttggggatc ttctgcttt 
      ctgagtgtc atccagctg gcaatgttg agttcgaag 
      gaaaaagtg atgatgaaa tacagcata gtcgtcgca 
      acgaaagta gcgttagga ctcctagcg actttattg 
      gcgatcgca gcagctggt ctctttgcc cttcaagcc 
      gacgaccga cgacgcact ggctttgag attagcaga 
      gggcccgga ttttatatt cagattttg ttgatagtc 
      ttaacggtg atactgttt gcgatgtcg gtgtatgac 
      gtcctgctc tcgaggaga gagggcgga gaccccacg 
      agggttgca gtgccaatg gaaggtgcc acctttggg 
      aatcccggc tttcgggag cgaaccaac gggcgaagc 
      gtttcggtg acggacgcc agcggtaag ccctacatg 
      cccacggct tccaacggg agcatggcc tcgatgaac 
      accacttcg acgaccatt gggtccaca aacgggtca 
      actgtgggg tcgtcagta acgcggtcg ccgctgcgg 
      tccagtttg aaaaagccg aagcccaag gacgggctt 
      ggcattcaa aatccgggc ttttccggg acgtcgccc 
      acgctctcc cggaatggg agcacgaaa aaagttcgg 
      atacagacc cacagtact gaagtgtag tgctgaact 
      tgattattt atttgtcat aatgaacaa gtacaaaca 
      cccaattgg cttaagtgc acttatttt atgaaggaa 
      gcgagtgaa atgtatata aagatatga agtacatga 
      cgttgtaaa tatctaagc tacttattt acacttatt 
      tttattccg tccagcaac aaagttgtg ttttttatt 
      ttgtgttta tagtttata tattttatt gtaaccgtt 
      tgtgaaacg gagaaattt tgacatatt ttattattg 
      ttatttcga tgttttatg ggttgggta tgtcactgg 
      aaaagtcta ttgctttaa ctagaacta ggactaatc 
      gaacttagt tttgtaaat agtccctcc ctccaaaat 
      tccgggctt ttatttgat gtactttat cataacttt 
      ttgtgatac taaatagag ctgtatcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY