| General information | |||
| TC007082 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 9928410..9942520 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007082 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04271 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
- >TC007082 RA
MCSKEQITE IMDKIHMGL PKCSVKLPA WENITNALP RFHIPAMPA VRSKRGWCG CLQDDEPPE ITYCVVENT GTLTLQAIT PTQPMPSQE ELDAKFAEL VEELDLTAP NKAAMLSLP SQKKWQIYC SRKGGEDTV DQTHAPEHY IERLRTLAT LQYPEANTE EEVRTRTKQ IDGLKTALR TSTHSFVIK FIELDGLSA LLDCLERMD YFTAQSSIH TSIIGCVKA LMNNSTGRA HVLAHPTSI NTIAQSLST ENIKTKIAA LEILGAVCL VSGGHKKVL DAMLHYQKY AFERTRFQG IINDLDRST GIYRDEVNL KTAIMSFVN AVLNYGQGQ ENLEFRLHL RYEFLMLGI QPIIDKLRS HENETLDRH LDFFEMVRN EDEKELARK FELEHVDTK SATAMFDLI RRKLSHTAA YPHLLSLLE HCLLLPLDY GSHPQHWLL FDRIVQQIV LQQDNDVKN PDVSPIDIN VKEIVHLLA KEEELTEAR KKAEELERE NTDMSNKLA KKEQELDQR TQEKEDIES SLVRIKERL EKETAAHIE TQQRLNELE YRAADLDRQ VSCERGERF RLERLVSSG SIPDDAKVQ GLKSTVDVE YKSPPPPPP LAPPPPPPA PGPPPPPNA PMAPPPEPF KVETVKKNI PQPANPLKS FNWSKLPET KLAGTIWSE LDDTKLYNT MELDCIDKL FSAYQKNGV TNDGSIEDL RQMGKNRTK VLSVIDSRR AQNCTILLS KLKMSDEDI TKAILSMDC KEQLPIDMV EQLLKFTPS SEEAALLEE HSDEIDSLA RADRFLYEI SKVPHYEQR LRSLHYKKR FQVTLNEII PRITSVMEA SREVSRSRR LRRLLEIVL ALGNYMNRG ARGNASGFR LASLNRLAD TKSSAAKGT TLLHYLVQI IEKKFKDIL RLDEDIPHV RVAAKVSLG ELSKDMAQL RLGLKDVAK EIEFHLSQS PLANDKFVP VMREFQATA TCRLAEVED QYQDMKTRF ERAVRLFGE DPTNAQPDE FFGVFDAFL TSFTEARQD NENMRRRQE EEEKRAKQE AELKKLTLE RKHSREGIL SSINKSLSL KNGESAKGE FDDLISALR TGDVFGEDM AKFKRSRKS RVTGNSPPR RNSVNREDS RERIVSSGR RQ
- >TC007082 RA
atgtgcagc aaggagcaa atcaccgaa attatggat aaaattcac atggggttg cccaagtgt tcggtcaaa ctgcctgct tgggaaaac ataactaac gccctgcct cgattccac ataccagcc atgccggcc gtgcgatcc aagcgcggc tggtgcggc tgtcttcaa gatgacgaa ccccctgaa ataacctac tgcgtggtg gagaacacc ggaaccctc actcttcag gccatcacc ccgacacaa ccaatgccc tcgcaggaa gaactggat gccaaattt gctgaactt gtggaagag ctcgacttg acagcccct aataaggct gccatgttg agtttgccg tcgcagaaa aaatggcaa atatattgc tcaagaaaa ggaggagaa gacaccgtg gaccaaacg cacgcacct gaacactac atcgaaaga ctgcgaact ttagcaaca ttgcaatac cctgaagcc aacactgag gaagaagtg aggacacgg acgaaacaa atcgatggc ttgaaaacg gcacttcgg acgtcaacg cacagtttt gtaataaaa tttatcgaa ttagatggc ctgtcggca cttttagac tgtttggaa cgtatggac tatttcacg gctcaaagc tcgattcat acgagcata attggttgt gttaaagct ctgatgaac aattcgact gggcgagct cacgttttg gcgcacccg acaagcatc aacaccata gctcagtcc ctaagcact gaaaatatc aaaacaaaa atcgcagct cttgaaatt ttgggtgcg gtttgtttg gtgtcgggt gggcataag aaggtgtta gatgcaatg cttcattac caaaaatat gcgttcgaa aggacaagg tttcaagga atcatcaac gatttggat cgctcgact ggaatctac cgcgatgag gttaatttg aaaaccgct ataatgtca ttcgttaat gctgtactg aactatggc caagggcaa gagaactta gaatttagg ttacacctc cgatacgag tttctcatg ctgggtatt cagccaatc atcgataaa ttgcgaagt cacgagaat gagacgctg gaccgccac ttggatttt ttcgaaatg gtgcgaaac gaagacgag aaggagctg gctcggaaa ttcgagctg gagcatgtt gataccaaa agtgcaacc gcaatgttt gatctcatt agacgtaaa ttaagccac actgcggcc tacccgcac ctgctgtcc ctcctagag cactgtctc ctgctcccc ttggactac ggctcccac ccccaacac tggctcctc ttcgaccgc atcgtccaa caaatcgtc ctccaacaa gacaacgac gtcaaaaac cccgacgtt tcccccata gacatcaac gtcaaagaa atcgtccac ctcctcgca aaagaagaa gaactgacg gaagcgcgg aaaaaagcc gaggagctc gaacgcgag aacacagac atgtcaaac aaacttgcg aagaaagaa caagagctc gaccaacgc acccaggaa aaagaggat attgagtcg agcttagtc cggattaaa gaacgtctc gaaaaggaa acagcggcg catattgaa acccaacaa cgtttgaac gaactcgaa tatagagct gcagatttg gacagacaa gtgtcatgt gaaaggggt gagagattc cggttggag cgtttggtc agctcgggg agtattcca gacgatgcc aaagtgcaa ggtttgaaa tcgacggtg gatgtggag tataagagt ccaccgccg ccgccacca ttggcaccg ccgccgcct ccaccggcc cccgggccg ccaccgcca ccgaatgcc cctatggcg ccgccaccg gaacccttc aaggttgaa acagtcaag aaaaatatt ccacaaccg gcgaatcct ttaaaatca ttcaattgg tcgaaattg cctgaaact aagctagca gggacgatt tggagcgag ttggatgat actaagttg tacaatacg atggagttg gattgtatt gataaatta ttctcggcg tatcagaag aatggagtg acgaacgac ggttcgatt gaggatttg cgccagatg ggcaagaac cgaaccaaa gtattatca gtgatagac agccgtcgt gcccaaaac tgcaccatc ctgctctcg aaactcaaa atgtccgac gaagacatc actaaagcc atcttgagc atggactgc aaagagcaa ctcccaatc gacatggtc gagcaactc ttgaaattt actccaagt tccgaagaa gcagctctt ttggaagaa cacagcgac gaaatcgac tcgttggcc cgcgccgac cgcttccta tacgaaatt tccaaggtg cctcactat gaacaaaga ctccgcagc ttgcactac aagaaacgg ttccaagtc accctcaac gagatcatt ccacgcatc actagcgta atggaggcc tcccgcgag gtgtcccgc tcccgtcgt cttcgacgg cttttggaa atagtttta gcgttagga aactacatg aatcgaggg gctagaggg aacgcttcc ggcttccgt ctcgcgtca ctaaataga cttgctgat accaaatca agtgccgcc aaaggcacg acgttgctt cattatcta gtacaaata atcgagaag aaattcaaa gatatactg cgtttagac gaggatatt ccacatgtt agagtagca gccaaagtg tccttaggc gagcttagc aaggatatg gcgcagctg agactagga ttgaaggac gtagcgaaa gaaattgaa tttcatcta agtcagagt cctttagct aacgataag ttcgttcct gtcatgagg gagttccaa gctacggcc acgtgccga ctggctgaa gtcgaagac cagtatcaa gacatgaag acgaggttt gagcgcgca gttcgcctc tttggcgaa gacccgacg aacgcccaa cccgacgaa ttcttcggc gttttcgac gcgttttta acctcgttc actgaagcc cgccaagac aacgaaaac atgcgccgc cgacaggag gaggaggaa aagcgagcg aaacaagaa gcggagttg aagaaactc acccttgag cgaaagcac agtcgggag ggaattctc tcatcgatt aataaaagt ttgagtctg aaaaatggg gaaagtgcg aagggagaa ttcgacgat ttaatttcg gccttaaga acgggcgat gttttcggc gaagatatg gcaaagttt aaaaggtcg aggaagagt cgggtgact ggaaattcg ccaccgagg cggaattca gtcaatcgg gaagacagc agagaaaga attgttagt agcggaagg aggcagtaa
- >TC007082 RA
gctcgtgtt actaactct ggtgtagtt cacctgttg tgttcagtg aacgcgctg ctgcgctcc aagaaatgt caaacgcgc gatagtttc aaaaaatga accggcgtg aaatactta tttagtttt ttctacagt tttatacag aacagtgtg gtttttggg tgggttcaa acgccaaaa tgtgcagca aggagcaaa tcaccgaaa ttatggata aaattcaca tggggttgc ccaagtgtt cggtcaaac tgcctgctt gggaaaaca taactaacg ccctgcctc gattccaca taccagcca tgccggccg tgcgatcca agcgcggct ggtgcggct gtcttcaag atgacgaac cccctgaaa taacctact gcgtggtgg agaacaccg gaaccctca ctcttcagg ccatcaccc cgacacaac caatgccct cgcaggaag aactggatg ccaaatttg ctgaacttg tggaagagc tcgacttga cagccccta ataaggctg ccatgttga gtttgccgt cgcagaaaa aatggcaaa tatattgct caagaaaag gaggagaag acaccgtgg accaaacgc acgcacctg aacactaca tcgaaagac tgcgaactt tagcaacat tgcaatacc ctgaagcca acactgagg aagaagtga ggacacgga cgaaacaaa tcgatggct tgaaaacgg cacttcgga cgtcaacgc acagttttg taataaaat ttatcgaat tagatggcc tgtcggcac ttttagact gtttggaac gtatggact atttcacgg ctcaaagct cgattcata cgagcataa ttggttgtg ttaaagctc tgatgaaca attcgactg ggcgagctc acgttttgg cgcacccga caagcatca acaccatag ctcagtccc taagcactg aaaatatca aaacaaaaa tcgcagctc ttgaaattt tgggtgcgg tttgtttgg tgtcgggtg ggcataaga aggtgttag atgcaatgc ttcattacc aaaaatatg cgttcgaaa ggacaaggt ttcaaggaa tcatcaacg atttggatc gctcgactg gaatctacc gcgatgagg ttaatttga aaaccgcta taatgtcat tcgttaatg ctgtactga actatggcc aagggcaag agaacttag aatttaggt tacacctcc gatacgagt ttctcatgc tgggtattc agccaatca tcgataaat tgcgaagtc acgagaatg agacgctgg accgccact tggattttt tcgaaatgg tgcgaaacg aagacgaga aggagctgg ctcggaaat tcgagctgg agcatgttg ataccaaaa gtgcaaccg caatgtttg atctcatta gacgtaaat taagccaca ctgcggcct acccgcacc tgctgtccc tcctagagc actgtctcc tgctcccct tggactacg gctcccacc cccaacact ggctcctct tcgaccgca tcgtccaac aaatcgtcc tccaacaag acaacgacg tcaaaaacc ccgacgttt cccccatag acatcaacg tcaaagaaa tcgtccacc tcctcgcaa aagaagaag aactgacgg aagcgcgga aaaaagccg aggagctcg aacgcgaga acacagaca tgtcaaaca aacttgcga agaaagaac aagagctcg accaacgca cccaggaaa aagaggata ttgagtcga gcttagtcc ggattaaag aacgtctcg aaaaggaaa cagcggcgc atattgaaa cccaacaac gtttgaacg aactcgaat atagagctg cagatttgg acagacaag tgtcatgtg aaaggggtg agagattcc ggttggagc gtttggtca gctcgggga gtattccag acgatgcca aagtgcaag gtttgaaat cgacggtgg atgtggagt ataagagtc caccgccgc cgccaccat tggcaccgc cgccgcctc caccggccc ccgggccgc caccgccac cgaatgccc ctatggcgc cgccaccgg aacccttca aggttgaaa cagtcaaga aaaatattc cacaaccgg cgaatcctt taaaatcat tcaattggt cgaaattgc ctgaaacta agctagcag ggacgattt ggagcgagt tggatgata ctaagttgt acaatacga tggagttgg attgtattg ataaattat tctcggcgt atcagaaga atggagtga cgaacgacg gttcgattg aggatttgc gccagatgg gcaagaacc gaaccaaag tattatcag tgatagaca gccgtcgtg cccaaaact gcaccatcc tgctctcga aactcaaaa tgtccgacg aagacatca ctaaagcca tcttgagca tggactgca aagagcaac tcccaatcg acatggtcg agcaactct tgaaattta ctccaagtt ccgaagaag cagctcttt tggaagaac acagcgacg aaatcgact cgttggccc gcgccgacc gcttcctat acgaaattt ccaaggtgc ctcactatg aacaaagac tccgcagct tgcactaca agaaacggt tccaagtca ccctcaacg agatcattc cacgcatca ctagcgtaa tggaggcct cccgcgagg tgtcccgct cccgtcgtc ttcgacggc ttttggaaa tagttttag cgttaggaa actacatga atcgagggg ctagaggga acgcttccg gcttccgtc tcgcgtcac taaatagac ttgctgata ccaaatcaa gtgccgcca aaggcacga cgttgcttc attatctag tacaaataa tcgagaaga aattcaaag atatactgc gtttagacg aggatattc cacatgtta gagtagcag ccaaagtgt ccttaggcg agcttagca aggatatgg cgcagctga gactaggat tgaaggacg tagcgaaag aaattgaat ttcatctaa gtcagagtc ctttagcta acgataagt tcgttcctg tcatgaggg agttccaag ctacggcca cgtgccgac tggctgaag tcgaagacc agtatcaag acatgaaga cgaggtttg agcgcgcag ttcgcctct ttggcgaag acccgacga acgcccaac ccgacgaat tcttcggcg ttttcgacg cgtttttaa cctcgttca ctgaagccc gccaagaca acgaaaaca tgcgccgcc gacaggagg aggaggaaa agcgagcga aacaagaag cggagttga agaaactca cccttgagc gaaagcaca gtcgggagg gaattctct catcgatta ataaaagtt tgagtctga aaaatgggg aaagtgcga agggagaat tcgacgatt taatttcgg ccttaagaa cgggcgatg ttttcggcg aagatatgg caaagttta aaaggtcga ggaagagtc gggtgactg gaaattcgc caccgaggc ggaattcag tcaatcggg aagacagca gagaaagaa ttgttagta gcggaagga ggcagtaat ttttttgct cattattac agcttttgg atatttgtt gtgctactt catatttaa cggtgccct ggcctaaat gtgtttatt tctgttttt ttgtgatcg attttgtaa taattggac caatactta cgatgtaag caattgata tatttttta ttatactag tgctgccaa ataagcttc acaattttt atctagatt atcgtttgt attatatta acaccaatg tatggattt tatacatta taacaagat tgtatgttt gtatgacgt agctgtagt ccatgttta tatattgat taaggttta gacagattg tgctctgca ttgaaatgg aactgatta tacatttta atgttaaat atactattg ttaattta
